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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for Recombinant Human BTLA Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... containing 10% HI FBS (Life Technologies) and 35 µg/ml Ascorbic acid (Sigma) ...
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bioRxiv - Immunology 2021Quote: 6x His tag monoclonal antibody (Invitrogen) was coated at 2 μg/mL in PBS onto half-area 96-well high binding plates (Corning ...
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bioRxiv - Immunology 2021Quote: 6x His tag monoclonal antibody (Invitrogen) or streptavidin (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2022Quote: ... + 10% HI-Horse Serum (Gibco, 26050088) + 0.8% Pen/Strep (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... (hi-FCS, Gibco, Life Technologies, UK), were incubated (37°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... (hi-FCS, Gibco, Life Technologies, UK), were incubated (37°C ...
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bioRxiv - Microbiology 2021Quote: Plasmid based on pBAD/His (Invitrogen) or pHT01 (MoBiTech ...
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bioRxiv - Microbiology 2020Quote: ... the pIB/V5-His vector (Invitrogen) and insert egfp fragment were amplified by PCR ...
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bioRxiv - Biochemistry 2020Quote: ... Anti‐His tag monoclonal antibody (Invitrogen) was used in western blot analysis.
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bioRxiv - Biochemistry 2021Quote: Modified pBAD/His B vector (Invitrogen) containing native dnrK and tamK31,39 were used as templates for chimeragenesis ...
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bioRxiv - Biochemistry 2021Quote: ... His (1:1000, Invitrogen, mouse monoclonal), Flag M2(1:1000 ...
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bioRxiv - Immunology 2022Quote: ... and Hi-5 cells cells (Invitrogen), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... + 10% HI-FBS (Thermo Fisher, 16140071) prior to adding indicated concentration of human IFN-alpha 2b (pbl Assay Science ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant RNase H (Invitrogen) was later added to the reverse transcribed samples and incubated for 20 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Recombinant B18R (Life Technologies) was used at 1ug/ml ...
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bioRxiv - Cell Biology 2020Quote: ... both cell lines were lysed and biotinylated proteins were isolated using Dynabeads™ MyOne™ Streptavidin C1 (65002, Thermo Fisher Scientific) following the protocol described elsewhere (Roux et al ...
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bioRxiv - Immunology 2021Quote: ... 1 µg of biotinylated S1 protein (Genscripts) was used to saturate the binding sites on 1 µl of FluoroSphere neutravidin beads (Thermo Fisher) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: Cleared lysates containing biotinylated proteins in modified RIPA buffer were incubated with 30 µL of pre-washed streptavidin-coated magnetic beads (Thermo Fisher) for 3 hours at 4°C whilst rotating ...
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bioRxiv - Molecular Biology 2020Quote: ... SARS-CoV-2 S trimer protein was biotinylated using the EZ-Link(tm) Sulfo-NHS-LC-LC-Biotin kit (Thermo Fisher) and then purified using Zeba(tm ...
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bioRxiv - Neuroscience 2022Quote: ... 40 μl of streptavidin magnetic beads were incubated with 4 μg of anti-CD171 (L1CAM, neural adhesion protein) biotinylated antibody (clone 5G3, #13-1719-82, Thermo Fisher) for three hours on ice ...
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bioRxiv - Molecular Biology 2020Quote: The lysate and purified proteins were incubated with 3 µg of biotinylated RNA probe coupled to 20 µl Dynabeads MyOne Streptavidin C1 (Thermo Fisher) in 600 µl lysis buffer at 4°C for 1 hour rotating head-over-tail ...
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bioRxiv - Neuroscience 2021Quote: ... using 100 μg custom monoclonal anti-GFP antibodies (50 μg of clone 19C8 with 50 μg of clone 19F7) bound to biotinylated Protein L (Pierce, Thermo Fisher) coated streptavidin-coated magnetic beads (Thermo Fisher) ...
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bioRxiv - Biochemistry 2020Quote: ... clarified supernatants were incubated with 30μl high-capacity neutravidin agarose beads overnight to capture biotinylated proteins (Thermo Scientific, catalogue number 29204). Beads were then washed 3x with ice-cold RIPA buffer and eluted with 2x SDS sample buffer with excess biotin at 95°C for 15 mins ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated PR8-HA and S proteins were fluorescently labelled by the sequential addition of streptavidin-conjugated to phycoerythrin (PE) (Thermo Fisher) prior to use.
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bioRxiv - Biochemistry 2022Quote: Purified protein at a concentration of 1 mg/ml was chemically biotinylated using EZ link NHS-PEG4-Biotin (Thermo Scientific, A39259) at a 10-30 times molar excess ...
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bioRxiv - Neuroscience 2023Quote: ... Cell surface proteins were biotinylated using the EZ-Link™ Sulfo-NHS-SS-Biotin reagent (Cat No. 21331, Thermo Fisher Scientific). Cells were treated in three experimental conditions (VLIUS ...
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bioRxiv - Cancer Biology 2023Quote: ... CuAAC reaction was performed as previously described23 and biotinylated proteins were enriched using Pierce™ streptavidin agarose beads (Cat. # 20353, ThermoFisher).
