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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for 6 CHLORO 4 METHYL 3 PHENYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... the sections were incubated with 4’,6-diamidino-2-phenylindole (DAPI; D1306, Thermo Fisher Scientific; 1:1000 in PBS) for 2min at room temperature to counterstain the nucleus before being washed twice in PBS ...
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bioRxiv - Plant Biology 2024Quote: DAPI (4′,6-diamidino-2-phenylindole) solution: Dilute 1 mg/ml DAPI (ThermoFisher, Cat. No. 62248, Rockford, IL, USA) to a final concentration of 0.5 µg/ml in 1x PBST (0.1% Tween-20).
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bioRxiv - Cell Biology 2024Quote: ... 1:2000 LipidTOX was added along with 0.25 μg/ml of 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes) for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were washed in PBS and nuclei were stained with 4’-6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) and mounted with an aqueous mounting medium (Aqua-Poly/Mount ...
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bioRxiv - Cell Biology 2024Quote: ... Gels were functionalized with 0.1M sulfo-succinimidyl 6-(4’-azido-2’-nitrophenylamino) hexanoate (Sulfo-SANPAH) (Cat# 22589, Thermo Scientific) dissolved in HEPES before overnight incubation in collagen type-I solution (Catalogue # A1048301 ...
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bioRxiv - Neuroscience 2020Quote: ... were quantified in autaptic neuronal cultures using N-(3-triethylammoniumpropyl)-4-(4-(dibutyl amino) styryl) pyridinium dibromide (FM1-43, Thermo Fisher Scientific, Waltham, MA, USA), similarly as in our previous report (Kawano et al. ...
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bioRxiv - Neuroscience 2023Quote: ... were visualized using N-(3-triethylammoniumpropyl)-4-(4-(dibutyl amino) styryl) pyridinium dibromide (FM1-43FX, a fixable analog of FM1-43 membrane stain, Thermo Fisher Scientific, Waltham, MA, USA). To stain the presynaptically active synapses of autaptic cultured neurons ...
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bioRxiv - Molecular Biology 2021Quote: ... Primers were designed using Methyl Primer Express®Software v1.0 (Applied Biosystems/ThermoFisher Scientific) to amplify specific regions of the genome following bisulphite conversion ...
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bioRxiv - Molecular Biology 2021Quote: ... Primers were designed using Methyl Primer Express®Software v1.0 (Applied Biosystems/ThermoFisher Scientific) to amplify specific regions of the genome following bisulphite conversion ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: Membrane potential was evaluated using the image-iT TMRM (tetramethylrhodamine, methyl ester) reagent (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... They were then incubated with Tetramethylrhodamine-methyl-ester (TMRM) (Thermo Fisher Scientific, United States) for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were cleared in methyl salicylate and mounted using DPX mountant (Fisher Scientific, #18404). Images of whole mounts were captured at different magnifications using stereoscope (Magnus MSZ-TR).
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bioRxiv - Microbiology 2022Quote: Membrane potential was evaluated using the image-iT TMRM (tetramethylrhodamine, methyl ester) reagent (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... or TMRM (Tetramethylrhodamine, methyl ester; 10 nM, 37°C) (Molecular Probes, Invitrogen, Paisley, UK). Following treatment ...
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bioRxiv - Physiology 2020Quote: ... or TMRM (Tetramethylrhodamine, methyl ester; 10 nM, 37°C) (Molecular Probes, Invitrogen, Paisley, UK). Following treatment ...
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bioRxiv - Neuroscience 2022Quote: ... gels were incubated with 10 µM BODIPY TR Methyl Ester (Thermofisher, catalog no. C34556) in 1× PBS for 1 h on a rocking platform at RT and washed three times with 1× PBS for 30 min each ...
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bioRxiv - Neuroscience 2022Quote: ... gels were incubated with 10 µM BODIPY TR Methyl Ester (Thermofisher, catalog no. C34556) in 1× PBS for 1 h on a rocking platform at RT and washed three times with 1× PBS for 30 min each ...
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bioRxiv - Physiology 2022Quote: ... 60 μL of N-methyl-N-trimethylsilyltrifluoracetamide (MSTFA with 1%TMCS, ThermoFisher Scientific #TS48913) was added automatically via the auto sampler and incubated for 30 minutes at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The crude peptide was precipitated in tert-butyl methyl ether (TBME, ACROS Organics, AC378720025). After the supernatant was removed by centrifugation ...
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bioRxiv - Biochemistry 2023Quote: ... N-methyl-N-(trimethylsilyl)-triAuoroacetamide (TMS) and ammonium acetate were purchased from Thermo Fisher Scientific (Waltham) ...
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bioRxiv - Cell Biology 2023Quote: ... siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’, 5’-GGGAUGAUGAGACCAUGUAtt-3’, 5’- AAAUCCUAAUGGAGACAAAtt-3’, Ambion)
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bioRxiv - Immunology 2021Quote: ... Samples from Biosafety Level 3 were inactivated for 2 hours at RT with 4% paraformaldehyde (ThermoFisher Scientific) after extracellular and intracellular staining.
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bioRxiv - Cell Biology 2019Quote: ... For differentiation cells were switched to M254 medium for 3-4 population doublings (ThermoFisher Scientific, Life Technologies).
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bioRxiv - Cell Biology 2019Quote: ... For differentiation cells were switched to M254 medium for 3-4 population doublings (ThermoFisher Scientific, Life Technologies).
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bioRxiv - Neuroscience 2020Quote: ... on a 4-12% bis-tris gel (Genescript) in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). The gel was then fixed for 30 min in 10% acetic acid/50% methanol ...
