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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for 2 Chloro 1 1 trifluoromethyl 1 3 4 9 tetrahydro 2H beta carbolin 2 yl ethan 1 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: 2–2.5×106 cells resuspended in a mix of 1:1 PBS and Geltrex Basement Membrane Mix (Thermo Fisher Scientific) were subcutaneously injected in the right flank of 10–12 weeks old NOD/Scid/gamma (NSG ...
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bioRxiv - Neuroscience 2020Quote: ... After 5 days the spheroids were transferred to Neural differentiation medium (NdM) (Neurobasal, 2% B27, 1% GlutaMAX, 1% P/S (all from Gibco) supplemented with 20 ng/mL of EGF and FGF2 (both PrepoTech ...
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bioRxiv - Bioengineering 2022Quote: ... with 50 µL per injection site of vaccine formulations containing 5 µg of SARS-CoV-2 antigen (RBD-SD1, S-2P, S-PCNA1, or 6RBD-np) gently mixed 1:1 (v/v) with AddaVax (Invitrogen) adjuvant ...
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bioRxiv - Cancer Biology 2022Quote: ... using base medium (1:1 ratio of DMEM/F12 Glutamax/Neurobasal medium [Gibco], 2% B27 supplement without vitamin A [Gibco] ...
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bioRxiv - Developmental Biology 2022Quote: ... WIK 24hpf samples were spiked with 2 μl of 1:100 diluted ERCC spike-in mix 1 (4456740, Thermofisher Scientific) while all other samples were spiked with 2 μl of 1:100 diluted ERCC spike-in mix 2 (4456740) ...
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bioRxiv - Neuroscience 2020Quote: ... and equally distributed in wells containing growth medium (DMEM-F12-glutamax [Gibco], 40 μg/mL insulin [Sigma], 1/200 B-27 supplement [Gibco], 1/100 N-2 supplement [Gibco] ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 2-hour incubation with secondary antibody (1:200 Alexa 555 anti-rabbit [Invitrogen]; 1:200 Alexa 488 anti-chicken [Invitrogen]). Following three additional 10-min washes in PBST ...
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bioRxiv - Neuroscience 2021Quote: ... 6-diamino-2-phenylindole (DAPI, 1:25,000) for 1 min and the sections were mounted in ProLong® Gold antifade reagent (Invitrogen).
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bioRxiv - Microbiology 2021Quote: ... then covered with 2 mL overlay medium of 2% Avicel CL-611 (DuPont Pharma Solutions) diluted 1:1 with 2x minimum essential media (Gibco). The cells were then incubated for three days ...
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bioRxiv - Cell Biology 2021Quote: ... was added for 2 days (Day 1 and Day 2 of differentiation) followed by a neural induction period in Neural Induction Media (Advanced DMEM/F12 (1:1, Gibco), 1% non-essential amino acids (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were captured on the magnet and the supernatant now containing the dissociated cells was transferred to a 15 ml Falcon tube containing 10 ml ice cold FACS buffer (DMEM w/o phenol red + 1% P/S, 2% FBS, 1 mM EDTA pH 8, all from Gibco) and centrifuged for 5 minutes at 300 g and 4°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A total of 2 x 106 cells (ratio 1:1) were resuspended in a final volume of 100 uL in DMEM (Invitrogen) and injected into the hypodermis of the mouse skin ...
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bioRxiv - Microbiology 2022Quote: ... Mice were sacrificed at 1 dpi and lungs were collected in 1 mL DMEM/2% FBS/1X Antibiotic-Antimycotic (Gibco). Samples were homogenized and debris was clarified at maximum speed for 10 minutes at 4°C ...
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bioRxiv - Microbiology 2023Quote: Sorted cells were cultured in 2 ml RPMI 5.5 mM glucose (1:1 mixture of Cat. No. 11875093 and Cat. No. 11879020 [Thermo-Fisher Scientific]) supplemented with 20% FCS (v/v) ...
