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Citations for Thermo Fisher :
51 - 100 of 10000+ citations for Retinol Binding Protein RBP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Isolation of RNA from the RNA-RBP complexes were accomplished with the addition of TRIzol™ Reagent (Invitrogen™, 15596018) followed by the Direct-zol™ RNA MiniPrep (Zymo Research Cat No ...
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bioRxiv - Biophysics 2021Quote: ... The SNAP-tag allows for covalent binding of O6-benzylguanine (BG) to the protein.[63] Protein expression was performed in Escherichia coli BL21(DE3) pRARE (Invitrogen) under T7 promoter with 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were buffer exchanged into binding buffer using a Zeba spin desalting column (Thermo Fisher), serially diluted at 2:3 v/v ratio and then incubated with DNA for 20 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Binding of recombinant proteins to pre-spotted PIP and membrane lipid strips (Thermo Fisher; P23751) was performed according to (Dowler et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein binding was detected with an Alexa488-conjugated anti-GST antibody (Life Technologies A-11131), and arrays were scanned using a GenePix 4400A (Molecular Devices ...
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bioRxiv - Microbiology 2023Quote: ... The high protein-binding capacity 96 well ELISA plate (Nunc MaxiSorp® flat-bottom, Invitrogen) was coated with ELISA capture antibody ...
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bioRxiv - Microbiology 2023Quote: ... The high protein-binding capacity 96 well ELISA plate (Nunc MaxiSorp® flat-bottom, Invitrogen) was coated with ELISA capture antibody ...
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bioRxiv - Molecular Biology 2023Quote: All samples were prepared in low protein-binding microcentrifuge tubes (ThermoFisher Scientific, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2023Quote: ... Collection tubes were replaced with 1.5 mL low protein binding tubes (Thermo Scientific; cat. 90410). To each column was added 0.25 µg Trypsin+Lys-C (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... Dissected tissues were then transferred to low protein binding microcentrifuge tubes (90410, Thermo Fisher Scientific) with 0.2-0.5µL of buffer accompanying the tissue ...
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bioRxiv - Biochemistry 2020Quote: A 5-mL HiTrap™ Protein G HP column (Cytiva) was equilibrated with 10 CVs of Protein G IgG Binding Buffer (pH 5.0; Thermo Fisher Scientific). Spent medium containing CR3022 mAb was diluted with binding buffer at a 1:1 volume ratio and filtered using a 0.22 μm stericup ...
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bioRxiv - Cell Biology 2023Quote: ... The RBP-bound RNAs were subjected to on-bead biotinylation using Pierce RNA 3’ End Biotinylation Kit (Thermo Fisher, cat#20160). After labeling ...
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bioRxiv - Microbiology 2024Quote: ... The RBP genes were either amplified by PCR from the phage genome or ordered as synthetic DNA fragments from Thermo Scientific via the Invitrogen GeneArt Synthesis Services in cases where the phage itself was unavailable as PCR template ...
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bioRxiv - Immunology 2021Quote: ... The media was diluted with an equal volume of Protein A IgG Binding Buffer (Thermo Scientific) and loaded onto the column ...
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bioRxiv - Physiology 2022Quote: ... 18S ribosomal RNA (18S) and TATA-binding protein (Tbp).The primers (Thermofisher Scientific, Waltham, MA, USA) were derived from mouse sequences and are listed in supplemental methods (supplement table 2.).
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bioRxiv - Molecular Biology 2021Quote: Putative ac4C binding proteins were subjected to SDS PAGE on Novex Tris-Glycine 4-20% (Invitrogen) gel followed by either silver staining with Pierce Silver Stain Kit (Thermo ...
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bioRxiv - Physiology 2019Quote: ... Tadalafil plasma protein binding was also determined by mass spectrometry following rapid equilibrium dialysis (ThermoFisher, UK). K2EDTA plasma samples (100 µl ...
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bioRxiv - Immunology 2021Quote: ... S protein binding was detected using 25 µL/well of PNPP (p-nitrophenyl phosphate;Thermo Fisher) and the reaction was measured at a wavelength of 405 nm by the Varioskan Lux Reader (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA-protein binding reactions were performed according to the manufacturer’s instructions (Thermo Fisher Scientific, USA). The 4% polyacryl gel was used to separate the DNA-protein complexes ...
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bioRxiv - Cell Biology 2020Quote: ... KKT4 proteins were buffer-exchanged into the binding buffer using Zeba spin desalting columns (Thermo Fisher). KKT42–645 (0.67 µM ...
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bioRxiv - Immunology 2021Quote: Nunc MaxiSorpTM high protein-binding capacity 96 well plates (Thermo Fisher Scientific, Cat# 44-2404-21) were coated overnight at 4 °C with 1µg/ml SARS-CoV-2 RBD ...
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bioRxiv - Bioengineering 2020Quote: ... The flavin binding fluorescent protein gene was synthesized from previously known sequence (56) (Thermo Fisher Scientific) and the plasmid was formed using the hot fusion method ...
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bioRxiv - Immunology 2022Quote: ... Supernatant with positive RBD binding signals was next purified using Protein A/G beads (Thermo Scientific), concentrated using a 30 kDa or 100 kDa cut-off concentrator (Vivaspin ...
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bioRxiv - Neuroscience 2022Quote: JD1 antibody was adsorbed to high protein binding Nunc-Immuno MaxiSorp 96-well plates (Nunc, Roskilde,) at 4°C for 4hrs ...
