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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for TNF RII Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... These fragments were inserted into the pIZ-V5/His vector (Invitrogen) using the In-FusionHD Cloning Kit (Takara) ...
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bioRxiv - Molecular Biology 2022Quote: ... ceylanicum were subcloned into the pMT/BiP/V5-His vector (ThermoFisher) in frame with the BiP signal sequence and the C-terminal V5 and six-histidine tags ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were directly incubated with DyLight680-conjugated anti-His antibody (Thermofisher), washed ...
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bioRxiv - Microbiology 2020Quote: ... The His-MspGH25 cloned into pGAPZαA vector (Invitrogen, Carlsbad, CA, USA) under the control of a constitutive promotor with an α-factor signal peptide for secretion ...
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bioRxiv - Zoology 2019Quote: ... Blots were probed with rabbit anti-His antibody (Thermo Fisher Scientific) at 1 ...
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bioRxiv - Immunology 2020Quote: ... Recombinant His-HMGB1 was produced in 293-freestyle cells (ThermoFisher Scientific). Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant CEACAM-His proteins were expressed in Expi293F cells (Life Technologies) and purified by affinity chromatography (ÄKTA Pure ...
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bioRxiv - Microbiology 2021Quote: ... using an Ion PGM Hi-Q Sequencing Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions to ensure a high degree of sensitivity required and the deal with the high complexity of our samples under investigation.
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bioRxiv - Biophysics 2019Quote: ... 1 nM LanthaScreen Elite Tb-anti-HIS Antibody (Thermo Fisher Scientific), and 5 nM Fluormone Pan-PPAR Green (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: ... and His-BMAL1 FL were co-infected into monolayer HighFive (Invitrogen) insect cells for protein expression ...
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bioRxiv - Evolutionary Biology 2021Quote: ... suspended in Hi-Di formamide (Applied Biosystems, Foster City, CA, USA) and run on an ABI 3730 at the University of Georgia ...
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bioRxiv - Immunology 2020Quote: ... and 10% heat-inactivated fetal bovine serum (HI-FBS, Invitrogen, 12484028). DMEM supplemented media consisted of 2 mM GlutaMAX ...
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bioRxiv - Immunology 2021Quote: ... washed with 1% HI-FBS RPMI-1640 (ThermoFisher Scientific cat. 11875085) and red blood cells were lysed using 1x RBC Lysis Buffer (eBioscience ...
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bioRxiv - Immunology 2021Quote: ... 1◻nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher #PV5895), and 400 nM FITC-labeled PGC1α peptide (residues 137–155 ...
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bioRxiv - Molecular Biology 2020Quote: ... The empty coding vector pcDNA3.1/MYC-His was obtained from Invitrogen and pCMV-β-Galactosidase was obtained from Clontech/Takara Bio.
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bioRxiv - Molecular Biology 2022Quote: ... the following antibodies were used: 6x-His Tag monoclonal antibody (ThermoFisher, cat ...
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bioRxiv - Microbiology 2023Quote: ... 1-2 μl of primary anti-His (PA1-983B, Thermo Scientific) or anti-alpha RNAP (4RA2 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µL of His-Tag magnetic beads (Dynabeads™ Invitrogen #10103D) was added to each tube ...
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bioRxiv - Microbiology 2023Quote: ... and inserted into similarly digested pcDNA4-To/myc-His B (Invitrogen), resulting in pcDNA4-L3HYPDH ...
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bioRxiv - Microbiology 2023Quote: ... 10% heat-inactivated fetal bovine serum (HI-FBS; Hyclone and Gibco), and 100 U/mL penicillin/streptomycin (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were taken for immunoblotting using anti His-tag antibody (ThermoFisher). Crosslinking and control reactions intended for scramblase activity assays were stopped with 1 M Tris pH 7 to a final concentration of 10 mM ...
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bioRxiv - Biochemistry 2023Quote: ... All media was supplemented with 10% HI FBS (Gibco cat # 10438026).
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% (v/v) heat-inactivated FBS (HI-FBS; Gibco). Immortalised adult human C20 microglial cells were kindly provided by Dr David Alvarez Carbonell ...
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bioRxiv - Biochemistry 2023Quote: ... Hi Five cells grown in Sf-900 II SFM media (Gibco) at a density of 2.0 × 106 cells/mL were infected with baculovirus at 1.5% v/v ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with Novex Hi-Density TBE Sample buffer (ThermoFisher Scientific, LC6678) and analysed on 10% Novex TBE gels (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... His-HcpGA1 was purified using the ProBond Purification System (Life Technologies) and the eluted protein was dialyzed against PBS and used as immunogen for antiserum generation.
