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Citations for Thermo Fisher :
951 - 1000 of 5300 citations for Octreotide acetate impurity H since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... contrast stained for 10 min with 2% uranyl acetate and visualized in a TEM (L120C, Talos, Thermo Fisher Scientific, MA, USA).
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was eluted in 50 μl water and precipitated with 0.75 M ammonium-acetate and 10 μg glycogen (ThermoFisher, Cat# R0551) by adding 125 μl ethanol (100 %) ...
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bioRxiv - Microbiology 2022Quote: ... Stool supernatant from the primary collection were obtained by centrifugation and filtration (0.2 μM surfactant-free cellulose acetate; Thermo Scientific Nalgene). Proteins were precipitated (1:7 ratio (v/v ...
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bioRxiv - Immunology 2022Quote: ... THP1 cells were differentiated into macrophage-like cells by treatment with 10nM phorbol 12-myristate 13-acetate (PMA) for a period of 72h. Lipofectamine2000 (cat no. #11668019) from Thermo Fisher Scientific or Helix-IN reagent (cat no ...
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bioRxiv - Cell Biology 2023Quote: ... stained with uranyl acetate and Sato’s lead solutions and observed in a Transmission Electron Microscope Talos L120C (FEI, Thermo Fisher Scientific) operating at 120kV ...
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bioRxiv - Molecular Biology 2024Quote: ... extracts with high percentages of ssDNA were analyzed on denaturing gels (1 M urea, 0.8% agarose, 1x Tris-acetate-EDTA (TAE) buffer and 1x SybrGold (Thermo Fisher Scientific)) at 50 V for 3 hours ...
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bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.
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bioRxiv - Evolutionary Biology 2024Quote: ... of the homogenized protein-loading buffer sample were loaded in duplicates on a NuPAGE™ 3-8% Tris-Acetate gel (ThermoFisher) to be probed separately with FLAG and ATPase antibodies ...
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bioRxiv - Genomics 2024Quote: ... Protein extracts were subjected to electrophoresis in 10% polyacrylamide SDS gels using Tris-glycine-SDS running buffer (Alaos) or in NuPAGE 3-8% Tris-acetate precast polyacrylamide gels (Thermo Fisher), with Tris-acetate SDS running buffer (Thermo Fisher) ...
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bioRxiv - Biochemistry 2023Quote: ... stained with uranyl acetate and Sato’s lead solutions and observed in a Transmission Electron Microscope Talos L120C (FEI, Thermo Fisher Scientific) operating at 120kV.
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bioRxiv - Molecular Biology 2023Quote: DNA illustration by agarose gel electrophoresis has been conducted by exploiting 2% agarose gel and 1X TAE buffer (1 mM EDTA and 40 mM Tris-acetate, Fisher Scientific) and by applying constant voltage of 120 V for 40 to 60 min ...
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bioRxiv - Molecular Biology 2023Quote: ... 10μg of total protein were heat-denatured for 5 minutes at 100°C before loading onto NuPAGE 3– 8% TRIS Acetate Midi Gel (Novex, Life Technologies) and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Microbiology 2023Quote: ... proteins were crashed out by addition of 300 µl ethyl acetate and all samples were evaporated to dryness in a SpeedVac vacuum concentrator (SPD131DDA SpeedVac Concentrator; Thermo Scientific). Dried samples were stored at −20C until further processing ...
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bioRxiv - Neuroscience 2023Quote: ... all samples were buffer exchanged to 200 mM ammonium acetate with Zeba™ spin desalting columns (Thermo Fisher Scientific, Waltham, MA), with an approximate protein concentration of 2-10 µM ...
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bioRxiv - Microbiology 2023Quote: High-performance LC grade solvents (water, acetonitrile, chloroform, and methanol) and ammonium acetate (Optima LC/MS grade) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Cultures were stimulated with phorbol 12-myristate 13-acetate (PMA) (5 ng/mL, Alfa-Aesar) + Ionomycin (0.5 µg/mL, Thermo Fisher Scientific) and supplemented with sCTLA-4 conditioned media or murine CTLA-4-Fc fusion protein (10 µg/mL ...
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bioRxiv - Genetics 2023Quote: ... the denatured nuclear extract was subjected to electrophoresis on a precast 3-8% Tris-Acetate polyacrylamide gel (Thermo Fisher Scientific, EA0378BOX) in 1× Tris-Acetate SDS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: Chlamydomonas reinhardtii strains were maintained on solid Tris-Acetate-Phosphate (TAP) media containing 1.6% [w/v] agar (USP grade, Thermo Fisher Scientific) and Hutner’s trace elements (64 ...
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bioRxiv - Cell Biology 2023Quote: ... lysates were separated by sodium dodecyl sulfate/polyacrylamide (SDS/PAGE) gel electrophoresis on 1 mm NuPAGE 3 – 8% gradient Tris-Acetate gels (Thermo Fisher) using Tris-Acetate SDS running buffer and transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Biophysics 2023Quote: The samples were then buffer exchanged into either 300 mM or 500 mM ammonium acetate solution for the nucleotidylation or deRNAylation samples respectively using Zeba microspin desalting columns with a 40-kDa MWCO (Thermo Scientific). The nMS solutions contained 0.01% Tween-2045 ...
