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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for Puumala Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% foetal calf serum (FCS) (Gibco) and 1% GlutaMAX (200mM ...
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bioRxiv - Developmental Biology 2023Quote: ... FLs were recovered in HBSS+1% FCS (Gibco) and passed through a 26-gauge needle of a 1-ml syringe to obtain single-cell suspensions ...
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bioRxiv - Immunology 2023Quote: ... and dialyzed FCS were bought from Thermo Fisher. Interferon γ (IFNγ ...
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bioRxiv - Microbiology 2023Quote: ... with 10% foetal calf serum (FCS) (Thermo Fisher) and 1% penicillin/ streptomycin (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... and 5% FCS (Thermo Fisher Scientific, Waltham, USA)] to quench the trypsin and dissociated into a single-cell suspension by gentle repeated passages through a sterile glass pipette.
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bioRxiv - Bioengineering 2022Quote: ... 100 mg/ml foetal calf serum (FCS, Invitrogen) and an antibiotic-antimycotic agent (15240-062 ...
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bioRxiv - Cell Biology 2022Quote: ... containing 10% fetal calf serum (FCS; Life technologies), 1% penicillin-streptomycin (10,000 U/ml penicillin ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% (v/v) HI-FCS (Gibco), 50 μM mercaptoethanol (Sigma-Alrich) ...
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bioRxiv - Cell Biology 2023Quote: ... containing 5 % fetal calf serum (FCS, Life Technologies) and 2 mM L-glutamine ...
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bioRxiv - Immunology 2023Quote: ... and 10 % FCS (Thermo Fisher Scientific, Waltham, USA) at 37°C and 5 % CO2.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... a multiskan FC spectrophotometer (Thermo Scientific, Illkirch, France) was used to measure the optical density at 620 nm.
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FCS (Thermo Scientific, SH30070.03 Hyclone), 55 μM 2-ME (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10 % fetal calf serum (FCS, Gibco, ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Multiplexed samples were incubated in Fc block (Invitrogen) for 10 min at RT ...
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bioRxiv - Cell Biology 2024Quote: ... and 10% FCS (all from Thermo Fisher Scientific). Stable HeLa cell lines were generated using the T-Rex doxycycline inducible Flp-In system (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... and lanes reacted with S-tag antibody were incubated in rabbit anti-mouse S-tag antibody (Invitrogen PIPA 581631, 1:500 in TBST). Blots were washed 8 × 5 minutes in TBST and then ladder lanes were reacted with Precision Plus Strep Tactin HRP Conjugate (BioRad 1610380 ...
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bioRxiv - Biochemistry 2020Quote: GC-1spg cells with inducible DAZL expression were maintained in DMEM high glucose medium (ThermoFisher) supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2020Quote: ... GCs were collected by filtration of the remaining cells through 40μM cell strainers (Fisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... Genotype information of Singapore GC patients was extracted from the .CEL files produced from Affymetrix Genome-Wide Human SNP 6.0 Arrays (GSE31168 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the samples were analyzed on TRACE 1300 GC-MS system (Thermo Fisher Scientific, MA, USA) equipped with AI1310 autosampler and Thermo TG-5MS column (length ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Each C-terminally HA tagged GC-C variant was synthesized via gene synthesis (Life Technologies). GC-C was then cloned into the lentiviral transfer vector pUltra (Addgene #24129 ...
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bioRxiv - Microbiology 2021Quote: The samples above were detected using DSQ II single quadrupole GC-MS (Thermo Fisher Company). The injection port temperature of the instrument was maintained at 250 °C ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected either with a control siRNA (Stealth Negative Control Medium GC Duplex, Invitrogen) or with siPFKFB4 (Dharmacon Smartpool siGenome D-006764-01/02/04/17 ...
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bioRxiv - Microbiology 2023Quote: ... Concentrations of the gaseous compounds were monitored automatically with a TRACE 1310 GC (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Stealth RNAi Negative Control Medium GC (cat. no. 12935-300; Invitrogen; Thermo Fisher Scientific, Inc.) was used as the negative control ...
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bioRxiv - Cancer Biology 2023Quote: ... Stealth RNAi Negative Control Medium GC (cat. no. 12935-300; Invitrogen; Thermo Fisher Scientific, Inc.) was used as the negative control ...
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bioRxiv - Pathology 2024Quote: ... Transfection of GC cell lines was conducted using Lipofectamine RNAiMAX (#13778075, Invitrogen, Waltham, Massachusetts, USA) following previously described methods (25) ...
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bioRxiv - Developmental Biology 2024Quote: ... a stealth RNAi negative control medium GC duplex was purchased from Invitrogen (Carlsbad, CA, USA). Predesigned siRNAs targeting human FLNA (#2316-1 ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
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bioRxiv - Genomics 2020Quote: ... cells were washed twice in FC buffer and anti-mouse Fc Alexa Fluor 647-conjugated secondary antibody (Thermo Scientific, A-21236) was added at 1:500 dilution ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Random primer (fc 50 ng/µl) and RNase Inhibitor (fc 1 unit/µl) in 1X First Strand synthesis buffer (Invitrogen/Life Technologies). Gene expression was performed on a BioRad CFX96 real time PCR machine using gene-specific FAM-labelled TaqMan probe assays ...
