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Citations for Thermo Fisher :
9951 - 10000 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: Purified recombinant RSVF trimer was coated on Ninety-six well plates (Nunc MediSorp, Thermo Scientific) overnight at 4 C with 0.5 ug/mL in coating buffer (phosphate buffered saline (PBS ...
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bioRxiv - Bioengineering 2024Quote: Purified recombinant RSVF trimer was coated on Ninety-six well plates (Nunc MediSorp, Thermo Scientific) overnight at 4 C with 0.5 ug/mL in coating buffer (phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2024Quote: ... The freshly purified primary OPCs were plated onto Ø10-cm tissue culture plates (Fisher Scientific 08-772E ...
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bioRxiv - Molecular Biology 2024Quote: ... same amounts of SLO protein or SCPA were coated to MaxiSorp plates (Thermo Fisher Scientific) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ELISA was carried out using the 96-well MaxiSorp plates (Nunc Thermo Fisher Scientific Inc) coated with 1 µg/ml (volume 100 μl/well ...
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bioRxiv - Immunology 2024Quote: ELISA was carried out using the 96-well MaxiSorp plates (Nunc Thermo Fisher Scientific Inc) coated with 1 µg/ml (volume 100 μl/well ...
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bioRxiv - Microbiology 2024Quote: ... All coverslips and plates were labeled with A594-conjugated goat anti-mouse secondary antibody (Invitrogen). After permeabilization ...
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bioRxiv - Neuroscience 2024Quote: Co-culture experiments were performed in Corning Transwell (12-well) plates purchased from Thermo Fisher Scientific (Cat # 07-200-156) ...
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bioRxiv - Molecular Biology 2024Quote: The ELISA plate was read by a Varioskan LUX microplate reader (Thermo Fisher Scientific, Inc.). Values of each pair of duplicates were averaged ...
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bioRxiv - Biochemistry 2024Quote: ... and the experiments were performed at room temperature in a black 384-well plate (NUNC). Measurements were recorded on a CLARIOstar microplate reader (BMG LABTECH).
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bioRxiv - Systems Biology 2024Quote: ... and then eluted with Buffer B into a 96-well plate (Thermo Fisher Scientific, AB1300), and vacuum dried ...
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bioRxiv - Immunology 2024Quote: ... plates were washed and incubated with diluted horseradish peroxidase (HRP) conjugated anti-mouse IgM (Invitrogen), IgG (Millipore Sigma ...
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bioRxiv - Microbiology 2024Quote: ... and 100 µL per well were transferred to a white 96-well plate (Thermofisher 236105). This plate was brought out of the anaerobic chamber ...
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bioRxiv - Microbiology 2024Quote: ... plates were washed three times with PBST and then developed with TMB substrate (ThermoFisher Scientific). Reaction was stopped using 0.16 M sulfuric acid and absorbance was read at 450 nm wavelength ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cell lysates were transferred to a black/clear bottom 96-well plate (Thermo Fisher Scientific) and 100 μL of Steady-Glo luciferase assay system (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... MI was plated on 100mm plates containing potato dextrose agar (Fisher Scientific, Hampton, NH, USA). Plates were incubated in the dark at 25C for 7 days ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded in a 96-well plate and were cultured in OptiMEM (ThermoFisher #31985047) for 3 days ...
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bioRxiv - Neuroscience 2024Quote: ... Larvae were placed in a 48-well plate (one larva per well, Fisher Scientific, USA) filled with 500 μL of embryo medium ...
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bioRxiv - Biophysics 2021Quote: ... The enzyme-dye mixtures were purified according to protocol included with the Antibody Conjugate Purification Kit for 0.5-1 mg (Thermo Fisher Scientific). The buffer was replaced with 50 mM HEPES (pH 7 ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesised from 1 μg of RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Thermo Fisher Scientific) as per the manufacturers protocol ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesised from 1 μg of RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Thermo Fisher Scientific) as per the manufacturers protocol ...
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bioRxiv - Cell Biology 2020Quote: To separate the nuclear from the cytoplasmic fraction differentiated 3T3-L1 adipocytes were harvested and 1 million cells were used to isolated the fractions using the PARIS kit (Life technologies) according to the manufacturers instructions ...
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bioRxiv - Cell Biology 2020Quote: ... according to the manufacturer’s instructions and 1 μg was reverse transcribed using the High-Capacity cDNA Reverse Transcription kit (Thermo Fisher, USA). qRT-PCR was performed using 25 ng cDNA per reaction and the Power SYBR Green PCR Master Mix (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μg of total RNA was used to generate cDNA using the High-Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific). The qPCR reaction was performed on a qTOWER3 real-time thermal cycler (Analytik Jena ...
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bioRxiv - Immunology 2021Quote: ... the samples were rinsed three times and stained for PD-1/PD-L1 proteins using an Alexa Fluor 647 Tyramide SuperBoost kit (#B40926, ThermoFisher Scientific). Firstly ...
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bioRxiv - Genetics 2021Quote: ... was used to demonstrate differences in mitochondrial membrane potential (MitoProbeTM JC-1 Assay Kit for Flow Cytometry, Invitrogen cat. number M34152). In brief ...
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bioRxiv - Molecular Biology 2021Quote: ... Amplicons were purified with Agencourt AMPure XP beads in 0.7:1 ratio (beads to PCR reaction volume) and quantified with the Quant-iT™ High-Sensitivity dsDNA Assay Kit (Invitrogen). The amplicons were pooled together and sequenced on the Illumina HiSeq 2500 using HiSeq Rapid SBS Kit v2 (500 cycles ...
