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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... Protein concentration was determined using the BCA assay (Thermo Scientific, Rockford, IL, USA). Mitochondrial respiration of isolated mitochondria was measured within two hours of isolation.
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bioRxiv - Immunology 2022Quote: ... RNA purity and quantity were evaluated with Nanodrop spectrophotometer (Thermo Scientific, Rockford, IL). cDNA synthesis was performed using EvoScript Universal cDNA Master ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with a protease and phosphatase inhibitor cocktail (Thermo Scientific, Rockford, IL, USA) using a Potter-homogenizator ...
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bioRxiv - Molecular Biology 2021Quote: ... and 50 ng/ml human recombinant M-CSF (ThermoFisher Scientific, Rockford, IL, USA). After six days of PBMC culture ...
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bioRxiv - Immunology 2021Quote: ... and IL-12p70 production were measured using the cytokine kits purchased from Invitrogen and following the instructions from the manufacturer.
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bioRxiv - Cell Biology 2020Quote: ... and murine IL-6 were measured using commercial ELISA kits obtained from Invitrogen, Thermo Fisher Scientific (Lower detection limits ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mM NaF) containing Halt protease cocktail inhibitor (Thermo Scientific, Rockford, IL, USA). Following ten minutes incubation on ice ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentrations were determined by BCA protein assay (Thermo-Fisher Scientific, Rockford, IL). Protein samples were reduced with 10 mM TCEP and alkylated with 25 mM iodoacetamide before trypsin digestion in 1/40 enzyme/protein ratio at 37°C overnight.
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bioRxiv - Neuroscience 2020Quote: ... Bicinchoninic Acid (BCA) protein assay kit was obtained from Thermo Scientific (Rockford, IL). ProSignal Blotting Film was purchased from Genesee Scientific (El Cajon ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The ELISA was performed using Human IL-1β high sensitivity kit from Invitrogen Inc ...
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bioRxiv - Biophysics 2020Quote: ... and stimulated with 50 U/mL recombinant interleukin-2 (IL-2, Thermo Fisher) and 5 μg/mL phytohaemagglutinin (PHA ...
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bioRxiv - Cancer Biology 2021Quote: ... The same medium with addition of 10ng/ml IL-6 (Gibco, Ref: PHC0066) was used for primary MM plasma cell cultures ...
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bioRxiv - Cell Biology 2021Quote: ... Pierce™ Anti-HA Agarose (26181) were purchased from Thermo Scientific (Rockford, IL). Deubiquitinases (DUBs ...
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bioRxiv - Microbiology 2021Quote: Quantification of IL-1β secretion in cell supernatant was measured by ELISA (Invitrogen, IL-1 beta Mouse Uncoated ELISA Kit ...
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bioRxiv - Microbiology 2021Quote: ... Tryptic peptides were further desalted using C18 spin columns (Thermo Fisher Scientific, IL). Samples were then lyophilized and reconstituted in 0.1% trifluoroacetic acid to be analyzed in quadruplicates without fractionation for quantitation purposes.
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentration was measured (Pierce BCA Protein Assay, Thermo Fisher Scientific, IL, USA) and a total of 100 µg of protein per sample was subjected for trypsin digestion ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized by chemiluminescence (Pierce Supersignal West Dura, Thermo Scientific, IL, USA) and quantified with ChemiDocTM MP imaging system (BioRad) ...
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bioRxiv - Bioengineering 2020Quote: ... and purified using the MEGAclear Transcription Clean-Up kit (Thermo Fisher, Chicago, IL) as described by Vilarino et al10 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with protease and phosphatase inhibitor cocktail (PPI; Thermo Scientific, Rockford, IL, USA) and EDTA (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... USA)[49] and 1 μM Höchst 33342 (Thermo Fisher Scientific Inc, Rockford, IL, USA) in PBS and immediately imaged using a Zeiss Imager (larval FB ...
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bioRxiv - Immunology 2022Quote: ... IFN-γ and IL-17A were measured with uncoated ELISA kits from Invitrogen in accordance with manufacturer’s recommendations.
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bioRxiv - Immunology 2022Quote: ... Proteins were denatured using NuPAGE LDS Sample Buffer (Thermo Fisher Scientific, Rockford, IL). The proteins were resolved by SDS-PAGE on 4-12% NuPAGE gels using NuPAGE MOPS buffer (NuPage gels ...
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bioRxiv - Cell Biology 2021Quote: ... The blots were visualized using SuperSignal-enhanced chemiluminescence (Thermo Scientific, Rockfold, IL, USA) and chemiluminescence function on a GE Healthcare Amersham AI 600 RGB imager ...
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bioRxiv - Immunology 2021Quote: ... 100 μL of IL-2 standard (Invitrogen eBioscience, catalog no. 14-8021-64) or supernatants are added to the wells ...
