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Citations for Thermo Fisher :
1051 - 1100 of 1123 citations for Canine Parainfluenza Virus Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Culture medium was replaced with lentivirus-containing medium at the ratio of 100 μL virus-containing medium per 25,000 cells seeded in the presence of 6 μg/mL polybrene (Thermo Fisher Scientific #TR1003G), followed by centrifugation at 2,000 rpm in room temperature for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... These plasmids were co-transfected with MLV (murine leukemia virus)-CMV (cytomegalovirus) luciferase and MLV Gag/Pol plasmids into HEK-293T cells (ATCC CRL-3216) using Lipofectamine 2000 (Thermo Fisher 11668019) transfection reagent following the manufacturer’s recommended protocol ...
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bioRxiv - Immunology 2022Quote: ... and subjected to the one-step quantitative real-time reverse transcription-PCR assay (qRT-PCR) using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, Cat# 4444432) as described previously58 ...
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bioRxiv - Microbiology 2023Quote: ... virus was serially diluted in Vero maintenance media (MEM supplemented with 2% FBS and 1% each of GlutaMax (Gibco, Grand Island, NY), PenStrep ...
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bioRxiv - Microbiology 2023Quote: ... First-strand cDNA synthesis was performed on 1 µg of total RNA with the RevertAid H Minus Moloney murine leukemia virus (M-MuLV) reverse transcriptase (Thermo Fisher Scientific) using random primers p(dN)6 (Roche) ...
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bioRxiv - Immunology 2023Quote: ... The next day cells were infected with VSV-GFP virus at multiplicity of infection (MOI) of 1 in serum-free DMEM (DMEM Thermo Fisher, 11965092). After 1hr of incubation with media containing virus ...
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bioRxiv - Cell Biology 2023Quote: ... The virus supernatant was collected after 24 hrs and 48 hrs and filtered with a 0.45-μm syringe filter (Thermo Fisher, CA, USA); polyethylene glycol (PEG ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We added 50 larvae that have orally ingested half their body lengths of virus solution to 2 25-cell compartmentalized square petri dishes (ThermoFisher Scientific, U.S.A.) with ‘standard’ food and incubated them for 20 days [38] ...
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bioRxiv - Plant Biology 2024Quote: ... 1.0 μg DNase-treated RNA was reverse transcribed in a reaction volume of 20 μl containing oligo-(dT)19 primer and Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific). RT-qPCR analysis was then performed with 1.0 μl of 5-fold diluted cDNA using a SYBR premixed Ex Taq kit (Takara) ...
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bioRxiv - Microbiology 2024Quote: ... and after one hour virus was removed and cells were washed in PBS once before replacing culture media (DMEM F-12 (Thermo Fisher Scientific), penicillin (100LIU/ml)/streptomycin (100Lμg/ml ...
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bioRxiv - Microbiology 2024Quote: ... levels quantitative real-time PCR (RT-qPCR) was performed as previously described22 using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, #4444436) and an OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Molecular Biology 2020Quote: ... a female human embryonic kidney cell line transformed with the SV40 large T antigen was obtained from ATCC (#CRL-1573) and cultured in complete media consisting of DMEM (#11965092, GIBCO, Life Technologies; Carlsbad, CA) supplemented with 10% FBS (Seradigm Premium Grade HI FBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... rehydrated and underwent antigen unmasking by heat-induced epitope retrieval in 1× citrate buffer (pH 6.0; Thermo Scientific, Waltham, MA, USA #AP-9003-500). The sections were rinsed with PBS and blocked for 2 hours at room temperature using 10% normal donkey serum (NDS ...
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bioRxiv - Bioengineering 2020Quote: ... The specificity of the signals of antibodies against specific antigens was determined by performing a control experiment using compensation beads (Invitrogen, Cat# 01-1111-42).
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bioRxiv - Physiology 2020Quote: ... Antibodies to the antigen peptide were purified from the final bleed antiserum by affinity-purification using the AminoLink Plus Immobilization Kit (ThermoFisher Scientific, Waltham, MA, USA), with bound antibodies eluted using glycine elution buffer [6.3 ml of 100 mM glycine (VWR Chemicals ...
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bioRxiv - Immunology 2022Quote: ... Fluorescent antigen probes were used to detect VRC01gHL B cells with eOD-GT8 60-mer conjugated to Alexa Fluor 647 (Thermo Fisher Scientific, Catalog # A20173). Intracellular staining was performed with a fixation kit (BD Bioscience ...
