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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for 6 Chloro 2 3 4 9 tetrahydro 1H pyrido 3 4 b indol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... mounted with 10 µL of ProLong Diamond with DAPI (4’,6-diamidino-2-phenylindole; Molecular Probes, Eugene, OR), and incubated for 24-48 hours prior to analysis ...
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bioRxiv - Zoology 2023Quote: ... The samples were stained by the 4’,6-diamidino-2-phenylindole (DAPI) and the rhodamine-conjugated phalloidin (ThermoFisher). The signals were detected by fluorescent microscopy (Nicon ...
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bioRxiv - Bioengineering 2023Quote: ... 6 mM L-glutamine (2 mM from Gibco 31600-091 and 4 mM from additional Gibco 25030-081), penicillin (100 U/μL) ...
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bioRxiv - Immunology 2023Quote: ... Slides were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and mounted with ProLong Diamond Antifade Mountant (ThermoFisher). Images were acquired using a 20X objective lens Zeiss LSM 880 Confocal Laser Scanning Microscope.
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bioRxiv - Biophysics 2023Quote: ... Sample were rinsed in PBS and incubated with 4’,6-diamidino-2-phenylindole (DAPI) (Thermo Scientific™, USA) for 5 mins and Alexa 594-phalloidin (Invitrogen™ ...
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bioRxiv - Synthetic Biology 2023Quote: ... 6 mM L-glutamine (2 mM from Gibco 31600-091 and 4 mM from additional Gibco 25030-081), penicillin (100 U/μL) ...
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bioRxiv - Cell Biology 2023Quote: ... HSPCs were incubated with 20µM NBD C6-Ceramide (6-((N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)amino)hexanoyl)Sphingosine) (Invitrogen) for 30 minutes at 4°C for Golgi staining or Cytopainter (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... For nuclear staining 0.5µg/µL of DAPI (4’, 6-diamidino-2-phenylindole, dihydro-chloride, Invitrogen. Cat. No.: D3571) was added along with secondary antibody ...
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bioRxiv - Synthetic Biology 2024Quote: ... 6 mM L-glutamine (2 mM from Gibco 31600-091 and 4 mM from additional Gibco 25030-081), penicillin (100 U/μL) ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were washed five times in PBST and counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, D1306) and mounted in Aqua-polymount (Polysciences Ince. ...
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bioRxiv - Microbiology 2024Quote: ... Then, 4′,6-diamidino-2-phenylindole (DAPI, Solabio) and Alexa Fluor 488 Conjugate Concanavalin-A (Invitrogen, CA, USA) were added to each slide and incubated for 5 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... containing 2 μM cell-permeant Fluo-4 acetoxymethyl ester (Fluo-4-AM, Thermo Fisher) with the addition of the equal quantity (1:1 v/v ...
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bioRxiv - Immunology 2019Quote: ... Hemocytes nuclei were then stained by incubating with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) (Thermo Fisher Scientific, Rockford, IL, USA) dissolved in PBS and washed thrice with PBS ...
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bioRxiv - Bioengineering 2023Quote: ... for 30 seconds at room temperature followed by 15 min staining with 1 µg/mL 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen, cat. no. D1306) before microscopic imaging ...
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bioRxiv - Neuroscience 2019Quote: ... They were then washed 3 times with PBS and incubated for 1h at room temperature in secondary antibody (1:1000; ThermoFisher, Cambridge, MA; R37117). They were then washed once with PBS ...
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bioRxiv - Biochemistry 2022Quote: ... and blotted for 4 to 6 seconds at 4°C and 100% humidity using the Vitrobot system (ThermoFisher), before plunging immediately into liquid ethane for vitrification ...
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bioRxiv - Neuroscience 2022Quote: ... at 200V for 1h for αBK and Bis-Tris 4-12% polyacrylamide gradient gels (Thermo Fisher NP0323) in MES-SDS running buffer (Thermo Fisher B0002 ...
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bioRxiv - Microbiology 2019Quote: ... pneumophila antisera (1:20,000) for 1h at 37°C in PBS containing 4% BSA followed by anti-rabbit IgG Alex Fluor 488 (Invitrogen, 1:500) for 1h at 37°C in PBS containing 4% BSA ...
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bioRxiv - Cell Biology 2023Quote: ... primary antibodies in 10% GS in 1xPBS overnight at 4°C followed by a 1h incubation with anti-mouse (A11003, Invitrogen, 1:800) secondary antibody in 10% GS in 1xPBS at room temperature ...
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bioRxiv - Pathology 2024Quote: ... Cells were subcultured at a 1:3 to 1:6 ratio using Gibco TrypLETM Express Enzyme (12605010, Fisher Scientific) for cell dissociation ...
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bioRxiv - Genetics 2022Quote: ... 2% B-27 and 1% N-2 supplements (Life Technologies) and supplements 20ng/ml BDNF ...
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bioRxiv - Microbiology 2022Quote: ... 2% B-27 and 1% of N-2 supplements (GIBCO), 1% L-glutamine ...
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bioRxiv - Neuroscience 2024Quote: ... 2% B-27 and 1% of N-2 supplements (GIBCO), 1% L-glutamine ...
