Labshake search
Citations for Thermo Fisher :
1001 - 1050 of 1123 citations for Canine Parainfluenza Virus Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... isolated RNA was run using a TaqMan Fast Virus One-Step Master Mix (applied Biosystems) on a QuantStudio 3 Flex Real-Time PCR system (Applied Biosystems). RNA standards with known copy numbers were used to generate a standard curve and calculate sample copy numbers ...
-
bioRxiv - Cancer Biology 2024Quote: ... Virus to construct stable HA-B56α overexpression cell lines were produced using HEK293T cells transfected using Lipofectamine 3000 (Fisher Scientific, L3000015) with the plasmid of interest (POI ...
-
bioRxiv - Neuroscience 2024Quote: ... The fluorescent protein expressed by the virus (either GFP or mCherry) was visualized using an EVOS FL tabletop fluorescent microscope (ThermoFisher Scientific). Images were taken for future reference ...
-
bioRxiv - Pathology 2021Quote: ... for 1 week and pepsin (1 mg/ml in 0.1N HCl) was used for antigen retrieval for immunostaining with human COMP (Thermofisher; MA1-20221, 1:100), CHOP (Santa Cruz ...
-
bioRxiv - Molecular Biology 2020Quote: ... a female human embryonic kidney cell line transformed with the SV40 large T antigen was obtained from ATCC (#CRL-1573) and cultured in complete media consisting of DMEM (#11965092, GIBCO, Life Technologies; Carlsbad, CA) supplemented with 10% FBS (Seradigm Premium Grade HI FBS ...
-
bioRxiv - Microbiology 2020Quote: ELISA was carried out to estimate the avidity and overall stability of antigen-antibody complexes using the chaotropic agent ammonium thiocyanate (NH4SCN) (Acros Organics Fisher Scientific). The plates were coated with 50 ng of PvRBP2b161-1454 in PBS and incubated overnight ...
-
bioRxiv - Biophysics 2022Quote: ... We revealed the antigens coating with a Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor 647, Invitrogen, ref. A-21447) and analyzed the fluorescence intensity ...
-
bioRxiv - Microbiology 2020Quote: Total RNA was extracted from cell pellets collected in QuantiFERON-TB gold assay tubes without antigen using the Ribopure Ambion RNA isolation kit (Life Technologies, California, USA), and eluted RNA treated with DNase for 30 min ...
-
bioRxiv - Neuroscience 2021Quote: ... and adjusted tissue and plasma extracts were added with the antiserum against OT (1:1,000 dilution) to each antigen-coated well of a 96-well microplate (F96 maxisorp nunc-immuno plate;Thermo Fisher Scientific, Roskilde, Denmark) and incubated overnight at 4°C ...
-
bioRxiv - Bioengineering 2023Quote: ... for antigen retrieval in a pressure cooker for 10 minutes then sections were blocked in CAS-block histochemical reagent (Thermo Fisher Scientific, 008120). Permeabilization was performed with 0.5% Triton X-100 in PBS for 5 minutes ...
-
bioRxiv - Neuroscience 2023Quote: ... by measuring the expression of pluripotency surface antigen (SSEA4 1:20, Invitrogen, 12-8843-42, and TRA1-60 1:20, Invitrogen, 12-8863-82) by flow cytometry ...
-
bioRxiv - Cancer Biology 2023Quote: ... Both FFPE and OCT cell array samples were incubated for 5min at 100°C in 1x Antigen Retrieval Solution (ThermoFisher, 00-4956-58) using a pressure cooker ...
-
bioRxiv - Pathology 2023Quote: Slides were incubated at 90°C for 20 minutes in High pH IHC Antigen Retrieval Solution (Thermo Fisher Scientific Catalog Number 00-4956) and allowed to cool to room temperature ...
-
bioRxiv - Microbiology 2021Quote: ... Viral RNA was measured by quantitative real real-time polymerase chain reaction (qRT-PCR) using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with primers and probe specific for the CCHFV L gene.
-
bioRxiv - Evolutionary Biology 2021Quote: ... we harvested virus from each virus line under sterile conditions by collecting up to 10 virus killed cadavers per line and transferring these to sterile 15 mL disposable tissue grinders (ThermoFisher Scientific, U.S.A.). Infected larvae were recognizable by their opaque ...
