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Citations for Thermo Fisher :
1051 - 1100 of 10000+ citations for 3 Thiophen 3 yl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated in 0.05 M Tris-HCl buffer (pH 7.6) containing 5 mg 3-3′ diaminobenzidine (Thermo Scientific, TA-060-HDX) per 10 mL buffer and a final concentration of 0.01% hydrogen peroxide for 15 min at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16C in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3). All embryos were raised in 0.22 μm filtered sea water (FSW ...
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bioRxiv - Immunology 2022Quote: ... targeting SAMHD1 (sense RNA 5’-GCAGAUAAGUGAACGAGAUTT-3’, antisense RNA 5’-AUCUCGUUCACUUAUCUGCAG-3’) or the negative control #1 siRNA using RNAiMAX (ThermoFisher, 13778-075). 24 h after transfection ...
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bioRxiv - Immunology 2022Quote: ... and hybridized first using RT primer (5′/5Biosg/AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3′) and then reverse transcribed into cDNA using TSO primer (5′-AAGCAGTGGTATCAACGCAGAGTACATrGrGrG-3’) and RT maxima reverse transcription (Thermo Fisher Scientific). cDNA was amplified using ISPCR primer (5′-AAGCAGTGGTATCAACGCAGAGT-3′ ...
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bioRxiv - Microbiology 2022Quote: ... The 16S rRNA gene was amplified with the primers 8f (5′-AGAGTTTGATCCTGGCTCAG-3′; Weisburg et al. 1991) and 1520 r (5′-AAGGAGGTGATCCAGCCGCA-3′; Edwards et al. 1989) (Invitrogen, CA, USA). A volume of 0.5 μL DNA extract was used for 50 μL PCR reactions containing 2 units Taq DNA polymerase ...
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bioRxiv - Molecular Biology 2023Quote: ... The following day the miR-423-5p or miR-Ctrl vector was cotransfected with either the Cacna2d2-3’UTR or the Cacna2d2 mut 3’UTR reporter vectors using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from 6 pairs of P21 mouse testes (3 pairs of wild type and 3 pairs of Mov10-/-) using TRIzol reagents (Thermo Fisher Scientific). 1 μg of total RNA from each sample was used to generate RNA-seq libraries using TruSeq Stranded mRNA Library Preparation Kit Set A (Cat ...
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bioRxiv - Biochemistry 2023Quote: The ibpA 5’ UTR-gfp mRNA produced from CUGA®7 in vitro transcription kit (Nippon Gene) was attached to a 3’-terminal biotinylated nucleotide using Pierce™ RNA 3’ End Biotinylation kit (Thermo Scientific). The biotin-labeled mRNA (0.1 µM ...
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bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
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bioRxiv - Physiology 2023Quote: ... Digitonin solubilized mitochondria were loaded 3-12% Bis-Tris Invitrogen™ Novex™ NativePAGE™ 3-12% acrylamide gel (1mm) (Invitrogen, BN2011BX10) using the anode running buffer (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Four microliters of LotA7-544 was loaded onto a glow-discharged grid and blotted for 3 s with blot force 3 using Vitrobot Mark IV (Thermo Fisher, U.S.A.).
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bioRxiv - Plant Biology 2024Quote: ... The purified RNA was reverse transcribed using the CLIP-3 PCR primer (5’-GTCTCGTGGGCTCGG-3’) and SuperScript IV reverse transcriptase (18090010; Thermo Fisher Scientific). The first-strand cDNA was then subjected to PCR using the CLIP-5 PCR primer (5’-TCGTCGGGCAGCGTC-3’) ...
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bioRxiv - Microbiology 2024Quote: Imaging of intracellular calcium levels and distribution was performed by Rhod 3 staining using the Rhod-3 Calcium Imaging Kit (Thermo Fisher Scientific) following the manufacturer’s protocol as described previously 14 ...