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bioRxiv - Cell Biology 2023Quote: ... The solution was adjusted to 5 µg ml-1 in a total of 100 µl per well diluted antigen and were coated into Pierce™ Nickel Coated Plates (Thermo Fisher Scientific, #15142) at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... from singly sorted antigen-specific memory B cells were RT-PCR amplified using SuperScript III and AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Waltham, MA) under conditions previously described46.
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bioRxiv - Molecular Biology 2021Quote: ... A similar protocol was followed for small scale virus production when establishing individual stable cell lines with transfection being performed on 6-well plates using Lipofectamine 2000 (Thermo Fisher Scientific, 11668019) reagent.
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bioRxiv - Microbiology 2021Quote: ... Each 10-μl RT-qPCR mixture consisted of 2.5 µL 4X TaqMan Fast Virus 1-Step Master Mix (Life Technologies, Carlsbad, CA, USA), 400 nM each primer ...
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bioRxiv - Cell Biology 2021Quote: ... the extracellular RSV virus were collected by PEG6000 precipitation method and intracellular RSV virus were collected by lysing the macrophage cells with the lysis buffer from mirVanaTM miRNA Isolation Kit (Ambion, Life Technologies, AM1560). Total RNA from RSV infected macrophage cells as well as stock virus was extracted using mirVanaTM miRNA Isolation Kit according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: The full-length cDNA for HA or a mutated gene and neuraminidase genes of influenza A/Switzerland/9715293/2013 (H3N2) virus were amplified by using SuperScript One-Step RT-PCR (Invitrogen, Grand Island, NY), and the 6:2 recombinant viruses with six internal genes of influenza A/PR/8/1934(H1N1 ...
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bioRxiv - Microbiology 2020Quote: The TaqMan real-time RT-PCR assays were performed by using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, MA, USA). Each 20 μl reaction mix contained 5 μl of 4×Fast Virus 1-Step Master Mix ...
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bioRxiv - Immunology 2020Quote: ... and cDNA was reverse transcribed using the Moloney murine leukemia virus reverse transcriptase and random hexamer oligonucleotides for priming (Life Technologies, CA, USA). The expression of the genes of hexokinase 2 (HK2 ...
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bioRxiv - Immunology 2020Quote: 0.5 - 1 μg of total RNA was prepared by column purification (MachereyNagel) and transcribed into cDNA using Moloney murine leukemia virus reverse transcriptase (RevertAid Reverse Transcriptase, Thermo Fisher Scientific, #EP0441) in a total volume of 10 or 20 μl ...
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bioRxiv - Physiology 2020Quote: ... One microgram of RNA was reverse transcribed into cDNA using oligo-dT and MMLV-RT (Moloney Murine Leukemia Virus Reverse Transcriptase, Invitrogen, Thermo Fisher Scientific). Before cDNA synthesis ...
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bioRxiv - Microbiology 2020Quote: ... diluted to 125 ml with virus dilution medium and added to confluent monolayers of Vero-E6 cells in twenty-five T175 flasks (Nunc; 5 ml/flask). After 1 hour of incubation at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... Total mRNA was extracted from 190 pMHC-tetramerpos purified (Supplementary Figure 1A) virus-specific T-cell populations(37) using magnetic beads (Dynabead mRNA DIRECT kit; Invitrogen, Thermo Fisher Scientific). Ten μl (~1μg ...
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bioRxiv - Microbiology 2024Quote: ... A 10 µL RT–qPCR reaction to identify the RNA enteric viruses of interest contained 2.5 µL of fast virus 1-step master mix (4X) (Life Technologies, Carlsbad, CA, USA), 500 nM of each specific primers ...
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bioRxiv - Microbiology 2024Quote: ... half of the virus supernatant after the concentration step was digested with amplification grade DNAse I (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA), for 10 minutes at 37 °C before DNA extraction to reduce host DNA contamination ...
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bioRxiv - Microbiology 2023Quote: ... Virus detection was performed by quantitative RT-qPCR on a QuantStudio® 5 Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA), according toconditions described in Guedes et al ...
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bioRxiv - Cancer Biology 2022Quote: ... 266-6 CAS9 cells were infected with FUW-TetO-KRASG12D-BleoR virus and cells were selected with 5 mg/ml zeocin (Invitrogen, San Diego, CA) for 10 days ...
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bioRxiv - Pathology 2023Quote: ... RNA was reverse-transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (Life Technologies, Carlsbad, CA, USA) on the LC96 instrument (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... Synthesis of c DNA from 1000 ng of pooled total RNA was carried out by using 10 μM of reverse primer from each reconstructed RNA virus and RevertAid Reverse Transcriptase (Thermo Fisher scientific, USA) (Cat ...