-
bioRxiv - Immunology 2021Quote: ... Total mRNA was extracted from 190 pMHC-tetramerpos purified (Supplementary Figure 1A) virus-specific T-cell populations(37) using magnetic beads (Dynabead mRNA DIRECT kit; Invitrogen, Thermo Fisher Scientific). Ten μl (~1μg ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... A common virus stock was generated by transfection of plasmid DNA into NLFK cell culture using Lipofectamine 2000 (Life Technologies, Carlsbad, CA) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2019Quote: ... ZHBTc4 cells were transduced with pLV-pCAGGS-Tir1-V5 and PLEX-mCherry-OCT4-AID packaged lentiviral vectors (i.e Tir1-V5 and mCherry-Oct4-AID virus, respectively) and were selected with 2 μg/ml Puromycin (Thermo Fisher Scientific #A1113803) for 10 days ...
-
bioRxiv - Microbiology 2020Quote: Proteins were harvested four to five days post virus infection in RIPA Lysis and Extraction Buffer (Boston Bio) with 1X Protease Inhibitor and 1X Phosphatase Inhibitor (Thermo Fisher Scientific). Protein Concentration was determined using BCA Assay Kit (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2019Quote: ... cells (in-house stock regularly tested to be virus- and mycoplasma-free) were grown at 25°C in S2 cell media supplemented with 10% fetal bovine serum (Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2021Quote: ... the extracellular RSV virus were collected by PEG6000 precipitation method and intracellular RSV virus were collected by lysing the macrophage cells with the lysis buffer from mirVanaTM miRNA Isolation Kit (Ambion, Life Technologies, AM1560). Total RNA from RSV infected macrophage cells as well as stock virus was extracted using mirVanaTM miRNA Isolation Kit according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... Purity and titer of virus were then assessed by SDS-PAGE and SYPRO ruby staining (S-12000, Life technologies, Carlsbad, CA, USA).
-
bioRxiv - Microbiology 2021Quote: ... For the preparation of the BGLF2-KO virus, HEK293/EBV(dBGLF2) cells (Konishi et al., 2018) were transfected with BZLF1 expression plasmid using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... and quantitative real-time polymerase chain reaction (qRT-PCR) was conducted using TaqMan Fast Virus 1-Step Master Mix (Thermofisher Scientific, US) with primers and probe specific for the SARS-CoV-2 E gene following guidelines by the World Health Organization (https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assayv1991527e5122341d99287a1b17c111902.pdf ...
-
bioRxiv - Microbiology 2021Quote: ... HCV RNA was quantified by one-step reverse transcription-quantitative PCR using 50 ng of total intracellular RNA and TaqMan® Fast Virus 1-Step Master Mix (Applied Biosystems) with primers and probe targeting the HCV 5’ nontranslated region as described previously [82] ...
-
bioRxiv - Microbiology 2022Quote: The ZEBOV RT-PCR assay was performed in a 25 µL reaction mixture containing TaqMan Fast Virus 1-step master mix (Thermo Fisher Scientific), 5 µl template RNA ...
-
bioRxiv - Microbiology 2019Quote: ... total RNA was reverse transcribed using virus-specific RT primers or oligo-dT primers and the SuperScript III First-Strand Synthesis System (Invitrogen, Carlsbad, CA). Real-time quantitative PCR was performed on the Applied Biosystems 7500 system with QuantiTect SYBR Green PCR Master Mix (Qiagen ...
-
bioRxiv - Neuroscience 2019Quote: cDNA sequences from the leader through the first half of the rabies virus P gene were amplified using Platinum SuperFi Green Master Mix (Invitrogen (Thermo Fisher), USA ...
-
bioRxiv - Microbiology 2020Quote: ... apical poles were gently washed with warm PBS and then infected with a 150-μL dilution of HRSV-A (Long) virus in OptiMEM medium (Gibco, ThermoFisher Scientific) at a MOI of 1 ...
-
bioRxiv - Microbiology 2021Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
-
bioRxiv - Physiology 2020Quote: ... One microgram of RNA was reverse transcribed into cDNA using oligo-dT and MMLV-RT (Moloney Murine Leukemia Virus Reverse Transcriptase, Invitrogen, Thermo Fisher Scientific). Before cDNA synthesis ...
-
bioRxiv - Neuroscience 2019Quote: ... Patient KS1-1 was consented for stem cell derivation and cells were reprogrammed using non-integrating Sendai virus vectors (CytoTune-iPS 2.0, ThermoFisher Scientific, Waltham, MA). 5 days post-induction ...
