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Citations for Thermo Fisher :
1051 - 1100 of 10000+ citations for Recombinant Human BTLA Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: GST pull-downs were performed by incubating purified GST tagged proteins (5 µg) with 20 μl dry volume of glutathione agarose 4B beads (Thermo Scientific) for 1 h under rotation at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... or 90 pmol of SBP-tagged eIF4A1 protein was incubated with 30 μl of Dynabeads M-270 Streptavidin (Thermo Fisher Scientific), which had been preequilibrated with equilibration buffer (20 mM Tris-HCl pH 7.5 ...
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins (5 µg) along with GST alone were incubated with 10 µl of magnetic glutathione beads (Thermo Fisher Scientific) for one hour at RT ...
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bioRxiv - Microbiology 2024Quote: ... Western blotting and immunodetection of HA-tagged proteins with anti-HA and horse radish peroxidase-coupled secondary antibodies using an iBright™ FL1500 (ThermoFisher). The remaining 25 µl bead suspension was used as input for on-bead digestion (Klaus et al. ...
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bioRxiv - Immunology 2022Quote: ... Both recombinant LPS (100ng/ml) and recombinant IFNy (10 ng/ml, Gibco) were provided to cells for differentiation to classical macrophages ...
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bioRxiv - Molecular Biology 2020Quote: ... Immulon 2 HB plates were coated with 2μg/ml Pierce recombinant protein A/G (ThermoFisher catalog no: 77677) or purified SARS-CoV-2 receptor binding domain (provided by Florian Krammer ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was collected 6 days later and recombinant mAb was purified using protein A agarose (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: Recombinant STUB1 protein (aa25-aa153) was dialyzed overnight with Slide-A-Lyzer cassette (7K MWCO, Thermo Scientific, #66373) in 1 liter of dialysis buffer (PBS ...
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bioRxiv - Biophysics 2022Quote: ... The recombinant protein was expressed in the Pichia pastoris strain SMD1168H (Philosof et al., 2017) (Thermo Fisher Scientific). The cells were harvested 48–60 h after expression was induced in BMMY medium when 10 mM of all-trans-retinal (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant protein was captured on a Ni2+-NTA HisPur® affinity resin by gravity flow (Thermo Fisher Scientific). Unbound proteins were washed with binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... Expression and purification of recombinant proteins were performed according to manufacturer’s instructions (Invitrogen Corporation, Catalog no. K1710-01): YPD medium supplemented with 100 μg ml−1 zeocin was used for initial growth of P ...
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bioRxiv - Biochemistry 2020Quote: The soluble recombinant protein was captured on a Ni2+-NTA affinity resin by gravity flow (Thermo Fisher Scientific). Unbound proteins were washed with 25 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... Dinutuximab and dinutuximab LALA-PG were then purified using recombinant protein A-Sepharose 4B (ThermoFisher Scientific, Waltham, MA), buffer exchanged into antibody buffer (20mM L-Histidine ...
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bioRxiv - Microbiology 2022Quote: The recombinant proteins named rS1Beta was purified using a CaptureSelect™ C-tagXL Affinity Matrix prepacked column (ThermoFisher) and followed the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant Endocan and PDGF-BB were labeled with Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher Scientific) and Alexa Fluor™ 647 Microscale Protein Labeling Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: Recombinant soluble ICAM-1 protein was expressed in a Drosophila Melanogaster cells using pMT/V5-His vector with inducible promotor (Invitrogen), purified by sequential two-step affinity chromatography on anti-ICAM-1 antibody (clone YN1.1 ...
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bioRxiv - Immunology 2021Quote: ... The efficiency of the expression and purification of recombinant proteins were evaluated by 12% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:3,000) (Thermo Scientific) and HRP-conjugated goat anti-mouse IgG (1:10,000 ...
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bioRxiv - Microbiology 2019Quote: ... 1 nM of CAT protein (Chloramphenicol acetyltransferase, containing a 6×His tag at C-terminus) expressed in the same baculovirus-expression system (ThermoFisher) was used to coat plate wells ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The presence of AhR and ARNT proteins was verified by Western blot using anti-His-tag (mouse monoclonal, MA1-21315, dilution 1:1000, Invitrogen) anti-FLAG-tag (rabbit monoclonal ...
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bioRxiv - Genomics 2022Quote: ... The purified protein was analyzed by SDS Page electrophoresis and western blot analysis using anti-His antibodies as per manufacturer guidelines (ThermoFisher).
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bioRxiv - Plant Biology 2023Quote: ... His-tagged proteins were detected on film by chemiluminescence using a horseradish peroxidase-conjugated His antibody and SuperSignal West Femto substrate (ThermoFisher).
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bioRxiv - Bioengineering 2024Quote: ... Antibodies were purified by gravity column using Protein G Sepharose 4 Fast Flow Resin (Cytiva) and proteins containing His tags were purified on Ni-NTA Agarose (R90101 Invitrogen).
