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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for Puumala Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... with 10% fetal calf serum (FCS, Gibco) and penicillin-Streptomycin (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with decomplemented FCS 10% (ThermoFisher Scientific) and 1% penicillin/streptomycin (Merck) ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with decomplemented FCS 10% (ThermoFisher Scientific) and 1% penicillin/streptomycin (Merck ...
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bioRxiv - Cancer Biology 2024Quote: ... containing 5% dextran-charcoal treated FCS (Invitrogen), MCF7 (RRID:CVCL_0031 ...
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bioRxiv - Cell Biology 2023Quote: ... 10% fetal calf serum (FCS, Gibco 10270), and with both penicillin (100μg/mL ...
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bioRxiv - Immunology 2023Quote: ... + 10% FCS (Benchmark) + Penicillin/Streptomycin + Glutamine (Gibco). TCR stimulation was induced by adding anti-CD3/CD28 Dynabeads (Applied Biosystems ...
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bioRxiv - Systems Biology 2024Quote: ... supplemented with 10% (v/v) FCS (Gibco),0.1μM dexamethasone ...
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bioRxiv - Cancer Biology 2024Quote: ... with 10 % fetal calf serum (FCS, Gibco) and Antibiotic-Antimycotic® (Gibco)) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% inactivated FCS (Gibco/Thermo), 1% penicillin/streptomycin (Gibco/Thermo ...
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bioRxiv - Genomics 2023Quote: ... with 10% foetal calf serum (FCS; ThermoFisher), 2 mM L-glutamine ...
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bioRxiv - Microbiology 2024Quote: ... and 10% fetal calf serum (FCS; Gibco). During infection experiments ...
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bioRxiv - Microbiology 2024Quote: ... with 10% foetal calf serum (FCS; ThermoFisher). Supernatants were harvested 5 days post infection (dpi ...
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bioRxiv - Physiology 2024Quote: ... containing 5 % fetal calf serum (FCS; Gibco) and 1% streptomycin and penicillin in gelatin (1% ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1 ml of 10% FCS (Gibco) in 99 ml of DMEM (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% vol/vol FCS (GIBCO). Spleens were mashed through a 100 μm cell strainer (Falcon) ...
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bioRxiv - Molecular Biology 2020Quote: ... T cells were stimulated for 2 days with anti-human CD3/CD28 magnetic Dynabeads (ThermoFisher) at a bead to cell concentration of 1:1 ...
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bioRxiv - Microbiology 2022Quote: ... human Anti-SARS COV-2 Nucleocapsid monoclonal (E16C) (Thermo Fisher, MA1-7403, 0.1 mg/mL), was incubated as a 1:100 dilution ...
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bioRxiv - Microbiology 2020Quote: ... codon-optimized (for human cells) SARS-CoV-2 DNA (GeneArt Gene Synthesis, Thermo Fisher Scientific) based on the publicly available protein sequence in the National Center for Biotechnology Information database (NCBI Reference Sequence ...
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bioRxiv - Neuroscience 2021Quote: The human iPSC lines iPSC-1 XMO01 (67) and iPSC-2 (A18945, Thermo Fisher Scientific) were cultured in standard conditions (37°C ...
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bioRxiv - Microbiology 2021Quote: ... 90% N2 in a 2% suspension of human red blood cells in RPMI medium (Gibco) modified with the addition of 30 mM NaHCO3 ...
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bioRxiv - Cell Biology 2023Quote: Reconstitute Human FGF-basic (FGF-2/bFGF) Recombinant Protein (here we use ThermoFisher 13256-029). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were pretreated for 1 h with 10 ng/mL human recombinant FGF-2 (Gibco) or vehicle (sterile water) ...
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bioRxiv - Immunology 2023Quote: ... in presence of Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, from Thermofisher, USA) and IL-2 (50 U/mL ...
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bioRxiv - Bioengineering 2024Quote: ... Human umbilical vein endothelial cells (HUVECs) were expanded in Endothelial Growth Medium 2 (Fisher Scientific) until confluent ...
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bioRxiv - Immunology 2022Quote: ... samples were resuspended in binding buffer containing a 1:100 dilution of mouse anti-human-IgG1-Fc conjugated to Alexa Fluor 488 (Thermo Fisher Scientific, Rockford, IL; A-10631) and incubated for 30 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2022Quote: ... 1% PenStrep seeded in 10-cm dishes were transfected with the plasmid encoding for the corresponding S glycoprotein using lipofectamine 2000 (Life Technologies) following manufacturer’s indications ...
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bioRxiv - Immunology 2021Quote: ... 1% PenStrep seeded in 10-cm dishes were transfected with the plasmid encoding for the corresponding S glycoprotein using lipofectamine 2000 (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The presence of glycoproteins was examined by staining the gels with SYPRO Ruby protein gel stain according to the manufacturer’s instructions (Thermo Fisher Scientific). Stained LPS and protein bands were visualized under UV light using an Azure c300 gel imaging system (Azure Biosystems).
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bioRxiv - Microbiology 2020Quote: ... New York University) and a pcDNA3.1 plasmid expressing the SARS-CoV2 glycoprotein into HEK 293T cells using Lipofectamine 3000 (Thermo Fisher Scientific). The supernatant was collected 48 h after transfection ...
