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Citations for Thermo Fisher :
1051 - 1071 of 1071 citations for Canine Parainfluenza Virus Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... cells were stained following the ThermoFisher “BestProtocols: Staining Intracellular Antigens for Flow Cytometry – Protocol A” using the eBioscienceTM Permeabilization Buffer (ThermoFisher Scientific cat#: 00-8333-56) and eBioscienceTM IC Fixation Buffer (ThermoFisher Scientific cat# ...
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bioRxiv - Plant Biology 2019Quote: ... 2002) containing the 35S Cauliflower Mosaic virus promoter and eGFP gene using the Gateway® LR Clonase™ II kit (Invitrogen Corp., Carlsbad, CA). A single colony was used to amplify the [Gene-pK7FW2/pK7WGF2] plasmid and it was sequenced before transforming Agrobacterium (strain GV3101 ...
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bioRxiv - Microbiology 2019Quote: ... The number of JEV RNA copies present in BBB-crossing samples was determined by RT-qPCR using TaqMan® Fast Virus 1-Step Master Mix kit (Applied Biosystems®, 4444432) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... MHV-A59 was quantified by RT-qPCR by amplifying a fraction of its ORF5 gene for the structural protein M.15,16 The TaqMan™ Fast Virus 1-Step Master Mix Kit (Thermo Fisher Scientific Inc., 4444432) was used for RT-qPCR ...
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bioRxiv - Microbiology 2020Quote: ... and Mayaro virus (MAYV, TRVL 15537) were propagated in Aedes albopictus C6/36 cells grown in Minimum Essential Medium (MEM, Thermo Fisher Scientific, Waltham, MA). SARS-CoV-2 (SARS-CoV-2/CANADA/VIDO 01/2020 ...
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bioRxiv - Neuroscience 2021Quote: Images of whole brain slices for analysis of virus expression were obtained from a multichannel fluorescence microscope (EVOS FL Auto Imaging System, Thermo Fisher Scientific, Waltham, MA) with an automatic objective at 10X magnification ...
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bioRxiv - Microbiology 2021Quote: ... cDNA were synthesized from 100 pg of RNA and quantified using the TaqMan® Fast Virus 1-Step Master Mix (Thermofisher, Waltham, Massachusetts, USA) following manufacter’s instructions with appropriate oligonucleotides and Taqman probes (Table S1) ...
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bioRxiv - Immunology 2020Quote: Subgenomic RT-qPCR was performed on the QuantStudio™ 7 Flex Real-Time PCR System using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with forward primer ...
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bioRxiv - Immunology 2021Quote: The RBDwt protein was conjugated to Cucumber mosaic virus - tetanus toxoid (CuMVTT) using the linker Succinimidyl 6-(beta-maleimidopropionamido) hexanoate (SMPH) (Thermo Fisher Scientific, Waltham, USA). CuMVTT contains a powerful T cell epitope of the Tetanus toxin (TT ...
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bioRxiv - Microbiology 2022Quote: ... The gradient was collected into 500 μL fractions and the virus containing fractions were detected using NanoDrop 1000 Spectrophotometer (Thermo Scientific, Waltham, Massachusetts, US). The virus containing sucrose fractions were diluted at least 1:5 in 2 mM MgCl2-PBS and the virus was pelleted by centrifugation (70Ti rotor ...
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bioRxiv - Microbiology 2023Quote: Cell culture supernatants or P1 virus cultures were mixed with a five-fold excess of TRIzol™ LS Reagent (Thermo Fisher Scientific, Waltham, MA). Viral RNAs were extracted by Direct-Zol™ RNA miniprep Plus Kit (ZYMO RESEARCH ...
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bioRxiv - Microbiology 2023Quote: ... Isolated RNA was subjected to one-step RTqPCR analysis as described using primers specific for the viral genome encoding for the RNA-dependent RNA polymerase (RdRp)23 and for E24 genes with TaqMan fast virus 1-step master mix (ThermoFisher Scientific, catalogue number 4444432) using AriaMx instrumentation (Agilent ...
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bioRxiv - Neuroscience 2023Quote: ... The infusion line was primed with a loading line solution that was identical to the virus suspension solution [Corning phosphate-buffered saline without Ca2+ or Mg2+ with 350 mM NaCl (Gibco, ThermoFisher Scientific, Waltham, MA) containing 0.001% F68 surfactant (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Isolated RNA was directly subjected to one-step RT-qPCR analysis using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, MA, USA) and Bio-Rad CFX instrumentation (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Porcine reproductive and respiratory syndrome virus [PRRSV] European and North American strains (LSI VetMAX™ PRRSV EU/NA Real-Time PCR Kit; Thermo Fisher Scientific, MA, USA), Swine influenza virus [SIV] (EXOone Influenza A ...
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bioRxiv - Microbiology 2021Quote: Vero81 cells were adsorbed with CHIKV strains diluted in virus dilution buffer (VDB, RPMI medium with 25 mM HEPES [Gibco] supplemented to contain 1% FBS) at a multiplicity of infection of 0.01 plaque-forming units (PFU)/cell at 37°C for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... Pyrene-labeled virus was produced in BHK-21 cells cultured beforehand in the presence of 15 μg/ml 1-pyrenehexadecanoic acid (Thermo Fisher Scientific, Waltham, MA, USA). The supernatant was harvested at 48 hpi ...
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bioRxiv - Microbiology 2022Quote: ... plasmid encoding myc- and His-tagged full-length EBOV GP (Zaire ebolavirus isolate Ebola virus/H.sapiens-wt/SLE/2014/Makona-G3838) using Lipofectamine™ 3000 (Thermo Fisher Scientific Cat. Nr. L3000) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Infiltrated leaves were collected 5 days after infiltration and homogenised with virus inoculation buffer (10 mM monopotassium phosphate containing 1% Celite (Thermo Fisher Scientific, 68855-54-9)) ...
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bioRxiv - Microbiology 2022Quote: ... Clones expressing different surface antigens were obtained by limiting dilution in 96-well culture plates placed in anaerobiosis chambers (Anaerogen® Compact, Thermo Scientific® Oxoid®, Cat. # AN0010C) at 37 °C during 5 days ...
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bioRxiv - Immunology 2022Quote: ... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...