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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 1mg of protein was used to pull down active RHO (RHO-GTP) using the Active Rho Pull-Down and Detection kit from Thermofisher by following the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... and processed for Click-iT EdU detection according to manufacturer’s protocol (Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit; Thermo Fisher). All samples were analyzed and sorted using an Aria Fusion (Becton Dickinson ...
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bioRxiv - Microbiology 2023Quote: ... PCR-based detection of the 16S rRNA (positive control) and rhlC genes was completed using the Phusion High-Fidelity PCR kit (ThermoFisher) following the manufacturer’s instructions with published primer sequences [42 ...
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bioRxiv - Neuroscience 2024Quote: ... the EdU detection was performed using the Alexa Fluor-647 Click-iT EdU Cell Proliferation Assay kit (Invitrogen Cat# C10424), following the manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: ... The membranes were then processed using stringency washes and developed using the Chemiluminescent Nucleic Acid Detection Module Kit (ThermoFisher Scientific) according to the manufacturer’s recommendations.
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bioRxiv - Microbiology 2020Quote: ... Amplification and detection were performed in an ABI 7500 Fast Sequence Detection System (Applied Biosystems, Foster City, CA, USA) under the following conditions ...
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bioRxiv - Microbiology 2022Quote: ... Amplification and detection of specific products were performed with the ABI Prism 7700 sequence-detection system (PE Applied Biosystems) with the following cycle profile ...
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bioRxiv - Immunology 2020Quote: ... Detection of active caspase 3/7 was accomplished using CellEvent™ Caspase-3/7 Red Detection Reagent (ThermoFisher Scientific). Sorted CD4SP Rag1GFP+ thymocytes were stained with CD5-PerCP-Cy5.5 (53-7.3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Real-time PCR with SYBR Green detection was performed using an ABI PRISM 7500 sequence detection system (Applied Biosystems). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were plunge frozen in liquid nitrogen cooled liquid ethane using a FEI Vitrobot Mark IV (Thermo Fisher Scientific) at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... the grids were flash-frozen in liquid ethane cooled by liquid nitrogen with Vitrobot (Mark IV, Thermo Fisher Scientific). For the NRPE-bound ABCA4 sample ...
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bioRxiv - Biochemistry 2022Quote: ... The nitrogen content of the dried samples was measured using a Flash 2000 Organic elemental analyzer (Thermo Scientific, USA). About 2-3 mg of the dried and milled material from each specimen (n=144 ...
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bioRxiv - Biochemistry 2022Quote: ... The grids were plunged and frozen into liquid ethane cooled by liquid nitrogen using Vitrobot Mark IV (Thermo Fisher) after blotted 6 second at condition of 8°C 100% humidity ...
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bioRxiv - Biochemistry 2021Quote: ... Both samples were plunge frozen in liquid ethane cooled by liquid nitrogen using a Vitrobot plunger (Thermo Fisher Scientific). The plunger was set to 3 sec blotting time and 100% humidity.
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bioRxiv - Cell Biology 2021Quote: ... The grids were blotted for 2.5 s or 3.0 s and flash-frozen in liquid ethane cooled by liquid nitrogen with Vitrobot (Mark IV, ThermoFisher). The prepared grids were transferred to a Titan Krios operating at 300 kV equipped with Gatan K3 detector and GIF Quantum energy filter ...
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bioRxiv - Immunology 2020Quote: ... The muscle tissue was snap frozen in liquid nitrogen and homogenized in 2mL TRIzol reagent (Thermo Fisher Scientific #15596026), then 1 mL of the homogenized muscle in TRIzol was processed following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... Sample were plunge frozen in liquid nitrogen cooled liquid ethane using a FEI Vitrobot Mark IV (Thermo Fisher Scientific) at 8°C ...
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bioRxiv - Biochemistry 2022Quote: ... and plunge-frozen in liquid ethane (pre-cooled with liquid nitrogen) using a Vitrobot Mark IV (FEI, Thermo Fisher Scientific - blotting time of 3 s ...