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bioRxiv - Microbiology 2019Quote: ... JE-CVax hyper-immune and non-immune heat inactivated mouse serum samples (starting dilution of 1/9) were serially diluted 3-folds in RPMI 1640 medium (Gibco, ThermoFisher Scientific) supplemented with 4% low IgG serum ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were seeded at 3×103 cell/cm2 and maintained in LHC-9 media (ThermoFisher 12680013) at 37 °C with 85% humidity and 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... Lipin-1 siRNA 5’-GAAUGGAAUGCCAGCUGAA-3’ and 3’-UUCAGCUGGCAUUCCAUUC-5’ (Invitrogen; HSS118307 (Sigma); optineurin siRNA (Invitrogen 4392420) ...
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bioRxiv - Bioengineering 2023Quote: ... 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide (EDC; 22980) was purchased from Thermofisher Scientific (MA) ...
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bioRxiv - Biochemistry 2023Quote: ... NaHCO3 and 150 μL acetone containing 4 mg/mL 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide (L-FDAA, Thermo Scientific) was added ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted and quantified from WwoxWT/WT and WwoxP47T/P47T n=6 mice/group (3 males and 3 females) and cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: 4T1 and 67NR cells at 70-80% confluence were transfected with the shRNA constructs described above in FuGene 6 at a ratio of 3:2 per manufacturer’s instructions (Thermo Fisher). Pools of transfected cells were selected in media with 7 µg/ml puromycin ...
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bioRxiv - Plant Biology 2022Quote: ... from cauline leaves of flowering plants by measuring the quantity of 4-methylumbelliferone product generated from the substrate 4-methylumbelliferyl-b-D-glucuronide (Duchefa) on a fluorometer (Fluoroscan II; Thermo Scientific).
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bioRxiv - Microbiology 2020Quote: ... and resuspended in 0.1 ml of PBS supplemented with the lipophilic styryl membrane dye N-(3-triethylammoniumprpyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64, Molecular Probes, Invitrogen; 10 μg.ml-1) (Pogliano et al. ...
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bioRxiv - Microbiology 2021Quote: ... using QuantStudio 6 or 3 Flex Real-Time PCR System (Applied Biosystems). SARS-CoV-2 standards with known copy numbers were used to construct a standard curve and calculate copy numbers/mL or copy numbers/g.
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml IL-6 and 10 ng/ml IL-3 (Gibco). Inpp4b+/+ and Inpp4b-/- LSK were each retrovirally transduced with pMSCV-MLL-AF9-IRES-mVenus ...
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bioRxiv - Microbiology 2023Quote: ... using QuantStudio 6 or 3 Flex Real-Time PCR System (Applied Biosystems). SARS-CoV-2 standards with known copy numbers were used to construct a standard curve and calculate copy numbers/mL or copy numbers/g ...
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bioRxiv - Cell Biology 2020Quote: ... BN-PAGE was performed using NativePAGE 3-12% or 4-16% Bis-Tris gels and associated running buffer (Invitrogen). The cathode buffer was supplemented with 0.002% (w/v ...
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bioRxiv - Immunology 2021Quote: ... Excess liquid was blotted for 3 seconds with a force of -4 using a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
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bioRxiv - Neuroscience 2020Quote: ... third instar larvae were dissected and stimulated with a HL-3 solution of 90 mM K+/0.5 mM Ca2+/4 μM fixable FM1-43 (Invitrogen) for 1 min ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were loaded with culture medium containing 3 μM of the fluorescent calcium indicator Fluo-4/AM (Life Technologies) and Pluronic F-127 (0.2% w/v ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody incubation was performed for 3-4 days and DiD (Thermo Fisher Scientific-Molecular Probes L7781; 1µg/ml) labeling was added from the second day to show the whole brain morphology ...
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bioRxiv - Synthetic Biology 2021Quote: N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (NBD-PE) was supplied by Thermo Fisher Scientific Inc ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were subcultured every 3-4 days as follows: cells were washed with phosphate buffered saline (PBS, Life Technologies) and then incubated with trypsin (Life Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... RT reactions were performed with 3-4 µg total RNA using oligo-dT and SuperScript III RT (ThermoFisher Scientific). QPCR was performed as previously described [20] ...
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bioRxiv - Cell Biology 2019Quote: ... Liposomes containing PtdIns(4)P- or PtdIns(3)P were independently labeled with DiI and DiD (Thermo Fisher Scientific) lipid dyes and detected by excitation at 561 nm and 640 nm ...
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bioRxiv - Bioengineering 2019Quote: ... C12 (4,4-Difluoro-5-Methyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid;D3823, Thermo Fisher Scientific), was added to the culture medium for a duration of 60 min and pumped through the media systems at 80 µl/h via positive pressure provided by a syringe pump ...
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bioRxiv - Molecular Biology 2020Quote: ... heated at 95°C for 3 min and seprated on a Novex WedgeWell 4-12% Tris-Glycine Gel (Invitrogen). Proteins were transferred to an PVDF Transfer Membrane (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... After 3 days the overlay was removed and cell monolayers were fixed with 4% formaldehyde (28906, Thermo Fisher Scientific) at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Uptake of fatty acids was quantified after incubation with 1uM 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic acid (Bodipy-FL C16, Thermo Fisher) at 37 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Media was changed every 3 to 4 days and cells were passaged at nearly 90% confluence using 0.25% trypsin (Gibco). B16F0 cells were verified for mycoplasma contamination at Charles River Laboratories for cell line testing ...
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bioRxiv - Cell Biology 2021Quote: ... or 5 µM BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine) (Invitrogen, D3521) together with 5 µM delipidated BSA (as a delivery carrier ...