Labshake search
Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for 7 Fluoro 4 hydroxy 3 nitroquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... 7% Fetal bovine serum (FBS)(Cat# 26140079; ThermoFisher Scientific), and 100 U/mL penicillin-streptomycin (Cat# 15140122 ...
-
bioRxiv - Cell Biology 2023Quote: ... Once 1 uL of 7-AAD (Thermo Fisher Scientific) was added ...
-
bioRxiv - Cell Biology 2023Quote: ... Dead cells were stained with 7-AAD (ThermoFisher: A1310) and removed with cell sorter (SONY ...
-
bioRxiv - Microbiology 2023Quote: ... and DSP1-7 plasmids using Lipofectamine 3000 (Thermo Scientific) according to manufacturer’s instructions in triplicates ...
-
bioRxiv - Cancer Biology 2023Quote: ... and Quantstudio 7 flex RT-PCR system (ThermoFisher Scientific). Expression was measured and the ΔCT was calculated ...
-
bioRxiv - Biophysics 2022Quote: COS-7 cells were cultured in DMEM (GIBCO, 21063029) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Plant Biology 2023Quote: ... and ViiA 7 Real-Time PCR machine (Thermofisher, USA) was employed ...
-
bioRxiv - Neuroscience 2024Quote: ... and ran on a QuantStudio 7 Flex (Thermo Fisher). The RNase P TaqMan Copy Number Reference Assay was used for normalization within the same sample ...
-
bioRxiv - Molecular Biology 2023Quote: ... and ViiA-7 Real-Time PCR system (Applied Biosystems). Transcript RNA levels were normalized against β-actin reference gene following the 2-ΔCt method ...
-
bioRxiv - Molecular Biology 2023Quote: ... and ViiA-7 Real-Time PCR system (Applied Biosystems).
-
bioRxiv - Cancer Biology 2023Quote: ... and 7-AAD viability staining solution (Thermo Fisher Scientific), after treatment with a human FcR blocking reagent (Miltenyi Biotec) ...
-
bioRxiv - Cell Biology 2023Quote: ... blocked with 7% goat serum (Gibco; Thermo Fisher Scientific), and incubated with primary antibodies followed by incubation with fluorescent secondary antibodies (Table 2) ...
-
bioRxiv - Immunology 2023Quote: ... 5 μl of 7-AAD (Invitrogen, 00-6993-50) was included for dead cell exclusion.
-
bioRxiv - Genetics 2023Quote: ... A clean glass slide (Fisher Scientific, 12-544-7) was dipped into freshly made PFA solution (1% paraformaldehyde adjusted to pH 9.2 using 1.25 M sodium borate ...
-
bioRxiv - Cell Biology 2024Quote: ... Viability was controlled using 7-Aminoactinomycine D marker (Invitrogen). Fluorescence intensity of CTV and antibodies was measured with the SP6800 cytometer (Sony ...
-
bioRxiv - Cell Biology 2024Quote: ... qPCRs were run on QuantStudio 7 (Applied Biosystems 4485701) and analyzed using the ΔΔCt method ...
-
bioRxiv - Microbiology 2024Quote: ... and 2’,7’-dichlorofluorescein diacetate (Thermo Fisher Scientific, D399) was added at a final concentration of 100 µM ...
-
bioRxiv - Immunology 2024Quote: ... AT270 and HT-7 (Thermo Fisher Scientific, Waltham, MA) were used to capture/detect Tau and pTau181.
-
bioRxiv - Bioengineering 2024Quote: ... Huh-7 cells were cultivated in DMEM (ThermoFisher Scientific) supplemented with 10% fetal calf serum (FCS ...
-
bioRxiv - Molecular Biology 2024Quote: ... 7 μL of RNase I (Invitrogen, Cat. No. AM2249) was added to the clarified lysates and digestion was carried out for 1 hour at 4 °C ...
