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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for rno mir 125b 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Quantitative RT-PCR analysis was performed using Power SYBR Green PCR Master Mix (Applied Biosystems, Life Technologies) and an Applied Biosystems ABI 7500 Real-Time PCR system using the primers listed in the supplementary material ...
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bioRxiv - Developmental Biology 2024Quote: ... Quantitative RT-PCR analysis was performed using Power SYBR Green PCR Master Mix (Applied Biosystems, Life Technologies) and an Applied Biosystems ABI 7500 Real-Time PCR system using the primers listed in the supplementary material ...
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bioRxiv - Cell Biology 2024Quote: ... RT-PCR for gel electrophoresis was performed using Platinum II Hot-Start Green PCR Master Mix (ThermoFisher), and products separated on 2% EX e-gels containing SYBR Gold II (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... Quantitative RT-PCR (qRT-PCR) analyses were conducted on a StepOnePlus system (Applied Biosystems, Foster City, CA) employing SYBR Premix Ex Taq (Takara Bio) ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative RT-PCR was performed with TaqMan fast universal PCR master mix (Applied Biosystems; Foster City, CA). RT reaction conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR of RT products (qRT-PCR) was conducted using VeriQuest SYBR Green qPCR Master Mix (Affymetrix). Peptidylprolyl isomerase B (Ppib ...
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bioRxiv - Microbiology 2023Quote: ... Samples were processed using commercial software for rt-PCR (QuantStudio Real-Time PCR Software v1.3, Applied Biosystems). Ct values of egfp expression were compared among treatments using generalized linear modelling ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR and quantitative PCR (qPCR) were performed using an ABI7500 qPCR instrument (Life Technologies, Carlsbad, CA) and a PrimeScript RTreagent kit with gDNA Eraser and SYBR Premix Ex Taq II (TliRNase H Plus ...
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bioRxiv - Neuroscience 2020Quote: ... Transcripts abundance was determined by reverse transcription quantitative PCR (qRT-PCR) using the SYBR® Green RT-PCR assay (Applied Biosystems). Amplification ...
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bioRxiv - Microbiology 2020Quote: ... and quantitative real-time RT-PCR (qRT-PCR) was performed using the ViiA 7 real-time PCR detection platform (Applied BioSystems). Primers were based on previous studies (Ding et al. ...
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bioRxiv - Pathology 2019Quote: ... and quantitative real-time RT-PCR (qRT-PCR) was performed using the ViiA 7 real-time PCR detection platform (Applied BioSystems). All gene specific primers used for qRT-PCR were evaluated by BLASTN search against the Fg and Bd genomes to exclude unspecific annealing and are listed in Supplemental Table 2 ...
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bioRxiv - Microbiology 2021Quote: ... and 9.25 µL sterile water using the standard Power SYBR Green PCR Master Mix RT-PCR Protocol (Protocol Number 436721) on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher). As positive controls for the WSP and WPCP primer sets ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time polymerase chain reaction (RT-PCR) was performed using SYBR Green PCR Master mix (Applied Technologies) on QuantStudio3 Real-Time PCR System (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... Quantitative RT-PCR was performed using a standard TaqMan® PCR protocol on a StepOne real time PCR System (Applied Biosystems) with primers specific for murine Dag1 ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR (qRT-PCR) was performed on the Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) using the QuantiTect SYBR Green® RT-PCR kit (204243 ...
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bioRxiv - Immunology 2022Quote: ... followed by staining for 30 minutes with antibodies to the different TF at RT using the Foxp3/TF staining buffer set (ThermoFisher). Samples were acquired on the Aurora (Cytek).
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bioRxiv - Immunology 2020Quote: ... extravesicular cDNA was pre-amplified for all miRNA except the spike ath-miR-159 by 10-14 cycles of PCR using Taqman probe reagent (Solisbiodyne, Tartu, Estonia) and Taqman assays (Fisher Scientific), followed by 40 cycles of PCR on an ABI7300 instrument (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... and Real-time PCR of miR expression was carried out in a final volume of 10 µl using TaqMan MicroRNA Assays (Applied Biosystems) and normalized on RNU48 and RNU49 as endogenous controls ...
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bioRxiv - Molecular Biology 2022Quote: ... Reverse Transcription and quantitative PCR of mature miR-211 from total RNA was performed with TaqMan small RNA Assay (Applied Biosystems) according to the manufacturer’s instruction and carried out on Quant Studio 6 Flex System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: Fragments of the 3’UTR regions of predicted miR-324 targets were PCR amplified (using Phire Hot Start II DNA Polymerase; ThermoFisher Scientific) from murine genomic DNA or osteoblast cDNA and cloned by In-Fusion HD cloning (Takara Bio ...
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bioRxiv - Neuroscience 2020Quote: ... 1 μg of RNA was subjected to RT reaction using the SuperScript first-strand synthesis system for RT-PCR (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... One-step RT-qPCR was performed using One-Step Real-Time RT-PCR Master Mixes (Thermo Fisher Scientific, Waltham, USA) and the StepOnePlus Real Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... RT-qPCR analysis was performed using Power SYBR Green RNA to CT 1 step RT-PCR kit (Applied Biosystems, #4389986) on a QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Peptide quantification was done using Pierce™ Quantitative Fluorometric Peptide Assay and equal amount from each sample was labelled using TMTproTM 16plex Label Reagent Set Set (GP2d) or TMTproTM 10plex Label Reagent Set Set (Mia Paca-2) (Thermo Fisher Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Twenty thousand cells were plated and then transfected with pre-miR-100 or control miRs in combination with the SMRT/NCOR2 expression vector (pSMRT, Fisher Scientific) after 1 day of growth ...
