Labshake search
Citations for Thermo Fisher :
51 - 100 of 4028 citations for Influenza Virus Hemagglutinin HA H7N9 Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... PierceTM HA epitope tag antibody beads (Thermo Scientific, Rockford, IL, USA) were used to enrich HA-tagged MOR following the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... Protein products with HA tag were detected using 1:500 anti-HA antibody (Life Technologies, Catalog number: 326700), NURF-1.D isoform with FLAG tag was detected using 1:1000 PIERCE ANTI-DYKDDDDK antibody (Life Technologies ...
-
bioRxiv - Microbiology 2023Quote: ... The monoclonal HA-epitope tag antibody 2-2.2.14 (Invitrogen, catalog no. 26183) was used for detection of hemagglutinin-tagged proteins (1:5000 dilution) ...
-
bioRxiv - Developmental Biology 2023Quote: ... coated with 3 µg of HA-Tag monoclonal antibody (#26183, ThermoFisher Scientific) at 4°C for overnight ...
-
bioRxiv - Plant Biology 2024Quote: ... Sonicated chromatin was incubated with 2μg Anti-HA Tag Antibody (#26183, Invitrogen) for 6h at 4°C and an additional 2h at 4°C with 20μl Dynabeads Protein G (#10003D ...
-
bioRxiv - Neuroscience 2022Quote: Genes encoding the full-length sequences of Human PP2A A subunit and PP2A C subunit with N-terminal His tag and non-cleavable HA tag were sub-cloned into the pFastBac-Dual vector (Invitrogen). Sequences of PP2A A and C subunits are shown in Table 1 ...
-
bioRxiv - Biochemistry 2020Quote: The full-length cDNA for HA or a mutated gene and neuraminidase genes of influenza A/Switzerland/9715293/2013 (H3N2) virus were amplified by using SuperScript One-Step RT-PCR (Invitrogen, Grand Island, NY), and the 6:2 recombinant viruses with six internal genes of influenza A/PR/8/1934(H1N1 ...
-
bioRxiv - Microbiology 2023Quote: ... and real-time RT-PCR was performed to detect the presence of influenza A virus (M gene) using AgPath-ID™ One-Step RT-PCR Kit (Thermo Fisher Scientific). Positive influenza samples were then selected for whole genome sequencing using next generation sequencing (NGS ...
-
bioRxiv - Biophysics 2020Quote: ... Transmembrane protein constructs with extracellular HA-group were cross-linked with Biotin anti-HA.11 Tag Antibody (BioLegends) and Streptavidin Alexa Fluor 647 (Thermofisher).
-
bioRxiv - Microbiology 2020Quote: ... The HA-tagged proteins were captured with the Pierce™ HA-Tag Magnetic IP/Co-IP Kit (Thermo Scientific™). Magnetic beads (0.25 mg ...
-
bioRxiv - Developmental Biology 2022Quote: ... pMT-yki-HA was generated by cloning Yki-RG and HA tag into pMT-V5-His vector (Invitrogen, Cat# V412020). The Y281A ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... NK-CD16 cells with positive SmBiT expression were selected via cell sorting by probing HA tag (anti-HA antibody [Invitrogen #26183 ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Cells were incubated with 1:200 dilution of HA tag monoclonal antibody (Invitrogen) for 30 minutes at 4°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... containing an N-terminal HA epitope tag with an LR reaction (Life Technologies) per manufacturer’s instructions.
-
bioRxiv - Pharmacology and Toxicology 2023Quote: PierceTM HA epitope tag antibody was obtained from Thermo Scientific (Rockford, IL, USA). The rabbit polyclonal phosphosite-specific µ-opioid receptor antibodies anti-pT370 (7TM0319B) ...
-
bioRxiv - Cell Biology 2023Quote: The Pierce™ HA-Tag IP/Co-IP Kit (Thermo Fisher Scientific, USA) was used according to the manufacturer’s instructions but with minor modifications ...
