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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for 2 Chloro 1 1 trifluoromethyl 1 3 4 9 tetrahydro 2H beta carbolin 2 yl ethan 1 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The monolayers were then overlaid with 1 to 2 ml of a mixture of 1 part 0.6% agarose (Invitrogen) and 1 part complete Eagle Minimal Essential Medium (VWR ...
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bioRxiv - Microbiology 2021Quote: ... 800 µl of isolation medium (DMEM, supplemented with 2% FBS, 1% penicillin-streptomycin and 1% amphotericin B, ThermoFisher Scientific) was added to each well ...
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bioRxiv - Physiology 2020Quote: ... the medium was switched to DMEM/F12 (1:1) plus 2% New Zealand fetal bovine serum (Thermo Fisher Scientific), hormones ...
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bioRxiv - Immunology 2021Quote: ... at 1:0.5:2 molar ratio using Lipofectamine™ 2000 (0.6 ul per 1 ug DNA; Invitrogen™, 11668019); growth medium was refreshed 16 h after transfection ...
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kinesin recruitment by adapter SKIP on melanosomes is dynamically controlled by LC3B phosphorylationbioRxiv - Cell Biology 2021Quote: ... The tumor was excised and was washed in homogenization buffer (0.25 M sucrose [Merck, CAS 57–50–1], 10 mM HEPES, 1 mM EDTA, 2% antibiotic and antimycotic [ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 h at room temperature and stained for 10 min with 4,6-diamidino-2-phenylindole (DAPI; 1 mg/ml stock solution diluted 1:50,000 in PBS; Thermo Fisher) at room temperature with at least four PBS washes in-between ...
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bioRxiv - Immunology 2022Quote: ... resuspended in 1 mL nuclei wash buffer (2% BSA and 0.2LULul−1 RNase inhibitor (Thermo Fisher, cat. no. AM2694) in 1X PBS ...
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bioRxiv - Biochemistry 2024Quote: ... and p976 were packaged in HEK-293T cells at a ratio of 2:1:1 by Lipofectamine 2000 (Invitrogen). The ratio of liposome to DNA was 2:1 ...
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bioRxiv - Neuroscience 2024Quote: ... pCMVR8.74 and pLVX-EF1α-mitoSRAI-IRES-Puro at a 1:1:2 molar mass ratio using Lipofectamine 3000 (Invitrogen). The next day ...
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bioRxiv - Molecular Biology 2023Quote: ... Inoculated cells were incubated for 1 h at 37°C and then overlaid with a 1:1 mixture of 1.2% Oxoid agar and 2× DMEM (Gibco) with 10% (vol/vol ...
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bioRxiv - Developmental Biology 2023Quote: ... washed 1 x 30 min at room temperature in PBSTE-1% BSA supplemented with 2% phalloidin-AF488 (Invitrogen A12379), washed 4 x 30 min at room temperature in PBSTE-1% BSA and washed 2×30’ at room temperature in PBSTE ...
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bioRxiv - Immunology 2022Quote: ... OT-I T-cells (∼400,000 in 1 mL growth media) were loaded with 1 μM Fura-2 AM (F1221, ThermoFisher) for 40 min RT ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1-2 h at room temperature (1:1000) for viral titration or an anti-GFP antibody (A11122, Invitrogen) for measurement of oxGFP-positive cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... were labeled in loading buffer (HBSS with 1% FBS, 1 mM CaCl2 and 1 mM MgCl2) containing 1 μM Fluo-4 AM (Thermo Fisher) with 0.02% Pluronic F-127 at 37 °C for 30 min with gentle agitation every 10 min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 100 μg mL−1 hygromycin (31282-04-9, ≥98%, Invitrogen). Cells were split 1:5 when confluency reached 70% and discarded after passage 10 ...
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bioRxiv - Microbiology 2022Quote: Cell toxicity due to the compound treatment was determined using the standard colorimetric MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was measured by MTT assay using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide kit (Thermo-Fisher Scientific M6494). For the isobologram assays ...
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bioRxiv - Bioengineering 2024Quote: ... and fresh media was added and incubated for a further 24 hours before performing the viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Thermo Fisher Scientific). The media was replaced with fresh cell culture media containing MTT (0.25 mg/mL ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% penicillin/streptomycin (Pen/Strep) and 0.5 μM beta-mercaptoethanol (Life Technologies). Medium was changed every second day and cells were passaged at 1:3 or 1:5 ratio ...
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bioRxiv - Microbiology 2024Quote: ... sheep polyclonal anti-dopamine beta hydroxylase (DBH, Thermo Fisher Scientific, 1:50); Tuj1 mouse anti-tubulin-β-III (Tuj1 ...
