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Citations for Thermo Fisher :
9551 - 9600 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... Complementary DNA (cDNA) was synthesized from 1 µg of total RNA using High-Capacity cDNA Reverse Transcription Kit (ThermoFisher, 4368813). Quantitative real-time PCR was performed using Itaq Universal SYBR green master mix (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... cDNA was generated from 1 μg of total RNA using a High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). qPCR was performed using a ViiA7 system (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... Horseradish-peroxidase conjugated secondary antibodies diluted 1:5000 in 5% non-fat milk were used and visualized with SuperSignal West Pico or Femto substrate kits (ThermoFisher). The following commercial primary antibodies supplemented with 0.02% sodium azide were used for immunoblot analysis ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg of total RNA was reverse transcribed using High-Capacity cDNA reverse transcription kit (Thermo Fisher scientific, Waltham, MA) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... cDNA was synthesized from 1-2 μg total RNA with the Multiscribe High-Capacity cDNA Reverse Transcription Kit (ThermoFisher 4368814).
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bioRxiv - Immunology 2023Quote: ... and 14 cycles of PCR amplification using the Ion Xpress™ RNA-Seq Barcode 1-16 Kit (Thermo Fisher Scientific). Yield distribution of the libraries was measured with the Qubit 1x dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... 1 mL) and loaded onto a pre-equilibrated SCX column ICAT™ cartridge kit (Applied Biosystems-AB Sciex, USA, #4326752). The peptides were eluted using different concentrations (30 ...
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bioRxiv - Genomics 2022Quote: ... The quality of extracted DNA was visualized using 1% agarose gel electrophoresis and quantified using Quant-iT PicoGreen ds-DNA Assay kits (Thermofisher) and a fluorescent plate reader ...
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bioRxiv - Developmental Biology 2022Quote: ... Purified RNA (1 μg) was used for reverse transcription using the High-Capacity cDNA Reverse Transcription kit (4368814, Applied Biosystems). Complementary DNA (cDNA ...
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bioRxiv - Developmental Biology 2023Quote: ... The mRNA from plasmid #1 and #2 was transcribed using the mMESSAGE mMACHINE SP6 Transcription Kit (Thermo Fisher Scientific, AM1340) and purified with the NucleoSpin RNA Clean-up XS kit (Macherey-Nagel ...
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bioRxiv - Developmental Biology 2023Quote: ... Explants were incubated in the dark for 1 h in a click-kit reaction cocktail containing Azide Dye (Molecular Probes). This was followed by 3 washes in 3% BSA/PBS before imaging.
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bioRxiv - Developmental Biology 2023Quote: Quantitative real-time PCR was performed using the TaqMan® RNA-to-Ct™ 1-Step Kit (Thermo Fisher, #4392653) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... and SXL (1:500, a gift from Fatima Gebauer) antibodies using the Western Breeze kit following the manufacturer’s protocol (ThermoFisher Scientific). We quantified the relative expression of protein for SXL using the gel analysis tool in ImageJ software following the website’s guidelines 90 ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from 1 μg of DNAse-treated RNA using the Vilo cDNA synthesis kit (Invitrogen, Thermo Fisher Scientific). qPCR reactions were prepared with IDOL TaqMan assay (ID Hs00982312_m1 ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from 1 μg of DNAse-treated RNA using the Vilo cDNA synthesis kit (Invitrogen, Thermo Fisher Scientific). qPCR reactions were prepared with IDOL TaqMan assay (ID Hs00982312_m1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... incubated after for 2’ at RT with Stop Reagent (1:11, in 1XPBS, Alexa Fluor 488 Tyramide SuperBoost Kit, goat anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... washed again two times in 1XPBS 5’ each and incubated with Alexa Fluor Tyramide 594 (1:100, Alexa Fluor 594 Tyramide SuperBoost Kit, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently stained with surface antibodies and then either fixed using 1% paraformaldehyde or permeabilized using a FoxP3/Transcription factor fixation/permeabilization kit (ThermoFisher). Samples undergoing permeabilization were then stained with intracellular antibodies ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained for 10 min in 0.5 µL/mL calcein-AM and 2 µL/mL ethidium homodimer-1 from the Live/Dead assay kit (L3224, Invitrogen). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of RNA was reverse transcribed with the High-Capacity cDNA Reverse Transcription Kit (Thermo Scientific, Waltham, MA, USA). The amplified cDNA was diluted to 10 ng/μl ...
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bioRxiv - Biochemistry 2023Quote: ... A total 1 μg of extracted RNA was reverse transcribed using SuperScript IV VILO Master Mix kit (Thermo Fisher, USA), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Cells were incubated with 10 μM EdU for 1 h using a Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (cat. no. C10419; Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Exosomes were isolated from 1 ml of serum using a Total Exosome Isolation Kit (Invitogen, Thermo Fisher Scientific Inc., USA), according to the manufacturers protocol ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids encoding heavy and light chains were transfected into Expi293F cells in a 2:1 mass ratio using an ExpiFectamine 293 transfection kit (Gibco). Six days post-transfection ...
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bioRxiv - Plant Biology 2023Quote: RNA in situ hybridization was conducted using Invitrogen ViewRNA ISH Tissue 1-Plex and 2-Plex Assay kits (ThermoFisher Scientific) using the manufacture’s protocol optimized for Medicago root and nodules sections (Kulikova et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified RNA (1 μg) was converted to cDNA by random hexamers using Verso c-DNA Kit (Thermo Fisher Scientific) following manufacturers’ protocol ...
