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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for Cytosolic arginine sensor for mTORC1 subunit 2 CASTOR2 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... purified 40S-RACK1-ybbR subunits were incubated with 4-fold molar excess PEG11-biotin (ThermoFisher #21911) conjugated to co-enzyme A (CoA ...
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bioRxiv - Neuroscience 2021Quote: ... A 200-400 nl volume of 0.1% AlexaFluor647-conjugate Cholera Toxin subunit b (CTB, Thermofisher Scientific) was delivered using a glass pipette pulled from borosilicate glass (3.5” 3-000-203-G/X ...
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bioRxiv - Neuroscience 2021Quote: ... Cholera toxin subunit-b (conjugated to AlexaFluor 555 (CTB-555; 500nl, 1 mg/ml; ThermoFisher Scientific) was injected into one of two major projection targets of S1 (n = 6 mice for each projection target) ...
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bioRxiv - Neuroscience 2022Quote: ... or 3) injections of fluorophore-conjugated cholera toxin subunit B (CTB 488 or CTB 555; Invitrogen). These manipulations were all targeted to A2 ...
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bioRxiv - Physiology 2024Quote: ... and γ subunits and mouse prostasin were transcribed using the mMessage mMachine transcription kit (Life Technologies). Resulting cRNA encoding each of the subunits was injected into stage V to VI Xenopus oocytes ...
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bioRxiv - Neuroscience 2023Quote: ... or mouse anti-OxPhos Complex IV subunit IV (anti-COX IV; A-21347, Thermo Fisher Scientific) diluted 1:1000 in TBS-T with 5% Bovine Serum Albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... 200uL of Cholera toxin subunit B (CTB) conjugated to Alexa Fluor 555 (Life technologies, Cat# C34776) or Alexa Fluor 647 (Life technologies ...
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bioRxiv - Neuroscience 2023Quote: Injections of cholera toxin subunit b conjugated to Alexa Fluor 555 (CTB-555) (Thermo Scientific C34776) was injected at 1X 150-200nl in the secondary motor cortex as described above ...
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bioRxiv - Neuroscience 2024Quote: ... 100 μg of recombinant cholera toxin subunit B conjugated to Alexa Fluor 488 (CTb-AF488; ThermoFisher) was dissolved in 500 μl of 1 M PBS (pH=7.4 ...
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bioRxiv - Immunology 2021Quote: ... Membranes were blocked with 5% BSA in TBST buffer and probed with anti-mouse Tim-3 goat polyclonal antibody (R&D) and detected with anti-goat-HRP antibody and SuperSignal West Pico Chemiluminescent reagent (Thermo Fisher).
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bioRxiv - Immunology 2021Quote: ... and human transferrin was used as a loading control and detected using transferrin antibodies from chicken and chicken-HRP conjugated antibodies (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Expression of ectopic proteins was confirmed via western blotting with FHIP1B and FHIP2A-specific antibodies as well as anti-V5-HRP antibody (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... the bound antibodies were detected by incubation with horseradish peroxidase (HRP)-conjugated anti-mouse IgG antibody (1:5,000, Thermo Fisher Scientific) for 1 h at 37°C ...
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bioRxiv - Systems Biology 2020Quote: ... Visualization of antibody signals was achieved using HRP-conjugated secondary antibodies and the chemiluminescence substrate SuperSignal West Pico (Thermo Fisher Scientific). Blots were imaged and quantitatively analyzed using a Chemidoc MP imager (Biorad) ...
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bioRxiv - Microbiology 2020Quote: ... The resulting blots were probed with a sdAb as primary antibody and an HRP-linked 6x-His Tag antibody (Thermofisher, HIS.H8) as the secondary antibody ...
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bioRxiv - Microbiology 2021Quote: ... or rabbit TgALD1 antibody (1:200, kind gift from Dr. Kentaro Kato) and anti-rabbit HRP antibodies (Thermo Fisher, 1:10000) followed by development of signal with West Pico Plus Chemiluminescent Substrate (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: Viral N protein was detected using a rabbit anti-SARS-CoV N antibody [32] as a primary antibody with AlexaFluor 568 anti-rabbit IgG or anti-rabbit IgG-HRP (Thermo Fisher) as secondary antibodies together with DAPI to stain the nucleus by indirect immunofluorescence as described previously [33].