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bioRxiv - Cell Biology 2023Quote: All steps from here on were performed in Low protein binding microcentrifuge tubes (Thermo Scientific, 90410). Bead preparation and antibody binding ...
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bioRxiv - Cell Biology 2024Quote: ... Antibody binding was followed by incubation with Protein A/G magnetic beads (Thermo Fisher Scientific, USA) for 1h at 4°C on a rotator ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mM MgCl2) for cell membrane binding proteins (LPL, Caveolin 1) and quantified with Pierce™ BCA Protein Assay Kit (Buffer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: rox2RNA probes at 100 nM and hrp38 RNA probes at 50nM were incubated with MBP-tagged FL CLAMPWT protein or MBP-tagged FL CLAMPdelPrLD protein in REMSA binding buffer provided with the LightShift Chemiluminescent RNA EMSA kit (Thermo Scientific, USA) at room temperature for 30 min according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Platelet-poor plasma samples were then stored in low-protein binding tubes (Thermo Fisher Scientific, Waltham, USA) at −80°C ...
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bioRxiv - Biochemistry 2020Quote: ... Post-dye removal all samples were transferred to 1.5 mL low protein binding tubes (Thermo Fisher Scientific) with 1×109 200 nm Red FluoSpheres (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by binding of antigen antibody complexes to protein A/G magnetic beads (Thermo scientific, Rockford, USA) for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... Bound material was recovered by subsequent incubation of binding reactions with 10 mL Protein A Dynabeads (Invitrogen) coupled to 2.5 mg mouse anti-FLAG at 4°C for 1 h ...
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bioRxiv - Microbiology 2023Quote: DB1 antibody was purified by binding clarified cell supernatant to Protein A Agarose beads (Thermo Fisher Scientific), with elution into 100 mM glycine pH 3.0 and was quickly neutralised with 1 M Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... Next the columns were placed into fresh Low Protein Binding Microcentrifuge Tubes (Thermo Scientific, Waltham, MA, USA) to harvest tryptic peptides after digestion ...
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bioRxiv - Biochemistry 2023Quote: ... wild-type α-actin and 15 μM Pi-sensor (MDCC-labeled phosphate binding protein, Thermo Fisher Scientific) was prepared ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by insertion into each site of TTHA0766 lactate binding protein (LBD) in a pBAD vector (Life Technologies) by Gibson assembly (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... and the lipid-binding proteins were detected using the SuperSignal West Femto Maximum Sensitivity Substrate kit (ThermoFisher Scientific) and a ChemiDoc MP imaging system (Bio-Rad) ...
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bioRxiv - Plant Biology 2019Quote: ... The beads from 14N-labeled BIN2-TbID were transferred to new low protein binding tube (Thermo Fisher Scientific) and mixed with that of 15N-labeled YFP-TbID ...
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bioRxiv - Cell Biology 2021Quote: ... Resin was then washed 3x with binding buffer and protein was eluted with 4X LDS sample buffer (ThermoFisher). Samples were run on a Bis-Tris gel and stained with Coomassie Brilliant Blue R-250 (GolBio).
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bioRxiv - Cancer Biology 2022Quote: ... and 1 mM MgCl2 containing 4.5 µM phosphate-binding protein labeled with a phosphate sensor (MDCC, Fisher Scientific) for intrinsic GTPase activity or containing 1 nM NF1-GAP for GAP mediated GTPase activity ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight in polyclonal anti-RBPMS (RNA Binding Protein Multiple Partners; 1:1000; ThermoFisher, CAT: PA5-31231) at 4ºC ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell lystaes were then transferred to low-protein binding Eppendorf tubes (Cat. No. 13-698-794, Fisher Scientific) containing 3 μl of anti-REV-ERBα antibody ...
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bioRxiv - Biochemistry 2023Quote: ... Manufacturer B (Pierce™ Microcentrifuge Tubes no. 69715 and Low Protein Binding Microcentrifuge Tubes no. 90410, Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... using Lysis/Binding Buffer (Invitrogen), and frozen at −80◦ ...
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bioRxiv - Microbiology 2021Quote: ... Pierce™ white streptavidin-coated high binding capacity 96-well binding plates (ThermoFisher Scientific; 15502) were washed twice with ELISA wash buffer and coated with 5μg/ml of biotiylated peptides at room temp ...
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bioRxiv - Genetics 2021Quote: The sequence of the Vd_octβ2r gene described in this study was used to design primers (flanking the N87 and Y215 positions in the Vd_Octβ2R protein) and two minor groove-binding probes (MGB) (ThermoFisher Scientific) using the Custom TaqMan® Assay Design Tool (https://www.thermofisher.com/order/custom-genomic-products/tools/genotyping/) ...
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bioRxiv - Immunology 2021Quote: ... 18,000xg) and pre-cleared for non-specific binding with 40 ul BSA-blocked protein A/G magnetic Dynabeads (Invitrogen) for 4h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant proteins were affinity purified by overnight binding to HisPur Cobalt Resin (Thermo Fisher Scientific, U. S. A.) in buffer A (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Biochemistry 2020Quote: ... Binding of the full-length S protein was detected by mouse anti-His IgG (Invitrogen MA121315, 2 μg/ml) and Alexa Fluor-488-labelled goat anti-mouse IgG (Invitrogen A11001 ...
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bioRxiv - Neuroscience 2019Quote: ... Pellets were resuspended in binding buffer without protease inhibitors and protein concentration was measured using BCA assay (Thermo Scientific). Membranes were diluted to a 1.6μg/μl working concentration and stored at −80°C.