-
bioRxiv - Biochemistry 2021Quote: ... Coverslips were washed three time with PBS before the incubation with Alexa Fluor 555 (for Myc tag) and/or Alexa Fluor 647 (for HA tag) conjugated secondary antibodies (Invitrogen/Thermo Fisher) diluted in PBSAT for 30 min at RT ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and the HA-tag was stained with 2.5 µg/mL (1:400) rabbit anti-HA (RM305) (Thermo Fisher, Cat.#MA5-27915) and 10 µg/mL (1:200 ...
-
bioRxiv - Cancer Biology 2020Quote: ... RELA with C-terminal HA tag into Gateway donor vector (pCR8/GW/TOPO, Invitrogen). Then LR clonase II reactions were used to shuttle ORFs into pCW-DEST (lentiviral Dox-inducible expression ...
-
bioRxiv - Cell Biology 2020Quote: ... with an N-terminal FLAG- or HA-tag (16) or into pcDNA4/TO (Invitrogen) with an N-terminal FLAG- or EGFP-tag (17) ...
-
bioRxiv - Microbiology 2021Quote: ... Membranes were probed with monoclonal HA Tag antibody (1:2000 dilution, Invitrogen, 2-2.2.14,), incubated with anti-mouse IgG-horseradish peroxidase (IgG-HRP ...
-
bioRxiv - Biochemistry 2022Quote: ... then stained with Invitrogen HA Tag Polyclonal Antibody (SG77) (Thermo Fisher Scientific 71-5500) and Purified anti-His Tag antibody (BioLegend 652502 ...
-
bioRxiv - Immunology 2024Quote: ... anti-HA epitope tag (#901513) and Alexa Fluor 647 anti-mouse IgG (Invitrogen #A21235).
-
bioRxiv - Biochemistry 2021Quote: ... Coverslips were washed three time with PBS before the incubation with Alexa Fluor 555 (for Myc tag) and/or Alexa Fluor 647 (for HA tag) conjugated secondary antibodies (Invitrogen/Thermo Fisher) diluted in PBSAT for 30 min at RT ...
-
bioRxiv - Molecular Biology 2020Quote: ... and rabbit anti-influenza A PB1 (Invitrogen, PA-34914 ...
-
bioRxiv - Plant Biology 2023Quote: ... Supernatants were mixed with 20 μL anti-HA-agarose beads and incubated overnight following the manufacturer’s instructions (Pierce™ HA-Tag IP/Co-IP Kit, Thermo Fisher Scientific). The HA-tagged protein was eluted and quantified by the Bradford method ...
-
bioRxiv - Microbiology 2023Quote: ... or mouse monoclonal antibody against hemagglutinin (Thermo Scientific cat # 26183) (1:5000 dilution) ...
-
bioRxiv - Cell Biology 2020Quote: ... Transfection efficacy was confirmed by western-blot measurement of the expression of the HA-tagged fusion proteins with the HA tag monoclonal antibody (Thermo Fisher Scientific, USA).
-
bioRxiv - Cancer Biology 2020Quote: ... an HA tag was added to IQGAP1 and cloned via Gateway PCR (Invitrogen, Carlsbad, CA) into a pT3-EF5α vector ...
-
bioRxiv - Microbiology 2019Quote: ... each fused to a C-terminal HA-tag using lipofectamine 3000 transfection reagent (ThermoFisher, L3000015) according to manufacturer instructions ...
-
bioRxiv - Genomics 2022Quote: ... the HA-tagged protein of interest was detected with 1:500 diluted HA Tag Alexa Fluor 647 conjugated mouse antibody (ThermoFisher, Catalog no. 26183-A647). After antibody incubation ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... NK-CD16 cells with positive SmBiT expression were selected via cell sorting by probing HA tag (anti-HA antibody [Invitrogen #26183, 1:500]; Anti-mouse secondary antibody Alexa Fluor 647 [Invitrogen #A-21235, 1:200]), probing CD16 (anti-CD16 antibody [Abcam #246222 ...
-
bioRxiv - Neuroscience 2022Quote: Antibodies used for these experiments include: anti-HA-tag (Mouse, ProteinTech, Thermo Fisher, 50-173-6449), phospho-TAOK2 (S181 ...