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bioRxiv - Immunology 2021Quote: ... were co-transfected with the EGFP-Caspase 2 or EGFP-Δ Caspase 2 plasmid and the synthesized miR-2 (miR-2-mimic) using Cellfectin II transfection reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... and the synthesized miR-137 or miR-7847 (50 nM/well) were co-transfected into S2 cells using with Cellfectin II Reagent (Invitrogen, USA) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... myotubes were transfected with either 20nM of miR-30a or miR-30c mimics or 20 nM of a mimic scramble (mirVana™ miRNA mimics, ThermoFisher) using Lipofectamine® RNAiMAX Transfection Reagent (Ambion ...
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bioRxiv - Cancer Biology 2023Quote: ... the expression of miR-214 in HNSCC cells was silenced using a pre-designed anti-miR-214 inhibitor (Thermofisher, Waltham, MA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The IgG PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies) in a total volume of 50 µl ...
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bioRxiv - Immunology 2021Quote: ... The IgG PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies) in a total volume of 50 µl ...
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bioRxiv - Cell Biology 2021Quote: ... Reactions with appropriate oligonucleotides were set-up with SYBR Green PCR Master Mix (Applied Biosystems) and carried out on an ABI 7500 Fast Real-Time PCR System ...
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bioRxiv - Immunology 2022Quote: ... qPCR reactions were set up using Power SYBR Green PCR Master Mix (Applied Biosystems/ ThermoFisher). Ct values and melt curve analyses were performed in the OHSU Gene Profiling Shared Resource with a QuantStudio 12K Flex system (Applied Biosystems/ThermoFisher).
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bioRxiv - Immunology 2022Quote: ... qPCR reactions were set up using Power SYBR Green PCR Master Mix (Applied Biosystems/ ThermoFisher). Ct values and melt curve analyses were performed in the OHSU Gene Profiling Shared Resource with a QuantStudio 12K Flex system (Applied Biosystems/ThermoFisher).
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bioRxiv - Microbiology 2020Quote: ... The Ig PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Thermo Fisher) in a total volume of 50 µl ...
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bioRxiv - Microbiology 2019Quote: ... The immunoglobulin PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies) in a total volume of 50 µl ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Real time PCR reactions were set up using FastStart Universal SYBR Green Master Mix (Invitrogen) and thermal cycling performed on a StepOnePlus Real-Time PCR System using Step One software v2.0 ...
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bioRxiv - Neuroscience 2021Quote: ... The reaction was set in the 7900HT Fast Real Time PCR System (Applied Biosystems, USA). qRT-PCR was performed in triplicate ...
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bioRxiv - Molecular Biology 2024Quote: ... Each reaction well was set up using SYBR™ Green PCR Master Mix (Applied Biosystems), 50 nM of each forward and reverse primer and 1 µL of cDNA in a 15 μL reaction volume ...
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bioRxiv - Molecular Biology 2024Quote: ... Each reaction well was set up using SYBR™ Green PCR Master Mix (Applied Biosystems), 50 nM of each forward and reverse primer and 10 ng gDNA in a 15 μL reaction volume ...
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bioRxiv - Cell Biology 2023Quote: ... Reactions were set in technical triplicates in a StepOnePlus Real-Time PCR system (Applied Biosystems). Relative gene expression was calculated with the 2-ΔΔCt method ...
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bioRxiv - Developmental Biology 2019Quote: ... qRT-PCR analysis was performed using gene-specific primers and SYBR Green PCR Master Mix (Applied Biosystems, 4309155). The sequences of the primers used are available upon request ...
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bioRxiv - Immunology 2021Quote: ... PCR was carried out with gene-specific primers (Table 1) using Phusion Hi-Fidelity PCR Mastermix (Thermo Scientific).
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR analysis was performed using gene-specific primers and SYBR Green PCR Master Mix (Applied Biosystems, 4309155). The sequences of the primers used are available upon request ...
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bioRxiv - Plant Biology 2020Quote: ... Primers and probes were designed using the Primer Express 3.0 software for Real-Time PCR (Applied Biosystems Foster City, California, USA), selecting the option MGB TaqMan probes with amplicon length 60 to 80 bp ...
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bioRxiv - Plant Biology 2020Quote: ... Primers and probes were designed using the Primer Express 3.0.1 software for Real-Time PCR (Applied Biosystems, Foster City, California, USA), selecting the option TaqMan® Quantification probes ...
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bioRxiv - Cancer Biology 2022Quote: ... mixed with indicated cDNAs and primers (primer sequences are listed in Supplemental Table S1) in a QuantStudio Real-Time PCR system (Applied Biosystems). Expression levels were normalized by 18S rRNA.
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bioRxiv - Microbiology 2022Quote: ... The oligo pool was then used for an asymmetric PCR with limited barcoded primer pool and excess reverse primer using AcuuPrime® PfX polymerase (Invitrogen) to incorporate most of the barcoded oligos in the hygR amplicon ...
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bioRxiv - Pathology 2022Quote: ... mRNA expression was measured by real-time PCR using either SYBR Green primers (IDT, Coralville, IA) or TaqMan primers (Life Technologies). SYBR Green Primer sequences were obtained from Primer Bank (http://pga.mgh.harvard.edu/primerbank/citation.html) ...
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bioRxiv - Microbiology 2023Quote: ... and SARS-CoV-2 RdRP (F2 primer IDT catalogue 10006860 and R1 primer IDT catalogue 1000688) using the Power SYBR Green PCR Master Mix (Thermo Fisher), and analyzed by QuantStudio 5 Real-Time PCR (Thermo Fisher) ...