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bioRxiv - Developmental Biology 2021Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA with the Maxima First-Strand cDNA synthesis kit (Thermo Scientific) according to the manufacturer’s protocol ...
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Coloring inside the lines: genomic architecture and evolution of a widespread color pattern in frogsbioRxiv - Evolutionary Biology 2021Quote: ... The experiment was run using a StepOnePlus real-time PCR system and a Power SYBR Green RNA-to-CT 1-step kit (Applied Biosystems) on a volume of 20µl ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μg purified DNaseI-treated RNA was used for reverse transcription (RT) by High Capacity RNA-to-cDNA kit (Applied Biosystems). 1 μg purified DNaseI-treated RNA was used for reverse transcription (RT ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was eluted with nuclease free water at 12000 x g for 2 min after which 1 µg of RNA was transcribed into cDNA using the RevertAid H Minus First Strand Kit (ThermoFisher Scientific) and random primers ...
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bioRxiv - Immunology 2022Quote: ... used for adoptive transfer in NSG mice were further incubated for 30 min with 1 μM CellTracer CFSE cell proliferation kit (Invitrogen, #C34554) before extensive washings with DPBS and intravenous (IV ...
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bioRxiv - Immunology 2022Quote: ... and then subjected to measurement of IL-1β protein using the Mouse IL-1 beta-uncoated ELISA kit (Invitrogen, Waltham, MA), following manufacturer’s instructions.
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BET protein inhibition regulates macrophage chromatin accessibility and microbiota-dependent colitisbioRxiv - Immunology 2021Quote: ... Cells were washed with cold 1x PBS followed by staining with LIVE/DEAD Fixable Blue Dead Cell Stain Kit (1:1000; Invitrogen L23105). Cell cycle changes in (+)-JQ1 treated Il10-/- BMDMs were assessed using the BD Pharmingen BrdU Flow kit according to the manufacturer (559619) ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were mixed in a ratio of 3:1 and total RNA was extracted with Ribopure Yeast Kit (Ambion, Life Technologies) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2019Quote: ... Human IgG and IgM was quantified in blood serum (1:1000 dilution) and culture supernatants (DILUTION) using the Human IgG/IgM total ELISA Ready-SET-Go! Kits (Invitrogen/eBioscience). All ELISAs were performed as per manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2019Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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D-alanylation of Teichoic Acids in Bacilli impedes the immune sensing of peptidoglycan in DrosophilabioRxiv - Immunology 2019Quote: ... The reverse transcription was performed on 1 μg of RNA by using the RevertAid RT Reverse Transcription Kit (Thermo Fisher Scientific). cDNA was used as template in PCR reaction with three 10 μl technical replicates of each biological sample ...
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bioRxiv - Plant Biology 2019Quote: ... Sobic.002G302700 was sequenced via Sanger sequencing in the genotypes in Table 1 according to the BigDye Terminator Kit (Applied Biosystems). Primers for template amplification and sequencing are provided in S1 Table.
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bioRxiv - Microbiology 2019Quote: ... The PCR product was gel purified from a 1% agarose gel using a GeneJET gel extraction kit (Thermo Scientific, Dublin, Ireland) and ligated into pGEM-T Easy vector (Promega ...
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bioRxiv - Molecular Biology 2019Quote: mRNA was purified from 200 µg total RNA extracted from uninfected and infected Huh7 cells (MOI 1, 48 hours post-infection) using one round of polyA selection (Dynabeads mRNA purification kit; Thermo Fisher) and one round of rRNA depletion (NEBNext rRNA depletion kit ...
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bioRxiv - Microbiology 2020Quote: ... then permebalized with 0.01% Triton X-100 in 1X PBS for 20 min before staining with SiR-actin Kit (Spirochrome AG, Switzerland) for 1 hour and DAPI (Applied Biosystems) for 15 min ...
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bioRxiv - Biochemistry 2019Quote: Protein concentration in g ⋅ L−1 of human serum was calculated using PierceTM Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... then 1 μg of each was used as template for synthesis of dsRNA using a MEGAscript™ T7 Transcription Kit (ThermoFisher). The MEGAclear™ Transcription Clean-up Kit (ThermoFisher ...
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bioRxiv - Genomics 2021Quote: ... RNA (1 µg, quantified with NanoDrop) was reverse transcribed to cDNA with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... reverse transcription and 14 cycles of PCR amplification using Ion Xpress™ RNA-Seq Barcode 1-16 Kit (#4475485, ThermoFisher Scientific). Quantification of the library was performed using Qubit Fluorometer high-sensitivity kit (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... was used to generate amplified and biotinylated sense-strand cDNA from the entire expressed genome according to the GeneChip WT PLUS Reagent Kit User Manual (P/N 703174 Rev 1; Affymetrix Inc.). GeneChip HTA Arrays were hybridized for 16 h in a 45 °C oven ...
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bioRxiv - Systems Biology 2021Quote: ... Peptides from each individual case and the GIS pooled standard or bridging sample (at least 1 per batch) were labeled using the TMT 10-plex kit (ThermoFisher 90406) for ROSMAP BA9 tissues ...
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bioRxiv - Microbiology 2021Quote: ... treatment and 1 μg DNA-free RNA was reverse transcribed into cDNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) on a Mastercycler Thermocycler (Eppendorf ...
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bioRxiv - Cancer Biology 2021Quote: ... quantitative real-time PCR was performed on 100 ng input RNA using Power SYBR green RNA-to-Ct 1 step kit (Applied Biosystems) and primers specific for PSA (F ...