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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for 3 Thiophen 3 yl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... viable CD45-CD34+Sca-I- cells were sorted from epidermal single cell suspensions of 1-month-old EGFRΔEgr2 (n=3) and littermate controls (n=3) into Trizol LS Reagent (Thermo Fisher Scientific) using a FACS Aria III ...
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bioRxiv - Microbiology 2023Quote: ... cells were blocked for 1 h in 3% BSA/PBS and incubated with rabbit-anti-GFP (1:50 in 3%BSA/PBS, 200 µl/well, Invitrogen, G10362) for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... HuH6 hCLDN1 [13] and Lunet N#3 and Lunet N#3 hCD81 [18] were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Thermo Fischer Scientific) supplemented with 1 % MEM non-essential amino acids (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... PCR products (1□μg) of desired templates were 3’ end-labeled using a Pierce biotin 3’ End DNA Labeling Kit (Thermo Scientific). The resulting probe reaction mixtures were electrophoresed on a 0.8% agarose gel for 30 min at 100 V and then gel purified with a NucleoSpin Gel and PCR Clean-up kit (Takara Bio USA) ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted and quantified from WwoxWT/WT and WwoxP47T/P47T n=6 mice/group (3 males and 3 females) and cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... King’s B (KB) medium was prepared by mixing 20 g/L of proteose peptone #3 (BactoTM Proteose Peptone No. 3, ThermoFisher Scientific), 1.5 g/L of dipotassium phosphate (Carl Roth GmbH + Co ...
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bioRxiv - Cell Biology 2024Quote: ... GAPDH-F (5’-GGA GCGAGATCCCTCCAAAAT-3’) and GAPDH-R (5’-GGCTGTTGTCATACTTCTCATGG-3’) using Power SYBR Green PCR Master Mix (Applied Biosystems). All reactions were carried out on the StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... Apoptosis (cleaved Caspase 3/7) measurements were done using the FMK-FLICA-Caspase 3/7 Kit (+ PI staining; Thermo Fisher Scientific) following the manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2 mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... and CYPBΔPSI (forward 5′ CGAACTTGTGTTTGGTGGTGTT-3′ and reverse 5′-GCCTTTTTCAGTCACCGGAACA-3′) primers on a StepOnePlusTM Real-Time PCR System (Applied Biosystems, USA) as previously described (Muthusamy et al ...
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bioRxiv - Molecular Biology 2024Quote: RNAs were extracted from whole testes of 3 Ptbp1fl/fl and 3 Ptbp1ΔSC mice at postnatal day (P) 35 using the PureLink RNA Mini Kit (Ambion, Cat. 12183025). RNA-seq libraries were constructed using Illumina’s TruSeq Stranded mRNA sample prep kit ...
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bioRxiv - Immunology 2024Quote: ... slides were washed with 1:3 diluted blocking buffer (3-times, 1 minute) and incubated with anti-mouse Alexa fluor-594 antibody (Invitrogen, A21203), anti-rabbit Alexa fluor-750 (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... liver and muscle tissues from a total of 72 fish (n = 8 salmonid species x 3 biological replicates x 3 tissues) using TRIzol (Invitrogen/Life Technologies) with glass mill beads (1mm ...
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bioRxiv - Cell Biology 2024Quote: ... human CENP-E ON-TARGETplus SMART pool siRNA (L-003252-00-0010, human custom-made 3’UTR HEC1 siRNA (oligo #3, Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... before blotting with a force of −3 for 3 sec under 100% humidity at 4C (Vitrobot Mark IV, Thermo Fisher Scientific). Grids were immediately plunge-frozen in liquid ethane and stored in liquid nitrogen until imaging ...
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bioRxiv - Cancer Biology 2021Quote: ... clone IMAGE ID 4977050 was obtained from Source Bioscience and PCR cloned using oligos (Tet2fwd: 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTTAatgccaaatggcagtacagt-3’ and Tet2rev: 5’-GGGGACCACTTTGTACAAGAAAGCTGGGTTtcatacaaatgtgttgtaag-3’) into pDonor221 (Invitrogen Gateway, ThermoFisher) and sequenced ...
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bioRxiv - Cell Biology 2020Quote: ... or a 1:1 mixture of two siRNA to CHMP2A (5’-CAGGCCGAGAUCAUGGACAUG-3’ and 5’-GAAGAUGAAGAGGAGAGUGAC-3’) using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: MiaPaCa-2 cells were seeded in 12-well plates and transfected with 3 µg of DNA (NUPR1-GFP, NUPR1-Flag or control vector) and 3 μL of Lipofectamine 3000 Transfection Reagent (Thermo Fisher Scientific) per well ...