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bioRxiv - Cell Biology 2022Quote: ... Grids were transferred to liquid nitrogen for autogrid clipping and were clipped as per the manufacturer’s instructions (Thermo Fisher). Blue light stimulation prior to plunge freezing was performed on an Olympus CKX53 optical microscope fitted with a CoolLED pE-300white LED fluorescence module ...
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bioRxiv - Biophysics 2023Quote: ... Samples were plunge frozen in liquid nitrogen-cooled liquid ethane using an FEI Vitrobot Mark IV (Thermo Fisher Scientific) at 8°C ...
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bioRxiv - Bioengineering 2023Quote: ... which was recovered and dried under nitrogen gas at room temperature before adding acetone (99.8%, Thermo Fisher Scientific, UK) at a 1:1 ratio of the cultivation volume and incubating the resulting mixture at 30 °C and 350 rpm for four hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Flash frozen tissues were crushed in liquid nitrogen with plastic pestels (Fisher, Thermo Fisher Scientific, Inc., Waltham, MA, USA) prior to isolation ...
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bioRxiv - Microbiology 2024Quote: Cryo-electron microscopy data was collected at liquid nitrogen temperature using a Titan Krios electron microscope (Thermo Fisher Scientific), equipped with a K3 Summit direct electron detector (Gatan ...
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bioRxiv - Cancer Biology 2023Quote: Frozen tissues/tumor samples were ground in liquid nitrogen and total RNA was extracted using TRIzol™ Reagent (Invitrogen) according to the manufacturer’s protocol and purified using the RNAeasy® Plus Mini kit (Qiagen) ...
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bioRxiv - Genomics 2023Quote: ... The biopsy cores were either immediately frozen in liquid nitrogen or put into 1 ml of RNAlater (AM7020, Invitrogen) or CellCover (800-050 ...
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bioRxiv - Biochemistry 2020Quote: ... The purity of the EMX1 sgRNA was confirmed by electrophoresis on a 12% TBE-UREA-PAGE gel (Thermo Scientific). Concentrations were determined by A260nm using a NanoDrop instrument (Thermo Scientific).
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 37°C for 4 hours and run on a 6% TBE-Urea gel (Invitrogen, EC6265BOX). Bands of the appropriate size were excised ...
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bioRxiv - Biophysics 2021Quote: ... 5 mM DTT) and then mixed with 50 mM (final 6 mM) disuccinimidyl dibutyric urea (DSBU) (Thermo Fisher Scientific). Crosslinking reaction was incubated on ice for 2 hours and then was quenched by adding 1M (final 50 mM ...
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bioRxiv - Biochemistry 2022Quote: ... The urea-denatured LipA was incubated in 25-fold molar excess of fluorescein-5-maleimide (FM, Thermo Fisher Scientific) for 3 h at the ambient temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... The size and integrity of the biotinylated RNA were checked on a 6% Urea gel (Thermo Scientific, Darmstadt, Germany). Immediately before use ...
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bioRxiv - Molecular Biology 2020Quote: ... Equal amounts of total RNA were mixed with sample buffer (Novex 2x TBE-urea sample buffer, Invitrogen, Cat# LC6876) and loaded onto Novex TBE-Urea Gels 10% (Cat# EC68755 ...
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bioRxiv - Biochemistry 2021Quote: ... and for nucleic acids using a 12.5 % polyacrylamide/8 M urea gel visualized by SYBR Gold staining (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... Brain tissues were lysed in 8 M urea lysis buffer (8 M urea, 10mM Tris, 100 mM NaH2PO4, pH 8.5) with HALT protease and phosphatase inhibitor cocktail (ThermoFisher). Homogenization was performed using a Bullet Blender (Next Advance ...
