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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for Cytosolic arginine sensor for mTORC1 subunit 2 CASTOR2 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... we used horseradish peroxidase (HRP)-conjugated secondaries recognizing the host species of the primary antibody (Thermo Fisher Scientific 31460 and 31430 for rabbit and mouse respectively) ...
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bioRxiv - Physiology 2024Quote: ... Blots were washed 3×15 min in TBS-T and incubated with HRP-coupled secondary antibodies (ThermoFisher) diluted in 5% milk in TBS-T for 1h at RT ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... membranes were incubated with secondary antibodies (Anti-rabbit, - mouse or -goat IgG, HRP-linked Ab, Thermo Fisher) and visualized using chemiluminescence substrate (SuperSignal West Pico PLUS ...
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bioRxiv - Neuroscience 2021Quote: Intraocular injection of cholera toxin subunit B (CTB) conjugated to Alexa Fluor 488 or Alexa Fluor 555 (Invitrogen) was performed as described previously (66 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Mutations introduced into the δ-subunit were established as stable HEK293 Flp-InTM cell lines (Invitrogen, Paisley, UK), generating a stable cell line for each mutant ...
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bioRxiv - Neuroscience 2021Quote: ... animals were injected with fluorescent cholera toxin subunit B (CTb-AlexaFluor488, CTb-AlexaFluor594; ThermoFisher, C34775 and C34777, respectively) into AcbSh and AcbC (counterbalanced).
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bioRxiv - Immunology 2020Quote: ... S1 or S2 subunit and the final reaction was measured using the Varioskan Lux Reader (Thermo Fisher Scientific) at a wavelength of 405 nm ...
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bioRxiv - Biochemistry 2022Quote: ... baculoviruses harboring yCAF-1 subunits and mutants were made using the Gateway technology subcloning system (Thermo Fisher Scientific). The pDONR/Zeo plasmid containing the sequence encoding Cac1 subunit (Cac1 ...
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bioRxiv - Cell Biology 2023Quote: ... mRNA encoding the TALE nuclease subunits was synthesized using the mMESSAGE mMACHINESP6Transcription Kit (AM1340; ThermoFisher Scientific, Waltham, MA) and purified using RNeasy Mini Kit (74104 ...
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bioRxiv - Neuroscience 2022Quote: ... which encodes the GluN2C protein subunit (Silencer™ select pre-designed siRNA # 4390771, siRNA ID s127815, ThermoFisher Scientific) and a nonactive RNA sequence (Silencer™ select negative control #4390844 ...
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bioRxiv - Biophysics 2024Quote: ... while the N-terminal 6×His-tagged wild-type human Gβ1 and untagged Gγ2 subunits were cloned into a pFastBac-Dual vector (Invitrogen). Recombinant baculoviruses were generated using the Bac-to-Bac® Baculovirus Expression System (Invitrogen) ...
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Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1bioRxiv - Immunology 2022Quote: ... ICAM-2 rabbit monoclonal antibody was bought from Invitrogen (MA5029335) (WB 1:500) ...
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bioRxiv - Genomics 2020Quote: ... and then a 2 hr incubation in secondary antibodies (Invitrogen) at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse monoclonal anti-HA antibody (2-2.2.14, Thermo Fisher, #26183) diluted 1:104 ...
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bioRxiv - Genomics 2021Quote: ... and then a 2 hr incubation in secondary antibodies (Invitrogen) at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... 2 µL of αBb antibody (Invitrogen: PA1-73004; RRID: AB_1016668) was added to the homogenate ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 hours with secondary antibody (Molecular Probes) in PBS and subsequently washed three times in PBS for 10 min (all at room temperature) ...
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bioRxiv - Microbiology 2024Quote: ... SARS-CoV-2 S1 protein primary antibody (Thermo Fisher Scientific) at 1µg/ml were added for 1 h at 37°C ...
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bioRxiv - Immunology 2023Quote: ... 2) Secondary antibodies: anti-mouse-488 (1:1000, A11029, Invitrogen), anti-rabbit-488 (1:1000 ...
