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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for TNF RII Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... PfP2 sample was purified after binding with Hi-Bind Ni-NTA beads (Invitrogen) for 4h followed by elution of the sample with 20mM Tris-HCl (pH 7.4) ...
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bioRxiv - Developmental Biology 2020Quote: ... each experiment sample was separated on a Hi-pH column (Thermo Fisher Scientific) according to the vendor’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... The His-tagged proteins were purified on Ni-NTA resin (Thermo Scientific #88222) and washed into a buffer containing 50 mM Tris (pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: MBP-His-TEV-Epe1 was cloned into a pFastBac vector (Thermo Fisher Scientific) and used for Bacmid generation ...
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bioRxiv - Microbiology 2021Quote: ... The products were cloned into the pcDNA3.1D/V5-His’s Topo expression vector (Invitrogen) and NL4.3 ...
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bioRxiv - Biochemistry 2020Quote: ... 6x-His Tag Antibody (clone HIS.H8) (1:1000, # MA1-21315) was from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and into pVL847 (His-MBP-tag overexpression vector) by Gateway cloning method (Invitrogen). ΔnahA mutant of Bacillus subtilis was constructed by CRISPR/Cas9 editing method (Ref) ...
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bioRxiv - Molecular Biology 2021Quote: Western blot analysis was performed using an anti-His antibody (Invitrogen, LOT 1902132) to confirm the identity of splicing and cleavage products ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Thermo Fisher Scientific) and 1% Pen-Strep (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... plates were coated with 1 μg/mL 6x-His tag polyclonal antibody (Invitrogen) in 1x PBS for 6 hours at ambient temperature and blocked overnight with 0.5% NCS/5% Goat Serum/5% Milk/0.2% PBS-T ...
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bioRxiv - Immunology 2021Quote: ... or left unstimulated in 10% HI-FBS RPMI-1640 (ThermoFisher Scientific cat. 11875085) containing Brefeldin A (eBioscience cat ...
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bioRxiv - Microbiology 2021Quote: ... gingivalis were stained with 15 mg/l hexidium iodide (HI; Thermo Fisher Scientific) and 4 mg/l 5-(and-6)-carboxyfluorescein and succinimidyl ester (FITC ...
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bioRxiv - Cell Biology 2021Quote: ... before being incubated with the primary antibody (6x-His Tag Monoclonal Antibody, Invitrogen) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... and His-tagged mCherry (strain He/80/FR) were synthesized by Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged protein was purified using HisPur Cobalt Resin (Thermo Fisher Scientific) in 20 mM Hepes (pH 7.3) ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (hi-FBS, Gibco, 16140-071), 100U/mL Penicillin/100ug/mL Streptomycin (pen/strep ...
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bioRxiv - Plant Biology 2022Quote: ... the secondary antibody used was anti-6x-His tag monoclonal (Invitrogen, # MA1-21315) with 1:100 dilutions ...
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was then purified using Ni-NTA His affinity agarose (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... 6x-His Epitope Tag Monoclonal Antibody (#MA1-21315, Thermofisher Scientific; dilution 1:500) was used for His-tag staining of the TRPA1 channel in fixed cells for 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... each experiment sample was separated on a Hi-pH column (Thermo Fisher Scientific) according to the vendor’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... recombinant proteins were detected using primary anti-His antibody (ThermoFisher, Cat. # PA1-983B) and an anti-RNAP antibody (BioLegend ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 μl of Dynabeads™ His-Tag Isolation and Pulldown beads (ThermoFisher 10104D) for each CFPS reaction were equilibrated twice in equal volumes of wash buffer before addition to the purified reactions to remove the Ulp-protease and incubated with spinning at 4 °C for at least 30 minutes ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then incubated with an anti-His antibody with HRP conjugate (Invitrogen) in 1% BSA at 4°C overnight with gentle rocking ...
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bioRxiv - Cancer Biology 2023Quote: ... neutralized by adding RPMI-1640 with 10% heat inactivated FBS (HI FBS, Gibco), and spun down at 200 x g for 10 minutes to pellet the cells ...
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bioRxiv - Plant Biology 2023Quote: ... Immunoblotting was performed using anti-His antibody (Thermo Fisher, Cat. No. MA1-21315) at a dilution of 1:10,000 ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% (v/v) heat-inactivated foetal calf serum (hi-FCS) (Gibco) and 1% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Western blots were carried out probed with anti-His mAb (1:1000, Invitrogen) followed by anti-mice IgG-HRP (1:20000 ...