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bioRxiv - Neuroscience 2024Quote: ... stained with uranyl acetate and Sato’s lead solutions and observed in a Transmission Electron Microscope Talos L120C (FEI, Thermo Fisher Scientific) operating at 120kV ...
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bioRxiv - Cell Biology 2024Quote: ... filled with 100 μl of water and 20 μl of 3 N aqueous sodium acetate and purified by chloroform/phenol (Life Technologies) extractions ...
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bioRxiv - Molecular Biology 2024Quote: The 1A/3xFLAG-MYCN complex was desalted into 200 mM ammonium acetate using 75 μl Zeba spin desalting columns (ThermoFisher Scientific). Samples were analysed by nanoelectrospray ionisation MS using a quadrupole-orbitrap MS (Q-Exactive UHMR ...
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bioRxiv - Plant Biology 2024Quote: ... 48 and 72 hours of incubation with and without CTec3 were also analyzed for acetate contents using a Dionex Ultimate 3000 system (Thermo Scientific) with a Rezex refractive index detector and a ROA-organic acid H+(8 %) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was flash-frozen in pH 7.5 buffer or buffer exchanged into “lysosomal pH buffer” (25 mM sodium acetate pH 4.5, 150 mM NaCl) using a Zeba 7k MWCO spin column (Thermo Scientific, PI89882) according to manufacturer instructions and diluted to a final concentration of 1 mg/mL.
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bioRxiv - Immunology 2024Quote: ... freshly isolated neutrophils cell suspension either in Eppendorf tubes or plated on P-selectin-coated plates were activated using 100nM phorbol myristate acetate (PMA) (Fisher Scientific), 5µg/L tumor necrosis factor-α (TNF-α ...
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bioRxiv - Genetics 2024Quote: ... and 10 µg/lane protein extracts were submitted to 3–8 percent NuPAGE Novex Tris-acetate gel electrophoresis (Thermo Fisher Scientific) with HiMark prestained standard proteins (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa Fluor® 488 goat anti-rabbit IgG (H+L) and Alexa Fluor® 555 goat anti-rabbit IgG (H+L) (Life Technologies, Carlsbad, California, USA).
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bioRxiv - Cancer Biology 2021Quote: ... goat anti-mouse IgM (H) Alexa Fluor 594 (Thermo Fisher Scientific) or goat anti-mouse IgG Alexa Fluor 647 (for CALR mutant ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-rabbit Alexa 568 IgG (H+L) (1:100; Invitrogen), goat anti-mouse Atto 647N (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-Chicken IgY (H+L) Alexa-488 (Thermofisher A-11039) (dilution 1:500) ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor® 488 Goat-anti-Rabbit IgG (H+L) (Invitrogen), Alexa Fluor® 594 Goat-anti-Mouse IgG (H+L ...
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bioRxiv - Genomics 2020Quote: ... cDNA was synthesised using RevertAid H Minus reverse transcriptase (Thermo Scientific) and random hexamer primers (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Goat anti-Mouse IgG (H+L) HRP (1:2000; Invitrogen, A16066), Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and RevertAid H minus RT reverse transcriptase (Thermo Scientific, Life Technologies) at 45 °C.
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bioRxiv - Systems Biology 2022Quote: ... and RevertAid H Minus Reverse Transcriptase (EP0451, Thermo Scientific, Massachusetts, USA), and then used for real-time (rt)-PCR (5× HOT FIREPol EvaGreen qPCR Mix Plus (ROX) ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor 488 goat anti-rat IgG(H+L) (Life Technologies) were added (1:400 ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% BSA) loaded with 5 μM coelenterazine h (Thermo Fisher Scientific) for 4 h to reconstitute the holo-enzyme aequorin ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor 568 donkey anti-rabbit IgG (H+L) (Thermo Fisher Scientific Cat#A10042 ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μl of 5X Maxima H Minus RT Buffer (Thermo Fisher), 1 μl RNaseOUT (ThermoFisher) ...
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bioRxiv - Genomics 2020Quote: ... and blocked for 1 h using 0,2% I-Block (ThermoFisher T2015) with 0,1% Tween20 (Merck ...
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bioRxiv - Systems Biology 2020Quote: ... and 24 μL Maxima H Minus Reverse Transcriptase (EP0753, Thermo Scientific). 3 * 107 cells were added to this mix ...
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bioRxiv - Neuroscience 2020Quote: ... and 0.1 μL Maxima H Minus Reverse Transcriptase (Thermo Scientific, EP0751). Reverse transcription was performed by incubating the samples at 50°C for 30 min and terminated by heating at 85°C for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... stained for 1 h in Alexa 488-phalloidin (Invitrogen, Cat# A12379) diluted to 1:200 in blocking solution ...
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bioRxiv - Neuroscience 2021Quote: ... 0.2 µL of 200 U/µL Maxima H Reverse Transcriptase (ThermoFisher), 0.2 µL of 20 U/µL SUPERaseIN ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM DTT) with 0.4 mg RNase H (Thermo Fisher Scientific), iv ...
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bioRxiv - Cell Biology 2021Quote: ... goat anti-mouse igG (H+L) Alexa Fluor 488 (A11029, Invitrogen) and goat anti-rabbit igG (H+L ...
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bioRxiv - Cell Biology 2021Quote: ... and goat anti-rabbit igG (H+L) Alexa 633 (A21070, Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... One wash included Hoechst 33342 (1:10,000; H-3570; Molecular Probes) to stain nuclei ...