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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for Dog Trefoil Factor 3 TFF3 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... The supernatants were loaded into a protein gel NativePAGE Novex 3-12 % Bis-Tris (1.0 mm, 15-well; Thermo Fisher Scientific, catalog number BN2012BX10), following the manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... RNA was extracted from control and CRISPR-Cas9 targeted Calu-3 cells (N = 3 biological replicates, with 3 technical replicates per experiment per condition) and prepared using Trizol Reagent (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... mixed with forward and reverse detection primers (T3DCD S1 forward [5’- TACGCGTTGATCACGACAAT-3’] and T3DCD S1 reverse [5’- TGGCGAGATTATTCCCTGAC-3’] or GAPDH forward [5’- ACCCAGAAGACTGTGGATGG-3’] and GAPDH reverse [5’- GGATGCAGGGATGATGTTCT-3’]) and SYBR Select Master Mix (Applied Biosystems), and then subjected to PCR using the StepOnePlus Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... and customized si-KIF4 3′ UTR targeting endogenous KIF4 mRNA 3’-UTR region (5′-GGAAUGAGGUUGUGAUCUUTT-3′) were purchased from Thermo Fisher Scientific.
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bioRxiv - Cell Biology 2020Quote: ... reverse: 5’-GGGGTCGGGAGGAACGG-3’ (Macrogen, South Korea) and beta-actin forward: 5’-CCTGGTCGGTTTGATGTT-3’ and reverse: 5’-GTGCGACGAAGACGA-3’ (Invitrogen, USA). Data analysis was made in CFX ManagerTM Software (BioRad ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... FW 5’-CTCTCTGGGCGAAATCTGCT-3’ and REV 5’-GAGTGCTTTCGCTATGTTGTTCA-3’ for Clmn and FW 5’-TGATGGGTGTGAACCACGAG-3’ and REV 5’-GCCGTATTCATTGTCATACCAGG-3’ for Gapdh using a QuantStudio 7 (Applied Biosystems). Transcript levels are expressed as the ratio of 2−ΔCT (Transcript of interest)/2−ΔCT (Gapdh).
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bioRxiv - Neuroscience 2022Quote: ... total RNA was extracted from freshly-dissected n=3 young (3-month-old) or n=3 aged (18-month-old) zebrafish brain in TRIzol (Invitrogen, 15596). Three independent experimental replicates were used for bulk RNA sequencing ...
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bioRxiv - Immunology 2020Quote: ... Precipitated proteins were bound to 50:50 Protein A: Protein G Dynabeads (Invitrogen) for one to two hours and washed extensively with IP wash buffer (50 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were incubated with fast-flow protein G- or protein A-Dynabeads (Invitrogen) for 2 hours and centrifuged to eliminate nonspecifically bound proteins ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then fixed and permeabilized with either eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen) or Fixation/Permeabilization Solution Kit (BD Biosciences) ...
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bioRxiv - Cancer Biology 2021Quote: ... with one half fixed and permeabilized with either eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen), and the other half kept alive in PBS with 2% FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed and permeabilized with the FoxP3 transcription factor kit from eBioscience (Thermo Fisher Scientific) and stained for FoxP3 and IFNg according to the manufacturers protocol ...
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bioRxiv - Bioengineering 2021Quote: ... platelet-derived growth factor receptor A (PDGFRA) and type I collagen (COL1A1) (Thermo Fisher, Waltham, Massachusetts). Probe references ...
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bioRxiv - Bioengineering 2021Quote: ... for 30 min then fixed and permeabilized with eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen). Samples were next Fc-blocked for 10 min using TruStain fcX (BioLegend) ...
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bioRxiv - Bioengineering 2020Quote: ... 500 μl crypt culture medium (ENR+CV) containing growth factors EGF (50 ng/ml, Life Technologies), Noggin (100 ng/ml ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration of the purified factors was determined using ND-1000 UV-Vis Spectrophotometer (Thermo Fisher) by measuring absorbance at 280 nm ...
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bioRxiv - Neuroscience 2021Quote: ... 20 ng/ml glial cell-derived neurotrophic factor (GDNF) (all from Thermo Fisher Scientific, Massachusetts, USA), 100 ng/mL cAMP ...
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bioRxiv - Neuroscience 2020Quote: ... The iodinated growth factors were concentrated by using YM-10 Centricon columns (Thermo Scientific, MA, USA). The specific activity of 125I-labeled CDNF was 108 cpm/μg.
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bioRxiv - Neuroscience 2020Quote: ... and human basic Fibroblast Growth Factor (bFGF) (Thermo Fisher Scientific 68-8785-82, 20 ng/mL). LUHMES cells were grown to 80% confluency and passaged 1:6 using diluted trypsin-EDTA (0.25% ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed and permeabilized with the eBioscience FOXP3/Transcription Factor Fixation/Permeabilization buffer set (ThermoFisher) and then subjected to intracellular staining with anti- C1Q (RmC7H8) ...
