Labshake search
Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for 14 3 3 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... The 16S rRNA gene was amplified with the primers 8f (5′-AGAGTTTGATCCTGGCTCAG-3′; Weisburg et al. 1991) and 1520 r (5′-AAGGAGGTGATCCAGCCGCA-3′; Edwards et al. 1989) (Invitrogen, CA, USA). A volume of 0.5 μL DNA extract was used for 50 μL PCR reactions containing 2 units Taq DNA polymerase ...
-
bioRxiv - Molecular Biology 2023Quote: ... The following day the miR-423-5p or miR-Ctrl vector was cotransfected with either the Cacna2d2-3’UTR or the Cacna2d2 mut 3’UTR reporter vectors using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... and 40 cycles of PCR (95°C for 3 seconds, 60°C for 30 seconds) on a QuantStudio 3 (Applied Biosystems, MA).
-
bioRxiv - Biochemistry 2023Quote: ... samples were blotted at 100% humidity for 3 s (13°C, 0 s drain time, blot force -3 to +2) with a Vitrobot Mark IV (Thermo Fisher Scientific) and plunged into liquid ethane ...
-
bioRxiv - Cell Biology 2023Quote: Supernatants of cell culture medium collected during medium exchanges were analysed for albumin (10242, Diagnostic Systems) and 3-beta-hydroxybutyrate (Autokit 3-HB, Fujifilm Wako) on an Indiko Plus chemical analyzer (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from 6 pairs of P21 mouse testes (3 pairs of wild type and 3 pairs of Mov10-/-) using TRIzol reagents (Thermo Fisher Scientific). 1 μg of total RNA from each sample was used to generate RNA-seq libraries using TruSeq Stranded mRNA Library Preparation Kit Set A (Cat ...
-
bioRxiv - Biochemistry 2023Quote: The ibpA 5’ UTR-gfp mRNA produced from CUGA®7 in vitro transcription kit (Nippon Gene) was attached to a 3’-terminal biotinylated nucleotide using Pierce™ RNA 3’ End Biotinylation kit (Thermo Scientific). The biotin-labeled mRNA (0.1 µM ...
-
bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
-
bioRxiv - Cell Biology 2023Quote: ... Treatments were incubated 2 hours before addition of the MTS (3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) reagent (Thermo-Fisher Scientific, Waltham, MA) and incubation was continued another 1.5 hours ...
-
bioRxiv - Physiology 2023Quote: ... Digitonin solubilized mitochondria were loaded 3-12% Bis-Tris Invitrogen™ Novex™ NativePAGE™ 3-12% acrylamide gel (1mm) (Invitrogen, BN2011BX10) using the anode running buffer (Invitrogen ...
-
bioRxiv - Biochemistry 2023Quote: ... Four microliters of LotA7-544 was loaded onto a glow-discharged grid and blotted for 3 s with blot force 3 using Vitrobot Mark IV (Thermo Fisher, U.S.A.).
-
bioRxiv - Neuroscience 2023Quote: ... Neuron-cancer cocultures were quantified for neuronal outgrowth by identical image generation and processing with additional pre-incubation (3 drops/mL for 90 min at 37◦C) of NucRed Dead 647 ReadyProbes™ Reagent (TO-PRO-3 iodide) (Invitrogen, #R37113) for dead cells and NucBlue Live ReadyProbes™ Reagent (Hoechst 33342 ...
-
bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
-
bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
-
bioRxiv - Cell Biology 2024Quote: ... For SNX5 and SNX6 the following siRNA sequences were used: SNX5 (5′-UUAGUUUCAGCCCGAAGCAUC-3’), SNX6 (5′-UUAUGAGGUAGACGACUAAAU-3’) (Wassmer et al., 2007), VPS35 (ID 132357, AM16708) (Predesigned – Thermo Fisher Scientific). Nontargeting control siRNA (control ...
-
bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was lawned with goat anti-human IgG Fc (50 µg/mL ...
-
bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was coupled directly to mAbs diluted to 10 µg/mL in 10 mM sodium acetate (pH 4.5 ...
-
bioRxiv - Physiology 2024Quote: ... 2 µl cDNA was amplified with TaqMan™ qPCR (38 cycles, 3 technical replicates of 3 biological replicates) using master mix (Thermo Fisher) and the following TaqMan assays ...
