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Citations for Thermo Fisher :
9701 - 9750 of 10000+ citations for Recombinant Human ITGAL & ITGB2 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were clarified by centrifugation at 4°C and the protein concentration was determined by BCA protein assay (Thermofisher). Samples were resolved on a precast 4%–12% gradient gel (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... Protein concentrations were determined with a BCA assay (Pierce™ BCA Protein Assay Kit; #23225, ThermoFisher, Carlsbad, CA, USA). Total protein (5∼15 µg ...
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bioRxiv - Systems Biology 2023Quote: ... Protein concentrations in the pellet were determined using a Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, MA). After mixing 400 μL of supernatant with 400 μL of chloroform and 320 μL of water ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration in the extract was determined using a BCA protein assay kit (Thermo Fisher Scientific, cat. no. 23225) and adjusted to 2 μg/μL using the IP lysis buffer.
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bioRxiv - Neuroscience 2023Quote: ... Protein content of lysates was quantified using the PierceTM Protein Assay Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA). 20 µg of the protein samples were then heat-denatured and subsequently separated on a 4-12% Bis-Tris gel (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and measured for protein concentration using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: Protein concentration in the samples was determined with a BCA assay (Pierce BCA Protein Assay Kit; Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 6000 rpm 4°C and protein concentration was estimated by BCA protein assay kits (Thermo-Fisher Scientific, Cat #23225).
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bioRxiv - Microbiology 2024Quote: ... The concentrations of the protein extracts were determined using a BCA assay (Pierce BCA Protein Assay, Thermo Fisher Scientific) and a verification of the protein extracts was carried out on a 12.5% acrylamide SDS-PAGE gel stained with Coomassie brilliant blue R-250 ...
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bioRxiv - Cancer Biology 2024Quote: ... The total protein concentration of each cell lysate was determined with a BCA protein assay kit (Thermo Fisher, # 23225). For each sample ...
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bioRxiv - Cell Biology 2024Quote: ... The clarified protein samples were incubated with NeutrAvidin® Agarose resin (50 µL per 1 mg protein, Thermo Scientific) for 2 hours at RT ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of the protein was estimated using the BCA kit (Pierce BCA Protein Assay Kit - Thermo Fisher Scientific) according to the supplier’s recommendations as well as by comparing the protein on a 12.5% acrylamide Coomassie-stained SDS-PAGE gel with BSA standards ...
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bioRxiv - Synthetic Biology 2024Quote: ... Total protein content was quantified using PierceTM BCA protein assay kit as per the supplier’s protocol (Thermo Scientific, USA). For albumin ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 µl (for ChIP) or 100 μl (for ChIP-seq) Dynabeads Protein A and Protein G (Thermo Fisher Scientific) were pre-incubated overnight with rotation in the presence of 5 mg/ml BSA and 3 µg (for ChIP ...
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bioRxiv - Bioengineering 2024Quote: Protein samples were diluted in PBS to 5 μM and mixed with 5000× SYPRO Orange Protein Gel Stain (Invitrogen) to final 1.25x concentration ...
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bioRxiv - Molecular Biology 2024Quote: ... and the resulting supernatant was assayed for protein concentration with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and then subjected to SDS-polyacrylamide gel electrophoresis on a 10% acrylamide/4% bisacrylamide gel ...
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bioRxiv - Cell Biology 2024Quote: ... Peak measurements were normalized to protein content of duplicate samples as determined by BCA protein assay (Thermo Fisher Scientific). All experiments were performed independently at least three times and a representative experiment is shown.
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bioRxiv - Neuroscience 2024Quote: ... Supernatants were collected and the total amount of proteins was calculated by the BCA Protein Assay kit (ThermoFisher Scientific) using a BSA standard curve ...
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bioRxiv - Neuroscience 2024Quote: ... the samples underwent normalization based on protein content using the BCA protein assay kit (#23225, Thermo Scientific Pierce, US) per the manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... and total protein concentrations of cleared cell lysates were measured with the PierceTM BCA Protein Assay Kit (ThermoFisher Scientific) using BSA as a standard ...
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bioRxiv - Neuroscience 2024Quote: ... Total protein levels in all lysates were quantified using PierceTM BCA Protein Assay Reagents (Thermo Fisher: #23222 and #23224). After the incubation step for the poly-GP immunoassay ...
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bioRxiv - Microbiology 2024Quote: ... The protein concentration in each sample was determined using the BCA protein assay kit (Thermo Fisher Scientific, Waltham, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and total protein concentrations were quantified using a Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA). Samples for gel electrophoresis were prepared by boiling in loading buffer for 10 min ...
