Labshake search
Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for Zika Virus NS1 Proteins Uganda Suriname Strains Duo Pack since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Proteins were quantified using BSA Protein Assay Kit (ThermoFisher). Equal amounts of protein were boiled with 6X loading buffer (375mM Tris HCL ...
-
bioRxiv - Microbiology 2020Quote: ... Protein concentration was measured using BCA Protein Assay (ThermoFisher). For anti-NP immunoprecipitation ...
-
bioRxiv - Microbiology 2020Quote: ... Protein concentrations were determined with Bradford protein assay (Thermofisher), following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... cas9 protein (Fisher Scientific A36498 Truecut Cas9 Protein v2) and gRNA were co-electroporated into G1 ES cells derived from an F1 hybrid blastocyst of 129S6 x C57BL/6J ...
-
Allelic diversity uncovers protein domains contributing to the emergence of antimicrobial resistancebioRxiv - Microbiology 2022Quote: ... alongside protein standards (SeeBlue Pre-stained Protein Standard, Invitrogen). Gels were run at room temperature for 150 min at 80 V in running buffer ...
-
bioRxiv - Plant Biology 2022Quote: ... the magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4°C for 2 hours ...
-
bioRxiv - Plant Biology 2023Quote: ... the magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and mixture was rotated at 4°C for 2 hours ...
-
bioRxiv - Neuroscience 2023Quote: ... cas9 protein (Fisher Scientific A36499 TRUECUT CAS9 PROTEIN V2) and the gRNA with concentrations of 10 ug ...
-
bioRxiv - Biochemistry 2023Quote: ... protein quantified by BCA protein assay ( Thermo scientific #23250), and 30 μg of the proteins were loaded on 4-12% Criterion TGX Stain-Free precast polyacrylamide gels (BIO-RAD ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein concentration was determined by Qubit protein assay (Invitrogen) in a Qubit 4 fluorometer (Invitrogen).
-
bioRxiv - Cell Biology 2023Quote: ... 200 μL of Protein A (Dynabeads Protein A, Invitrogen) beads were washed twice in coIP buffer containing PIs ...
-
bioRxiv - Cancer Biology 2024Quote: ... Proteins were quantified using Coomassie Protein Assay Reagent (ThermoFisher). Protein samples (30 μg ...
-
bioRxiv - Microbiology 2020Quote: ... five-fold serial dilutions of each virus stocks were prepared in a separate 96-well plate in Opti-MEM (Gibco). Medium in the 96-well plate was removed and the cells were washed with 100 µL of warm PBS ...
-
bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription and quantitative PCR (RT-qPCR) was performed using 0.25 µl of lysate and Taqman Fast Virus 1-step Master Mix (Applied Biosystems) on a CFX384 thermal cycler (Bio-Rad) ...
-
bioRxiv - Cell Biology 2020Quote: ... the plate was checked for confluence and the cells were infected with serial dilution of virus in OptiPro serum free media (Thermofisher) with 1% L-Glutamin (Life Technologies ...
-
bioRxiv - Neuroscience 2021Quote: ... 1 μl of RNAse-Out and 1 μl of the Moloney Murine Leukemia Virus reverse transcriptase enzyme (MML-V, Invitrogen). Quantitative PCR was carried out in a total of 15μl consisting of 3μl cDNA ...
-
bioRxiv - Plant Biology 2020Quote: ... NAA50 was cloned into the cauliflower mosaic virus 35S promoter vector pEarleyGate100 (Earley et al. 2006) using a modified multisite Gateway recombination cloning system (Invitrogen) as described in (Qi et al ...
-
bioRxiv - Developmental Biology 2019Quote: Pure cultures of 10 × 104 prostate stromal cells seeded on twelve well plates were transduced using Cytotune 2.0 Sendai Virus reprogramming vectors (KOS, c-Myc and Klf4, Life Technologies) at a multiplicity of infection of 5-5-3 (KOS MOI=5 ...
-
bioRxiv - Microbiology 2019Quote: ... 0.35μg of each of 10 reovirus genes and 0.8μg C3P3 (T7 RNA Polymerase linked with (G4S)4 to NP8686 African Swine Fever Virus capping enzyme) were diluted in 50μl Opti-MEM (31985070, ThermoFisher Scientific). 11μl TransIT®-LT1 (MIR2300 ...
-
bioRxiv - Cell Biology 2019Quote: ... and HY03 were reprogrammed using the Sendai virus and the CytoTune®-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific), and display all the PSC features ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were washed three times with PBS to remove residual input virus and overlaid with DMEM with glutamine (Life Technologies) with 0.7% IgG-free BSA (Sigma) ...
-
bioRxiv - Bioengineering 2021Quote: ... Untransduced cells and cells treated with the serially diluted virus were both cultured in the presence of 2 µg/ml puromycin (Invitrogen). The lentiviral titer was quantified by counting the number of living cells at the time when all of the untransduced cells died as previously described26.
-
bioRxiv - Biochemistry 2020Quote: ... The plasmid was linearized with XbaI and in vitro transcribed into the genomic RNA of DENV-Luc virus using the T7 Megascript Kit (Ambion) in the presence of m7G(5’)ppp(5’)G RNA Cap Structure Analog (NEB) ...
-
bioRxiv - Biochemistry 2021Quote: ... The virus particles were then harvested 24 to 48 hours later by passing through a 0.45 µm syringe filter (Thermo Scientific). Viruses were mixed with an equal volume of fresh media supplemented with 10% FBS and polybrene was added at a final concentration of 5 µg/mL to increase infection efficiency ...