-
bioRxiv - Biophysics 2024Quote: ... and N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (BODIPY FL DHPE) were purchased as solids from Invitrogen (Thermo Fischer Scientific), Avanti Polar Lipids ...
-
bioRxiv - Cancer Biology 2024Quote: ... 10 μl of sample was saved for 3′ -biotin labeling for visualization using Pierce RNA 3′ End Biotinylation Kit (Life Technologies 20160) according to manufacturer’s protocol ...
-
bioRxiv - Immunology 2024Quote: ... and SB28 RRV-IRF8 (100% transduced) cells were plated at 1x105 cells (n=3 per time point) and counted (Thermofisher Countess 3)at 24 and 48 hours post-seeding ...
-
bioRxiv - Microbiology 2024Quote: ... 1 μg of plasmid and 3 μL of Lipofectamine 2000 (m/v = 1:3) were diluted in 0.1 mL of Opti-MEM (Gibco, 31985070, Gaithersburg, MD). After 5 min of incubation ...
-
bioRxiv - Microbiology 2024Quote: ... was added at a 1:2000 dilutions for 1 h at 37 °C, followed by adding TMB (3, 3, 5, 5′-tetramethylbenzidine) peroxidase substrate (Thermo Fisher Scientific) for 30 min ...
-
bioRxiv - Microbiology 2024Quote: ... and a pan VSG reverse primer which binds to a conserved 14bp region of the 3’ UTR (5’-GATTTAGGTGACACTATAGTGTTAAAATATATC-3’) with AmpliTaq Gold (Applied Biosystems, 4398881) (anneal & extension 60C 1m ...
-
bioRxiv - Physiology 2020Quote: ... Slides were rinsed in PBS 3×5min and then incubated for 1h at room temperature in goat anti-rabbit AF647 (1/250, 21245, Life Technologies) or donkey anti-goat AF647 (1/500 ...
-
bioRxiv - Immunology 2021Quote: ... Samples were washed 3 x 10 min with PBS/PBS-T and incubated with secondary antibody anti-Rabbit-568 (1:500, Thermofisher #A21069), anti-Rabbit-488 (1:100 ...
-
bioRxiv - Neuroscience 2020Quote: ... tissues were washed in PBS (3×5 min) and incubated with secondary antibodies conjugated to Alexa Fluor 488 (1:400; goat anti–rabbit; Life Technologies) or Alexa Fluor 594 (1:400 ...
-
bioRxiv - Neuroscience 2020Quote: ... Slices were then washed with 0.1% TritonX-100 / PBS for 1.5 hours 3 times and incubated with Alexa Fluor 555-conjugated donkey anti-rabbit IgG (1:250, ThermoFisher, #A-31572) and/or Alexa Fluor 555-conjugated donkey anti-mouse IgG (1:250 ...
-
bioRxiv - Neuroscience 2020Quote: ... The sections were then incubated with the following secondary antibodies for 3 h at room temperature: anti-rabbit IgG-Alexa488 and anti-rat IgG-Alexa488 (1:1000 for both; Invitrogen Co). Sections were mounted on microscope slides and embedded with Vectashield that includes DAPI for nuclear staining (Vector Laboratories ...
-
bioRxiv - Cell Biology 2022Quote: ... Blots were washed 3×10 minutes in PBS-T and incubated with secondary antibody (goat anti-rabbit IgG HRP conjugate, Invitrogen, G21234) diluted in 1% milk +BSA (1:5000 ...
-
bioRxiv - Cell Biology 2022Quote: ... Blots were washed 3 times in TBS-T and incubated with secondary antibody (Goat anti-rabbit IgG HRP conjugate, Invitrogen, G21234) for 1 hour at room temp ...
-
Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic ActuatorsbioRxiv - Biochemistry 2019Quote: ... Cells were then washed with PBS (3 × 5 min) before incubation with anti-rabbit AlexaFluor 647 secondary antibody (Life Technologies A21245) at 1:500 dilution in antibody dilution buffer ...
-
bioRxiv - Cancer Biology 2019Quote: ... slides were washed 3 times in TBS and incubated with the appropriate secondary antibodies: goat-anti-rabbit-Alexa594 (Invitrogen; 1:1000) plus goat-anti-mouse-Alexa488 (Invitrogen ...