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% heat-inactivated fetal calf serum (HI-FCS, Gibco) and 1% of Penstrep (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 10% fetal bovine serum (Gibco FBS HI, Cat# A5256701) and 1% penicillin-streptomycin (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... followed by Western blot analysis using anti-His antibody (Invitrogen, USA).
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bioRxiv - Microbiology 2023Quote: ... 2 µL of Novex Hi-Density TBE 5X Sample Buffer (Invitrogen) was mixed with 8 µL of the completed EMSA reaction and loaded on an 8% acrylamide gel ...
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bioRxiv - Biophysics 2023Quote: ... To cut the His-tag HRV 3C protease (Thermo-Fisher Scientific) was added to a molar ratio of 1:800 (enzyme ...
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bioRxiv - Bioengineering 2019Quote: ... The resulting VH-(G4S)3-VL ScFv fragment was further fused at the N-terminus of the murine TNF gene through a S4G-linker and the final construct VH-(G4S)3-VL-(S4G)3-TNF was then cloned into the mammalian expression vector pcDNA3.1 (+) vector (Invitrogen). A VH-(G4S)3-VL ScFv fragment specific for hen egg lysozyme (KSF)(34) ...
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bioRxiv - Molecular Biology 2023Quote: ... TNF-α and IL-6 in cell culture supernatants were assayed using standard ELISA kits (Invitrogen or R&D) in accordance with the protocol set out by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... and TNF-α (cat#BMS223-4) were measured in duplicate using human ELISA kits following the manufacturer’s instructions (ThermoFisher) and SpectraMax M3 (Molecular devices).
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bioRxiv - Cell Biology 2024Quote: ... this is equivalent to total of 190,000 cells) in the morning and stimulated overnight with 10 ng/mL TNF (PHC3015, Invitrogen). The RBC control was prepared by culturing a 2% haematocrit suspension in RPMI medium overnight ...
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bioRxiv - Microbiology 2020Quote: ... HEK293 cells were transfected with pCMV-AT1R-IRES-Puro gene using Lipofectamine 3000 reagent (Thermo Fisher Scientifics). Approximately 24 hours posttransfection ...
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bioRxiv - Immunology 2021Quote: HEK293 and RAW cells were transfected using Lipofectamine 3000 or Lipofectamine LTX Transfection Reagent (Life Technologies, # L3000015) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: Total RNA from HEK293 cells and human brain prefrontal cortex were isolated with the TRIzol reagent (Ambion) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2020Quote: ... not commercially obtained but can be provided on request) (42) and HEK293-FT (R70007, Thermo Fisher Scientific) cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Physiology 2021Quote: ... The expression vector was co-transfected with pOG44 into Flp-In T-REx HEK293 cells (Life Technologies), in accordance with the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Cells were cultured in DMEM (HEK293, Neuro-2a) or RPMI 1640 (SH-SY5Y) with 10% FBS (Gibco). Cells grew on petri dishes to a density of 1×105/cm2 ...
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bioRxiv - Biochemistry 2020Quote: Immunoprecipitation from HEK293 cell lysates was conducted using Dynabeads protein G magnetic beads (Invitrogen, Thermo Fisher Scientific) according to manufacturer’s protocol with a following modification ...
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bioRxiv - Biochemistry 2020Quote: Immunoprecipitation from HEK293 cell lysates was conducted using Dynabeads protein G magnetic beads (Invitrogen, Thermo Fisher Scientific) according to manufacturer’s protocol with a following modification ...
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bioRxiv - Bioengineering 2022Quote: ... hACE-2-expressing HEK293 cells were seeded on a Nunc Lab-Tek Chamber Slide system (Thermo Fisher) and cultured overnight ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293-PSMB2-HB cells were transfected with 5 μg of the cloned plasmid using Lipofectamine 3000 (Invitrogen). After 48 h incubation ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 Jump-In and Flp-In TREx cells that allow doxycycline-dependent transgene expression were from Invitrogen. Cell lines were checked for mycoplasma infection by PCR or ELISA ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293-based EGFP reporter and Hela cells were maintained in Dulbecco’s Modified Eagle’s medium (DMEM, ThermoFisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... growth media containing DMEM (HEK293) or DMEM/F12 (CHO) (both high glucose, pyruvate, Thermo Fisher Scientific (TFS)) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293S GnTI- cells were grown at 37°C with 8% carbon dioxide in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... Transfected HEK293 cells were solubilized in 0.1-1% (w/v) n-dodecyl-β-D-maltoside (ThermoFisher Scientific), 1 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich) ...