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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2019Quote: ... sections were incubated 1 h with secondary antibodies (Alexa Fluor, Invitrogen). Slides were finally mounted in Fluoromont (Southern Biotech) ...
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bioRxiv - Plant Biology 2019Quote: ... followed by immunoprecipitation for 1 h with Dynabeads Protein G (Invitrogen). The precipitated RNA was extracted with Trizol reagent and used for RT-qPCR analysis.
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bioRxiv - Cell Biology 2021Quote: ... or IgG (H#L) specific secondary antibodies (Life Technologies, 1:500) and DAB (3,3’-Diaminobenzidine ...
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bioRxiv - Neuroscience 2021Quote: ... Goat anti-Rabbit IgG (H+L) HRP (1:2000; Invitrogen, 32460). Staining ...
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bioRxiv - Biophysics 2022Quote: ... followed by 1 h in 5% acetic acid (Fisher Scientific, 10171460), and then washed thoroughly in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL of Maxima H Minus Reverse Transcriptase (Life Technologies, EP0751) was added to the reaction mixture ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 h and mounted in ProLong Diamond Antifade Mountant (Invitrogen). Images were collected using ×40 oil immersion objective on an FV3000 confocal microscope (Olympus ...
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bioRxiv - Microbiology 2022Quote: ... (H+L) Alexa Fluor® 594 conjugate (1:500, Invitrogen, A11032). Labeled secondary antibody incubation was performed for 1 hour at room temperature after which the slides were mounted using Fluoromount-G Mounting Medium with DAPI counterstain (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... blocked for 1 h with 10% fetal bovine serum (FBS; Gibco) in PBS ...
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bioRxiv - Plant Biology 2023Quote: ... polyclonal Goat anti-rabbit IgG (H+L)/HRP (1:5000; ThermoFisher) for 1 hr ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked for 1 h in I-BlockTM (Applied Biosystems) protein-based blocking reagent and then incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibody staining was performed for 1 h in PBS (Gibco), followed by washes and secondary antibody staining for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Goat anti-chicken (IgY H+L) A488 (1:500, Invitrogen, #A11039), Goat anti-rabbit (IgG H+L ...
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bioRxiv - Neuroscience 2023Quote: ... Goat anti-rabbit (IgG H+L) A635 (1:500, Invitrogen, A31576).
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bioRxiv - Neuroscience 2023Quote: ... Goat anti-Rabbit (IgG H+L) A488 (1:500, Invitrogen, #A11008), Goat anti-chicken (IgY H+L ...
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bioRxiv - Neuroscience 2023Quote: ... Goat anti-rabbit (IgG H+L) A546 (1:500, Invitrogen, #A11010), Goat anti-rabbit (IgG H+L ...
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bioRxiv - Plant Biology 2024Quote: ... run for 1 h at 190 V in Novex Nupage (Invitrogen) and stained by the Silver staining method.
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bioRxiv - Developmental Biology 2020Quote: ... the ovaries were incubated for 2 h at room temperature with secondary Alexa 568-conjugated antirabbit IgG (1:500 in PBX; Invitrogen, Life Technologies), washed and counterstained with Hoescht (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 3 times with 3X PBS for 10 min/each and 1 time with PBS and incubated for 2 h at room temperature with secondary antibodies - Alexa Fluor 555 donkey anti-sheep at 1:1000 dilution (Invitrogen, cat# A21436), Alexa Fluor 647 donkey anti-mouse at 1:200 dilution (Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... primary antisera incubated sections were washed three times in PBS each for 10 minutes and subsequently incubated for 2 hours at room temperature in PBS with 2% NGS plus a dilution of an Alexa Fluor 488 goat anti-rabbit IgG (H+L) (1:1000, Molecular Probes, #A11034) or Alexa Fluor 594 goat anti-mouse IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 h for cells or 2 h for tissues at room temperature and the nuclei were stained with Draq5 (1:1000, Thermo Fisher Scientific) for 40 min or 4′,6- diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2 h incubation with secondary antibody goat-anti-rabbit Alexa Fluor 488 (1:500, #A11008, Invitrogen-Thermo Fisher, MA, USA). Sections were transferred to glass slides and covered with Fluoromount (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2 h incubation with secondary antibody goat-anti-rabbit Alexa Fluor 488 (1:500, #A11008, Invitrogen-Thermo Fisher, MA, USA). Sections were transferred to glass slides and covered with Fluoromount (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibody incubation was followed by 2 h incubation at room temperature with corresponding fluorescent secondary antibodies (1:500, Thermo Fisher Scientific). Nuclei were counterstained with DAPI (1:2000 in 0.1 M PB ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed to remove the excess of primary antibody and incubated with the appropriate Alexa-Fluor secondary antibodies for 2 h at 25 °C (1:1000, Thermo Fisher Scientific). The excess of secondary antibody was washed ...