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bioRxiv - Cancer Biology 2024Quote: ... ELISA was performed using IL-1 beta Human ELISA Kit (Invitrogen #KHC0011) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... brains were then incubated in secondary antibodies (diluted in 5% serum in PBT at 4°C for 2–4 days): Alexa 488 anti-Chicken IgY (Invitrogen A11039; 1:400); Atto 647N anti-mouse IgG (Rockland 610-156-121 ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then washed 3 times in PBS and incubated with appropriate fluorochrome secondary antibody diluted in blocking solution for 1-2h (AlexaFluor® 488, AlexaFluor® 568, 1:400, Life Technologies). Finally ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunolabeling with performed with mouse anti-B23 primary antibody (1:300; 2h) then detected with Alexa 488 donkey anti-mouse antibody (Invitrogen; 1:500; 1h), then further fixed with 4% paraformaldehyde in DEPC-PBS ...
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bioRxiv - Immunology 2024Quote: ... at a ratio of 4:3:1 using Lipofectamine 3000 kit (Thermo Fisher Scientific). Viral supernatant was filtered ...
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bioRxiv - Biophysics 2024Quote: ... and 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydro (EDC, ThermoFisher). For BS3 crosslinking ...
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bioRxiv - Genetics 2021Quote: ... containing a 4:1:1 mixture of Lipofectin transfection reagent (ThermoFisher Scientific), water and BAC DNA (~ 15 μg DNA per larva) ...
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bioRxiv - Microbiology 2021Quote: ... The 5’ and 3’ fragments (2 μg of each) were ligated for 1 h at 16°C by T4 DNA ligase (Invitrogen) and purified by phenol-chloroform extraction ...
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bioRxiv - Biophysics 2021Quote: ... slides were rinsed in PBS for 3 x 5 mins and stained with 1 %g/mL 4,6-diamino-2-phenylindole (DAPI - Molecular Probes) for 3 mins in the dark at RT ...
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bioRxiv - Biochemistry 2021Quote: ... 1-O-(6-BODIPY®558/568-aminohexyl)-2-BODIPY®FL C5-sn-glycero-3-phosphocholine (Thermo Fisher Scientific) as described previously [38] ...
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bioRxiv - Immunology 2022Quote: ... The sorted cells were then incubated with Totalseq-C antibodies (1:50 dilution) on ice for 30 min, washed 3 times (by default, top up with 2% FBS (Gibco) in DPBS (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... The next day, the larvae were washed (3×, 1× PBST) and incubated for 30 minutes in 2 μg/mL Hoechst 33342 (Invitrogen) in 1× PBT at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... 20 ng/mL TPO and 40 ng/mL IL-3 from day 1 to day 6 with a media refresh every 2 days (all cytokines from Thermofisher). On day 8 ...
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bioRxiv - Immunology 2022Quote: ... The dissociated cells were washed 3 times with chilled PBS containing 1% heat-inactivated FBS and 2 mM EDTA (Gibco).
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed 3×2 minutes with DPBS before a permeabilization solution (0.1% Triton-X (Fisher Scientific, 9002-93-1) in DPBS ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were then incubated for 1 hour in 2-3 drops per slide of HRP-conjugated streptavidin (Alexa Fluor 594 Tyramide Superboost Kit, Invitrogen), washed 3 times for 10 minutes each in 1x PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Each individual organoid was incubated in 1.5 mL of Trypsin/Accutase (1:1) containing 1 µL/mL of TURBO™ DNase (Thermo Fisher Scientific, AM2238, 2 U/µL) in a gentleMACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... PDCA-1 (ThermoFisher # 53-3179-4), TLR9 (Biolegend #394803) ...
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bioRxiv - Pathology 2023Quote: ... Fluo-4-AM (1 µM; Invitrogen), in the dark for 20 minutes at room temperature in Mg2+-PSS ...
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bioRxiv - Neuroscience 2023Quote: ... (1:300; 4 h, RT, Invitrogen) were used as secondary antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... OCT-4 (701756, Invitrogen, 1:1000), TRA-1-60 Podocalyxin (14-8863-82 ...
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bioRxiv - Biophysics 2022Quote: ... Oocytes were injected with 0.1 to 10 ng cRNA of mASIC1a (volumes between 9 and 50 nL) and incubated for 1–4 days at 18 °C in Leibovitz’s L-15 medium (Gibco), supplemented with 3 mM L-glutamine ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated for 1 h with 2 μM EdU (Thermo Fisher) and fixed in 70% ethanol ...
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bioRxiv - Cell Biology 2020Quote: ... 1-2 μg of Plasmid DNA along with transfection agents Lipofectamine (Invitrogen) or Plyetheleneimoine ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... 2% vol/vol FBS and 1% vol/vol antibiotic-antimycotic (#15240062, Gibco, Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... 2 µl 5 M NaCl and 1 µl GlycoBlue co-precipitant (Invitrogen). Samples were vortexed and incubated at room temperature for 15 min ...
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bioRxiv - Bioengineering 2021Quote: ... media was replaced and supplemented with 2 μg mL−1 puromycin (Gibco).
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bioRxiv - Neuroscience 2021Quote: ... Cortices were homogenized in 1-2 ml of TRIzol (Thermo Fisher Scientific). RNA was extracted following the manufacturer’s protocol and purified using the mirVana miRNA Isolation Kit (Ambion/Life Technologies).
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bioRxiv - Microbiology 2021Quote: ... Plasmids were transfected in a 1:2 ratio with Lipofectamine 2000 (ThermoFisher).