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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for 5R 3 4 5 6 Tetrahydro 5 phenyl N benzyloxycarbonyl 4 H 1 4 oxazin 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... The iPSCs were dissociated (DD0) after 4-5 days of maintenance using 0.25% trypsin / 1 mM EDTA (Thermo Fisher Scientific) at 37°C for 2 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and 8 mM MgSO4 pH 7.0 (2x PHEM buffer) containing 1% Triton X100 for 5 min and then fixed in 4% (v/v) paraformaldehyde (ThermoFisher) in 2x PHEM buffer at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... was reconstituted by submerging the vial at 4°C over night and was diluted 1:5 in ice-cold knock-out DMEM (Cat.-No. 10829, Gibco) until material was evenly dispersed ...
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bioRxiv - Developmental Biology 2023Quote: ... Brains were then washed using 0.5% PBT (4×15 minutes) and incubated 1:500 with secondary antibody (anti-mouse/rabbit Alexa-488/647, Invitrogen/ThermoFisher) for 4 hours at RT before transferring to 4°C for three overnights ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was then incubated in primary antibody in 5% milk and TBS-T overnight at 4°C at the following dilutions: 1:1000 rabbit αFLAG polyclonal (Invitrogen) and 1:1000 rabbit αHsp70 polyclonal (Abcam) ...
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bioRxiv - Neuroscience 2022Quote: ... Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568, 1:200, Invitrogen, A11041 ...
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bioRxiv - Neuroscience 2022Quote: ... Brain sections were incubated in blocking solution (0.2% Triton X-100 in PBS with 5% normal goat serum) for 1 hr at 4°C and then with a primary antibody anti-GFP (Invitrogen, catalog # G10362 ...
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bioRxiv - Microbiology 2023Quote: Cells were resuspended in PBS and incubated at 4 °C for 5 mins with 8 µg ml−1 Alexa Fluor 555 NHS ester (Invitrogen). Cells were then washed in PBS.
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bioRxiv - Neuroscience 2024Quote: ... pH = 7.6) followed by an overnight incubation at 4°C with 1:1500 anti-mouse Tau-5 antibody (Invitrogen #AHB0042) in superblock blocking buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 16 mL of HMA-10 buffer was added and the preparation was put on a shaking rocker for one hour at 4 °C with 3 units/mL Rnase-free Dnase (Ambion) added ...
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bioRxiv - Neuroscience 2024Quote: ... 3% dilution, 4 µl,), Fluoro-ruby (FR, case BC, (10% solution, mixture of 10 kDA and 3 kDA, 4 µl; Invitrogen), and Biotinylated Dextran Amine (BDA ...
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bioRxiv - Immunology 2023Quote: Following euthanasia, adult zebrafish heads (15 wpf, N=3) were fixed in a solution of 4% methanol-free formaldehyde (Thermofisher) in 60 mM HEPES buffer (pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved on precast NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). Samples were transferred to 0.45 µm nitrocellulose membrane (0.9 A ...
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bioRxiv - Cell Biology 2022Quote: ... at 180V on precast either NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen), or NuPAGE™ 3-8% Tris-Acetate gels in NuPAGE™ Tris-Acetate SDS Running Buffer (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were treated with either DMSO or 5 μM GolgiTracker ((BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine)) (Invitrogen. Cat# D3521) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... 2 M thiourea and 4% CHAPS (Thermo Fisher Scientific). Each combined replicate of samples was loaded onto a QIAshredder spin column (QIAGEN ...
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bioRxiv - Neuroscience 2021Quote: ... flash frozen in 2-methylbutane (Fisher Scientific, 03551-4), and kept at −80°C until sliced on a cryostat (Leica ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 mL of cold 4% PFA (Thermo Scientific, 28908) in RNAse-free PBS (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... and resuspended in 0.1 ml of PBS supplemented with the lipophilic styryl membrane dye N-(3-triethylammoniumprpyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64, Molecular Probes, Invitrogen; 10 μg.ml-1) (Pogliano et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... final concentration: 5 µM) and 4 µL of 1 mg/mL Hoechst 33342 dye (1:500 dilution) (Life technologies, Eugene, OR, USA) and resuspended thoroughly using a low binding pipette tip ...
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bioRxiv - Developmental Biology 2022Quote: ... The membrane was washed 5 times 5 min in TBS-T prior to overnight incubation at 4°C with 1° anti-Flag antibody (1:500, ThermoFisher, PA1-984B). The next morning ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysis buffer (20 mM Tris pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1% Triton X-100, 1 mM DTT, 25 U/ml DNase TURBO, Invitrogen cat#AM2238) had been added to the frozen monolayer ...