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bioRxiv - Developmental Biology 2021Quote: ... and the reverse primer containing the Sp6 promoter (5’ ATTTAGGTGACACTATAGCCTCTTCAGTTCCTTCTTCCATC-3’).The aqp1a.1 probe was generated from whole extracted cDNA at 30hpf and amplified with the primers (5’-GTCATGAACGAGCTGAAGAGC-3’) and (5’-GGGTCACTTTGAGGACATCTC-3’),incorporated into the pCR-BluntII-TOPO vector (Invitrogen, 45-0245) (containing both the SP6 and T7 promoters) ...
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bioRxiv - Developmental Biology 2021Quote: ... were transfected with pPyCAG-(HA)3-Nanog-IRES-Puro and pPyCAG-(Flag)3-Resf1-IRES-Puro using Lipofectamine 3000 (Invitrogen cat. L3000001). In parallel ...
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bioRxiv - Genomics 2020Quote: ... The 16S rRNA gene was amplified from the extracted genomic DNA using 16S universal primers 27F (5’-AGA GTTTGATCMTGGCTCAG-3’) and 1492R (5’-GGTTACCTTGTTACGACTTC-3’) and sequenced using an automated ABI3730XL capillary DNA sequencer (Applied Biosystems, USA) for taxonomic identification at Cosmo Genetech (Seoul ...
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bioRxiv - Neuroscience 2021Quote: All females were ovariectomized and primed for 3 days with subcutaneous administration of estradiol benzoate (17-β-Estradiol-3-Benzoate, Fisher Scientific, 2 μg dissolved in sesame oil starting 3 days prior to experiments) ...
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bioRxiv - Cancer Biology 2020Quote: ... n = 3) or oligopyridylamides (5 µM ADH-1 or ADH-6, n = 3) using a combination of both TriZol (Thermo Fisher Scientific) and RNAeasy Mini Kit (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: Cross-linking experiments of Sgo11-415 and CPCISB10-280 were performed using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Fisher Scientific) in the presence of N-hydroxysulfosuccinimide (NHS ...
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bioRxiv - Microbiology 2020Quote: ... 229E-RP reverse primer (5’-CCAACACGGTTGTGACAGTGA-3’) and the TaqMan minor groove binder (MGB) probe 229E-TP (FAM-5’-TCCTGAGGTCAATGCA-3’-NFQ-MGB; Thermo Fisher Scientific), derived from the HCoV 229E membrane protein gene sequence as described previously (Vijgen et al. ...
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bioRxiv - Microbiology 2021Quote: The mcr-1 gene was amplified from the clinical isolate ESBL20150072 (European Nucleotide Archive, accession number; SAMEA104060671) using primers: 5’-TTTTGGATCCCGGTTTTCGGGCTTTTTA-3’ and 5’-ATATGTCGACTCAGCGGATGAATGCGGT-3’ and Phusion polymerase (Thermo Fisher Scientific). PCR product was digested with BamHI and SalI and ligated into pACYC184 digested with the same enzymes (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... PCSK9 mRNA expression was assessed by real-time PCR using PCSK9 primer sets 5’-TGTCTTTGCCCAGAGCATC-3’ and 5’-GTCACTCTGTATGCTGGTGTC-3 (Integrated DNA Technologies) and SYBR Select Master Mix (ThermoFisher, ABI 4472908).
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bioRxiv - Biochemistry 2022Quote: ... was mixed with 10 nm gold nanoparticles and was applied to the glow discharged surface and blotted at 4 °C and 100% relative humidity for 3 s and a blot force of −3 using the Vitrobot IV System (Thermo Fisher Scientific). Grids were plunged in 100% liquid ethane ...
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bioRxiv - Genetics 2022Quote: ... 5’-GGCAAGCUGACCCUGAAGUdTdT-3’ / 5’-ACUUCAGGGUCAGCUUGCCdTdT-3’ or with a hsa-miR-34a mimic duplex: 5’-P-UGGCAGUGUCUUAGCUGGUUGUU-3’ / 5’-P-CAAUCAGCAAGUAUACUGCCCUA-3’ according to the protocol for Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2021Quote: ... a fragment containing 2.21 kb upstream regulatory region of ELF3 was amplified from Col-0 genomic DNA using primers ELF3Prom-Fw (5’-CACCCTTATAAATAAAATTCC-3’) ELF3Prom-Rv (5’-CACTCACAATTCACAACCTTTTTC-3’) and cloned into the Gateway System (pENTR™ Directional TOPO® Cloning kit, Invitrogen) to obtain the pELF3-TOPO plasmid ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary neurospheres grown for 3 days (3-4 million cells) were harvested and crosslinked with 0.5% formaldehyde (Thermo Fisher, cat. no. 28908) in 0.1 M DPBS for 5 min at RT with soft agitation ...
