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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for 6 CHLORO 4 METHYL 3 PHENYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Mitochondrial membrane potential was evaluated with a flow cytometer and Tetramethylrhodamine Methyl Ester (TMRM, Invitrogen™) stain ...
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bioRxiv - Synthetic Biology 2020Quote: Quantification of methyl ketones was performed on a Trace GC Ultra (Thermo Scientific, Waltham, MA, USA) equipped with a flame ionization detector (FID) ...
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bioRxiv - Neuroscience 2023Quote: ... Tetramethylrhodamine methyl ester (TMRM, #2321830), horseradish peroxidase (HRP, #9003990) and AmplexUltra-Red (#2365697) were from Invitrogen Thermo Fisher Scientific (OR ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated with 3X cholesterol or 5mM methyl beta cyclodextrin (MßC) (#377110050, Thermo Fisher) (doses chosen for biological effectiveness but minimal cellular toxicity [Supplementary Figures 2 and 3] ...
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bioRxiv - Genetics 2024Quote: ... Oligonucleotides for gene promoters were designed in Methyl Primer Express™ Software v1.0 (Applied Biosystems, USA), following directions in Wojdacz et al ...
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bioRxiv - Cell Biology 2020Quote: ... BN-PAGE was performed using NativePAGE 3-12% or 4-16% Bis-Tris gels and associated running buffer (Invitrogen). The cathode buffer was supplemented with 0.002% (w/v ...
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bioRxiv - Molecular Biology 2019Quote: ... and (3) 110,000 × g for 2 hours at 4°C with a Sorvall Discovery SE ultracentrifuge (Thermo Fisher Scientific) with an AH-650 rotor (k factor ...
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bioRxiv - Immunology 2021Quote: ... Excess liquid was blotted for 3 seconds with a force of -4 using a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
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bioRxiv - Neuroscience 2020Quote: ... third instar larvae were dissected and stimulated with a HL-3 solution of 90 mM K+/0.5 mM Ca2+/4 μM fixable FM1-43 (Invitrogen) for 1 min ...
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bioRxiv - Genomics 2020Quote: ... Two TaqMan probes targeting the MLLT3 exon 3-4 and exon 9-10 splice junctions (ThermoFisher, Hs00971092_m1 and Hs00971099_m1) were used with cDNA diluted 1:5 in dH2O to perform qPCR for six replicates per sample on an Applied Biosystems Quant Studio 6 Flex ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were loaded with culture medium containing 3 μM of the fluorescent calcium indicator Fluo-4/AM (Life Technologies) and Pluronic F-127 (0.2% w/v ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody incubation was performed for 3-4 days and DiD (Thermo Fisher Scientific-Molecular Probes L7781; 1µg/ml) labeling was added from the second day to show the whole brain morphology ...
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bioRxiv - Synthetic Biology 2021Quote: N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (NBD-PE) was supplied by Thermo Fisher Scientific Inc ...
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bioRxiv - Microbiology 2021Quote: ... Cell monolayers were then stained with 3 mL of overlay containing a 1:1 mixture of 1.2% oxoid agar with 4% neutral red (Gibco) and 2X DMEM with 2% (vol/vol ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were subcultured every 3-4 days as follows: cells were washed with phosphate buffered saline (PBS, Life Technologies) and then incubated with trypsin (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... incubated for 10 min in the dark at room temperature with FM 4-64FX (3 μg ml-1) (ThermoFisher). Cells were applied to a 5% agarose pad containing M9 minimal media with glucose (0.4%) ...
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bioRxiv - Cancer Biology 2022Quote: ... RT reactions were performed with 3-4 µg total RNA using oligo-dT and SuperScript III RT (ThermoFisher Scientific). QPCR was performed as previously described [20] ...
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bioRxiv - Genetics 2022Quote: Immunoprecipitations were performed using 1 μg of Anti-Trimethyl-Histone-3-Lys-4 (-Thermo Fisher Scientific; catalog# PA5-17420) overnight at 4 ◦ C ...
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bioRxiv - Cell Biology 2019Quote: ... Liposomes containing PtdIns(4)P- or PtdIns(3)P were independently labeled with DiI and DiD (Thermo Fisher Scientific) lipid dyes and detected by excitation at 561 nm and 640 nm ...
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bioRxiv - Molecular Biology 2020Quote: ... heated at 95°C for 3 min and seprated on a Novex WedgeWell 4-12% Tris-Glycine Gel (Invitrogen). Proteins were transferred to an PVDF Transfer Membrane (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... After 3 days the overlay was removed and cell monolayers were fixed with 4% formaldehyde (28906, Thermo Fisher Scientific) at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Uptake of fatty acids was quantified after incubation with 1uM 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic acid (Bodipy-FL C16, Thermo Fisher) at 37 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Media was changed every 3 to 4 days and cells were passaged at nearly 90% confluence using 0.25% trypsin (Gibco). B16F0 cells were verified for mycoplasma contamination at Charles River Laboratories for cell line testing ...