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bioRxiv - Neuroscience 2024Quote: ... 10 µg of Pantr1 sense RNA were used to label the 3’ end using Pierce™ RNA 3’ End Desthiobiotinylation Kit (Thermo Scientific). After labelling ...
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bioRxiv - Immunology 2024Quote: ... and activated by adding 50 mg/ml sulfo-NHS (N-hydroxysulfosuccinimide) and EDC [1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride] (Thermo Fisher Scientific). Each bead region was then incubated with 6.25 μg of the respective antigen yielding a final concentration of 6.25μg antigen/1 × 10^6 beads in coupling buffer [0.5 M 2-(N-morpholino ...
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bioRxiv - Microbiology 2024Quote: ... Serum samples were assayed at 3-fold dilutions starting at a 1:3 dilution in Blocker Casein in PBS (Thermo Fisher Scientific) diluent ...
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bioRxiv - Microbiology 2024Quote: ... total RNA was harvested from a 10 ml parasite culture (5% hematocrit; >3% parasitaemia) by resuspending the pelleted RBCs in 3 ml pre-warmed Trizol (Invitrogen #15596-018). After a 5 min incubation step at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Expression of Kdm5c was detected us-ing the primers 5’-CCCATGGAGGCCAGAGAATAAG-3’ 5’-CTCAGCGGATAAGAGAATTTGCTAC-3’ and nor-malized to TBP using the primers 5’-TTCAGAGGATGCTCTAGGGAAGA-3’ 5’-CTGTGGAGTAAGTCCTGTGCC-3’ with the Power SYBR™ Green PCR Master Mix (ThermoFisher #4367659).
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bioRxiv - Genomics 2024Quote: ... liver and muscle tissues from a total of 72 fish (n = 8 salmonid species x 3 biological replicates x 3 tissues) using TRIzol (Invitrogen/Life Technologies) with glass mill beads (1mm ...
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bioRxiv - Cancer Biology 2024Quote: ... sequence is 5’-GCCGACCAAUUCACGGCCG-3’ and siRNA directed against GNA11 (siGNA11) is 5’-AAAGGGUACUCGAUGAUGC-3’) using Lipofectamine™ RNAiMAX (13778150, Fisher Scientific) and harvested 48 h post-transfection.
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bioRxiv - Developmental Biology 2024Quote: ... followed by transfer to a positively charged nylon membrane and then crosslinked with EDC (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide) at 60°C for 1–2 hours and prehybridized with ULTRAhyb Ultrasensitive Hybridization Buffer (Invitrogen, cat # AM8670). The membrane was then hybridized with 50 pmol mL−1 Biotin-labeled Locked Nucleic Acid-modified DNA probes (designed and synthesized by Qiagen ...
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bioRxiv - Physiology 2022Quote: ... The samples were then mixed with 1 mL of a 3:13 solution of Ehrlich’s reagent (1.5 g of 4-[dimethylamino] benzaldehyde [ThermoFisher]; 5 mL ethanol; 337 µL sulfuric acid) to isopropanol and incubated for 30 min at 58°C ...
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bioRxiv - Plant Biology 2020Quote: ... on a QuantStudio 3 real-time PCR instrument (ThermoFisher). Values were normalized to the housekeeping gene SAND (At2g28390) ...
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bioRxiv - Biophysics 2021Quote: ... 3-(N-maleimidylpropionyl)biocytin (MBP) was obtained from Invitrogen and the HRP-conjugated antibody from eBioscience ...
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bioRxiv - Cell Biology 2020Quote: ... separated on a 3-8 % Tris-Acetate gel (Invitrogen) by SDS-PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... and Y14 siRNA (5’-GGGUAUACUCUAGUUGAAUUUCAUAUUCAACUAGAG-3’) with Lipo2000 (Invitrogen) in Optimem (Gibco) ...
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bioRxiv - Cancer Biology 2020Quote: ... and PC-3 cells were cultured in RPMI1640 (Gibco) with 10% FBS and 1% Antibiotic-Antimycotic ...