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bioRxiv - Cancer Biology 2022Quote: ... medium containing 10% (v/v) heat inactivated fetal calf serum (HI-FCS; Gibco), 100 U/mL Penicillin (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... The His-tagged protein was purified using HisPur Cobalt Resin (Thermo Fisher Scientific) in 20 mM Hepes (pH 7.3) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10 % v/v heat-inactivated fetal calf serum (HI-FCS, Gibco), 1 mM non-essential amino acids (NEA ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 1% (v/v) HI-FBS and N-2 supplement (Gibco™). ATM knockout (KO ...
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bioRxiv - Genetics 2023Quote: ... Libraries were mixed with Novex 5X Hi-Density TBE Sample Loading Dye (Thermofisher), then loaded into a 6% TBE acrylamide gel with the Quick-Load pBR322 DNA-MspI Digest ladder and run for 65 minutes at 120 V ...
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bioRxiv - Biochemistry 2023Quote: ... Successful binding was analyzed using anti-His Tag antibody (PCRD, Invitrogen #MA1-135) or anti-Myc Tag (DCRD ...
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bioRxiv - Molecular Biology 2023Quote: ... MBP-His-TEV-Epe1 was cloned into a pFastBac vector (Thermo Fisher Scientific) and used for Bacmid generation ...
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bioRxiv - Cell Biology 2024Quote: ... excluding the HI-FBS and supplementing with 0.1% BSA (Life Technologies 15260-037). CHIM was used for the experiments in Figures 4 ...
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bioRxiv - Synthetic Biology 2024Quote: ... then diluted at 1:100 in Hi-Def Azure Media (Fisher Scientific # 3H5000) supplemented with 1% Glucose and various concentrations of peptide in 100 µl in a 96well plate ...
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bioRxiv - Plant Biology 2024Quote: ... synthesized GUN1-FL protein was purified using His-Tag Dynabeads™ (Invitrogen; 10103D) and confirmed by Western blotting using His-tag antibody (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were placed in HI buffer containing 1:1,000 CellMask Deep Red (Invitrogen) for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Bio-His-FIKK13 was incubated with different quantities of magnetic streptavidin beads (Invitrogen) for 30 min ...
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bioRxiv - Immunology 2021Quote: ... 17-7311-81) and TNF-α (eBioscience, 12-7321-81)) were stained before fixation/permeabi-lization (Intracellular Fixation & Permeabilization Buffer Set, ThermoFisher).
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bioRxiv - Immunology 2020Quote: ... IL-23 and tumor necrosis factor alpha (TNF-α) were quantified in lung tissue lysates and BAL using commercial ELISA kits (Invitrogen, San Diego ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... IL-6 and TNF-α in RAW264.7 cells culture supernatants were analyzed using ELISA kits (No. EMIL6RA, 88-7324-22, bms6002; Thermo Scientific) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... ELISAs were conducted following the manufacturers’ protocols, with the IL-1b and IFN-y (ELISAKIT.com, EK-0033 and EK-0002, respectively) and TNF-a (ThermoFisher Scientific KMC4022). Samples were diluted 1 in 5 and 1 in 10 for IL-1b ...
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bioRxiv - Immunology 2020Quote: ... Capture antibodies were anti-IL-6 (14-7061-85, eBioscience), anti-TNF-α (14-7423-85, eBioscience) and anti-IL-1β (14-7012-81, Invitrogen). Detection antibodies conjugated with biotin were anti-mouse-IL-6 (554402 ...
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bioRxiv - Cell Biology 2022Quote: ... established mature DB-VOs at day 15 from diabetic iPSCs were started to culture in high glucose concentration (33mM, adapted from our previous publication (Yang et al., 2020)) plus 1 ng/ml human TNF (Invitrogen, PHC3011) and 1 ng/ml IL-6 (Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... Samples that were determined to be below the detection limit where quantified using <0.3 pg/mL sensitivity porcine TNF-α ELISA kit (Invitrogen, USA).
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bioRxiv - Bioengineering 2023Quote: ... MSCs were treated with either MSC-GM or MSC-GM + 50 ng/mL IFN-γ/TNF-α (Gibco, Sino Biological, respectively) for 24 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Human PAR4 containing an N-terminal V5-epitope were stably expressed using the HEK293 Flp-In T-Rex cells according to the manufacturer’s protocol (Invitrogen). The concentration of tetracycline was titrated (700 ng/mL ...
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bioRxiv - Molecular Biology 2021Quote: For siRNA transfection experiments: HEK293 cells were grown in 6-well plates and RNAimax transfection reagent (Invitrogen #13778-150) was used following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells were transfected with either NSUN6 wild-type and the miCLIP-mutant construct using Lipofectamine 2000 (Life Technologies) and harvested 24 hours later.