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bioRxiv - Cell Biology 2021Quote: ... or 5 µM BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine) (Invitrogen, D3521) together with 5 µM delipidated BSA (as a delivery carrier ...
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bioRxiv - Cell Biology 2021Quote: ... WT and SIRT1 KO mESCs cultured in serum-free ESGRO medium or serum-containing M10 medium were incubated with 5 µM BODIPY™ FL C5-Sphingomyelin (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosyl Phosphocholine) (Invitrogen, D3522) or 5 µM BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine ...
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bioRxiv - Bioengineering 2022Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (16:0 NBD) were purchased from Invitrogen. Phosphate-buffered saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... 4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (BODIPY-C12, Invitrogen D3835), was dissolved in 100% ethanol and conjugated to 10% bovine serum albumin ...
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bioRxiv - Plant Biology 2022Quote: ... pH 5.5 and 100 μM 3′,5′-dimethoxy-4′-hydroxyacetophenone (acetosyringone) (115540050; Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (D8418 ...
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bioRxiv - Neuroscience 2022Quote: 100 to 150 3-dpf zebrafish larvae were bathed in Yo-Pro-1 (4 µM, 30 min) (Invitrogen, Y3603), then washed 3 times in blue water to label lateral line hair cells ...
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bioRxiv - Neuroscience 2022Quote: ... The craniotomy was then sealed with two glass coverslips (3 mm and 4 mm diameter, Thermo Fisher Scientific, UK) glued to one another using optical adhesive (Norland ...
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bioRxiv - Physiology 2022Quote: ... Cells were fixed with ice-cold 4% paraformaldehyde solution and cell nuclei were stained with To-Pro-3 (Invitrogen). Samples were mounted with Mowiol (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... BODIPY-CE (cholesteryl 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3- dodecanoate) was obtained from Life Technologies. Lipids (with the exception of NBD-LPC ...
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bioRxiv - Biochemistry 2022Quote: ... Acly-/- MEFs were maintained at <80% confluence (passaged every 3-4 days) in high glucose DMEM (Gibco #11965-084) with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... cells at passage 3-4 were seeded in to black walled clear bottomed 96 well plates (Pierce, ThermoFisher Scientific) at a density of 7,500 cells per well ...
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bioRxiv - Cancer Biology 2023Quote: ... reagent at a 4:2:3 ratio of sgRNA/overexpression-construct: pVSVG: psPAX2 in Opti-MEM media (Life Technologies, Thermo Fisher Scientific Inc ...
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bioRxiv - Immunology 2023Quote: ... and packaging vector psPAX2 were mixed in a 4:3:1 ratio in OPTI-MEM (Thermo Fisher Scientific, 31985070) and PEI (Polysciences ...
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bioRxiv - Biochemistry 2023Quote: ... SDS-PAGE was carried out on either NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate gels (Invitrogen). Western blotting analysis was carried out according to standard protocols with the antibodies listed in Table S5.
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bioRxiv - Molecular Biology 2023Quote: ... Cells were passaged every 3-4 days during the log phase of growth with Trypsin/EDTA solution (Gibco, R001100) following a PBS wash ...
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bioRxiv - Genomics 2023Quote: ... 3-4 days post-nucleofection (depending on cell confluency) selection was started using 400 ng/mL Puromycin (ThermoFisher A1113802) in FM (PFM) ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 30 min at 4 °C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: BODIPY FL C16 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic Acid, Invitrogen D3821) was used to assess the ability of 3T3s to transport fatty acids into the cell from their surroundings ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed with PBS 3 times then fixed with 4% paraformaldehyde (PFA) in 1x PBS (Thermofisher, cat#043368.9M) at room temperature for 15 min in the dark ...
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bioRxiv - Developmental Biology 2022Quote: ... DAB-developed samples were stored in 70% glycerol and counterstained with 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific, #D3571, 1:1000) nuclear marker ...
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bioRxiv - Immunology 2022Quote: ... containing 4′,6-diamidino- 2-phenylindole (DAPI) and were transferred onto microscope slides with large-orifice 200 µL-tips (Fisher Scientific, 02707134). The following antibodies or reagents were used ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were then washed as above and incubated in 1 μg/ml of 4′,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) at RT for 10 min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DAPI (4-6-diamidino-2-phenylindole) and DyLight 488-or DyLight 555-labeled secondary antibodies (1:400, Thermo Fisher Scientific, Waltham, MA) were added for 1.5h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected with 4-6 μg linearized targeting vector DNA (ROSA26-SpCas9) using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, USA), selected with 1400-1600 μg/mL G418 (Genaxxon Bioscience ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated from liver tissues of SHR and WKY (4-6 weeks old) using the TRIzol reagent (Invitrogen, Waltham, USA) followed by purification using Nucleospin columns (Machery-Nagel ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were subsequently rinsed in PBS and counterstained with 1:5000 dilution of DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride, Invitrogen, Eugene, OR) in deionized water for 3-5 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) as counterstaining.