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bioRxiv - Immunology 2020Quote: ... Serial frozen sections (6 μm in thickness) were cut on a cryostat and counterstained with DAPI (4′,6-Diamidine-2′-phenylindole; Thermo Fisher Scientific). The number of beads in the SED from 3-4 sections of two Peyer’s Patches per mouse (n=3–4 mice/group ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 mmol/l 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Gibco, Germany #15630056), 2.5 ng/ml human fibroblast growth factor-basic (hFGF2 ...
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bioRxiv - Biochemistry 2021Quote: ... and 1:4 pD614G-SARS-CoV-2-spike:pPyEGFP using Lipofectamine 2000 (Life Technologies), and media was replaced on day 1 ...
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bioRxiv - Cell Biology 2022Quote: ... and 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (Invitrogen, Life Technologies, Grand Island, NY) supplemented with 5% human serum (Valley Biomedical) ...
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bioRxiv - Cell Biology 2022Quote: ... and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (Invitrogen, Life Technologies, Grand Island, NY) supplemented with 10% human serum (Valley Biomedical ...
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bioRxiv - Cell Biology 2022Quote: ... and 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (Invitrogen, Life Technologies, Grand Island, NY) supplemented with 5% human serum (Valley Biomedical) ...
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bioRxiv - Cell Biology 2022Quote: ... and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (Invitrogen, Life Technologies, Grand Island, NY) supplemented with 10% human serum (Valley Biomedical ...
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bioRxiv - Neuroscience 2024Quote: ... containing 1mM Probenecid and 20mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Gibco). Cells were imaged at Ex/Em = 490/525nm for Cal-520 AM fluorescence intensity for a total of 120 seconds ...
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bioRxiv - Biophysics 2024Quote: ... and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 6 - diamidino-2-phenylindole (DAPI, 5 µg/ml, Life Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... and one wash with 50 μl of 5× Maxima H RT buffer (Thermo Fisher). Finally ...
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bioRxiv - Immunology 2022Quote: ... for 4 h and the FOXP3 / Transcription Factor staining buffer set (Invitrogen) was used according to manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... for 12-16 h at 4°C followed by 0.05% trypsin (Gibco) for 10-20 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... After 4 h incubation with Protein AAgarose (20333, Thermo Scientific, Shanghai, China) at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... at 4°C for 24 h in 1.5 mL DMEM (Gibco #11995065) with dispase II (2.1 U/mL ...
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bioRxiv - Microbiology 2020Quote: ... The sporozoite-infected culture was maintained for 3 or 5 or 7 days after which the cells were fixed with 4% paraformaldehyde (ThermoFisher Scientific: catalogue number 28906) for 10 minutes ...
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In vivo imaging of the kinetics of microglial self-renewal and maturation in the adult visual cortexbioRxiv - Neuroscience 2020Quote: ... Wako 019-19741) followed by secondary antibody solution (4 h, RT, AlexaFluor 488, 1:500, Invitrogen), mounted and coverslipped ...
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bioRxiv - Neuroscience 2023Quote: ... for 72 h at 4°C and secondary antibody anti-mouse Alexa-488 (1:300, Invitrogen).
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bioRxiv - Synthetic Biology 2024Quote: ... The supernatant was incubated at 4 °C for 1 h with HisPur Ni-NTA resin (ThermoFisher), after which the samples were applied twice to a gravity flow column at room temperature ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and then 1 h at 4°C at 7-10 rpm with dynabeads protein A (Invitrogen), equilibrated with the EB ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were mounted in antifade mounting medium with 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) and visualized with IX81 confocal microscope (Olympus ...
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bioRxiv - Molecular Biology 2021Quote: ... and mounted on slides with Prolong Diamond Antifade Mountant with 4′,6-diamidino-2-phenylindole (DAPI) (Molecular Probes). Imaging was performed using a Nikon A1 Confocal microscope.
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bioRxiv - Neuroscience 2020Quote: ... the tissues were counterstained with DAPI (4’,6-diamidino-2-phenylindole) and mounted with Prolong Gold (Life Technologies). Fluorescence was detected using an epifluorescence microscope (Nikon Eclipse NiE).
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bioRxiv - Bioengineering 2020Quote: ... Cells migrating through the porous membrane are identified by staining with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen), counting five representative fields per insert ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were washed in TNT and stained with 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen, catalog # D3571), washed again ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were mounted on slide using Antifade mounting media with DAPI (4-6-diamidino-2-phenylindole; ThermoFisher). After sealing the coverslips with clear nail polish ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) in ProLong Diamond Antifade Mountant (Thermo Fisher # P36971). Images were captured using a Nikon Eclipse Ti2 microscope ...
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bioRxiv - Microbiology 2021Quote: ... Cover slips were mounted using Prolong Gold Antifade reagent containing 4’,6-diamidino-2-phenylindole (DAPI; Life Technologies) and incubated overnight at 37°C ...