-
bioRxiv - Neuroscience 2020Quote: ... Fluo-4 (Fluo 4 AM/Fluo 4 direct, Life Technologies) was used according to the manufacturer’s instructions and a previously published protocol (54) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were analyzed with the MACSQuant cell analyzer (3 lasers, 10 parameters, Miltenyi Biotech) or the AttuneNxT acoustic focusing cytometer (4 lasers, 14 parameters, Thermo-Fisher Scientific). Data were processed using FlowJo software (ver.9.9 or 10.7 ...
-
bioRxiv - Immunology 2021Quote: ... was added to a concentration of 1X and 12.5-30 μg of total protein from each sample was resolved on NuPAGETM 4-12% BisTris (Phospho-PMK-1 and Total-PMK-1) or NuPAGETM 3-8% TrisAcetate (TIR-1::3xFLAG) gels (ThermoFisher Scientific), transferred to nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were washed 2-3 times (200 rpm, 5 min at 4°C) in eBioscience™ Permeabilization buffer (250 µl/well) (Invitrogen) and resuspended in eBioscience™ Fixation/Permeabilization solution (Invitrogen ...
-
bioRxiv - Neuroscience 2021Quote: ... Membranes were then washed 3 times for 4 min with TBST and treated with Pierce ECL Western Blotting Substrate (Thermo Scientific) according to manufacturer protocol ...
-
bioRxiv - Microbiology 2020Quote: ... The purified linearized vector and digested foxA gene were ligated (1:3 molar ratio) overnight at 4°C using T4 DNA ligase (Thermo Scientific). The construct was transformed into competent E ...
-
bioRxiv - Cancer Biology 2019Quote: ... LDHA proteins in tumor extract were separated on 4% to 20% Tris-Glycine gel and CD31 was separated on NuPAGE 3 to 8 % Tris-Acetate gel (Life Technologies) by SDS-PAGE and were transferred to nitrocellulose membrane ...
-
bioRxiv - Genomics 2020Quote: ... where 2–3 mm of the terminal ends of 3–4 filaments from the left side each fish were collected and placed in RNAlater (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... Slices were then rinsed 4 x 15 min in PBS using the nuclear counterstain TO-PRO-3 Iodide (1:2000, Thermo Fisher). For preservation of immunofluorescence ...
-
bioRxiv - Bioengineering 2021Quote: ... an Agilent LC-MS system consisting of a 1100 HPLC and a 1946D single quadrupole ESI mass spectrometer equipped with a MabPac RP column (3 × 50 mm, 4 µm, Thermo Scientific) or 2 ...
-
bioRxiv - Bioengineering 2020Quote: ... We performed ERBB2 SNAPD on a 9:1 mixture of MOLT-4 and SK-BR-3 cells stained with CellTrace™ Calcein Red-Orange AM (Invitrogen). We then reinjected these droplets onto a dielectric sorting device (Fig ...
-
Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: HeLa cell pellets (∼2×107) or MCF7 cell pellets (∼4×107) were lysed with 3 mL Mammalian Protein Extraction Reagent (Thermo Scientific), supplemented with 2X HALT protease inhibitor (Thermo Scientific) ...
-
bioRxiv - Neuroscience 2020Quote: ... for 1 h at RT and then incubated for 3 days at 4°C with the monoclonal mouse anti-NeuN antibody conjugated with Alexa-488 (clone A60, Life Technologies) at a concentration of 0.5 µg mL-1 into blocking buffer ...
-
bioRxiv - Microbiology 2020Quote: ... and then rinsed 3 times in sterile water and 4 times in Dulbecco’s Phosphate-Buffered Salines (DPBS) (ThermoFisher Scientific, Waltham, MA) [4] ...
-
bioRxiv - Biophysics 2021Quote: ... plus 1% penicillin/streptomycin in a humidified incubator at 37 °C and 5% CO2 and passaged every 3-4 days using 0.05% of Trypsin-EDTA (Thermo Fisher Scientific) to lift the cells from the culture dish ...