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bioRxiv - Neuroscience 2023Quote: ... Dissociated cells were suspended in culture medium (Neurobasal medium, 2% B27, 1% GlutaMAX™, GIBCO, 1% antibiotic/antimycotic (ThermoFisher Scientific)) supplemented with mouse NGF (50ng/μl ...
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bioRxiv - Immunology 2024Quote: ... HeLa (CCL-2, ATCC), COS-1 (CRL-1650, ATCC) and L-929 (CCL-1, ATCC) cells were maintained in DMEM (11995073, Gibco) supplemented with 1% Penicillin-Streptomycin (15140122 ...
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bioRxiv - Developmental Biology 2023Quote: ... The culture medium consisted of a 1:1 mixture of DMEM with 2 mM l-glutamine and F12 (Ham’s Nutrient Mix) (both from Life Technologies) and was supplemented with 10% (vol/vol ...
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bioRxiv - Immunology 2023Quote: The whole blood samples were 1:1 diluted with 2% fetal bovine serum (FBS) (Hyclone, SH30406.05) in phosphate buffered saline (PBS) (Invitrogen, C10010500BT) and subjected to Ficoll (Cytiva ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated in culture media (66% BME, 25% Hanks, 5% FBS, 1% N-2, 1% penicillin, streptomycin and glutamine; all from Invitrogen) and 0.66% D-(+)-Glucose (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... The base medium was gradually transitioned to N2/B27 medium (Neurobasal medium, 2% B27 supplement, 1% N2 supplement, 1% Glutamax, (ThermoFisher)) in 25% increments ...
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bioRxiv - Microbiology 2023Quote: ... were coated with 100 µL of recombinant SARS-CoV-2 S protein (Wuhan-1 strain and BA.5) at a concentration of 1 µg/mL in PBS (Gibco) at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated in PBS for 1 hour at room temperature and incubated 2 hours at room temperature with the appropriated Alexa488 (1/400, Invitrogen), Alexa594 (1/400 ...
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bioRxiv - Neuroscience 2023Quote: ... and re-plated on Matrigel-coated dishes in NPC Medium (DMEM/F12, 2% B27, 1% N2, 1% Pen-strep; all from Gibco) supplemented with 20 ng/mL bFGF (Peprotech) ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... Sections were washed three times in TBS for 10 min each before incubation for 1-2 h at RT with the following Alexa Fluor secondary antibodies made in goat (1:500; Invitrogen): anti-rabbit Alexa 488 (A11008) ...
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bioRxiv - Cell Biology 2023Quote: ... Counter-staining cell nuclei with a DNA-binding dye 4′,6-diamidino-2-phenylindole (DAPI, 1 μg ml−1 in PBS) and F-actin with Alexa Fluor dye conjugated phalloidin (Invitrogen) was also done at this step ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was changed to neural induction media (1:1 mixture of DMEM/F-12 GlutaMAX: Neurobasal media supplemented with 0.5x N-2 (Life Technologies), 0.5x B-27 (Life Technologies) ...
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bioRxiv - Biophysics 2023Quote: ... the purified DNA in 1 ml NaHCO3 (pH 8.3) was incubated with 1 mM SM(PEG)2 (Thermo Scientific Pierce) for 20 mins at 20-22°C ...
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bioRxiv - Cancer Biology 2023Quote: BE(2)-C (ATCC, CRL-2268) cells were cultured in a 1:1 mixture of DMEM/F12 (with HEPES, Gibco) and EMEM (ATCC) ...
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bioRxiv - Cell Biology 2023Quote: ... were grown in high-glucose DMEM with supplements (1% penicillin/streptomycin, 10% FBS, 1% Non-essential Amino Acids [NEAA; Wako], 55 µM 2-Mercaptoethanol [Gibco]). Mouse embryonic stem (ES ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 Nucleocapsid antibody (produced in mouse) was procured from (MA5-35943/Invitrogen/Massachusetts, USA/1:1000-1:10,000) used as primary antibody ...
