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Citations for Thermo Fisher :
9751 - 9800 of 10000+ citations for 6 Methyl 2 Mercaptobenzothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Adenovirus containing the MCMV IE1 gene was generated by transfecting purified pAd5-MCMV_IE1 into 293A cells in 1 well of a 6 well plate using Lipofectamine 3000 (ThermoFisher, Waltham, MA) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... L2 fibroblasts were seeded at 1.5 x 106 cells per well in 6-well tissue culture-treated plates containing collagen-coated glass coverslips (Thermo Fisher Scientific) and inoculated with L ...
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bioRxiv - Pathology 2022Quote: ... The iPSCs were continuously cultured and expanded on Geltrex coated 6-well plates with Essential 8 medium (ThermoFisher Scientific, Cat#A1517001). Human iPSC cell lines used in this manuscript ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were centrifuged at 135 x g for 1 minute and resuspended in 6 ml of hepatocyte media (William’s E media (Gibco, 22551-022) with 3% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... 6) The enriched fraction was pre-cleared twice by adding 60 μL equilibrated DEPC-treated Protein A Dynabeads (ThermoFisher Scientific 10001D) and nutating at 4°C for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... and the wells were washed five times with wash buffer before the addition of 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS, #37615, Thermo Fisher Scientific). Optical density was read 40 min later at 405 nm using a plate reader (SpectraMax i3 ...
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bioRxiv - Cancer Biology 2019Quote: ... VM-CUB1 and RT-112 cells were seeded at 1 × 105 cells per well in 6-well plates along with siRNA duplexes or miRNAs using Lipofectamine RNAiMAX Reagent (Life Technologies). At 72 hours after transfection ...
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bioRxiv - Microbiology 2019Quote: Cells were cultured in 6-well plate to 90% confluency and total RNA was extracted by Trizol (Life Technologies, Carlsbad, CA) and RT-qPCR was performed following the instruction of the manufacturer ...
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bioRxiv - Pathology 2019Quote: ... EB’s were returned to the ultra-low 6 well plate and transferred to Stage II media (DMEM, 15% KnockOut Serum Replacement (KOSR; Thermo Fisher), 1% NEAA ...
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bioRxiv - Neuroscience 2019Quote: ... Residual genomic DNA was removed from each of 6 biological replicates using the TURBO DNA-free™ Kit (Life Technologies, AM1907) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 50 μg of enriched mitochondria were resolved approximately 6 cm into a pre-cast 4–12% Bis-Tris polyacrylamide gel (ThermoFisher Scientific). The lane was excised and cut in 8 approximately equal chunks and the proteins reduced ...
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bioRxiv - Cell Biology 2019Quote: ... RNA was isolated from whole eyes of 6-month old cep290 mutant and sibling control animals (n = 9 per group) with Trizol (ThermoFisher 15596026). Reverse transcription using 1 μg RNA was performed with the iScript cDNA Synthesis kit (Bio-Rad 1708891) ...
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bioRxiv - Synthetic Biology 2020Quote: ... 2 µL of 1.25 µM 6-FAM-labeled ADO-22 primer and 1 µl of 100 mM ddTTP (USB-Affymetrix, Cleveland, OH). Fluorescently-labeled cDNA fragments were analyzed by capillary electrophoresis (CE) ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR was performed in a final volume of 10 μL per reaction in 384-well PCR plates using a thermocycler (QuantStudio 6 Flex, Applied Biosystems). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR was performed in a final volume of 10 μL per reaction in 384-well PCR plates using a thermocycler (QuantStudio 6 Flex, Applied Biosystems). Briefly ...
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bioRxiv - Immunology 2019Quote: Fabs from rabbits were expressed in 293F cells for 6 days and then affinity purified using a CaptureSelect™ CH1-XL Pre-packed 1 ml Column (ThermoFisher).
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bioRxiv - Zoology 2020Quote: ... each immunoblot lane represents a cell lysate produced from an entire well of a 6-well cell-culture dish (Thermo Scientific, Nunc). Lyates were diluted with SDS loading dye (final concentration ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293t were replated every 3 days at a split ratio of 1 in 6 following dissociation with Trypsin 0.05% EDTA (Gibco, Life technologies).
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bioRxiv - Molecular Biology 2019Quote: ... cells were seeded on a 6-well plate and transfected with 10 pmol of siControl (negative control #4390843, Ambion Silencer Select), siMCM2 (#s8536 ...
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bioRxiv - Physiology 2020Quote: ... The cells were transiently transfected for 16-24 hours with either human or mouse orthologs of TRPA1 using Fugene 6 transfection reagents and Opti-MEM (Thermofisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... hiPSCs at ~ 70% confluence (1.5×106 cells / well of a 6-well plate) were incubated in EDTA (Life Technologies #15575-020) for 4 min at room temperature (RT) ...
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bioRxiv - Microbiology 2020Quote: ... The synthesized coding DNAs with a 6-His-tag expressed at the C-terminus were cloned into the pPinkα-HC expression vector (Thermofisher, US) using XhoI/KpnI restriction sites ...
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bioRxiv - Microbiology 2021Quote: ... samples were unfrozen and aliquots of 6 ml per sample were fixed with Electron Microscopy Sciences 4% Paraformaldehyde (5025999, Fisher Scientific), stained with SYBR™ Gold Nucleic Acid Gel Stain (S11494 ...
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bioRxiv - Microbiology 2021Quote: ... in 6 well multi-dish Nunc UpCell Surface cell culture plates or 10 cm Nunc UpCell Surface cell culture dishes (Thermo Fisher) and differentiated to human monocyte-derived macrophages (hMDMs ...