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bioRxiv - Cell Biology 2022Quote: ... The C2C12 cells were transfected with 2 μg plasmids (L-Cas9n-EGFP:R-Cas9n-puro=3:1) by electroporation (1650 v/10 ms/3 pulses) with the Neon Transfection System Kit (Thermo Fisher Scientific). The cells were seeded overnight to 24-well plates containing an antibiotic-free complete mediums ...
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bioRxiv - Plant Biology 2022Quote: 5’-biotinylated RNA and unmodified RNA of Motif 3 (5’-AAAUCGCCGGAG-3’ 5’-Bio-AAAUCGCCGGAG-3’) were synthesized by Eurofins (Hamburg, Germany) and used for LightShift™ Chemiluminescent RNA EMSA Kit (Thermo Scientific). Total protein was extracted from LL and 10 min LL➔HL-treated plants using extraction buffer (25 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... the hypervariable V3 region of the 16S rDNA gene was amplified from 20 ng of DNA using the primers 5′-CCTACGGGAGGCAGCAG-3′ and 5′-ATTACCGCGGCTGCTGG-3′ (Integrated DNA Technologies BVBA, Leuven, Belgium),(18) 1U Platinum® PCR SuperMix High Fidelity (ThermoFisher Scientific) and 10 μM of primer-mix ...
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bioRxiv - Neuroscience 2021Quote: ... Drosophila dSF2 sequence was PCR amplified (dSF2 F 5’-CACCATGGGATCACGCAACGAGTGCCG-3’ and dSF2 R 5’- ATAGTTAGAACGTGAGCGAGACCTGG-3’) was cloned into pEntry-TOPO vector (Thermo Fisher Scientific). The pENTR-dSF2 vector was recombined with Gateway plasmid pTWH (Drosophila Genomics Resource Center ...
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bioRxiv - Genetics 2021Quote: ... SDS-Page separation was completed by running between 3 and 10μg of total protein on a NuPAGE 3%–8% Tris-acetate gel (Thermo Fisher Scientific Scientific). Next ...
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bioRxiv - Microbiology 2023Quote: ... and 40 cycles of PCR (95°C for 3 seconds, 60°C for 30 seconds) on a QuantStudio 3 (Applied Biosystems, MA).
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bioRxiv - Biochemistry 2023Quote: ... samples were blotted at 100% humidity for 3 s (13°C, 0 s drain time, blot force -3 to +2) with a Vitrobot Mark IV (Thermo Fisher Scientific) and plunged into liquid ethane ...
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bioRxiv - Cell Biology 2023Quote: Supernatants of cell culture medium collected during medium exchanges were analysed for albumin (10242, Diagnostic Systems) and 3-beta-hydroxybutyrate (Autokit 3-HB, Fujifilm Wako) on an Indiko Plus chemical analyzer (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... and N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (BODIPY FL DHPE) were purchased as solids from Invitrogen (Thermo Fischer Scientific), Avanti Polar Lipids ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 μl of sample was saved for 3′ -biotin labeling for visualization using Pierce RNA 3′ End Biotinylation Kit (Life Technologies 20160) according to manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: ... 2 µl cDNA was amplified with TaqMan™ qPCR (38 cycles, 3 technical replicates of 3 biological replicates) using master mix (Thermo Fisher) and the following TaqMan assays ...
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bioRxiv - Immunology 2024Quote: ... and SB28 RRV-IRF8 (100% transduced) cells were plated at 1x105 cells (n=3 per time point) and counted (Thermofisher Countess 3)at 24 and 48 hours post-seeding ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of plasmid and 3 μL of Lipofectamine 2000 (m/v = 1:3) were diluted in 0.1 mL of Opti-MEM (Gibco, 31985070, Gaithersburg, MD). After 5 min of incubation ...
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bioRxiv - Microbiology 2024Quote: ... was added at a 1:2000 dilutions for 1 h at 37 °C, followed by adding TMB (3, 3, 5, 5′-tetramethylbenzidine) peroxidase substrate (Thermo Fisher Scientific) for 30 min ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was lawned with goat anti-human IgG Fc (50 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was coupled directly to mAbs diluted to 10 µg/mL in 10 mM sodium acetate (pH 4.5 ...
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bioRxiv - Neuroscience 2023Quote: ... Neuron-cancer cocultures were quantified for neuronal outgrowth by identical image generation and processing with additional pre-incubation (3 drops/mL for 90 min at 37◦C) of NucRed Dead 647 ReadyProbes™ Reagent (TO-PRO-3 iodide) (Invitrogen, #R37113) for dead cells and NucBlue Live ReadyProbes™ Reagent (Hoechst 33342 ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...