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bioRxiv - Systems Biology 2023Quote: ... and samples were normalized accordingly before capture of biotinylated proteins on Pierce™ Streptavidin UltraLink™ Resin (80 μl; ThermoFisher Scientific), washing ...
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bioRxiv - Cell Biology 2023Quote: ... Total biotinylated proteins in samples were detected with Alexa Fluor™ 790 Streptavidin Conjugate (1:20 000, S11378, Thermo Fisher Scientific). Band densities were quantified using Image Studio quantification software (LI-COR Biosciences).
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bioRxiv - Cell Biology 2023Quote: ... Biotinylation and purification of biotinylated proteins were done following the Pierce™ Cell Surface Biotinylation and Isolation Kit’s (A44390, Thermo Scientific) protocol ...
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bioRxiv - Microbiology 2023Quote: ... 0.4pmol of biotinylated SINV RNA was incubated with normalized amounts of protein lysates and RNA binding buffer containing RNaseOUT (Thermo Fisher), heparin (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... The monomer form of the antigens was biotinylated and the subsequent tetramerization of these proteins was done with FITC-labeled avidin (Invitrogen, A821), in brief ...
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bioRxiv - Physiology 2024Quote: ... Biotinylated proteins were precipitated from the remaining lysate by overnight incubation at 4°C with streptavidin agarose resin (Thermo Scientific, #20353) and recovered in 4x loading sample buffer (0.2M Tris-HCl pH 6.8 ...
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bioRxiv - Neuroscience 2019Quote: ... All proteins were expressed in Human Embryonic Kidney (HEK) 293 Freestyle cells (Invitrogen) in suspension culture using serum-free media ...
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bioRxiv - Immunology 2023Quote: ... RBD proteins were expressed in-house in Expi293F human cells (Thermo Fisher Scientific) by transfection of the cells with purified DNA and polyethylenimine (PEI) ...
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bioRxiv - Biochemistry 2023Quote: Proteins from human cell lines were extracted in IP lysis buffer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Biotinylated double-stranded were synthesized probes (ThermoFisher) with the indicated sequence.
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bioRxiv - Bioengineering 2020Quote: ... and biotinylated detection antibody pairs from Invitrogen™ ...
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bioRxiv - Biophysics 2019Quote: ... 0.2 mg ml−1 biotinylated BSA (ThermoFisher) was added into the chamber and incubated for 3 min ...
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bioRxiv - Systems Biology 2019Quote: ... 4 µl Biotinylated Cytidine (Thermo Fisher, 1mM), 2 U RNase inhibitor ...
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bioRxiv - Molecular Biology 2023Quote: ... and biotinylated cytidine (bis)phosphate (Thermo scientific) using T4 RNA ligase enzyme from Thermo Fisher Scientific.
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bioRxiv - Biochemistry 2023Quote: ... followed by biotinylated BSA (Life Technologies Europe) diluted in BRB80 ...
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bioRxiv - Cell Biology 2020Quote: ... AHA-labeled proteins were detected with 1 μM alkyne-tagged Alexa Fluor-488 using Click-iT cell detection reagents (Thermo Fisher Scientific) for 45 min at room temperature as previously described (3).
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bioRxiv - Neuroscience 2021Quote: Bacterial expression constructs coding for His6/SUMO-tagged fusion proteins of rat Shank3 (residues 1-348, SPN-Ank) were generated in pET-SUMO (Thermo Fisher Scientific) as described before (17) ...
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bioRxiv - Molecular Biology 2021Quote: ... Co-purification of 6His-tagged PGC-1α protein domains was detected by western blot using anti-6His antibody (clone His.H8, ThermoFisher Scientific MA1-21315).
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bioRxiv - Cell Biology 2021Quote: ... and anti-TRIP4 antibodies were raised in rabbits against affinity purified N-terminally hexahistidine-tagged full-length proteins and purified with the respective antigens coupled to SulfoLink resin (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... SH-SY-5Y cells stably expressing GFP or a GFP-tagged construct of the protein of interest were cultured for at least six doublings in SILAC DMEM (89985; Thermo Fisher Scientific) supplemented with 10% dialyzed FBS (F0392 ...
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bioRxiv - Biophysics 2023Quote: RBL-2H3 cells stably expressing clathrin light chain A tagged with green fluorescent proteins were cultured in minimum essential medium (MEM) (Invitrogen 11095-09) with 20% fetal bovine serum (FBS ...
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bioRxiv - Systems Biology 2023Quote: ... ABE8e was expressed as an 8xHis tagged protein from a rhamnose-inducible promoter in BL21-Star DE3 cells with low RNase activity (Thermo Fisher Scientific). At an OD600 of ∼0.8 ...
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bioRxiv - Molecular Biology 2023Quote: ... GST pull-down of PARylated proteins using GST-tagged RNF146(100-182) or GST-tagged AF1521(K35E/Y145R) was done using the Pierce GST protein interaction pull-down kit (Thermo Fisher Scientific). Glutathione agarose resin (50μl ...