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Citations for Thermo Fisher :
1051 - 1100 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... 1 mg/mL in 2:1 chloroform:methanol) is from Invitrogen (USA) and naphthopyrene from Sigma-Aldrich (St ...
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bioRxiv - Bioengineering 2020Quote: ... for 1 h at RT followed by Alexa Fluor 568-conjugated secondary antibodies (1:200 dilution, Invitrogen) for 45 min at RT ...
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bioRxiv - Immunology 2020Quote: ... Alexa-Fluor 555 conjugate secondary antibodies (Thermo Fisher Scientific, USA, 1:400, 1 h at 37°C) were used for labelling Spike ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation for 1 h with goat anti-rabbit AF647 (1:400; A-21224, Molecular Probes) or goat anti-mouse AF488 (1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... humidified chamber for 1 h with anti-mouse coupled to Alexa Fluor 488 (1:1000, Invitrogen, A11001), anti-rabbit coupled to Alexa Fluor 555 (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibody anti-rabbit A555 was incubated at room temperature for 1 h (1:500, Life Technologies). Nuclei were stained using DAPI (Sigma) ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by 1 h blocking and overnight incubation in either anti-chicken Alexa 488 (1:500, Invitrogen) or Cy5 anti-rabbit (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... osteoclasts were incubated for 1 h with Alexa Fluor 647-labelled phalloidin (1/1000, Life Technologies, #A22287), mouse anti-lys40 acetylated alpha tubulin (1/500 ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were washed three times in PBS and incubated in secondary antibody (2 mg/mL Alexa Fluor 488, Invitrogen, 1:1500, 2 mg/mL Alexa Fluor 594, Invitrogen, 1:1500; 2 mg/mL Alexa Fluor 568, Invitrogen, 1:1000) diluted in PBS for 1.5 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then resuspended in aCSF and incubated with DAPI (1:1000 dilution of 1mg/mL DAPI, Thermo Fisher, #62248 or 7-AAD 7-Aminoactinomycin D, A1310, Thermo Fisher) for FACS sorting ...
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Spatial rearrangement of the Streptomyces venezuelae linear chromosome during sporogenic developmentbioRxiv - Microbiology 2020Quote: ... The nucleoids were subsequently stained for 5 min at room temperature with 7-amino-actinomycin D (1 mg/ml 7-AAD in DMSO, Thermo Fisher Scientific) diluted 1:400 in PBS buffer ...
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bioRxiv - Microbiology 2023Quote: ... The qPCR reactions for the data corresponding to Figure 7 and Figure 7 – figure supplement 1 were carried out in 384-well plates on a QuantStudio™ 5 (Applied Biosystems). The thermal cycling conditions were as follows ...
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bioRxiv - Molecular Biology 2022Quote: ... 4ºC) and incubated with 50 μL of 5 μM DAF-FM (4-amino-5methylamino-2’,7’-dichlorofluorescein diacetate, Life Technologies, Eugene, OR, USA) diluted in 1X PBS for 30 min at 34 °C ...
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bioRxiv - Biochemistry 2022Quote: ... After a clarifying spin for 15 min at 14,000 ×g and 4°C 2 μl (MICOS complex) or 7 μl (all other mitochondrial protein complexes) G-250 Sample Additive (Invitrogen, Waltham, Massachusetts, USA) containing 40% glycerol were added ...
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bioRxiv - Microbiology 2020Quote: ... we used 15 μL of a mixture of 4’,6-diamidin-2-phenylindole (4 μg mL−1) in SlowFade Gold Antifade Mounting medium (both Thermo Fisher Scientific, Waltham, MA, USA). All solutions and buffers for virus-targeted genomeFISH experiments were prepared with molecular grade water (Carl Roth ...
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bioRxiv - Genetics 2022Quote: ... 2% B-27 and 1% N-2 supplements (Life Technologies) and supplements 20ng/ml BDNF ...
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bioRxiv - Microbiology 2022Quote: ... 2% B-27 and 1% of N-2 supplements (GIBCO), 1% L-glutamine ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2 μg mL−1 puromycin (58-58-2, ≥98%, Invitrogen) and 100 μg mL−1 hygromycin (31282-04-9 ...
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bioRxiv - Neuroscience 2024Quote: ... 2% B-27 and 1% of N-2 supplements (GIBCO), 1% L-glutamine ...
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bioRxiv - Microbiology 2020Quote: ... The purified linearized vector and digested foxA gene were ligated (1:3 molar ratio) overnight at 4°C using T4 DNA ligase (Thermo Scientific). The construct was transformed into competent E ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then rinsed 4 x 15 min in PBS using the nuclear counterstain TO-PRO-3 Iodide (1:2000, Thermo Fisher). For preservation of immunofluorescence ...
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bioRxiv - Bioengineering 2020Quote: ... We performed ERBB2 SNAPD on a 9:1 mixture of MOLT-4 and SK-BR-3 cells stained with CellTrace™ Calcein Red-Orange AM (Invitrogen). We then reinjected these droplets onto a dielectric sorting device (Fig ...
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bioRxiv - Neuroscience 2022Quote: ... CAT-1 (encoded by SLC7A1) and CAT-3 (encoded by SLC7A3) had 4 substrates including L-Lysine hydrochloride (Fisher Scientific #BP386), L-Histidine (Sigma-Aldrich #H6034) ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were incubated for 20 min at 37°C in PBS containing 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16; 1 μM; Thermo Fisher Scientific). To determine neutral lipid content ...
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin was stained with To-Pro-3 (1/200 dilution, 1 mM, Invitrogen T3605).