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bioRxiv - Cell Biology 2021Quote: ... and subjected to primary antibody and HRP conjugated secondary antibody before visualization with Pierce™ ECL Western Blotting Substrate (Thermo Fisher). Western blot images were obtained using the ChemiDoc Imager (BioRad).
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bioRxiv - Microbiology 2023Quote: ... probed with antibodies against different targets followed by HRP-conjugated anti-mouse or anti-rabbit IgG secondary antibodies (Thermo Fisher Scientific), and then the protein bands were visualized using enhanced chemiluminescent (ECL ...
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bioRxiv - Bioengineering 2023Quote: ... Primary antibodies were detected by a horseradish peroxidase (HRP)-conjugated anti-rabbit antibody using an ECL kit (Thermo Fisher Scientific, US) and imaged using ChemiDoc TM Touch Imaging System (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... Bound primary antibodies were detected with HRP-conjugated secondary antibody and chemiluminescence assay (SuperSignalTM West Pico PLUS Chemiluminescent Substrate, Thermo Scientific). Total and di-phospho MPK-1B and alpha-tubulin levels were quantified by measuring the corresponding band intensities using built-in measurement tools of Fiji [70] ...
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bioRxiv - Developmental Biology 2023Quote: ... Bound primary antibodies were detected with HRP-conjugated secondary antibodies followed by a chemiluminescence assay (SuperSignalTM West Dura Extended Duration Substrate, Thermo Scientific). To inactivate HRP-conjugates and proceed with the incubation of the primary anti-alpha tubulin antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... Detection of all other primary antibodies was performed using goat anti-rabbit Poly HRP secondary antibody (Invitrogen B40962; 10 min incubation). The HRP-conjugated secondary antibody polymer was detected using fluorescent tyramide signal amplification using Opal dyes 520 ...
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bioRxiv - Molecular Biology 2023Quote: ... Then the samples underwent phosphate buffered saline (PBS) washing for 15min before incubated with the second antibody HRP goat anti-rabbit antibody (ThermoFisher, 31460) which was dissolved into 1% BSA for 1h in room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Visualization of the primary antibody was performed using HRP-conjugated goat anti-rabbit secondary antibody (Thermo Scientific #31460; 1:10,000 dilution) or HRP-conjugated goat anti-mouse secondary antibody (Thermo scientific #31430 ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were washed and then incubated with the corresponding secondary antibodies for 1h at room temperature (Goat Anti-rabbit Secondary Antibody, HRP conjugate 1:1000, ThermoFisher #31460). All antibodies were diluted in a 5% BSA solution in TBS-T ...
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bioRxiv - Microbiology 2023Quote: ... were added as the primary antibodies and HRP-labelled goat anti-human IgE antibody (at 1:1,000 dilution, Invitrogen, Waltham, MA) was used as the secondary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... Foci development was stopped by fixation with 4% formaldehyde and foci were then stained using a mouse-anti-NS1 antibody (1A5) (gift from Jacob Schlesinger, Rochester University) and a horseradish peroxidase (HRP) conjugated secondary anti-mouse antibody (ThermoFisher 31430). The foci were visualized by diaminobenzidine (DAB ...
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bioRxiv - Cancer Biology 2023Quote: ... incubated overnight with primary antibodies at 4 degrees and detected with goat anti-rabbit or mouse-HRP secondary antibodies (Thermo Scientific) followed by detection using SuperSignal™ West Pico Plus chemiluminescent substrate (Thermo Scientific).
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bioRxiv - Immunology 2024Quote: ... Chemiluminescence was used to detect the secondary antibody (Anti-rabbit IgG, HRP-linked Antibody #7074 and SuperSignal™ West Pico PLUS Chemiluminescent Substrate, ThermoFisher).