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bioRxiv - Physiology 2023Quote: ... then with 2 µg/mL AlexaFluor 594 secondary antibody (Invitrogen). Samples were imaged using a Leica Stellaris confocal microscope.
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bioRxiv - Neuroscience 2023Quote: ... Appropriate goat secondary antibodies (1-2 µg/ml) from ThermoFisher Scientific and Jackson ImmunoResearch were used to immunodetect primary antibodies.
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bioRxiv - Cancer Biology 2024Quote: ... then incubated in secondary antibodies for 2 hours (ThermoFisher Scientific). Primary and secondary antibodies were diluted in 1X TBS with 0.3% Triton and 1% normal horse serum ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated in 10 mL of 3% BSA/TBST (w/v) with 2 µL streptavidin-HRP (1:5,000; S911, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... or 13C6 L-Lysine-2HCl (Lys-8) and 13C6 L-Arginine-Hcl (Arg-10) for the metabolic incorporation of ‘light’ and ‘heavy’ stable isotopes respectively (ThermoFisher Scientific). HEK293FT cells were grown in light or heavy SILAC media for at least 10 doublings ...
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bioRxiv - Synthetic Biology 2021Quote: ... were directly added to yield 50 μL PURE system reaction mixtures [28] containing 13C6 15N4 L-Arginine (R*) and 13C6 L-Lysine (K*) (Thermo Scientific) as a substitute for non-labeled L-Arginine and L-Lysine ...
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bioRxiv - Cell Biology 2022Quote: ... transferred in media containing either low arginine concentrations (1%) or fully reconstituted supplemented with 10% dialyzed FBS (Gibco cat. 26400-044) (50×106 cells/condition ...
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bioRxiv - Systems Biology 2020Quote: ... SILAC DMEM medium (Thermo #88364) lacking L-arginine and L-lysine was firstly supplemented with 10% dialyzed FBS (Thermo Fisher, # 26400044) and the same penicillin/streptomycin mix ...
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bioRxiv - Systems Biology 2020Quote: G9a KO and control Raw 264.7 cells were cultured in AACT/SILAC DMEM medium supplemented with regular lysine and arginine (K0R0) and 10% dialyzed fetal bovine serum (Thermo Fisher), 1% penicillin ...
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bioRxiv - Cell Biology 2020Quote: Stable HA-GLUT4-GFP-expressing 3T3-L1 preadipocytes were grown for 5 doublings and differentiated in Lysine (LYS) and Arginine (ARG)-deficient DMEM (89985; Thermo Scientific), supplemented with 10% dialyzed FBS ...
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bioRxiv - Immunology 2020Quote: ... and 105.8 mg/mL L-Arginine U-13C6,U-15N2 R10 (CKGAS, #CNLM-539-H-0.25)) prepared in RPMI SILAC media (Thermo Scientific, #88365) supplemented with 10% dialyzed FBS (HyClone ...
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bioRxiv - Biochemistry 2023Quote: ... The malonylated synthetic peptide TVDGPSGKmaLWR* containing a stable isotope-labeled arginine residue (R* with 13C6 and 15N4) was obtained from Thermo Fisher Scientific at >90% chemical purity and >99% isotopic purity ...
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bioRxiv - Cell Biology 2024Quote: ... deficient in both L-lysine and L-arginine was supplemented with 1% dialyzed FBS and heavy amino acids 13C615N2 L-Lysine-2HCl and 13C615N4 L-Arginine-HCl (Thermo Scientific) at concentrations of 0.499 mM and 0.699 mM ...
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bioRxiv - Cell Biology 2024Quote: ... deficient in both L-lysine and L-arginine was supplemented with B27 with insulin and heavy amino acids 13C615N2 L-Lysine-2HCl and 13C615N4 L-Arginine-HCl (Thermo Scientific) at concentrations 0.219 mM and 1.149 mM ...