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bioRxiv - Physiology 2021Quote: ... pH – 7.5) with phosphatase and protease inhibitors (HALT Thermo Scientific, Rockford, IL, USA) using a tissue homogenizer (Bullet Blender ...
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bioRxiv - Immunology 2020Quote: ... IgM (Il/41-PE-Cy7, eBioscience) and live/dead Near-IR (Life Technologies). A secondary staining step was performed using streptavidin-BUV395 (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... Rabbit anti-SARS-CoV nucleocapsid polyclonal antibody (PA1-41098, Thermo Scientific, Rockford, IL) diluted 1:2,000 in blocking buffer was allowed to bind overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... and counterstained with Richard-Allan Scientific Modified Mayer’s Hematoxylin (Thermo Scientific, Rockford, IL) and 0.2M lithium carbonate (Sigma ...
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bioRxiv - Immunology 2022Quote: ... Maturation was induced by a combination of IL-1β (10 ng/ml; Invitrogen), IL-6 (100 ng/ml ...
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bioRxiv - Immunology 2019Quote: ... and IL-10 were analyzed by ELISA as described by the manufacturer (ThermoFisher).
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bioRxiv - Cell Biology 2019Quote: ... and were imaged in FluoroBrite™ DMEM media (Thermo Fisher Scientific, Rockford, IL) containing 5% FBS and 20 mM HEPES at 37°C ...
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bioRxiv - Immunology 2019Quote: ... emulsified in 2 mg alum (Imject™ Alum, Thermo Fisher Scientific, IL, USA). Non-allergic control animals (PBS ...
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bioRxiv - Microbiology 2019Quote: ... Protein was quantitated by bicinchoninic acid assay (Thermo Scientific Pierce, Rockford, IL, USA) and analyzed with SDS-PAGE ...
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bioRxiv - Developmental Biology 2021Quote: ... and collected in Superfrost® Plus slides (Thermo Fisher Scientific, Rockford, IL, USA). For laser microdissections ...
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bioRxiv - Microbiology 2020Quote: ... and anti-IL-2 (No. 25-7021; eBioscience/Affymetrix, Santa Clara, CA, USA) antibodies at room temperature for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was measured (Pierce BCA Protein Assay, Thermo Fisher Scientific, IL, USA) and a total of 100 μg of protein per sample was subjected for overnight trypsin digestion ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and integrity were evaluated with a Nanodrop 2000 spectrophotometer (Thermo Scientific, IL, USA), Qubit 2.0 Fluorometer together with the DNA High Sensitivity kit (Life Technologies ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The ELISA was performed using Human IL-1β high sensitivity kit from Invitrogen. The sample of ELISA used in the present study consist of cell culture media (referred to as sample hereafter ...
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bioRxiv - Neuroscience 2020Quote: Relative transcript levels of the inflammatory cytokines IL-1ß (ThermoFisher Scientific, Cat. # Mm00434228_m1), and TNFα (Cat ...
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bioRxiv - Cancer Biology 2021Quote: ... and Streptavidin Alexa Fluor 488 conjugate were purchased from Thermo Scientific (Rockford, IL). Aniline was from BeanTown Chemical Corporation (Hudson ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM EDTA and Halt Protease inhibitor cocktail (Thermo Scientific, Rockford, IL, USA). The cell lysates were fractionated using 4–12% NuPAGE gels (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... and 30 U/ml of interleukin-2 (IL-2; Thermo Fisher Scientific, PHC0026) at 37°C ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... Blots were incubated with SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific, Rockford, IL) and exposed to Amersham Hyperfilm ELC (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... and IL-13 Alexa Fluor 488 (ThermoFisher, cat. 53-7133-82, clone eBio13A). Finally ...
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bioRxiv - Genomics 2022Quote: ... DNA quality was assessed with a Nanodrop 2000 spectrophotometer (Thermo Scientific, IL, USA), by a Qubit 2.0 Fluorometer with the DNA High Sensitivity kit (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... HRP was activated using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, Rockford, IL) and imaged with a FluorChem Q system (Protein Simple ...
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bioRxiv - Immunology 2022Quote: ... 100 µL of detection antibody (IFNγ, BD Biosciences, #51-1818KA; IL-17A, Invitrogen, #88-7371-88 ...
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bioRxiv - Neuroscience 2022Quote: ... IL-6 (1:4-1:2 dilution, #KMC0061; ThermoFisher Scientific, Waltham, MA, USA), and NfL (1:4 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... and 1% non-essential amino acids (all Thermo Fisher Scientific, Rockford, IL, USA) at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The ELISA was performed using Human IL-1b high sensitivity kit from Invitrogen. The samples for ELISA were media collected after treating the cells with drugs at 100uM ...