-
bioRxiv - Microbiology 2021Quote: ... monoclonal anti-HAtag antibody (1:8000; HA Tag mAb 2-2.2.14, Thermo Fisher Scientific, Planegg, Germany) or COVID-19 patient serum (1:200) ...
-
bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal anti-HA-Tag (16B12) Alexa flour-594 (Invitrogen, A21288, Lot: 2218838, IF: 1:100); mouse monoclonal anti-FLAG M2 (Sigma ...
-
bioRxiv - Neuroscience 2023Quote: Co-immunoprecipitation was performed using the Pierce Magnetic HA tag IP/Co-IP Kit (ThermoFisher, #88838). The manufacturer’s Manual IP/Co-IP protocol and the Elution Protocol using sample buffer were applied.
-
bioRxiv - Developmental Biology 2023Quote: ... The HA tag was inserted after the signal sequence of Fzd6 in the pCR2.1-TOPO (Invitrogen) vector by In-Fusion cloning ...
-
bioRxiv - Neuroscience 2024Quote: ... extracted protein was immunoprecipitated using 25μL prewashed HA Ab-Tag Magnetic beads (Thermo Fisher Scientific, Massachusetts) with gentle rotation at 4°C for 30min ...
-
bioRxiv - Biophysics 2021Quote: ... the protein was expressed as a fusion construct with an N-terminal His6 tag followed by tobacco etch virus (TEV) protease cleavage site in E.coli BL21 DE3 Star cells (Invitrogen). The fusion construct was purified using immobilized-nickel affinity chromatography ...
-
bioRxiv - Immunology 2022Quote: ... or mouse anti-Influenza NP (Cat#: MA5-29926; Invitrogen) diluted 1:2,000 in PBS ...
-
bioRxiv - Microbiology 2022Quote: ... FITC anti-Influenza A NP (Thermofisher, Cat# MA1-7322), PE anti-mouse CD45 (BioLegend ...
-
bioRxiv - Biochemistry 2024Quote: ... The membrane was then probed with horseradish peroxidase (HRP)-conjugated HA tag monoclonal antibody (2-2.2.14) (ThermoFisher) using 1:1,000 dilution and signal quantification was performed using ImageJ software ...
-
bioRxiv - Microbiology 2022Quote: ... Western blots were performed on the immunoprecipitated protein samples using the mouse-anti-HA tag (2-2.2.14) primary antibody (Invitrogen) (1:1,000 ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Tetherin localisation in cells was detected by staining cells with anti-HA-tag rabbit monoclonal IgG (Thermo Fisher) diluted in 0.2% Triton X-100 ...
-
bioRxiv - Microbiology 2023Quote: ... Protein accumulation was monitored in lysed overnight Cm cultures by western blot using HA Tag Monoclonal Antibody (2-2.2.14, ThermoFisher) as described by Sambrook and Russell ...
-
bioRxiv - Molecular Biology 2024Quote: ... FLAG M2 or HA-tag antibody at 4 °C overnight before precipitated with magnetic Protein G beads (Thermofisher) for 2 hours at 4 °C followed by 3 washes with RIPA buffer.
-
bioRxiv - Biochemistry 2024Quote: ... Reading frames from these pcDNA backbones with 3×HA tags were amplified and inserted into pcDNA4/TO (Invitrogen) containing a C-terminal 3×FLAG affinity tag using Gibson assembly ...
-
bioRxiv - Molecular Biology 2021Quote: The coding sequence of MHV-S Hemagglutinin Esterase inactive (S45A) (1218bp) fused to human IgG-Fc followed by a 6xHis tag in the mammalian expression vector pcDNA3.1(-) (Invitrogen) was kindly provided by Colin Parrish (MHV-S-HE_Fc_His6 ...
-
bioRxiv - Biophysics 2021Quote: Full-length HA-tagged p75NTR and full-length Myc-tag TRADD were expressed from a pcDNA3 vector backbone (Invitrogen). The HA-p75E345A ...
-
bioRxiv - Biophysics 2022Quote: The human wild-type S1PR5 gene (UniProt ID Q9H228) with an N-terminal 3 × HA epitope (YPYDVPDYA) tag was cloned into pcDNA3.1+ (Invitrogen) at KpnI(5’ ...