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bioRxiv - Neuroscience 2021Quote: ... anti-rabbit HRP antibody (1:5000) or with anti-goat HRP antibody (1:5000) was performed for 1 h and ECL (Pierce, Thermo Scientific, Rockford, IL, USA) visualized detection ...
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bioRxiv - Microbiology 2020Quote: ... Horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit secondary antibodies were used to detect the primary antibodies (Thermo Scientific, Waltham, MA, USA). After addition of a chemiluminescent HRP substrate (SuperSignal West Pico Chemiluminescent Substrate ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with rabbit antibodies against SFTSV-N as primary antibodies and HRP-conjugated recombinant protein A/G (Cat. No. 32490, Thermo Scientific, Rockford, IL, USA) as secondary antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Visualization of the primary antibody was performed using HRP-conjugated goat anti-mouse secondary antibody (Thermo Scientific 31430; 1:10,000 dilution in TBS-T) and incubation with ECL solution (Merck Millipore WBLUF0500).
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bioRxiv - Cell Biology 2023Quote: ... and blotted with anti-6xHis antibody (1:5000 dilution) followed by HRP-labeled secondary antibody and ECL reagents from the Pierce Fast Western kit (Thermo Scientific, Waltham, MA, USA). The bottom half was stained with SimplyBlue Safe stain (Life Technology ...
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bioRxiv - Neuroscience 2023Quote: ... The blot was probed with the antibody and an HRP conjugated donkey polyclonal anti-mouse IgG secondary antibody (PA1-28664; Invitrogen; Lot # WI3375112, Clone: polyclonal) and a band was observed between 50-37 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... The blot was probed with the antibody and an HRP conjugated donkey polyclonal anti-mouse IgG secondary antibody (PA1-28664; Invitrogen; Lot # WI3375112, Clone: polyclonal) and doublet bands were observed between 75-50 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... The blot was probed with the unconjugated parental antibody and an HRP conjugated donkey polyclonal anti-mouse IgG secondary antibody (PA1-28664; Invitrogen; Lot # WI3375112, Clone: polyclonal) and a band was observed between 100-150 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... The blot was probed with the antibody and an HRP conjugated donkey polyclonal anti-mouse IgG secondary antibody (PA1-28664; Invitrogen; Lot # WI3375112, Clone: polyclonal) and a band was observed between 250-150 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... The blot was probed with the antibody and an HRP conjugated donkey polyclonal anti-mouse IgG secondary antibody (PA1-28664; Invitrogen; Lot # WI3375112, Clone: polyclonal) and a band was observed at between 20 kDa-15 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... The blots were probed with the antibody and an HRP conjugated donkey polyclonal anti-goat IgG secondary antibody (PA1-28664; Invitrogen; Lot # WI3375112, Clone: polyclonal) and a single band was observed between 100 kDa-75 kDa ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were recognized by secondary antibodies either anti-mouse or anti-rabbit IgG HRP-conjugated diluted 1/10000 (Thermo Fisher A11005 and A31556). Blots were developed using the ECL plus western blotting system (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μg nuclear proteins or 7.5 μg cytosolic proteins were boiled in loading buffer with reducing reagents (Invitrogen, NuPAGE™ LDS Sample Buffer (4X), NP0007) ...
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bioRxiv - Cell Biology 2024Quote: Whole retina was fractionated into cytosolic and post-cytosolic (mitochondrial enriched) fractions as previously described (Weh et al., 2020) using the Subcellular Protein Fractionation Kit for Tissues (Thermo Fisher Scientific, Cat # 87790). Both retinas from a single animal were pooled and homogenized with a Dounce homogenizer in cytoplasmic extraction buffer supplemented with protease (HaltTM Protease Inhibitor Cocktail ...
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bioRxiv - Cancer Biology 2020Quote: ... Secondary HRP-anti-rabbit and HRP-anti-mouse were obtained from ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary HRP-anti-rabbit and HRP-anti-mouse were obtained from ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary HRP-anti-rabbit and HRP-anti-mouse were obtained from ThermoFisher Scientific ...