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bioRxiv - Cell Biology 2021Quote: ... For live imaging of cells as described for fatty acids up-take we treated cells at day 5 of differentiation with BODIPY™ (10μM) C12 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Invitrogen). In addition ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged after reaching 70-85% confluency (every 3-4 days) using Accutase (Gibco #A11105-01) to disperse into single cells and replated in mTeSR1 supplemented with 1% P/S containing 10 µM Rock Inhibitor (Y-27632 ...
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bioRxiv - Cell Biology 2021Quote: ... as previously described.13 Cells were passaged every 3-4 days using Collagenase Type IV (Life Technologies). Expression of pluripotency markers was analyzed by flow cytometry using SOX2 (Cell Signaling ...
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bioRxiv - Molecular Biology 2019Quote: ... Immunoprecipitation was performed at 4°C for 3 hr with 50 µl protein G dynabeads (Thermo Fisher). Beads were washed five times with ice-cold Wash buffer 1 (50 mM Hepes-KOH (pH7.6) ...
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bioRxiv - Systems Biology 2019Quote: ... 3-30μg protein per well was separated using NuPAGE precast 4-12% polyacrylamide gel system (ThermoFisher Scientific). Immunoblotting was carried out using an X-Cell II blot module (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... 3 hours for E14.5 and 4 hours for E18.5 tissues and then blocked in CAS Block (ThermoFisher) for at least two hours ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were subcultured every 3-4 days by washing with Dulbecco’s phosphate buffered saline (DPBS, GibcoTM, ThermoFisher) and detached with trypsin (GibcoTM ...
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bioRxiv - Immunology 2022Quote: ... Epithelium was then collected by centrifugation (3 min, 300g, 4 ºC) and digested by TrypLE express (Gibco) for 1 minute at 37ºC and vortexed ...
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bioRxiv - Microbiology 2022Quote: ... adding 3 mL HPLC grade methanol and 2 mL HPLC grade chloroform (C297-4, Thermo Fisher Scientific), then shaking at 22°C for 1 hr ...
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bioRxiv - Microbiology 2023Quote: ... N-(3-triethylammoniumpropyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64) (Thermo Fisher Scientific, Waltham, MA, USA) as previously described (9) ...
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bioRxiv - Cell Biology 2023Quote: ... hESCs were passaged at 70-80% confluency every 3-4 days into Geltrex coated dishes (Life Technologies) using gentle cell dissociation reagent (STEMCELL-Technologies) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD, catalog number: D7757) was purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated by NuPAGE 3-8% or 4-12 % Tris-Acetate Midi Gel (Invitrogen, Cat# WG1402BX10) and transferred to nitrocellulose membranes (Fisher ...
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bioRxiv - Immunology 2023Quote: ... for 3-4 days to OD 0.6 in tryptone soya broth (Oxoid) supplemented with 10% FCS (Gibco) and the antibiotics above ...
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bioRxiv - Synthetic Biology 2024Quote: ... Samples were stained 3 days at 4°C with phalloidin (0.66 µM, Alexa Fluor 488, Thermofisher, #A12379) and DAPI (0.3 mg/ml ...
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bioRxiv - Genomics 2023Quote: ... and CRISPRmap library plasmid (2:3:4 ratio by mass) using Lipofectamine 3000 (Thermo Fisher Scientific L3000001) in lentiviral packaging medium (Opti-MEM (Thermo Fisher Scientific 31-985-070 ...
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bioRxiv - Cancer Biology 2021Quote: ... Culture medium was refreshed every 2–3 days and organoids were passaged 1:2–1:6 every 7–21 days using TrypLE Express (Thermo Fisher). For co-culturing ...
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bioRxiv - Bioengineering 2022Quote: Primary neonatal cardiomyocytes were isolated from C57BL/6 mouse neonates on postnatal days 3-5 using the Pierce Primary Cardiomyocyte Isolation Kit (Thermo Fisher) as previously described (14) ...
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bioRxiv - Developmental Biology 2019Quote: ... blastocysts were collected from uterine horns and put on culture for 3-6 days in ES derivation medium composed of GlutaMAX/DMEM (Gibco, 31966), 15% FBS (Biowest) ...
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bioRxiv - Systems Biology 2019Quote: A mate-pair library with 3-6 kb fragments was prepared from pure genomic DNA using Ion TrueMate Library Reagents (Life Technologies). The Ion PGM™ template OT2 400 kit (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Embedded tissues were cut into 3-6 µm sections using a paraffin microtome (Leitz) and placed on SuperFrost® Plus slides (Menzel Gläser, Thermo Scientific). Deparaffination and antigen retrieval was done as described previously (Link et al. ...
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bioRxiv - Neuroscience 2020Quote: ... All 27 samples from 3 groups and 6 GIS were divided and labeled using two sets of 5 mg 11 plex TMT reagents (Thermo Scientific A34808 ...
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bioRxiv - Immunology 2022Quote: ... and the wells were washed five times with wash buffer before the addition of 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS, #37615, Thermo Fisher Scientific). Optical density was read 40 min later at 405 nm using a plate reader (SpectraMax i3 ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293t were replated every 3 days at a split ratio of 1 in 6 following dissociation with Trypsin 0.05% EDTA (Gibco, Life technologies).
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bioRxiv - Neuroscience 2021Quote: ... and immersed in reagent-2 (diluted 1:2 in PBS) for 6-24 h before incubated in reagent-2 containing TO-PRO-3 (1:5,000, Thermo Fisher Scientific) for additional 7-10 days ...