-
bioRxiv - Biochemistry 2020Quote: ... Primary antibodies were removed by 3 washes with 2% BSA 1x PBS and incubated with 2 μg/ml anti-rabbit IgG Alexa Fluor 594 (Thermo Fisher) for 2 h in the dark at 4°C ...
-
bioRxiv - Neuroscience 2019Quote: ... Sections were washed in 0.3% Triton-X/PBS 3× 5 min and incubated with secondary antibody (goat anti-rabbit-Alexa Fluor 594: 1:800, Life Technologies) for one hour ...
-
bioRxiv - Neuroscience 2023Quote: ... Slides were then washed with 1X PBS for 3 10-minute washes before applying the secondary antibody goat anti-rabbit Alexa 647 (Invitrogen A21245), which was allowed to incubate at RT for 2 hours ...
-
bioRxiv - Neuroscience 2023Quote: ... samples were protected from light going forward and incubated for 5 days at 37 °C in 1.6 ml of PTwH + 3% donkey serum containing a 1:250 dilution of secondary antibody (donkey anti-Rabbit Alexa 647, ThermoFisher Scientific). Samples were washed 5x 5 min and incubated overnight at rt in 2 mL of PTwH.
-
bioRxiv - Molecular Biology 2022Quote: ... and slides were washed 3 x 5min in PBS before the application of 1:500 goat α-rabbit AlexaFluor488 antibody (ThermoFisher) for 30min ...
-
bioRxiv - Neuroscience 2022Quote: ... slices were washed 2 × 5 minutes in 0.1 M PBS and incubated for 3 hours in a solution containing donkey anti-rabbit conjugated to Alexa Fluor 647 (1/500 dilution; Invitrogen, UK). After secondary antibody incubation ...
-
bioRxiv - Immunology 2023Quote: ... whole-cleared tissues were washed for 3-4h-hours and incubated with a secondary Alexa Fluor™ 647 donkey anti-rabbit antibody (A-31573, Invitrogen) for another 3 days in room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times with PBS and AlexaFluor (AF488, AF568) conjugated anti-rabbit or anti-mouse antibodies (#A-11008, A-11004 Fisher Scientific) were added and incubated for 1h at room temperature (RT) ...
-
bioRxiv - Cancer Biology 2023Quote: ... The cells were then washed with cold 1X PBS three times for 3 min each and incubated with Alexa 555-labeled anti-rabbit and Alexa 488-labeled anti-mouse secondary antibody (Thermo Fisher) at room temperature for 1 hour ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... + hydrogen peroxidase 3% (1:100, Alexa Fluor 488 Tyramide SuperBoost Kit, goat anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific, Ghent, Belgium) + Tris Buffer HCl pH 7,4 (1:1 ...
-
bioRxiv - Cell Biology 2023Quote: ... 24 hours after induction of GFP-KIF18A and endogenous KIF18A knockdown cells were fixed for 3 minutes in - 20oC methanol and subsequently stained for rabbit GFP 1:500 (Invitrogen, A11122) for one hour at room temperature with shaking.
-
bioRxiv - Microbiology 2023Quote: ... Cells were washed three times and incubated with Alexa546-anti- rabbit secondary antibody (1:1000 in 3% BSA/PBS, 200 µl/well, Invitrogen, A11035) for another hour at 37 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... and then incubated with secondary antibody (AlexaFluor 546 donkey anti-mouse for PV, goat anti-rabbit for GluR2/3 or NPY, both 1:400, Thermo Fisher). Secondary antibody was dissolved in 1% normal donkey serum for PV or 1% normal goat serum for GluR2/3 or NPY ...
-
bioRxiv - Bioengineering 2023Quote: ... and detection antibodies goat anti-mouse-Alexa Fluor 647 (Cat. #A21240) and goat anti-rabbit-Cy3 (Cyanine 3, Cat. #A10520) were obtained from Fisher Scientific.
-
bioRxiv - Neuroscience 2023Quote: ... Heads were washed with PAT for 5 hours and then incubated for 3 days in PAT-NGS with goat anti-rabbit AlexaFluor-488 (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... then incubated for 3 days (room temperature during the day and 4 °C at night) in PAT-NGS containing rabbit anti-GFP (Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2023Quote: ... 10 mins) and resuspended in 3 mL MAGIC buffer supplemented with 60 µL Rabbit anti-Mouse IgM FITC Secondary Antibody (Invitrogen, 31557). The sample was gently rocked at 4 °C for 20 hrs ...