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bioRxiv - Microbiology 2023Quote: HTLV-1-infected T cell clones were stained with LIVE/DEAD Fixable Near-IR Dead Cell Stain kit (Invitrogen, L34976) to enabling gating on live cells and then crosslinked in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2023Quote: Mitochondrial depolarization was assessed on cells exposed to Venetoclax for 24h using the MitoProbeTM JC-1 assay kit (ThermoFisher, M34152) following manufacturer recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... Fly brains and iPSC cells were incubated in 1-μM MitoSox red dye (kit #M7514, Thermo Fisher Scientific, Waltham, MA) for 15 mins ...
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bioRxiv - Neuroscience 2023Quote: Fly brains were dissected and incubated in 1-μM MitroTracker red dye (kit #M-7512, Thermo Fisher Scientific, Waltham, MA) for 15 mins ...
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bioRxiv - Immunology 2023Quote: ... RNA (1 μg) was reverse transcribed to make complementary DNA using SuperScript™ VILO™ cDNA Synthesis Kit (Invitrogen, 11754) using random primers ...
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bioRxiv - Microbiology 2023Quote: ... Commercially available kits were used for assessment of the levels of Myeloperoxidase (Cat #EMMPO) and PAI-1 (Cat# EMSERPINE1) purchased from Invitrogen, TPA (Cat# ab233615 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μg of RNA was used to generate cDNA with a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, 4368813) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: Multiplex immunoassay was performed using a Luminex bead-based multiplex ELISA kit (ProcartaPlex Mouse Cytokine & Chemokine Panel 1 26plex, reference # EPXR260-26088-901, Invitrogen). Each sample was normalized to the total protein concentration determined by Bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2024Quote: qPCR was performed on cDNA generated with 1 µg of RNA with Maxima First Strand cDNA Synthesis Kit (ThermoFisher #K1672) according to the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2024Quote: ... 1 μg of RNA was reverse transcribed into cDNA using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, 4368814). Primers used to amplify the FH2 domains of DAAM1 are provided in Table S1 ...
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bioRxiv - Neuroscience 2024Quote: ... About 1 μg of RNA was used for cDNA preparation using the High-Capacity cDNAReverse Transcription Kit (4374966, Applied Biosystems). qRT-PCR was performed using Fast SYBR Green Master Mix (4309155 ...
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bioRxiv - Plant Biology 2024Quote: cDNA was synthesized from 1 µg of total RNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) following the manufacturer’s instructions and samples were stored at −20°C until used ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR reaction mixtures were set up with reagents from the Power SYBR Green RNA-to-Ct 1-step kit (ThermoFisher) with 25-50ng RNA per reaction and primers are listed in Table S2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Isolated RNA (1 mg, quantified with NanoDrop) was reverse transcribed applying the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher) following the manufacturer’s protocol ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... ExpiCHO cells were transiently transfected with the vector containing the gene encoding soluble spike glycoprotein ectodomain or CR3022 (using a heavy chain:light chain ratio of 1:2) using the Expifectamine 293 Transfection Kit (ThermoFisher #A29129), following the user manual ...
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bioRxiv - Microbiology 2024Quote: ... DNA for genotyping was extracted from whole blood (>1% parasitemia) typically using the Qiagen DNAeasy or GeneJet Genomic DNA purification kit (Thermofisher).
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bioRxiv - Microbiology 2024Quote: ... RT-Q-PCR was performed with the Power SYBR™ Green RNA-to-CT™ 1-Step Kit (Thermofisher Scientific). Primers were designed to target different genes of interests (e.g ...
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bioRxiv - Neuroscience 2024Quote: ... and EGFP with 2:2:1 (total 0.5 µg / coverslip) using the Lipofectamine 3000 transfection reagent kit (Thermo Fisher Scientific). Transfected cells were identified by EGFP signals ...
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bioRxiv - Genomics 2024Quote: ... The whole transcriptome library was amplified and barcoded using the IonXpressTM RNA-Seq Barcode 1-16 Kit (Thermo Fisher Scientific). The quantity of the amplified whole transcriptome library was assessed using the Ion Library TaqManTM Quantitation Kit (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2024Quote: ... Reverse transcriptions were performed from 1 µg RNA according to the manufacturer’s protocol (High-capacity RNA to cDNA kit, Applied Biosystems), using random priming ...
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bioRxiv - Neuroscience 2024Quote: ... and the success of the gene editing method was assessed using the T7 Endonuclease 1 (T7E1) assay kit (Invitrogen, Germany). Positive individuals from the resulting F1 generation were then crossed with wild-type fish to produce the F2 generation which were screened again with T7E1 assay ...
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bioRxiv - Cancer Biology 2021Quote: ... Real-time PCR was performed on an ABI Prism 7500 sequence detection system using a SYBR® Green PCR Master Mix (Applied Biosystems; Foster City, CA) and following the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were developed using Amersham ECL Prime Western Blotting Detection Reagent (RPN2232) or SuperSignal ELISA Femto Maximum Sensitivity Substrate (Thermo Scientific, Ref: 37075, Lot: UO282555) and the resulting chemiluminescence imaged with an Alpha Innotech MultiImage Light Cabinet (Alpha Innotech ...
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bioRxiv - Physiology 2022Quote: ... qRT-PCR reaction was performed using SYBR® Premix Ex Taq™ II (Tli RNaseH Plus) and was conducted on ABI 7500 Real-time Detection System (Thermo Fisher Scientific, USA). The PCR reaction was carried out with the following reaction conditions ...