-
bioRxiv - Neuroscience 2021Quote: ... Cell reprogramming was performed using the Sendai virus system with SOX2/OCT4/KLF4/MYC (CytoTune-iPS Reprogramming Kit; Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... by the acid guanidinium/phenol/chloroform method using ISOGEN (Nippon Gene) was converted to cDNA using Moloney murine leukemia virus reverse transcription (Invitrogen) and oligo-dT primers ...
-
bioRxiv - Bioengineering 2021Quote: ... a pure DI244 seed virus at MODIP 1E–3 and 20 U/mL trypsin (5000 U/mL in PBS; 27250018, Gibco) were added ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Copies of SARS-CoV-2 E gene were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
-
bioRxiv - Plant Biology 2021Quote: ... ROMT2 and ROMT3 were transferred into the binary destination vector pB2GW7 including Cauliflower mosaic virus (CaMV) 35S promoter through Gateway LR clonase recombination (Invitrogen). Together with ROMT1 and STS48 constructs (Schmidlin et al. ...
-
bioRxiv - Microbiology 2021Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...
-
bioRxiv - Microbiology 2021Quote: Nucleic acids were extracted from purified virus samples either using the PureLink Viral RNA/DNA Mini Kit (Thermo Fisher Scientific) or by phenol/ether method combined with ethanol precipitation ...
-
bioRxiv - Microbiology 2021Quote: ... VeroE6 cells were used for live virus titration of biological samples and were maintained in DMEM (#11965092, Thermo Scientific, USA) with 10% FBS.
-
bioRxiv - Microbiology 2021Quote: ... Five μl RNA (150 µl elution volume) was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed using 100ng total RNA compared to an RNA standard curve using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on a Quant Studio 3 (Applied Biosystems).
-
bioRxiv - Microbiology 2020Quote: ... Viral RNA was quantified by RT-qPCR using the TaqMan(tm) Fast Virus 1-Step Master Mix (Thermo Fisher Scientific). Primers and probes were based on (21 ...
-
bioRxiv - Cell Biology 2021Quote: ... Viral RNA was quantified by RT-qPCR using the TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific). Primers and probes were used as described previously (45) ...
-
bioRxiv - Cell Biology 2022Quote: ... 4T1 cells were transduced with cell-free virus-containing supernatants for 48 hours and selected with 100 µg/mL of neomycin (Gibco) for 96 hours.
-
bioRxiv - Immunology 2022Quote: ... were pulsed for 1 hr with 10μg/ml influenza virus NP(366-374) peptide and were subsequently labeled for 10 min at 37°C with 5 μM CFSE (Invitrogen) (CFSEhigh ...
-
bioRxiv - Immunology 2022Quote: ... The extracted RNA was then added to TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, Waltham, MA) containing primers and a fluorescently labeled hydrolysis probe specific for mRNA from the nucleocapsid gene of SARS-CoV-2 (forward primer 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
-
bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA was detected with the CDC assay kit (IDT) and the TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on the QuantStudio3 System (Invitrogen).
-
bioRxiv - Microbiology 2022Quote: ... The nCoV_IP2 primer-probe set was used in combination with TaqMan fast virus 1-step master mix (Thermo Fisher Scientific) to detect viral RNA ...
-
bioRxiv - Cell Biology 2022Quote: ... Assays were performed in 24-well plates coated with Geltrex LDEV (lactose dehydrogenase elevating virus)-Free Reduced Growth Factor Basement Membrane Matrix (ThermoFisher) at 75 µl/cm2 according to the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2020Quote: ... The resuspended virus was purified using a 30% (wt/vol) sucrose cushion at 100,000 × g (Sorvall Discovery 90SE, Thermo Fisher) for two- and-a-half hours at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... 500 ng of RNA was reversely transcribed using random primers (Eurofins) and Moloney murine leukaemia virus reverse transcriptase (M-MLV, Invitrogen) according to manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2021Quote: Heathy human donor PBMCs or skin fibroblasts were reprogrammed into iPSCs using the integration-free based Sendai virus (Cytotune 2.0 kit from Life Technologies). Low passage primary fibroblasts were used to increase reprogramming efficiency ...
-
bioRxiv - Microbiology 2022Quote: ... The presence of engineered mutations was confirmed by Sanger sequencing following extraction of RNA from virus stocks with TRIzol (Invitrogen) and cDNA amplification using a OneStep RT-PCR Kit (Qiagen ...
-
bioRxiv - Microbiology 2022Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...
-
bioRxiv - Microbiology 2019Quote: vRNA was extracted from virus supernatant containing the same amount of PFU using PureLink Viral RNA/DNA Mini Kit (Invitrogen). vRNA was reverse transcribed with Uni12/13 specific primers using Superscript IV First-Strand Synthesis System (Invitrogen ...
-
bioRxiv - Neuroscience 2019Quote: ... filled with the infusate (virus particles suspended in a solution of phosphate-buffered saline (PBS) and Oregon Green 488 (Invitrogen)) into the centers of the craniotomies ...
-
bioRxiv - Molecular Biology 2019Quote: iPSCs were generated by transducing control and FXS patient fibroblasts with Sendai virus from the Cytotune 2.0 Reprogramming Kit (Life Technologies) per the manufacturer’s protocol ...