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bioRxiv - Immunology 2022Quote: ... with PBS-T followed by a 2 h incubation on a shaker at room temperature with goat anti-rabbit IgG-HRP (1:5000) (Thermo Fisher Scientific) in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2 h incubation with secondary antibody goat-anti-rabbit Alexa Fluor 488 (1:500, #A11008, Invitrogen-Thermo Fisher, MA, USA). Sections were transferred to glass slides and covered with Fluoromount (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2 h incubation with secondary antibody goat-anti-rabbit Alexa Fluor 488 (1:500, #A11008, Invitrogen-Thermo Fisher, MA, USA). Sections were transferred to glass slides and covered with Fluoromount (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed in PB (3 × 10 min) and incubated for 2 h at room temperature with a secondary Alexa Fluor 488 donkey anti-goat antibody (Invitrogen, 1:500). Sections were rinsed in PB again (3 × 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h with the appropriate secondary antibody (AlexaFluor™ 488 goat anti-rabbit IgG; 1:1,000; Molecular Probes, USA). Sections were then washed in TBS 0.05M ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated for 2 h in blocking/permeabilization buffer including goat anti-rabbit AlexaFluor 568 or goat anti-mouse 488 (Invitrogen; 1:500). Sections were washed in 0.01 M PBS after primary and secondary antibody incubations ...
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bioRxiv - Cell Biology 2020Quote: ... organoids (after 1 week of culture) were incubated (37°C, 5% CO2, 1 h) with 10 µM EdU (5-ethynyl-2’-deoxyuridine, Thermo Fisher Scientific), a nucleoside analog of thymidine incorporated into DNA during active DNA synthesis ...
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bioRxiv - Genetics 2021Quote: ... 1 µg of intact RNA (with a 28S:18S rRNA ratio = 2:1) was reverse transcribed with the RevertAid H Minus Reverse Transcriptase kit (Thermo Scientific, EP0451). Real-time PCR reactions were performed with the Brilliant II SYBR® Green QPCR Master Mix (Agilent ...
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bioRxiv - Neuroscience 2021Quote: ... The specimens were blocked and permeabilized using permeabilization buffer for 2 h and incubated with rabbit polyclonal antibody anti-eNOS (1:200; PA1-037; Thermo Fisher Scientific) and goat polyclonal antibody anti–collagen IV (1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... for 2 h to stain all genomic DNA and subsequently with donkey anti-mouse Alexa Fluor 647 (Thermo Fisher, A31571; 1:25)) for 45 min ...
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bioRxiv - Biochemistry 2022Quote: ... PCR reaction mixture was loaded on 1% agarose gel (140 V for 2 h) and post-stained with SYBR gold (Thermo Fisher Scientific). The product bend was cut and DNA was extracted from the gel by Monarch DNA Gel extraction Kit (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed with PBS three times and then incubated for 2 h with 1:1000 goat anti-mouse Alexa 488 (Catalogue no. A11029, Invitrogen, CA, USA) and 1:1000 goat anti- rabbit Alexa 59 (Catalogue no ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 h followed by incubation with rabbit anti-goat IgG conjugated with Q-dot®655 (1:350 dilution; Life Technologies) for 90 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were incubated for 2 h at room temperature with secondary antibody Alexa 488 goat anti-rabbit IgG or rhodamine-phalloidin (1:500, Invitrogen, Molecular Probes). Samples were washed three times in 0.5% PBS-T and mounted in Vectashield containing DAPI.
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bioRxiv - Bioengineering 2022Quote: ... Incubation with secondary antibodies diluted in blocking solution was performed for 2 h at RT: Anti-mouse-Alexa 647 (1:500, cat. A32787, ThermoFisher Scientific Inc.), Alexa Fluor 488-Phalloidin (1:500 ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were then immunolabeled with Alexa Fluor 647-conjugated donkey anti-mouse IgG (Yeasen, Shanghai) in PBS with 1:400 dilution for 2 h and the nuclear counterstain DAPI (Thermo Fisher Scientific) for 5 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed in PBS and incubated for 2 h at room temperature with a goat anti-chicken Alexa 488 secondary antibody (#A11039, 1/500, Invitrogen, MA, US) and goat anti-mouse Alexa 555 (#A21422 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were washed with PBS to remove unbound antibody and incubated for 2 h with Alexa Fluor-conjugated secondary antibodies (Invitrogen; 1:500), 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2024Quote: ... 1:400 and for 1 hour at room temperature with the quaternary antibody: F(ab)’2-goat anti-rabbit IgG (H+L) A488 (Invitrogen, A-11070) 1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... in blocking buffer followed by PBS wash 15 min three times and then incubation for 2 h at room temperature with Alexa Fluor 594 conjugated donkey anti-rabbit antibody (1:1000, ThermoFisher A-21207). Stained samples were then washed for 15 min three times in PBS ...
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bioRxiv - Genomics 2022Quote: ... the purified gDNA was incubated at 37 °C for 1 h with RNase H (Thermo Fisher Scientific: 18021014) to remove hybridized RNA for RNase H-treated DRIP-seq library ...
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bioRxiv - Immunology 2020Quote: ... incubated for 1 h with secondary antibody Alexa Fluor 555 goat anti-mouse IgG (H+L) (A21422, ThermoFisher), washed with PBS ...
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bioRxiv - Genomics 2023Quote: ... All nuclei were pooled and stained with DAPI (4′,6-diamidino-2-phenylindole, 3 uM final) (Invitrogen, D1306). Using a FACSAria III cell sorter (BD Biosciences) ...
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bioRxiv - Biochemistry 2023Quote: ... cells were marked with 2,3x10-3 µg/µL 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific) for 10 min at RT in the dark ...
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bioRxiv - Cell Biology 2024Quote: ... and a lentiviral transfer plasmid (2:3:4 ratio by mass) using Lipofectamine 3000 (Thermo Fisher Scientific L3000015). Viral supernatant was harvested 48h after transfection and filtered through 0.45 µm cellulose acetate filters (Corning 431220) ...