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bioRxiv - Neuroscience 2021Quote: ... Anthony White (QIMR Berghofer Medical Research Institute, Australia) were maintained on human recombinant vitronectin in StemFlex medium (Life Technologies, cat. # A3349401). Differentiation was performed as previously described [15 ...
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bioRxiv - Systems Biology 2020Quote: ... 72 h after seeding on 96-well microplates differentiated cells were incubated with recombinant human interferon gamma (IFN-γ, ThermoFisher, #PHC4031) at concentrations 0-10 ng/mL or recombinant human interleukin 10 (IL-10 ...
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bioRxiv - Cell Biology 2020Quote: ... coated overnight with 10 μg/ml of xeno-free human recombinant Laminin 521 (LN521, Biolamina) prepared in GMP DPBS+/+ (A1285801, Life Technologies) by reversing the strainer and washing the EBs into the plate with GMP N2 media (CTS™ DMEM-F12 ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated in RP-10 supplemented with 10 ng/ml recombinant human IL-2 and 2 μg/ml anti CD28 (clone 37.51, Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS)) and 30IU/mL recombinant human IL-2 (rhIL-2; Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cell Biology 2020Quote: ... HUVECs were treated with 30 ng/ml of VEGF (Recombinant Human Vascular Endothelial Cell Growth Factor, Fisher Scientific 10690920, Bordeaux, France) for 24 hours.
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bioRxiv - Immunology 2021Quote: Purified CD8+ cells were cultured in AIMV medium supplemented with 10% FBS and 100 U/ml recombinant human IL-2 (ThermoFisher Scientific) and used for sorting of CD8+ T cell subsets and for further experiments three days after isolation.
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bioRxiv - Cell Biology 2022Quote: ... 50 mM β- Mercaptoethanol and 1 ng/ml human recombinant leukemia inhibitory factor) for 24 h and then treated with 0.1 μg/ml Colcemid (Gibco, 15210-040) for 2.5 h ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ & mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ and mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
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bioRxiv - Cell Biology 2023Quote: Saturating amounts of recombinant human (rh) EGFR-Biotin and rhPD-L1-Biotin (ACRO Biosystems) were coated onto separate Streptavidin-coated DynaBeads (Thermo Fisher) using manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... were cultured in complete RPMI (10% FCS, 1% Penicillin/Streptavidin, 1% HEPES) supplemented with recombinant human M-CSF (10 ng/mL, Life Technologies) for 7-10 days to differentiate monocyte-derived macrophages (MDM) ...
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bioRxiv - Developmental Biology 2023Quote: Stem cells were grown on culture plates coated with 5micro μg ml-1 recombinant human Vitronectin (rhVTN-N, Life Technologies, #A14700). HESCs were grown in mTeSR1 (StemCell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... except for RWPE1 which were maintained in Keratinocyte Serum Free Medium supplemented with bovine pituitary extract and human recombinant epidermal growth factor (manufacturer supplied, Life Technologies). For spheroid cultures ...
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bioRxiv - Cancer Biology 2023Quote: H357 and H376 cells at ∼80 % confluence were treated either with distilled water (as control) or with 20 ng/ml of Human Recombinant FGF2 (PHG0026, ThermoFisher Scientific) for 48 h ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 20 U/mL recombinant human IL-2 at 37°C and 5% CO2 for 24 hours in RPMI 1640 (Gibco, Canada) containing 100 Units of penicillin and 0.1 mg/mL of Streptomycin (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... 6-well plates were coated with 20 µg/mL Human recombinant laminin-521 (Biolaminin; Biolamina; LN521-05) in 1x dPBS++ (Gibco; 14040117) and incubated overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 5 ng/ml human recombinant epidermal growth factor (EGF) and 50 µg/ml bovine pituitary extract (BPE)(all Thermo Fisher). Above cells were split every 4-5 days using 5-10-minute incubation with 0.05% trypsin-EDTA (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: Dynabeads His-Tag Isolation & Pulldown (Invitrogen) were washed in PBS-TH (PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-His (Thermo Fisher scientific, rd230540a), anti-EZH2 (Cell signaling #5246 ...
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Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cellsbioRxiv - Cell Biology 2022Quote: ... pcDNA3.1/V5-His (Thermo Fisher Scientific), pCLXSN-GFP (Reiley et al 2005) ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% HI-FBS (Gibco) at 37°C in the presence of 5% CO2 ...
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bioRxiv - Genetics 2019Quote: ... 2% HI horse serum (Thermo Fisher), and 1% P/S ...
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bioRxiv - Immunology 2021Quote: ... magnetic beads (Dynabeads His-Tag, Invitrogen) were coated with histidine tagged S protein according to the manufacturers’ instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Hi-Di Formamide (Applied Biosystems, California) and 500 liz size standard (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... with 10% HI-FBS (Gibco, #10082147) and 1% Penicillin-Streptomycin (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... Stock anti-His antibody (Thermo Fisher) was diluted to 70 μg/mL in BRB80 buffer ...
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bioRxiv - Microbiology 2022Quote: ... Mouse-anti-His (HIS.H8) antibody (Invitrogen) (for eGFP/His-tagged NP protein co-immunoprecipitation ...