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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... eight 12-d-old seedlings were collected and ground in 1×NativePAGE Sample Buffer (Invitrogen) containing 0.5% n-dodecyl β-D-maltoside (DDM ...
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bioRxiv - Molecular Biology 2023Quote: ... after which fucosidase production was induced with 1mM isopropyl β-d-1-thiogalactopyranoside (R0309, Invitrogen). Afterward ...
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bioRxiv - Immunology 2024Quote: ... To enrich for MCL-1 binding protein immunoprecipitation was preformed using the Dynabeads Protein G IP Kit (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... were amplified from genomic DNA by PCR from strains carrying either a wild type copy of csr-1 or csr-1(ADH) and cloned in pENTR-D-TOPO (Invitrogen). Plasmids used for Multisite Gateway reaction are listed as follows ...
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bioRxiv - Microbiology 2021Quote: ... The freeze-dried material was dissolved in 300 μl of 6:1 (v/v) of methyl sulphoxide D6 (99.9% atom D + 1% tetramethylsilane, ACROS Organics):D2O (100% atom D ...
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bioRxiv - Genomics 2021Quote: ... The early migratory CNCCs were then transitioned to CNCC early maintenance media: 1:1 Neurobasal medium/D-MEM F-12 medium (Invitrogen), 0.5× B-27 supplement with Vitamin A (50× stock ...
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bioRxiv - Biophysics 2022Quote: ... Upon reaching an OD600 of 0.6 – 0.8 the cells were induced with 1 mM IPTG (isopropyl β-D-1-thiogalactopyranoside; Thermo Scientific) and expression was continued overnight (12–16 h ...
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bioRxiv - Physiology 2023Quote: ... and subsequently incubated with antibodies for intracellular markers or corresponding isotype controls (IL33-PE 1:200 IC3626P R&D, FOXP3-PE 1:200 12-5773-82 Thermofisher, Isotype-PE 1:200 12-4321-83 Thermofisher) overnight at 4°C ...
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bioRxiv - Physiology 2023Quote: ... and subsequently incubated with antibodies for intracellular markers or corresponding isotype controls (IL33-PE 1:200 IC3626P R&D, FOXP3-PE 1:200 12-5773-82 Thermofisher, Isotype-PE 1:200 12-4321-83 Thermofisher ...
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bioRxiv - Neuroscience 2024Quote: ... Cell suspensions were washed twice and resuspended in 1 mL of Dulbecco’s PBS (D-PBS) before viability staining using Live/Dead™ Fixable Aqua (1/1000; ThermoFisher) according to manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... The high cell density flask was inoculated using 1% volume from a saturated overnight culture and induced at mid-exponential phase (OD 0.5-0.8) with 1 mM Isopropyl ß-D-1-thiogalactopyranoside (IPTG, Fisher Scientific). The cultures were grown at 37 °C until induction followed by a 5 h growth at 30 °C and left at 18 °C overnight (P1 expression) ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were immunostained according to standard protocols [21] using a primary rabbit anti-human antibody directed against zona occludens-1 (ZO-1; 1:100, Thermo-Fisher Scientific, UK) and a AF488-conjugated secondary goat anti-rabbit antibody (1:500 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cas9 over expressing cells were transduced with lentiviruses expressing sgRNA targeting human hypoxanthine phosphoribosyltransferase 1 (HPRT1) (LentiArray™ CRISPR Positive Control Lentivirus, human HPRT, Thermo Fisher Scientific Cat # A32829). HPRT1 knockout cells shows resistance to 6-thioguanine (6TG ...
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bioRxiv - Cancer Biology 2021Quote: ... AlexaFluor 488 conjugated anti-human/mouse CD44 antibody (1:50 dilution, Molecular probes, A25528) was directly added into the cell culture medium of the cells to be stained ...
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bioRxiv - Genetics 2021Quote: ... donkey-anti-rabbit and mouse-anti-human IgG secondary antibodies (Molecular Probes, 1:1000), or NbALFA-Atto647 (NanoTag Biotechnologies ...
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bioRxiv - Developmental Biology 2021Quote: ... or of the rabbit anti-Human Rac1 polyclonal antibodies (1:40, Thermofisher PA1-091) were added in 1 % BSA-PBS and incubated O/N at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... and 5 μg.L−1 of epidermal growth factor (EGF) human recombinant (Thermo Fisher Scientific) (Wu et al ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 µg/mL ...
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bioRxiv - Systems Biology 2021Quote: ... followed by the secondary goat anti-human HRP-conjugated antibody (1:4000, A18805, Invitrogen). Stained proteins were detected using Immobilon Western Chemiluminescent HRP Substrate (Millipore ...
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bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were used: Mouse anti-human synuclein (1:5000, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... anti-CD9 (1:500, mouse monoclonal anti-human CD9, clone MM2/57, Invitrogen, AHS0902). The membranes were then washed three times with 0.1% Tween 20 in PBS for 5 minutes at RT ...
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bioRxiv - Cancer Biology 2022Quote: Human primary fibroblasts FIB-1 and FIB-2 were cultured in DMEM medium (Gibco) supplemented with 10% FBS and Pen-Strep antibiotics ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 633-conjugated goat anti-human antibody (1:500; A21091 from Life Technologies); Alexa Fluor 555-conjugated goat anti-rabbit (1:500 ...