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bioRxiv - Cancer Biology 2024Quote: ... 1:500 anti-Mouse IgG-HRP-linked whole ab sheep antibody (Cytiva NA931V) and 1:500 anti-rabbit IgG-Alexa 488-conjugated donkey antibody (Invitrogen A21206), (2 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:500 anti-Mouse IgG-HRP-linked whole ab sheep antibody (Cytiva NA931V) and 1:500 anti-rabbit IgG-Alexa 647-conjugated donkey antibody (Invitrogen A31573). Washed three times for 30 minutes each in TNTT at 4 ℃ to remove secondary antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... Appropriate primary antibodies and HRP-conjugated secondary antibodies were used and proteins were detected using the Enhanced Chemiluminescent (ECL) reagent (Thermo Scientific). The images were acquired with ChemiDoc MP System (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... antibodies coupled with the use of an anti-mouse secondary antibody conjugated to HRP (Thermo Fisher Scientific; Cat No 62-6520).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Sections were rinsed with PBS and incubated with secondary antibody HRP-conjugated Goat anti-rabbit secondary antibodies (Invitrogen, 31460, 1:500) for 1 hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Sections were rinsed with PBS and incubated with secondary antibody HRP-conjugated Goat anti-rabbit secondary antibodies (Invitrogen, 31460, 1:500) for 1 hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Sections were rinsed with PBS and incubated with secondary antibody HRP-conjugated Goat anti-rabbit secondary antibodies (Invitrogen, 31460, 1:500) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... 90 µL of supernatant was mixed with 410 µL of IP Dilution Buffer and antibodies (Positive antibody: 10 µL; Negative antibody: 2 µL; FoxO3a antibody: 8 µL (Cat. #720128, Invitrogen)) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: Conditioned medium was obtained by culturing CECs in the arginine-free RPMI-medium (SILAC RPMI-medium, Thermo Fisher Scientific) supplemented with 150 µM ʟ-arginine at the density 1×106 cells/ml for 24h ...
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bioRxiv - Neuroscience 2020Quote: CGN WT and KO cultures (1.5-1.7×106 cells per culture) were grown in custom-made lysine and arginine free NB (Life technologies) to which “medium” (M ...
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bioRxiv - Molecular Biology 2024Quote: ... and LETIILSK were selected for synthesis (AQUA QuantProHeavy peptides with 13C15N-labeled C-terminal lysines or arginines, Thermo Scientific). The optimized LC-MS settings for these eight peptides can be found in Supp ...
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bioRxiv - Synthetic Biology 2024Quote: ... was incubated at 30°C for 8 h in a customized PURE system in which Lys and Arg were replaced with stable isotope amino acids (0.36 mM 13C6 L-lysine-2HCl, 0.36 mM 13C6, 15N4 L-arginine-HCl) (Thermo Scientific) to express aaRS ...
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bioRxiv - Bioengineering 2024Quote: ... both sensors expressed in the cells with live cell imaging solution with 10mM glucose (LCIS+, Gibco, A14291DJ) were imaged using an epifluorescence microscope ...
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bioRxiv - Biochemistry 2023Quote: ... wild-type α-actin and 15 μM Pi-sensor (MDCC-labeled phosphate binding protein, Thermo Fisher Scientific) was prepared ...
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bioRxiv - Biochemistry 2024Quote: Single turnover RasGAP kinetics assays were performed using the Phosphate Sensor system62,63 to detect inorganic phosphate (Invitrogen; ThermoFisher Scientific catalogue number PV4407) ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed with 4% PFA for 30 minutes and stained for intracellular ENV with 1A1D-2 (1/500) monoclonal antibody and anti-mouse 647-conjugated antibody (2 μg/ml, Invitrogen Cat# A21235).
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bioRxiv - Developmental Biology 2022Quote: ... Nuclear and cytosolic fractions of iPSCs-differentiated cells were acquired using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific #78833) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... we introduced a stop codon in the pLRB302 plasmid at residue 640 of the envelope ORF (C640Stop) located at the N’ terminal of the cytosolic tail using the Phusion Site-Directed Mutagenesis Kit (Thermo Fisher Scientific) and the following primers ...
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bioRxiv - Immunology 2020Quote: ... and soluble cytosolic protein (SCP) fractions were extracted from Chinese hamster ovary (CHO) cells and biotinylated using NHS-LC-Biotin (Thermo Fisher Scientific) reagent ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations of both the cytosolic and membrane fractions were measured using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) and equal amounts of protein were loaded on to a NuPAGE 4-12% Bis-Tris gel (Invitrogen) ...