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Citations for Thermo Fisher :
9801 - 9850 of 10000+ citations for 6 Methyl 2 Mercaptobenzothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The cells were transiently transfected with plasmids expressing GSPT1-HA wild type and variants (6 µg) using lipofectamine 3000 according to manufacturer’s protocol (Thermo Fisher Scientific). After 48 h ...
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bioRxiv - Microbiology 2021Quote: ... Culture dishes (15cm) coated with poly-L-lysine were plated with 6×106 neurons each and cultures maintained in Neurobasal media (Thermo Fisher) supplemented with GlutaMAX (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: Viral RNA was isolated from buccal swabs collected on dpi 6 and stored in 500 µl of TRIzol™ Reagent (Invitrogen). Total RNA extractions from buccal swab samples were performed using an adapted TRIzol™ manufacturers protocol with a 45 min precipitation step at −20° C ...
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bioRxiv - Neuroscience 2021Quote: Lateral ventricles containing the adjacent striatum was collected from 6 week WT and Nrmt1-/- mice and total RNA was lysed and extracted using TRIzol (Life Technologies) and chloroform ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were seeded at a density of 2.5 x 105 cells/well in a 6-well dish and transfected with CRISPR-Cas9 plasmids using the standard Lipofectamine 3000 (Thermo Fisher) protocol ...
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bioRxiv - Physiology 2021Quote: ... the libraries were quantified using a Collibri library quantification kit (Thermo Fischer Scientific, Ref. A38524100) in a QuantStudio 6 Flex (Life Technologies).
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bioRxiv - Pathology 2021Quote: ... qPCR analysis of gene expression was performed using QuantStudio 6 Real-Time PCR System and SYBR Green PCR Master Mix (Applied Biosystems). Analysis of relative gene expression was carried out using the comparative CT method (ΔCT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... ExpiFectamine CHO Enhancer (6 μl per ml of culture) and ExpiCHO Feed (240 μl per ml of culture) (Thermo Fisher Scientific) were added 18-22 hours post-transfection and the temperature was decreased to 33 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time quantitative PCR was performed using a QuantStudio 6 Flex real-time PCR system (Applied Biosystems, Foster City, California USA) following manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2021Quote: ... Peptides were eluted along a 90 min gradient from 6 to 40% Buffer B (see StageTip purification) with the EASY□nLC 1,200 system (Thermo Fisher Scientific). Measurement was done on an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... PC12 cells were plated in 6 well dishes and transfected 48h prior to lysis with 45 pmol siRNA using Lipofectamine RNAiMAX (Thermo Fisher); cells were lysed in RIPA buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were washed once by centrifugation (80 g for 6 min) and resuspended in complete Minimum Essential Medium (MEM; Thermo Fisher) supplemented with 5 % FBS (Thermo Fisher) ...
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bioRxiv - Immunology 2020Quote: ... or empty vector control GFP+ cells (n=6) using TRIzol reagent according to manufacturer instructions (Thermo Fisher Scientific, Waltham, MA, USA). Integrity of total RNA was checked using Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Twelve micrograms of peptides were labelled with TMT labels (6-plex) according to the manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA) and pooled ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the thermal cycling conditions ...
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bioRxiv - Immunology 2020Quote: Ferric Hb and ferrous Hb were individually biotinylated using sulfo-NHS-LC-Biotin (Sulfosuccinimidyl-6- {biotin-amido} hexanoate; Thermo Fisher Scientific) and immobilized on streptavidin beads in spin columns (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Capture antibodies were anti-IL-6 (14-7061-85, eBioscience), anti-TNF-α (14-7423-85, eBioscience) and anti-IL-1β (14-7012-81, Invitrogen). Detection antibodies conjugated with biotin were anti-mouse-IL-6 (554402 ...
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NVX-CoV2373 vaccine protects cynomolgus macaque upper and lower airways against SARS-CoV-2 challengebioRxiv - Immunology 2020Quote: ... Reactions were carried out on a Quant Studio 6 and 7 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA) according to the manufacturer’s specifications ...
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bioRxiv - Immunology 2020Quote: ... The MMP Luminex panel (PPX-05/ PROCARTAPLEX MMP1, MMP2, MMP3, MMP9 and MMP13 plex) and IL-6 Elisa (both from Life Technologies) was performed on co-cultures supernatants.
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bioRxiv - Immunology 2021Quote: ... The plate was then washed 6 times and 45uL of Phycoerythrin (PE)-conjugated goat anti-mouse IgG was added (ThermoFisher, 31861) and incubated shaking at 700RPM at RT for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... was performed on the Applied Biosystems QuantStudio 6 Flex Real-Time PCR System using Fast Universal PCR Master Mix and TaqMan Gene Expression assays (Applied Biosystems), listed in Table S1.
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bioRxiv - Immunology 2021Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the thermal cycling conditions ...
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bioRxiv - Genetics 2019Quote: ... Cells were transfected at 60-70% of confluence in 6-well plates using Lipofectamine® 2000 DNA transfection reagent (Invitrogen®) in Opti-MEM according to manufacturer’s instructions with 2µg of each NOVA2 plasmid ...
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bioRxiv - Genomics 2021Quote: ... Relative RNA expression was examined by qRT-PCR using a SYBR Green Master Mix (VAZYME, China) on the ABI QuantStudio 6 system (Applied Biosystems, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... followed by 10,000xg for 20 min in 500 ml vessels using a fixed angle FIBERlite F14-6 x 500y rotor (Thermo Fisher Scientific). The supernatant fraction was then centrifuged onto a 60% sucrose cushion in buffer A (10 mM HEPES pH 7.4 ...