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bioRxiv - Neuroscience 2022Quote: ... dried and coverslipped under glycerol:TBS (3:1) with Hoechst 33342 (1:1000; ThermoFisher Scientific). Primary antibodies with the following dilutions used in this study include ...
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bioRxiv - Pathology 2024Quote: ... and 3-hydroxybutyrate dehydrogenase 1 (15417-1-AP, Thermo Fisher Sci, Waltham, MA, USA). HRP-conjugated secondary antibodies (NA9340 ...
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bioRxiv - Biochemistry 2024Quote: ... columns were washed with 3 column volumes (CV) of 1:1 RNase ZAP (Ambion) followed by 3 CV nuclease-free water (DEPC-treated MilliQ water) ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining 50 μL were incubated at 4 °C for 2 h with 40 μg of HisPur Ni–NTA magnetic beads (Thermo Scientific) pre-equilibrated in binding buffer ...
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bioRxiv - Immunology 2021Quote: ... was incubated with 20 μg of NKp46-IgG1Fc or NKG2D-IgG1Fc fusion protein at 4° C with rotary agitation for 16 h and then incubated with 2 mM of DTSSP crosslinker (ThermoFisher Scientific) for 2 h on ice ...
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bioRxiv - Bioengineering 2021Quote: ... Medium containing lipid complexes was removed 4 h post transfection and replaced with exosome-depleted phenol red-free DMEM + 10% FBS + 2 mM L-glutamine (Thermo Fisher). Cells were incubated with 100 nM TO-1 in HBSS starting 30 minutes prior to imaging and for the duration of the imaging period ...
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bioRxiv - Neuroscience 2020Quote: ... at 4 ng/μL and then pulled-down for 2 h using protein G-coupled dynabeads (50 μL, Thermo Fisher Scientific). After washing 3 times with 0.3 M NaCl solution ...
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bioRxiv - Systems Biology 2022Quote: ... equal amounts of protein lysates were incubated with pull-down antibody overnight at 4°C followed by 2 h treatment with Dynabeads Protein G magnetic beads (10004D, ThermoFisher, MA) for pull down ...
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bioRxiv - Pathology 2021Quote: ... Equal amounts of GST-tagged TaBln1 or GST (negative control) were mixed with TaCam3-His and incubated at 4°C for 2 h with GST beads (Thermo Scientific). The beads were collected and washed five times using the GST Protein Interaction Pull down Kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The cleared cell lysate was incubated for 2 h at 4 °C in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Thermo Scientific, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and loaded for 2 h at 4°C on a bi-directional rotator with 2 mM Ca2+ medium containing drugs 30 μM pancuronium (Fisher Scientific), or 38.9 μM curare (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining 50 μL were incubated at 4°C for 2 h with 40 μg of pre-equilibrated HisPur Ni–NTA magnetic beads (Thermo Scientific) in a final volume of 200 μL ...
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bioRxiv - Genomics 2020Quote: ... or a positive control probe 5’-5Alexa488N/(ATA)8TUU (ATA)7-3’ (Invitrogen). Reactions were incubated in a water bath at 37°C for 2 hrs ...
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bioRxiv - Immunology 2020Quote: ... Apoptosis was detected using the CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher). Frames were captured over a period of 24 hrs at 1 hour intervals from 4 separate 1.75 x 1.29 mm2 regions per well with a 10× objective using IncuCyte S3 live-cell analysis system (Sartorius) ...
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bioRxiv - Immunology 2020Quote: ... Apoptosis was detected using the CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher). Frames were captured over a period of 24hrs at 1-hour intervals from 4 separate 1.75 x 1.29 mm2 regions per well with a 10× objective using IncuCyte S3 live-cell analysis system (Sartorius) ...
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bioRxiv - Immunology 2021Quote: ... In vitro derived PCs were stained with CellEvent Caspase 3/7-Green (ThermoFisher), tetrametylrhodamine ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with CellEvent Caspase 3/7 Detection Reagent (ThermoFisher Scientific, cat. no. C10423) to a final concentration of 2 μM ...
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bioRxiv - Immunology 2021Quote: ... and 8 μM of CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen) for 30 min at 37°C 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cytotoxicity was assessed using the CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) at 1.0 µM and gating on CD45-negative cells ...
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bioRxiv - Cell Biology 2020Quote: ... for 4 h in DMEM containing dialyzed FBS (Gibco A3382001). The labeled cells were washed with ice-cold PBS and lysed on ice in Co-IP buffer supplemented with 10 mM MgCl2 ...
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bioRxiv - Cell Biology 2024Quote: ... The plate was blocked with 1% BSA in PBS for 1 h and then incubated with anti-MTCO2 (1:200 dilution, clone 12C4F12, Thermo Fisher Scientific) or anti-Cox6 (1:200 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed 3×5 min in PBS and incubated for 2 h with an Alexa Fluor® Plus 555-conjugated secondary antibody (Invitrogen A32816) diluted in the blocking solution ...
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bioRxiv - Immunology 2021Quote: ... 1 μg/ml -CD28 and 1:50 diluted conditioned supernatant from COS-7 cells transfected with a pcDNA1 vector (Invitrogen) encoding for recombinant chicken IL-2 (a kind gift from prof ...
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bioRxiv - Immunology 2022Quote: ... Treg cells were stimulated for 7-8 days with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... with 4 μg of a plasmid mixture (1:1:4 THR:RXR:TRE-Luc) in 200 μL Opti-MEM (Invitrogen, 11058-021) and added to the cells ...