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bioRxiv - Microbiology 2023Quote: ... and real-time RT-PCR was performed to detect the presence of influenza A virus (M gene) using AgPath-ID™ One-Step RT-PCR Kit (Thermo Fisher Scientific). Positive influenza samples were then selected for whole genome sequencing using next generation sequencing (NGS ...
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bioRxiv - Neuroscience 2023Quote: ... The infusion line was primed with a loading line solution that was identical to the virus suspension solution [Corning phosphate-buffered saline without Ca2+ or Mg2+ with 350 mM NaCl (Gibco, ThermoFisher Scientific, Waltham, MA) containing 0.001% F68 surfactant (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA concentrations of 30 samples were normalized to approximately 15 ng µl-1 and TSV was detected and quantified from 2 µl of sample using a TaqMan™ Fast Virus 1-Step Master Mix (Applied Biosystems™) following the OIE recommended protocol and triplicate standards (mean R2 = 0.93 ± 0.06 ...
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bioRxiv - Microbiology 2023Quote: ... in a total volume of 25 µL containing 5 µL of template employing Taqman Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, USA). Oligonucleotide primer and probe sequences and thermocycling conditions are detailed in Table S2 ...
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bioRxiv - Bioengineering 2022Quote: ... vRNA levels were determined by quantitative real-time PCR (qRT-PCR) using Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix (Thermofisher #4444432) and a primer-probe combination recognizing a conserved region of gag (GAG5f ...
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bioRxiv - Synthetic Biology 2024Quote: MetBo2 (polyoma middle T oncogene–induced mouse mammary tumor on a syngeneic Friend Virus B NIH Jackson (FVB) background)[23] cells were maintained in 1X Dulbecco’s Modified Eagle Medium (DMEM) (Thermofisher Scientific; Cat. No. 11995065) with 10 % Fetal Bovine Serum (FBS ...
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bioRxiv - Systems Biology 2023Quote: ... and yield was determined by quantitative real-time PCR using the TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems, Foster City, CA) and TaqMan Gene Expression Assay ...
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bioRxiv - Immunology 2024Quote: ... lentiviral particles were generated through the transient transfection of plasmid into 293T virus- producing cells (ATCC, Manassas, VA, USA) in the presence of Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA), vesicular stomatitis virus G (VSV-G) ...
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bioRxiv - Immunology 2024Quote: ... RT-qPCR was used to detect and determine virus titers using the AgPath ID one-step RT-PCR kit (ThermoFisher Scientific, Waltham, MA) from the cell culture supernatants as previously described [28] ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was reverse-transcribed using Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (RT) according to the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific, cat# 28025013). For quantitative Real Time Polymerase Chain Reaction (RT-qPCR) ...
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bioRxiv - Immunology 2024Quote: ... and cDNA was reverse transcribed using the Moloney murine leukemia virus reverse transcriptase and random hexamer oligonucleotides for priming (Life Technologies, CA, USA). The expression of the genes HIF-1α and LDH-A was determined using the PCR SYBR Green sequence detection system (Eurogentec ...
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bioRxiv - Cancer Biology 2024Quote: One microgram of total RNA was reverse transcribed at 37□°C for 60□min in the presence of random hexamers and Moloney murine leukaemia virus reverse transcriptase (Invitrogen, Carlsbad, CA, USA). Specific oligonucleotide primers for
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bioRxiv - Microbiology 2022Quote: Recombinant trimeric ectodomain of SARS-CoV-2 S protein with two proline mutations (rtS-ecto2P) as a vaccine antigen was produced using a Drosophila expression system (Thermo Fisher Scientific, Grand Island, NY). The protein sequence was modified to remove the furin cleavage site (RRAR to GSAG) ...
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bioRxiv - Immunology 2020Quote: ... then stained with surface and/or intracellular flow cytometry antibodies specific to antigens of interest using eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, Waltham, MA) according to manufacturer protocol ...
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bioRxiv - Immunology 2024Quote: ... cells were stained following the ThermoFisher “BestProtocols: Staining Intracellular Antigens for Flow Cytometry – Protocol A” using the eBioscienceTM Permeabilization Buffer (ThermoFisher Scientific cat#: 00-8333-56) and eBioscienceTM IC Fixation Buffer (ThermoFisher Scientific cat# ...
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bioRxiv - Plant Biology 2024Quote: Coating: Prepared sample were added to the selected ELISA-suitable antigen-binding 96-well microtiter plate (CORNING COSTAR 9018; Thermofisher scientific Ref: 44-2504-21). The first two columns contained the peptide standard ...