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bioRxiv - Microbiology 2023Quote: ... buffer (1× PBS containing 2% FBS) followed by incubation with Alexa Flour 633 goat anti-human IgG (1: 2000 dilution, Invitrogen). Finally ...
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bioRxiv - Cell Biology 2024Quote: ... plasmid (EV or HLA-A2/Kb; 1 µg/µL) complex at 2:1 ratio prepared in Opti-MEM (Gibco, UK) for 18 hours in the presence or absence of 100 ng/mL type I IFNα (PBL Assay Science ...
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bioRxiv - Neuroscience 2024Quote: ... Cell cultures underwent half-media changes with serum-free media (Neurobasal Medium, 2% B27 supplement, 1% 200 mM L-glutamine, and 1% penicillin/streptomycin; all Gibco) two hours post-plating and maintained every 3-4 days for a total culturing period of 21 days ...
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bioRxiv - Neuroscience 2024Quote: ... and cells were resuspended in plating media (Neurobasal medium, 2% B27 supplement, 1% 200 mM L-glutamine, 1% penicillin/streptomycin (Gibco), 5% Fetal bovine serum (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg total RNA was mixed with 2 μl 1:100 diluted ERCC RNA Spike-In Mix (Thermo Fisher Scientific). Libraries were prepared with the NEBNext Ultra II Directional RNA Kit (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... We treated them with a 1:1 mixture of conditioned medium and normal culture medium (DMEM with high glucose [Sigma], 2% fetal bovine serum [ThermoFisher Scientific] ...
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bioRxiv - Microbiology 2024Quote: ... 1% w/v BSA) were precomplexed with anti-human Fc specific antibodies-HRP (A18817, ThermoFisher Scientific, 2:1 ratio lectin:antibody) and pre-incubated with 20 mM EDTA or 40 mM glucose ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were dissociated to single cells with Accutase supplemented with 10 µM Y-27632 and replated in neural maintenance medium (Neurobasal Plus, supplemented with 2% B27+ supplement, 1% Pen/Strep, 1% GlutaMax (all ThermoFisher) supplemented with 10 ng/mL BDNF and 5 ng/mL GDNF (both R&D Systems) ...
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bioRxiv - Biochemistry 2024Quote: ... pH 7.4, 10 mM KCl, 2 mM MgCl2, 1 mM EDTA, 1 mM EGTA with protease and phosphatase inhibitors Thermo Scientific, Waltham ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then dried at 100°C and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Cell Biology 2024Quote: ... The flow-through was then dried in a speed-vac and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: ... DNA and F-actin were counterstained for 20 min in the dark with 1 μL/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen) and Alexa Fluor 647 phalloidin (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... then the lysate equivalent to 2×105 cells was mixed at a 1:4 ratio with 4x LDS buffer containing 10% sample reducing agent (Invitrogen). Samples were heated at 95°C for 5 minutes and collected by centrifugation before loading.
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bioRxiv - Bioengineering 2021Quote: ... the cells were incubated with 2 μM EthD-1 and 4 μM Calcein AM solutions of cell viability/cytotoxicity kit (Invitrogen) for 20 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen) in solution with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... 2X SSC) for 5 min and counter stained with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole; Life Technologies). Coverslips were mounted in imaging buffer (3.7 μg/μl glucose oxidase and 1U catalase in equilibration buffer ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were washed three times with PBS and nuclei counterstained with 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI) (#D3571, Invitrogen) in PBS for 30 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies were applied in incubation buffer (1:500 in PBS/BSA) with 4‘,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... organoids were incubated with 2 mM Calcein-AM and 4 mM Ethidium homodimer-1 (LIVE/DEAD assay kit, Molecular Probes) for up to 6h ...
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bioRxiv - Immunology 2022Quote: ... Doublets and dead cells were excluded based on forward scatter (FSC) and side scatter (SSC) and 4′,6-diamidino-2-phenylindole staining (DAPI, 1 µg/ml; ThermoFisher). All depicted flow cytometry plots were pre-gated on non-debris (by FSC and SSC) ...