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bioRxiv - Immunology 2022Quote: ... permeabilized and red cells lysed with the eBioscience Transcription Factor Staining Buffer Kit (Life Technologies/eBioscience) for at least 1hr followed by intracellular antigen staining for 30min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... permeabilized and red cells lysed with the eBioscience Transcription Factor Staining Buffer Kit (Life Technologies/eBioscience) for at least 1hr followed by intracellular antigen staining for 30 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Intracellular staining for transcription factors was performed using the Foxp3 Staining Buffer Set (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... Intracellular staining was performed using the Transcription Factor/FOXP3 Fixation/Permeabilization Solution Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... fixed using the eBioscience Foxp3 Transcription Factor Fixation/Permeabilization Concentrate and Diluent (ThermoFisher Scientific: 00-8222) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... penicillin/streptomycin (1×) and 2.5 ng ml-1 basic fibroblast growth factor (bFGF, 13256, Life Technologies).
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bioRxiv - Immunology 2021Quote: ... Intracellular staining for transcription factors was performed using the Foxp3 staining buffer set (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed and permeabilized using the eBioscience FOXP3/Transcription factor staining buffer set from Invitrogen.
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed and permeated with a transcription factor staining buffer set (Invitrogen 00-5523-00) following staining for extracellular proteins ...
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bioRxiv - Microbiology 2023Quote: ... 10 μl of ice-cold Geltrex LVES-free Reduced Growth Factor Basement Membrane Matrix (ThermoFisher Scientific) was added using pre-chilled pipettes to each well of a chilled μslide ...
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bioRxiv - Biophysics 2023Quote: ... and 5 ng/mL prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53; Gibco). Hippocampal neurons were grown in neuro basal medium ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were fixed and washed using Transcription factor staining buffer set (00-5523-00, ThermoFisher Scientific) and permeabilization buffer according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed and permeabilized with the FoxP3 transcription factor staining buffer set (Thermo Fisher Scientific) at 4°C for 1h ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 12 ng/mL recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF) (Thermo Fisher Scientific, USA) was included in the culture medium ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed using the eBioscience Foxp3 Transcription Factor Fixation/Permeabilization Concentrate and Diluent (ThermoFisher Scientific) for 20 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... These cells were supplemented with 10ng/ml leukemia inhibitory factor (LIF) and 0.1mM 2-mercaptoethanol (Gibco) to maintains pluripotency ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining was performed using eBioscience™ Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific) for the detection of transcription factors ...
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bioRxiv - Molecular Biology 2024Quote: ... in which the factor was captured and developed by specific antibodies linked to horseradish peroxidase (Invitrogen).
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bioRxiv - Immunology 2024Quote: ... recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) (300U/mL; Thermo Fisher Scientific, Waltham, MA) and recombinant human interleukin 4 (rhIL-4 ...
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bioRxiv - Systems Biology 2023Quote: ... cells were fixed and permeabilized using the eBioscience Foxp3 / Transcription Factor Staining Buffer Set (Thermo Fisher) for 40 minutes at 4°C and then washed in perm/wash buffer at 500g for 4 minutes ...
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bioRxiv - Immunology 2023Quote: ... Intracellular stains (i.e., transcription factors) were performed using 1X permeabilization buffer (Foxp3 fixation/permeabilization Kit, Invitrogen). Samples were acquired on a BD LSRII Fortessa using BD FACSDiva (BD Bioscience) ...
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bioRxiv - Immunology 2023Quote: ... FoxP3 and T-BET was performed using The Foxp3 Transcription Factor Staining Buffer Set (Thermofisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... samples were fixed and permeabilized using the FoxP3 Transcription Factor Staining Buffer Kit (Thermo Fisher Scientific) for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... Cells were then fixed and permeabilized using the Foxp3 Transcription Factor Staining Buffer Set (Thermo Fisher), and subsequently stained for intracellular and intranuclear proteins ...
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bioRxiv - Cell Biology 2022Quote: ... the growth medium of cells was replaced with growth factor-free medium consisting of DMEM (Gibco) supplemented with 4.76 mg/mL HEPES and 3 mg/mL bovine serum albumin ...
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bioRxiv - Microbiology 2022Quote: ... PFA-fixed cells were washed and permeabilized with eBioscience FoxP3/Transcription Factor Staining Buffer (Thermo Fisher) for 10min at 4C ...
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bioRxiv - Immunology 2022Quote: ... cells were fixed and permeabilized using Foxp3 / Transcription Factor Staining Buffer Set (#00-5523-00, Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Sendai viral particle factors from CytoTune-iPS Sendai Reprogramming Kit (A16518, Life Technologies, Carlsbad, CA, USA) were added based on the manufacturer’s recommendations54 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Culture plates were coated with Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Gibco #A1413201). Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12 ...
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bioRxiv - Neuroscience 2023Quote: ... and human heat stable basic Fibroblast Growth Factor (bFGF) (Thermo Fisher Scientific PHG0369; 20 ng/ml) in vessels pre-coated with poly-L-ornithine hydrobromide (Sigma-Aldrich P3655 ...