-
Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: Viral RNA for real-time PCR assay was extracted from 140 ml of virus isolate (previously tittered as described above) and serum samples using Invitrogen PureLink RNA Mini Kit (Invitrogen, Valencia, CA) as per the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Cell supernatants were harvested at 48 h post-infection and virus titers were determined by infecting TZM-bl cells and measuring β-galactosidase activity using Galacto-Lite system (Applied Biosystems). For flow cytometry quantification of infection ...
-
bioRxiv - Immunology 2021Quote: ... T2 target cells and Epstein-Barr Virus-transformed B lymphoblastoid cell lines (EBV-LCLs) cells were cultured in RPMI-1640 (Thermo Fisher Scientific) supplemented with 10% FCS and 1% penicillin/streptomycin (#P433 ...
-
bioRxiv - Immunology 2020Quote: ... at a concentration to give 100 to 250 foci per well in the virus only control wells was mixed 50:50 in 1% FCS MEM with 1 x antibiotic/antimycotic (Gibco, 15240-062) with serum doubling dilutions from 1:20 to 1:640 (or higher dependent on antibody levels ...
-
bioRxiv - Immunology 2020Quote: ... Virus heat inactivation at 56 °C for 30 minutes and total RNA including virus RNA extraction was performed using TRIzol™ Plus RNA Purification Kit (ThermoFisher, USA). cDNA synthesis was performed using GoScript™ Reverse Transcription System (Promega ...
-
bioRxiv - Immunology 2020Quote: ... pEco envelope was used to produce virus to infect murine T cells) using lipofectamine 2000 (Cat. No. 11668019, Invitrogen, Carlsbad, California, USA) on a 100□mm2 poly-D-lysine–coated plate (Corning ...
-
bioRxiv - Microbiology 2021Quote: ... RNA extracted from animal samples or RNA standards were then measured using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, catalog # 4444432) and custom primers/probes targeting the E gene sgRNA (forward primer ...
-
bioRxiv - Microbiology 2021Quote: ... Equal amounts of the cell lysate protein and equal volume of the virus pellet protein were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris gel; Invitrogen/Thermo Scientific) and transferred onto nitrocellulose membrane by semidry transfer ...
-
bioRxiv - Genetics 2022Quote: ... 30 μg of the virus) was digested with 1:100 (w/w) ratios of trypsin (Pierce Trypsin Protease mass spectrometry grade; Thermo Fisher Scientific)/virus for various time points at 25°C (31 ...
-
bioRxiv - Evolutionary Biology 2022Quote: Virus RNA from nasal swabs was extracted from 50 µL of supernatant using the MagMAX-96 AI/ND Virus RNA Isolation Kit (Ambion, Austin, TX) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2022Quote: ... Reverse transcription (RT) of the extracted RNA samples (1 µg) was performed using AMV (Avian Myeloblastosis Virus) Reverse Transcription Kit (Invitrogen, Waltham, USA) and oligo-dT18 (1 µM ...
-
bioRxiv - Microbiology 2023Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... The first-strand cDNA was obtained using oligo(dT) primers and Moloney murine leukemia virus-reverse transcriptase (MMLV-RT kit; Life Technologies, USA), using 100 ng of purified RNA ...
-
bioRxiv - Microbiology 2023Quote: ... the first-strand human cDNA was obtained using oligo(dT) primers and Moloney murine leukemia virus-reverse transcriptase (MMLV-RT kit; Life Technologies, USA), and The SuperScript™ VILO™ cDNA Synthesis Kit ...
-
bioRxiv - Cancer Biology 2023Quote: ... The virus supernatant was collected after 24 hrs and 48 hrs and filtered with a 0.45-mm syringe filter (Thermo Fisher, CA, USA). pLenti-shCtrl (negative silencing control ...
-
bioRxiv - Neuroscience 2023Quote: ... from the Stanford Gene Vector and Virus core unless otherwise specified) and Ctb retrograde tracers (1 ug/ul; Invitrogen #C34777, #C34776, #C34775) were infused with a syringe pump (Harvard Apparatus ...
-
Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... One μg DNA or 4 μL cDNA was used to PCR-amplify virus barcode (VBC) using Platinum SuperFi II DNA Polymerase (12361010, Invitrogen, Waltham, MA). Information on primer sequence including frameshifting nucleotide ...
-
bioRxiv - Microbiology 2023Quote: ... The pooled libraries were mixed with virus-specific 161 biotinylated probes in the presence of human Cot-1 DNA (Invitrogen, Cat#15279011) and xGen Universal Blocking Oligos (IDT ...