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bioRxiv - Neuroscience 2023Quote: ... and six volumes of the tissue weight in urea lysis buffer (8LM urea, 10LmM Tris, 100LmM NaH2PO4, pH 8.5) containing 1X HALT protease inhibitor cocktail without EDTA (78425, ThermoFisher). Tissues were homogenized in a Bullet Blender (Next Advance ...
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bioRxiv - Cell Biology 2023Quote: ... Briefly, samples were first denatured for 2hrs at 37 °C in urea (8M, Fisher) and dithiothreitol (10mM, Thermo Fisher), continuously agitated ...
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bioRxiv - Immunology 2023Quote: ... Remaining protein pellets were dissolved with 8M urea and total protein was quantified using Pierce BCA assay (Thermo Fisher). Total protein amount was used for equivalent loading for High Performance Liquid Chromatography and High-Resolution Mass Spectrometry and Tandem Mass Spectrometry (HPLC-MS/MS ...
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bioRxiv - Immunology 2023Quote: ... Remaining protein pellets were dissolved with 8M urea and total protein was quantified using Pierce BCA assay (Thermo Fisher). Total protein amount was used for equivalent loading for High Performance Liquid Chromatography and High-Resolution Mass Spectrometry and Tandem Mass Spectrometry (HPLC-MS/MS ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... The resulting small RNAs were mixed with an equal volume of 2X NovexTM TBE-Urea sample buffer (ThermoFisher, #LLC6876), separated on a 17.5% polyacrylamide-urea gel ...
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bioRxiv - Microbiology 2019Quote: ... Amplicons were cleaned up and normalized with the SequalPrep Normalization Plate Kit (ThermoFisher Scientific) and pooled amplicons were quantified with the KAPA library quantification kit (KAPA Biosystems).
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bioRxiv - Physiology 2019Quote: ... Normalization was performed using a SequalPrep Normalization Plate Kit (Thermo Fisher Scientific, Darmstadt, Germany) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed and the substrate solution (TMB substrate Kit, Thermo Fisher Scientific # 34021) was added ...
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bioRxiv - Cell Biology 2021Quote: ... Nuclei were isolated from strain plates using a NEPER nuclear isolation kit (Thermo Scientific) according to manufacturer protocols ...
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bioRxiv - Microbiology 2020Quote: ... purified and normalized using a SequalPrep Normalization Plate (96) Kit (ThermoFisher Scientific, Waltham, MA). Samples ...
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bioRxiv - Evolutionary Biology 2022Quote: ... plates were scraped into PBS and plasmid isolated (GeneJET Plasmid Miniprep Kit, Thermo Scientific). Primers encoding 8-bp 5’ and 3’ barcodes were used to amplify samples from each condition.
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bioRxiv - Microbiology 2019Quote: ... purified and normalized using a SequalPrep Normalization Plate (96) Kit (ThermoFisher Scientific, Waltham, MA). Samples ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted in 96-well plates using the PureLink DNA Extraction Kit (Invitrogen), using instructions for gram-positive bacteria ...
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bioRxiv - Genetics 2022Quote: ... SequalPrep™ Normalization Plate Kit was used to normalize the amplicon concentration (Thermofisher Scientific). Sequencing was done using the Illumina MiSeq Reagent Kit V2 with V4 sequencing primers as described by Caporaso et al (2012 ...
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bioRxiv - Microbiology 2022Quote: ... Purification and normalization of amplicons were performed using a SequalPrep plate kit (Invitrogen, USA), and the DNA concentration was measured with a Qubit® Fluorometer ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were cleaned and normalized using SequalPrep Normalization Plate Kit (Thermo Fisher Scientific), then quantified and pooled equimolarly according to Kapa Biosystems Library qPCR MasterMix (ROX Low ...
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bioRxiv - Microbiology 2023Quote: ... Barcoded amplicons were again purified and normalized with the SequalPrep Normalization Plate Kit (Invitrogen), pooled ...