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bioRxiv - Plant Biology 2020Quote: ... quantitative real-time PCR (qRT-PCR) assays were performed in the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, USA) using TB Green Premix Ex Taq II (Takara ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... interleukin-6 and -8 (IL-6 and IL-8) human Instant Elisa™ kits and matrix metalloproteinase-1 (MMP-1) human Elisa kit from ThermoFisher Scientific.
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bioRxiv - Microbiology 2020Quote: COS-7 cells were seeded onto 6-well plates 24 hours prior to the transient transfection using Lipofectamine 2000 reagent (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The same amount of protein lysate was aliquoted into different PCR tubes and simultaneously subjected to 6 different temperatures (37, 41, 45, 49, 53, 57 °C) for 3 min in the Veriti Thermal Cycler (Applied Biosystems). After 3 min cooling on ice ...
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bioRxiv - Neuroscience 2022Quote: MC4R enhancer knockout cells were generated by transfecting WTC11-NGN2 iPSC cells in 6-well plates with 6.25ug Cas9 protein (Fisher Scientific, A36498) and 800ng sgRNAs (IDT) ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were isolated from wildtype adult C57BL/6 mouse spleen and lymph nodes using the Dynabeads FlowComp Mouse Pan T (CD90.2) Kit (Thermo Fisher Scientific) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected using Lipofectamine 3000 kit (7.5 µl Lipo 3000 and 5 µl P3000 reagents per 6-well; Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted and quantified from WwoxWT/WT and WwoxP47T/P47T n=6 mice/group (3 males and 3 females) and cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were expanded to 6 × 106/mL the day before transfection and were then diluted to 3 × 106/mL by ExpiCHO™ expression medium (Gibco). Plasmids were diluted to 20 μg/mL by cold OptiPRO medium (Gibco ...
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bioRxiv - Biochemistry 2022Quote: AML12 cells were seeded at 1×105 cells/well in a 6-well plate and transfected with siRNA mixture (f.c. 10 nM) using Lipofectamine 2000 (Invitrogen, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed using inventoried TaqMan assays for respective target genes and housekeeping control genes (18S) on the QuantStudio 6 Flex Real-Time PCR System (Life Technologies). Fold change in gene expression was analyzed using the delta delta Ct method.
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bioRxiv - Biochemistry 2022Quote: ICabs A4 and B7 were labelled with NHS-Rhodamine (5/6-carboxy-tetramethyl-rhodamine succinimidyl ester, ThermoFisher Scientific (catalogue no. 46406)) as described by the manufacturer ...
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bioRxiv - Bioengineering 2022Quote: ... The quantitative real-time PCRs were performed on the QuantStudio 6 Flex Feal-Time PCR System with PowerUp SYBM Green Master Mix (Thermo Fisher). GAPDH served as the housekeeping gene ...
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bioRxiv - Biochemistry 2022Quote: ... Total protein extracts were prepared from 10 OD600 of cells by TCA lysis method and analyzed by Western blot after 6% SDS-PAGE (Novex Tris-Glycine, Thermo Fisher) or 7% Zn2+-Phos-tag™ SDS-PAGE according to the manufacturer’s protocol (20 µM Phos-Tag™ ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were dehydrated in 95% alcohol and stained with brand OG-6 solution (from 22-050-211, Thermo Fisher Scientific, USA) for 2 minutes and washed with 95% alcohol ...
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bioRxiv - Developmental Biology 2022Quote: The Tregs and the CD4+/CD25- T cells were isolated from C57BL/6 mouse spleen using the Mouse CD4+CD25+ Treg Cells Kit (Invitrogen 11463D). The CD4+/CD8+ cells were sorted from testis ...
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bioRxiv - Cell Biology 2022Quote: ... 5×105 cells were seeded in 6 cm plates and transfected with siRNAs using Lipofectamine™ RNAiMAX Transfection Reagent (#13778030, Invitrogen) as per manufacturer protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... TNF alpha mouse high sensitivity ELISA kit and IL-6 mouse high sensitivity ELISA kit were from Invitrogen (Thermo Fisher Scientific), mouse CCL2/JE/MCP-1 quantikine ELISA kit ...
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bioRxiv - Genetics 2022Quote: ... Quantitative expression analysis of Nos1ap was performed using mouse-specific TaqMan Gene Expression assay (Mm01290688_m1; mapping to exons 5 and 6) (Applied Biosystems, MA). Real-time qPCR was performed on a 7900HT Fast Real-Time PCR System or QuantStudio 5 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genetics 2022Quote: ... ~1 × 105 hTSC were seeded onto 6 well plates coated with either 5–10 μg/mL collagen IV (10376931, Fisher Scientific) or 0.5 μg/mL iMatrix 511 (NP892-011 ...
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bioRxiv - Genetics 2022Quote: ... U2OS-mBMAL1-dLuc-Puro (referred to as: Bmal1- dLuc) cells were maintained at 37°C and 6% CO2 in 25 mM (high) glucose DMEM (Thermo Fisher # 11995-065 with 1 mM sodium pyruvate ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was added to cDNA and the DNA was denatured at 95°C for 3 min prior to loading in Novex 6% TBE-urea gel (Thermo Fisher). Samples were separated for 45 min at 180 V ...
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bioRxiv - Microbiology 2022Quote: Total RNA from mock- or virus-infected (MOI=0.01) Vero E6 cells (106 cells/well, 6-well plate format) was extracted with TRIzol Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was digested with BsiWI and ligated in frame with a N-terminal 3 x FLAG-tag and a C-terminal 6 × histidine tag into a modified and BsiWI-digested pET101/D-TOPO expression vector (Thermo Scientific), yielding pET101-NanH1.
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bioRxiv - Molecular Biology 2022Quote: ... The products were analyzed in a denaturing 6% polyacrylamide gel with a 5’-labeled RiboRuler Low Range RNA Ladder (Thermo Scientific) as size markers ...