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bioRxiv - Neuroscience 2022Quote: Recombinant NRX1β-Fc and NRX1βΔHRD-Fc proteins were pre-immobilized with Protein G Magnetic Beads (Dynabeads Protein G, Cat# 10004D, Thermo Fisher Scientific) in PBS overnight at 4 °C with constant agitation ...
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bioRxiv - Cell Biology 2023Quote: Cells in the exo-erythrocytic development assays were subsequently stained with a primary antibody against Plasmodium berghei HSP70 (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and an anti-mouse Alexa Fluor 488 (1:300 diluted in 10% FCS/ PBS; Life technologies). Hoechst 33343 was added after for 5 min at RT and the cells washed twice in 1% FCS/ PBS the assays before being mounted ...
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bioRxiv - Cell Biology 2023Quote: Cells in the invasion assay were stained subsequently for 1 h at 37°C with primary mouse αCSP antibodies (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and anti-mouse Alexa Fluor 488 antibodies (1:300 diluted in 10% FCS/ PBS; Life technologies). Following two washing steps with 1% FCS/PBS the cells were permeabilized by addition of ice-cold methanol for 10 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Subsequently another round of blocking with 10% FBS/ PBS at 37°C or at 4°C overnight was followed by a second round of subsequent incubation with αCSP (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and secondary anti-mouse Alexa Fluor 546 antibodies (1:300 diluted in 10% FCS/ PBS; Life technologies) for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Glycosylation was detected by in-gel periodic acid-Schiff staining using the Pro-Q Emerald 488 glycoprotein staining kit (Invitrogen, Carlsbad, CA, USA) and imaged using a Typhoon FLA 9000 laser bed scanner (GE Healthcare Bio-Sciences ...
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bioRxiv - Biochemistry 2022Quote: ... Prostate cancer cell lines were cultured in RPMI medium supplemented with 2 mM L-glutamine and 10% heat-inactivated FCS (ThermoFisher Scientific, Melbourne, VIC, Australia). Cell media was changed every three days and cells were passaged regularly at ∼80-90% confluency by trypsinization ...
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bioRxiv - Microbiology 2024Quote: ... and human (goat anti-human IgG, ThermoFisher) were also used ...
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bioRxiv - Immunology 2021Quote: ... T-cells were stimulated with Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific) in the presence of PBMC-derived CECs (ratio 1:2 ...
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bioRxiv - Systems Biology 2020Quote: ... Caco-2 human colorectal adenocarcinoma (ATCC HTB-37) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (GibCo) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Bioengineering 2020Quote: Human colon adenocarcinoma cells (Caco-2) were cultured in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... and Rabbit anti-Human IgG F(ab’)2 HRP Secondary Antibody (ThermoFisher Scientific Catalog #31482, 1:10,000).
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bioRxiv - Cancer Biology 2021Quote: ... containing 2 datasets in which 163 samples were analyzed using the GPL96 (Affymetrix Human Genome U133A Array) platform and 80 samples were analyzed using GPL570 (Affymetrix Human Genome U133 Plus 2.0 Array ...
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bioRxiv - Microbiology 2022Quote: ... The remainder was stained using FITC-conjugated F(ab’)2-Goat anti-human IgA (Invitrogen; 1/1000) in PBS + 0.1% BSA (Fraction V ...
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bioRxiv - Microbiology 2022Quote: ... mNAb binding was detected using horseradish peroxidase–labelled goat anti-human IgG F(ab’)2 (Thermofisher-Scientific), or horseradish peroxidase–labelled anti-human IgA ...
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bioRxiv - Microbiology 2023Quote: HeLa cells (Human epithelial cells, ATCC CCL-2) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco), supplemented with heat-inactivated fetal calf serum (10%) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were activated with 30 U/mL rIL-2 and Dynabeads Human T-activator CD3/CD28 (ThermoFisher) and cultured with the CM of CA-MSCs in the presence or not of CXCR4 Ab (R&D Systems) ...
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bioRxiv - Biophysics 2023Quote: ... human IL-2 (Cat# PHC0026) and CellTrace™ CFSE (Cat# C34570) were purchased from ThermoFisher (Waltham, MA). Bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... Human HK-2 cells were cultured in DMEM and Keratinocyte-SFM (1X) (Life Technologies Green Island NY) media ...
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bioRxiv - Systems Biology 2024Quote: Human osteosarcoma epithelial cells (U-2 OS cell line) were cultivated in DMEM high glucose medium (Gibco), supplemented with 2 mM L-glutamine ...