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bioRxiv - Systems Biology 2024Quote: ... The protein concentrations in both supernatants (SOL and SDS) were determined with Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... Lysate was clarified by centrifugation and total protein concentration was measured with the BCA Protein Assay (Thermo Fisher Scientific). Samples were electrophoresed and transferred to a 0.45 μm nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein were supplemented with 150mM NaCl and incubated with Protein A/G Magnetic Beads (ThermoFisher #88802) preloaded either or not with Golgin-97 antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysate was clarified by centrifugation and total protein concentration was measured with the BCA Protein Assay (Thermo Fisher Scientific). Samples were electrophoresed and transferred to a 0.45-μm nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: Human melanocytes and melanoma cells were lysed in Pierce RIPA buffer (Thermo Scientific) containing 1× Halt protease inhibitor cocktail (100× ...
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bioRxiv - Cancer Biology 2021Quote: ... Human CRC organoids medium is composed of Ad-DF+++ (Advanced DMEM/F12 (GIBCO) supplemented with 2 mM Ultraglutamine I (Lonza) ...
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bioRxiv - Cell Biology 2020Quote: Primary human umbilical vein endothelial cells (HUVECs) were obtained from Gibco (Cat#: C0035C) and cultured in Medium 200 (Cat# ...
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bioRxiv - Cell Biology 2020Quote: ... HIF1A levels were measured in duplicates applying a HIF1A Human ELISA kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... plate was incubated with HRP conjugated anti-human IgA (1:1000, Thermo Scientific) for 1hour in TBST with 1% non-fat dry milk ...
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bioRxiv - Cell Biology 2020Quote: Human induced pluripotent stem cells (hiPSCs) were cultured in StemFlex media (Gibco, #A3349401) and passaged with EDTA (Life Technologies #15575-020) ...
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bioRxiv - Immunology 2021Quote: ... HRP conjugated Rabbit anti-Human IgG(H+L) (Cat. No. A18903, Invitrogen, USA) as added to each well as the detection antibody ...
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bioRxiv - Genetics 2019Quote: ... RPE1 cells and primary human fibroblasts were cultivated in complete DMEM medium (Gibco).
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bioRxiv - Molecular Biology 2021Quote: Human MSCs were maintained in growth media (Dulbecco’s Modified Eagle Medium (DMEM, Gibco) containing D-glucose (1g/L) ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with human keratinocyte growth supplement (catalogue #S-001-5, Thermo Fisher Scientific) and 1% penicillin/streptomycin (catalogue #15140-122 ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were constructed using the Ion AmpliSeq Transcriptome Human Gene Expression kit (Thermofisher). Templating and sequencing were performed with the Ion PI HiQ OT200 kit ...
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bioRxiv - Immunology 2021Quote: ... Anti-human Perforin eF450 (1:100, clone dG9, Invitrogen, cat # 48-9994-42). Cells were analyzed on a BD LSR Fortessa flow cytometer ...
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bioRxiv - Immunology 2021Quote: For polyreactivity ELISAs against human insulin (MilliporeSigma) and dsDNA (Calf Thymus DNA; Invitrogen), plates were coated with 2µg/ml and 50µg/ml ...
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bioRxiv - Immunology 2021Quote: After 48 h of stimulation with Human T-activator CD3/CD28 Dynabeads (ThermoFisher) CD4+ lymphoblasts were recovered for transfection of CRISPR/Cas9 nucleoprotein complexes ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... and 10 µl/ml Fibronectin from human plasma (Thermo Fisher Scientific, #33016-015)] for 2-3 h at 37 °C/5% CO2 and stored at 4 °C after thorough removal of coating solution.
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bioRxiv - Physiology 2019Quote: Human embryonic kidney (HEK-293) cells were cultured in DMEM (Gibco, Basel, Switzerland) supplemented with 10% FBS ...
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bioRxiv - Genomics 2019Quote: We used GeneChip Human Exon 1.0 ST arrays (Affymetrix, Santa Clara, CA, USA) to measure the expression levels of transcripts in each brain tissue specimen ...
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bioRxiv - Cell Biology 2019Quote: ... Human M-CSF and Ficoll histopaque were purchased from Invitrogen (Carlsbad, CA, USA). Human CD14 magnetic beads were obtained from Miltenyi Biotec (Auburn ...
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bioRxiv - Cancer Biology 2019Quote: ... Mouse and human PSCs were cultured in DMEM (10-013-CV; Fisher Scientific) containing 5% FBS ...
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bioRxiv - Immunology 2019Quote: ... and sCD163 (Thermo Scientific™ Pierce™ Human CD163 Kit, Thermo Scientific, USA) in plasma.
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bioRxiv - Immunology 2020Quote: ... Human embryonic kidney (HEK293T) cells and HeLa cells were cultured in DMEM (Gibco) supplemented with 100U/ml Penicillin (Sigma-Aldrich),100μg/ml Streptomycin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... T Cells were stimulated with Dynabeads™ Human T-Expander CD3/CD28 (Gibco). The cells were cultured in GRex (Wilson Wolfe ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... Cells were incubated with AF488 goat anti-human IgG1 (1:500, A10631, Invitrogen) for 30 min at 4°C ...