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bioRxiv - Microbiology 2021Quote: ... ss-HA-Spike plasmids (HA tag at the N terminus with a Serine-Glycine linker between residues S13 and Q14 of S glycoprotein (Stewart et al., 2021)) were transfected using Lipofectamine 3000 (ThermoFisher Scientific), following the manufacturers guidelines ...
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bioRxiv - Immunology 2021Quote: ... 1% PenStrep seeded in 10-cm dishes were transfected with the plasmid encoding for the corresponding S glycoprotein using lipofectamine 2000 (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... HEK-293T cells seeded in poly-D-lysine coated 100 mm dishes at ∼75 % confluency were washed five times with Opti-MEM and transfected using 24 µg of the S glycoprotein plasmid with Lipofectamine 2000 (Life Technologies). After 5 h at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% PenStrep seeded in 10-cm dishes were transfected with the plasmid encoding for the corresponding S glycoprotein using lipofectamine 2000 (Life Technologies) following manufacturer’s indications ...
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bioRxiv - Immunology 2022Quote: ... HEK-293T cells seeded in poly-D-lysine coated 100 mm dishes at ∼75 % confluency were washed five times with Opti-MEM and transfected using 24 μg of the S glycoprotein plasmid with Lipofectamine 2000 (Life Technologies). After 5 h at 37°C ...
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bioRxiv - Immunology 2022Quote: EAE was induced in 8-12 week old mice by subcutaneous injection with 100 μL of inoculant containing 100μg myelin oligodendrocyte glycoprotein peptide fragment 35-55 (MOG35-55, Thermo Scientific J66557MCR) in PBS with complete Freund’s adjuvant (CFA ...
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bioRxiv - Microbiology 2023Quote: ... Gels have been stained with silver to observe the total protein profile or with Pierce™ Glycoprotein Staining Kit (Thermo Fisher) to detect glycoprotein sugar moieties according to the manufacturer’s instruction.
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bioRxiv - Immunology 2023Quote: ... 2·105 Tag-it Violet-labelled splenocytes were cultured in 96-well U-bottom plates (Nunclon Delta Surface; Thermo Scientific, Waltham, MA, USA) at a final volume of 200 µL of supplemented IMDM per well ...
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bioRxiv - Immunology 2021Quote: ... 1:100 human IgG (1 mg/ml) as FcR block and 2 % FCS using the following staining reagents: 7-AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
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bioRxiv - Cell Biology 2021Quote: ... Akata cells were grown at 37°C under 5% CO2 in RPMI-1640 supplemented with 10% FCS and 2 mM of L-Glutamine (Gibco, 25030-081). Cells KD for BHRF1 were generated from Akata cells (see below ...
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bioRxiv - Immunology 2022Quote: ... were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies, 25030-024], 1 mM sodium pyruvate [Gibco Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... spun at 200 × g for 10 min and resuspended in macrophage medium (DMEM [Invitrogen] plus 10% FCS and 2 mM L-glutamine) in order to have 2.5 × 106 cells per well ...
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bioRxiv - Immunology 2020Quote: ... Staining was performed on ice for 25 minutes in PBS with 2 % FCS using the following antibodies: 7- AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% FCS (BioConcept, Cat# 2-01F16-I) and 100 units/ml of penicillin and 100 µg/ml of streptomycin (Invitrogen, Cat# 15140122). Rat primary fibroblasts (REFs ...
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bioRxiv - Immunology 2023Quote: ... Isolated T-cells were resuspended in complete RPMI (RPMI 1640 [Gibco, #21870-076] supplemented with 2% FCS and 100x Penicillin-Streptomycin [Gibco, #10378-016]).
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bioRxiv - Developmental Biology 2023Quote: ... with 10% FCS (Biosera, #FB-1280/500) and 2% glutamate/pyruvate (100mM sodium pyruvate, Invitrogen #11360-039; 200mM L-glutamine, Invitrogen, #25030-024). Slides were imaged for brightfield ...
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bioRxiv - Molecular Biology 2021Quote: ... we cloned an EcoCas3 cDNA with a octa-histidine tag and a six asparagine-histidine repeat tag into a pFastbac-1 plasmid (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... ACE2-S-tag expression was detected by using a mouse anti-S-tag monoclonal antibody 6.2 (Invitrogen, Cat. No. MA1-981), and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... cDNA encoding mouse TCR 2B4 α (with c-Myc-tag) and β (with V5-tag) sequences with 2A sequence linking each other were cloned into pCDNA3.1/Zeo(+) vector (Life Technologies) using Not1 and Xho1 restriction enzymes ...
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bioRxiv - Biochemistry 2021Quote: ... Coverslips were washed three time with PBS before the incubation with Alexa Fluor 555 (for Myc tag) and/or Alexa Fluor 647 (for HA tag) conjugated secondary antibodies (Invitrogen/Thermo Fisher) diluted in PBSAT for 30 min at RT ...
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bioRxiv - Immunology 2020Quote: ... PR8-HA and SARS-CoV-2 S proteins with C-terminal Avi-tags and His-tags were expressed via transient transfection of Expi293 suspension cultures (Thermo Fisher) and purified by polyhistidine-tag affinity and size exclusion chromatography ...