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bioRxiv - Microbiology 2021Quote: Total RNA from virus infected (MOI=0.01) Vero E6 cells (106 cells/well, 6-well plate format) was extracted with TRIzol Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Barcoded beads were resuspended in lysis buffer (200 mM Tris-HCl pH8.0, 20 mM EDTA, 6% Ficoll PM-400 (GE Healthcare/Fisher Scientific), 0.2% Sarkosyl (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 60°C for 1 min.) using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). A dissociation curve was generated afterwards by ramping the temperature from 60°C to 95°C to determine the specificity of the PCR product ...
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bioRxiv - Genetics 2020Quote: ... followed by 35 cycles at 95 °C for 15 seconds and 30 seconds of 61.5 °C using a QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher). Three technical replicates were performed for each cDNA sample ...
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bioRxiv - Immunology 2020Quote: ... qPCR was performed in a final volume of 10□μL per reaction in 384-well PCR plates using a thermocycler (QuantStudio 6 Flex, Applied Biosystems). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... 500 000 cells per well were seeded in a 6-well-plate in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco/Thermo Fisher, France) with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... and 9.25 µL sterile water using the standard Power SYBR Green PCR Master Mix RT-PCR Protocol (Protocol Number 436721) on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher). As positive controls for the WSP and WPCP primer sets ...
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bioRxiv - Immunology 2021Quote: ... Cytokine concentrations in the day 6 supernatants of antigen-stimulated cultures were assessed by Luminex bead-based assay (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 5 x 105 HCT116 cells were seeded in a 6 well plate with Dulbecco’s modified Eagle medium (DMEM, Gibco®, Life Technologies) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2020Quote: 3×105 HeLa cells were seeded to each well of 6-well plates and cultured in DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Life Technologies) 24 hours before transfection ...
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bioRxiv - Physiology 2019Quote: ... Gene expression analysis was then performed by quantitative PCR (qPCR) on a Quantstudio 6 Flex Real-time PCR System (Applied Biosystems) using the UBR5 mouse primers in Table 1 (also used in the other mouse bioengineered muscle analysis) ...
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bioRxiv - Cell Biology 2019Quote: ... was used to amplify two homology arms spanning exons 5 and 6 of PPP2R1A and cloned into a bacterial artificial chromosome (Life Technologies). The homology arms were cloned into vector pNX (Papi et al. ...
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bioRxiv - Genetics 2020Quote: ... All organoids were passaged and split once a week 1:6 and filtered through a 40 micron cell strainer (ThermoFisher scientific) to remove differentiated structures from the culture.
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bioRxiv - Genetics 2020Quote: ... Cell lines were either used within 6 months after purchase or were periodically authenticated by microsatellite fingerprinting (AmpFLSTR Identifiler, Thermo Fisher) by the Cancer Genomics Research Laboratory/NCI) ...
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bioRxiv - Immunology 2021Quote: ... and BMDCs were incubated at a ratio of 10 microspheres per cell for 6 h with 1.0-μm FluoSpheres® carboxylate-modified microspheres (ThermoFisher, F8851) labelled with a red fluorescent dye (580 nm excitation and 605 nm emission) ...
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bioRxiv - Neuroscience 2021Quote: ... Abi3-WT and -KO MØPs were maintained in 6-well plates with Roswell Park Memorial Institute 1640 (RPMI; Gibco®, ThermoFisher) supplemented with 10% heat-inactivated Foetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2019Quote: ... then further cleared by centrifugation at 4°C and 25658 g for 1 h (Thermo Fisher Scientific, #A23-6×100 rotor). The supernatant was passed through a 0.22 µm filter (Corning #431097 ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: Transfection of sgRNA-Cas9 plasmids into Calu-6 cells for individual gene validation was done using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific), according to manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Each individual leaf tissue sample was processed in a plastic extraction bag using a HOMEX 6 homogenizer (BioReba, Swizerland) with 2.25 ml of TRIzol reagent following the manufacturer’s protocol (Thermo Fisher Scientific, USA). Concentration of the resulting RNA preparation was measured with a NanoDrop micro-volume spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... cells were cultured on gelatin-coated 6-wells in 60% BRL conditioned medium to 70-90% confluency and transfected with Lipofectamin3000 (Invitrogen L3000008) according to the supplier protocol with 2 μg donor vector and 1 μg Cas9/guide vector ...
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bioRxiv - Biochemistry 2020Quote: ... 500 mM 6-aminohexanoic acid) was added to lysates right before loading samples into a NativePAGE Bis-Tris gel 4-16% (Life Technologies). The proteins were separated according to Wittig et al.32 and transferred onto a PVDF membrane using a standard Tris-glycine transfer buffer with 0.05% SDS ...
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bioRxiv - Neuroscience 2020Quote: ... Culture were maintained in 6-,12- or 24-well plates in growth media consisting of 45% advanced DMEM/F12 solution (Gibco, # 126340010), 45% NBM solution (Miltenyibiotec ...
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bioRxiv - Bioengineering 2021Quote: ... hESCs were seeded at a density of 25×103 cell/cm2 on Matrigel-coated 6-well plates in Essential 8 medium (ThermoFisher, USA) on day −1 ...
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bioRxiv - Cancer Biology 2021Quote: ... and mPRs (PAQR5, 6, 7, 8, 9) using Power SYBR Green Master Mix with ViiA 7 Real-Time PCR System (Applied Biosystems). RT-qPCR plates with various cell-lines were prepared using an epMotion 5075 automated liquid handling system (Eppendorf) ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems). The following thermal cycling conditions were used ...
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bioRxiv - Cancer Biology 2021Quote: ... The fluorescent reaction was measured every 40 sec for 8 min and subsequent every 5 min for 6 times using a microplate reader (Thermo Scientific) with enzyme kinetics model ...