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bioRxiv - Bioengineering 2020Quote: ... Viable human tumor tissue pieces of ∼1-2mm3 were thawed in prewarmed DMEM (Gibco) supplemented with 10% FCS ...
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bioRxiv - Cell Biology 2019Quote: The human colorectal cancer cell line DLD-1 was cultured in DMEM (Life Technologies) supplemented with heat-inactivated 10% FBS (Atlanta Biologicals) ...
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bioRxiv - Systems Biology 2020Quote: ... The human monocytic cell line THP-1 was maintained in RPMI 1640 medium (Gibco). All media were supplemented with 10% fetal bovine serum (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... and anti-human AlexaFluor 647 (1:500, 30 minutes, 4°C; Life Technologies, A21445), and resuspended in CellFix (1:10 in water ...
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-Human IgG Fc HRP Secondary Antibody (ThermoFisher Scientific Catalog #31423, 1:10,000), and Rabbit anti-Human IgG F(ab’)2 HRP Secondary Antibody (ThermoFisher Scientific Catalog #31482 ...
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bioRxiv - Biochemistry 2021Quote: ... and secondary anti-human IgG antibodies (1:2000) conjugated to Alexa fluor 488 (Invitrogen). Cells staining positive for Spike were measured by flow cytometry on a Becton Dickinson BD LSR II Special Order System Flow Cytometer With HTS Sampler ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa Fluor 647–conjugated goat anti-human (1:1000; A-21445; Thermo Fisher Scientific). Secondary antibodies were dilute in PBST and incubated for 30 minutes at RT ...
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bioRxiv - Microbiology 2022Quote: ... stained using FITC-conjugated F(ab’)2-Goat anti-human IgA (Invitrogen; 1/1000) in PBS + 0.1% BSA for 20 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa fluor 488 Goat anti-Human IgG Secondary (#A-11013, dilution 1:100, Invitrogen); APC anti-human CD4 Antibody (#357408 ...
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bioRxiv - Immunology 2022Quote: ... incubated with a 1:10,000 dilution goat anti-human IgG (H+L)-HRP (Invitrogen), and washed twice more before detection with ECL (ECL Plus Western Blotting Substrate ...
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bioRxiv - Microbiology 2023Quote: ... AlexaFluor 647-conjugated anti-human and AlexaFluor 594-conjugated anti-mouse (Invitrogen, 1:1,000) antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... and an Alexa Fluor 488- conjugated goat anti-human secondary antibody (1:200, Invitrogen) were then used to reveal the immunopositive structure ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa Fluor 647-conjugated recombinant human EGF (Invitrogen, #35351, Lot# 1700382, 1:1000) to label EGFR.
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bioRxiv - Biophysics 2023Quote: The gene encoding human A2AAR (1-316) was cloned into a pPIC9K vector (Invitrogen) at the BamHI and NotI restriction sites ...
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bioRxiv - Genomics 2023Quote: A serial dilution from 1 ng to 1,000 ng Human Liver Total RNA (ThermoFisher) was prepared to generate the various input samples ...
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bioRxiv - Immunology 2024Quote: ... and anti-human IgG Alexa Fluor™ 647 (ThermoFisher, Cat. #A-21445, 1:3000) for 10 min at room temperature in dark ...
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bioRxiv - Microbiology 2024Quote: ... Human THP-1 monocytes obtained from ATCC were grown in RPMI 1640 medium (Gibco) containing 2 mM L-glutamine ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant fibroblast growth factor (human FGF2) (10 ng/ml, Invitrogen) was added to Dulbecco’s Modified Eagle’s Medium (DMEM)/F-12 (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... Human T cells were activated with human CD3/CD28 Dynabeads (Invitrogen), at a bead:cell ratio of 2:1 and transduced using supernatant collected from Phoenix-AMPHO cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used for human samples were anti-human CD31 (#17031942, Invitrogen), anti-human CD45 (#17945942 ...
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant Epidermal Growth Factor 1-53 (EGF 1-53, 5 ng/ml, Thermo Fisher Scientific, catalog #17005-042) and 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2021Quote: ... T lymphocytes were purified using the Pan T cell isolation kit (Miltenyi Biotech) and activated with Dynabeads Human T-Activator CD3/CD28 (1:1 beads:cell) (ThermoFisher) in X-vivo 15 medium (Lonza ...
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bioRxiv - Immunology 2022Quote: Supernatants were collected 3 days post Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco) stimulation for analysis of cytokine production using a Luminex-based multiplexed assay (Th1/2/9/17 18-plex Human ProcartaPlex Panel ...
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bioRxiv - Genetics 2022Quote: ... Incubation with the primary antibody (SDC-1 (1:200, CD138 Mouse anti-Human, PE, Clone: MI15, Fisher Scientific, UK) for SynT and HLAG (1:100 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... slides were incubated for 1 hr with goat anti-human 555 (Thermo Fisher Scientific, A-21433; 1:1000 dilution) and goat anti-rabbit 488 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation of endogenous PRRC2B was performed using 1 μg (dilution factor 1:125) rabbit anti-human PRRC2B antibody (Invitrogen) followed by Magnetic dynabead protein A (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... and for human biopsies (see Fig. S1B and C) goat anti-ZO-1 (Thermo Fisher; PA5-19090; 1:200) and anti-laminin primary antibodies were used ...