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bioRxiv - Microbiology 2020Quote: ... epidermidis (Thermo scientific; 3 μg/ ml in 1% BSA). MSC’s were labelled with anti-vimentin monoclonal antibody (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... counterstained with DAPI or TO-PRO-3 (Thermo Fisher), and coverslipped with Fluoromount-G mounting medium (Southern Biotech ...
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bioRxiv - Developmental Biology 2021Quote: ... anti–glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Thermo Fisher Scientific), anti-FLAG (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1.1ul (4U) FastDigest-HindIII (prediluted 1/3) (Thermo Fisher) and 11.1ul 20X primer-probe mix ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS with 3% bovine serum albumin (BSA) (ThermoFisher), 0.4% saponin ...
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bioRxiv - Molecular Biology 2020Quote: ... 3-8% Tris-Acetate gels (Thermo Fisher Scientific, EA0375PK2) were used ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 µL of Lipofectamine RNAiMAX Reagent (Invitrogen, 13778-150) was diluted in 50 µL of Opti-MEM (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... anti-desmoglein-3 mouse monoclonal (Invitrogen, Carlsbad, CA, USA), anti-desmoglein-1 rabbit polyclonal (Abclonal ...
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bioRxiv - Molecular Biology 2020Quote: ... dialysed (Snakeskin® dialysis tubing, 3 kDa MWCO, ThermoFisher) against water (1:10,000 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... in 3 ml of DMEM/F12 medium (Thermofisher Scientific). The pancreas tissue was further digested in DMEM/F12 supplemented with collagenase D (Sigma ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... and stained with TO-PRO-3 viability dye (ThermoFisher). Cells were then filtered through a 35 µm cell strainer (Corning ...
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bioRxiv - Biophysics 2021Quote: ... Mirius (1:3) in Opti-MEM media (Thermo scientific). Cell confluency was allowed to reach 70-80% prior to transfection ...
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bioRxiv - Neuroscience 2021Quote: ... and crystals of 3 kDa fluorescein dextran (D3306; Invitrogen) were applied to the proximal cut end of the chorda tympani ...
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bioRxiv - Cell Biology 2020Quote: ... anti-aggrecan antibody BC-3 was from Thermo Scientific and anti-decorin antibody ab175404 was from Sigma.
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bioRxiv - Microbiology 2021Quote: ... using QuantStudio 3 Real-Time PCR System (Applied Biosystems) according to instructions of the manufacturer ...
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bioRxiv - Bioengineering 2021Quote: ... using a QuantStudio 3 PCR system (Thermo Fisher Scientific). Osteoblast ...
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bioRxiv - Bioengineering 2021Quote: ... and 3 minutes in xylene (Fisher Scientific X5-500). 1-2 drops of CytoSeal mounting media (Thermofisher ...
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bioRxiv - Bioengineering 2021Quote: ... or rabbit anti-caspase-3 (1:500, ThermoFisher #700182) followed by Alexa-conjugated anti-mouse or anti-rabbit secondary antibodies for 8 hours each at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... 3) latrunculin-b (Lat-B; 2 μM; Fisher Scientific), 4 ...
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bioRxiv - Biochemistry 2020Quote: ... N-cyclohexyl-3- aminopropanesulfonic (CAPS) was from Acros Organics, 2-(N-Morpholino)ethanesulfonic acid (MES ...
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bioRxiv - Biophysics 2021Quote: ... counted using a Countess 3 FL cell counter (Invitrogen), centrifuged for 2 min at 200 g ...
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bioRxiv - Neuroscience 2021Quote: ... QuantaStudio 3 real-time PCR machine (Thermo Fisher Scientific) was used for qPCR analysis ...
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bioRxiv - Neuroscience 2021Quote: ... 3 µg of RNA using SuperScript II Kit (Invitrogen) were reverse transcribed to cDNA ...