-
bioRxiv - Cancer Biology 2020Quote: ... The medium was changed every 2-3 days and organoids were passaged every 2-4 weeks by dissociation with 1 ml of TrypLE Express (Life Technologies) at 37°C for 5 min ...
-
bioRxiv - Cell Biology 2021Quote: ... were transiently transfected into 8 × 104 U2OS 2-6-3 cells in 4-well chamber slides using Lipofectamine 2000 reagent (Invitrogen, 11668019) according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2020Quote: ... were loaded on to NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate 1.0 mm Mini Protein Gels (Invitrogen, NP0321, EA0375PK2) buffer alongside PageRuler Protein Ladder (5 µL/lane ...
-
bioRxiv - Neuroscience 2022Quote: ... CAT-1 (encoded by SLC7A1) and CAT-3 (encoded by SLC7A3) had 4 substrates including L-Lysine hydrochloride (Fisher Scientific #BP386), L-Histidine (Sigma-Aldrich #H6034) ...
-
bioRxiv - Molecular Biology 2022Quote: ... 4 µg of an HA-tag antibody (MBL, M132-3) was mixed with 20 µL of Dynabeads Protein G (Invitrogen, #10004D) and subsequently added to extracts ...
-
bioRxiv - Neuroscience 2022Quote: ... Samples were subjected through high pH reverse phase fractionation into 3 or 4 fractions and all fractions were run independently on an Orbitrap Fusion (Thermo Scientific) mass spectrometer coupled to an Acquity M-Class nanoLC (Waters Corporation) ...
-
bioRxiv - Immunology 2022Quote: ... Proteins and DNAs were incubated for 30 min at 4 °C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 °C ...
-
bioRxiv - Neuroscience 2022Quote: Cultured neurons at 19-21 DIV were incubated with 3 μM of the calcium dye Fluo-4 AM (Thermo Scientific, Sweden) in DMSO(Sigma-Aldrich ...
-
bioRxiv - Microbiology 2020Quote: ... followed by incubation for 3 hr at 4°C with 250 μg of GST-fusion protein coupled to glutathione-agarose (Thermo Scientific). Beads were then washed four times (pull down buffer containing 0.1% Triton X-100 ...
-
bioRxiv - Immunology 2020Quote: ... RAG was reconstituted in RAG1-deficient v-Abl cells via retroviral infection of cells with the pMSCV-RAG1-IRES-Bsr and pMSCV-FLAG-RAG2-GFP vectors followed by 3-4 days of Blasticidin (ThermoFisher, #R21001) selection to enrich for cells with virus integration.
-
bioRxiv - Cell Biology 2021Quote: ... were isolated from 3-4 days old euthanized rats and were plated in α-MEM medium supplemented with 10% fetal bovine serum (Gibco) and 1% Penicillin-Streptomycin solution (Hyclone ...
-
bioRxiv - Bioengineering 2020Quote: ... The reaction was monitored using an Agilent G1946D LC/ESI-MS system equipped with a MabPac RP column (3 × 50 mm, 4 µm, Thermo Scientific). Elution conditions were as follows ...
-
bioRxiv - Bioengineering 2020Quote: ... All samples were analyzed using an Agilent 1100 HPLC system equipped with a MabPac RP column (3 × 50 mm, 4 µm, Thermo Scientific). Elution conditions were as follows ...
-
bioRxiv - Molecular Biology 2019Quote: ... incubated at 95°C for 5 min and the supernatant loaded onto a either 3-8% Tris-acetate or 4-12% Bis-Tris gradient gels (Life Technologies).
-
bioRxiv - Molecular Biology 2020Quote: ... adult cardiomyocytes isolated from 3-4 month old mice were washed in PBS containing protease and phosphatase inhibitor cocktail (PIC; Thermo Scientific) and then fixed in 1% formaldehyde at 25°C for 8 min for cross-linking and followed by quenching by 0.125 M glycine at 25°C for 8 min as previously described ...