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Citations for Thermo Fisher :
9651 - 9700 of 10000+ citations for Recombinant Human ITGAL & ITGB2 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Supernatant was collected and protein quantification was done with BCA reagent (Thermo Scientific Pierce BCA Protein Assay Kit, PI23227). 20 μg of each sample was separated on 8% SDS-PAGE followed by transfer onto a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the clarified supernatant was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: The protein concentration of EV enriched fractions was quantified by Pierce BCA Protein Assay Kit (Thermo Scientific, Waltham, MA) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The total protein content was analyzed by biochinonic acid-assay (Pierce™ BCA Protein Assay Kit, Thermofisher Scientific, USA). The total protein content of the NTHi lysate was adjusted to 2.5 mg/ml ...
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bioRxiv - Immunology 2023Quote: ... Lysates were collected and protein concentration was measured using the Pierce BCA Protein Assay Kit assay (Thermo Scientific #23225) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... separating proteins by electrophoresis with Chameleon Pre-Stained Protein Ladder (Li-Cor) on 4–12% Bis-Tris Gels (Invitrogen) at 150 V for 90 min ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was collected and total protein was quantified using Pierce BCA protein assay kit (Cat# 23225, Thermo Fisher Scientific). A 96 well plate was coated with 2 µg/mL spike antibody overnight at 4°C (Cat# 41050-D003 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Both measurements had CV% < 10 and are expressed per mg of protein (measured via BCA protein assay, ThermoFisher Scientific), following Espin et al ...
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bioRxiv - Biochemistry 2023Quote: ... The total protein concentration from the lysate was measured by Rapid Gold BCA protein assay kit (Thermo Scientific A53225) to normalize the amount of total protein in each sample ...
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bioRxiv - Biochemistry 2023Quote: ... The protein content of the cleared lysates were quantified using Pierce Rapid Gold BCA Protein Kit (Thermo Scientific, A53226) and separated by Bio-Rad TGX stain-free gel ...
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bioRxiv - Plant Biology 2022Quote: ... The gel was re-used for protein quantification using SYPRO Ruby Protein Gel Stain (Themo Fisher Scientific, Loughborough, UK) following manufacturer’s instructions and imaged using a UV transilluminator (Ultra-Violet Products Ltd ...
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bioRxiv - Cancer Biology 2023Quote: ... The total amount of protein was quantified using the Qubit Protein Assay Kit and a Qubit Fluorometer (Thermo Fisher). Sodium dodecyl sulfate–polyacrylamide gel electrophoresis and Western blotting were performed using pre-cast NuPAGE Bis-Tris 4 - 12% mini-gels (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... 20 mg total protein in 200 ml NLB was precleared with 10 ml protein A Dynabeads (Thermo Fisher Scientific) for 20 min at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... Protein content in cell lysates and EVs was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Between 5 and 20 μg of total protein (kept consistent for individual experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 μg of total protein were loaded on NovexTM 10% Zymogram Plus (Gelatin) Protein Gels (Thermo Fisher Scientific: ZY00102BOX) using 2x non-reducing sample buffer (1M Tris-HCl pH 6,8 (Carl Roth ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration in the samples was quantified with the Pierce™ BCA Protein Assay Kit (23227, Thermo Scientific) and equal amount of proteins were loaded on the gel ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated in an NuPAGE™ Novex™ 4-12% Bis-Tris protein gel (WG1402BOX, Thermo Fisher Scientific) in NuPAGE™ MOPS SDS running buffer (50 mM MOPS ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentrations in the lysates were measured with the Pierce BCA Protein Assay Kit (Thermo Scientific™, CAT: 23227). Lysate mixed (10-25 μg ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein was measured using Pierce BCA Protein Assay Kit according to the manufacture’s microplate protocol (Thermo Fisher 23225). 1X Laemmli sample buffer (Bio-Rad 161-0747 ...
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bioRxiv - Genomics 2022Quote: ... The protein concentration of supernatant lysates was determined using the Pierce 660 nm Protein Assay Kit (Thermo Fisher Scientific) and a NanoDrop ND1000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2023Quote: Protein samples were prepared from 10-20 males and females in protein extraction buffer plus 1% protease inhibitor (ThermoFisher) and 1% phosphatase inhibitor (Sigma ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Concentrations of solubilized protein precipitates were determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Bremen, Germany). For LC-MS/MS ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected and the protein concentration was quantified using bicinchoninic acid protein assay (BCA assay; Pierce, Thermo Scientific). 50 μg of protein lysate was denatured and were run on a 4-12% SDS-PAGE gel ...
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bioRxiv - Cell Biology 2022Quote: ... The total protein concentration was determined using the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher #53226). 30 μg of total protein sample was mixed with Laemmli buffer (180 mM DTT (Sigma #D9779) ...
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bioRxiv - Genomics 2023Quote: ... Protein quantification for each fraction was done using manufacturer’s protocol for Pierce 660nm protein assay reagent (Thermo Scientific, USA). Each Fraction was subjected to iCLIP protocol as described in Huppertz et al 201441 using rabbit-CLAMP antibody to immuniprecipitate bound RNAs which were extracted using proteinase K and phenol:chloroform ...
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bioRxiv - Biochemistry 2023Quote: ... Protein bands were visualised by Coomassie blue staining using QuickBlue Protein Stain (LuBio, Zürich, Switzerland) and accompanied by either a SeeBlueTM Plus2 Pre-stained Protein Ladder (Invitrogen) or a PageRuler Prestained Protein Ladder (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were clarified and proteins were immunoprecipitated using α-FLAG M2 magnetic beads or protein G-conjugated Dynabeads (ThermoFisher) pre-incubated with rabbit α-GFP antibody (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... The EV and cell lysate protein concentrations were determined using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). The protein lysates were separated using Bolt 4–12% Bis-Tris Plus Gels (Burlington ...
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bioRxiv - Synthetic Biology 2022Quote: ... The content of protein in the samples was determined with the A280 Total Protein Assay (Thermo Scientific, Wilmington, DE). A volume equivalent to 100 μg protein per sample was taken through a modified filter-aided sample preparation (FASP ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentrations of the EV isolates were determined using the PierceTM BCA protein assay kit (Thermo Fisher Scientific) and 10 ug aliquots were prepared and stored at -80°C until further analysis.
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration of the supernatant was then determined using Pierce™ BCA Protein Assay Kit (# PI23225, Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Protein concentrations of all samples were determined using the Pierce Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... Results were normalized by total protein concentrations as determined by Pierce BCA Protein Assay Kit (ThermoFisher Scientific, catalog #23225). PBMC lysates were not diluted for this assay ...
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bioRxiv - Cancer Biology 2023Quote: Bicinchoninic acid (BCA) assay protein concentration determination: The Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, cat no. 23225) was used for protein quantification ...
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bioRxiv - Plant Biology 2023Quote: ... Total protein content in the supernatant was estimated using the Pierce BCA protein assay kit (Thermo Scientific, Rockford, IL). The protein samples were denatured at 65 °C in the 1X LDS sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: Lysates containing 25 µg of protein were electrophoresed on a NuPage 4%-12% Bis-Tris Protein gel (Thermofisher Scientific). Proteins were transferred to a PVDF membrane (0.2 µm ...
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bioRxiv - Physiology 2023Quote: ... Protein concentrations were determined with a BCA assay (Pierce™ BCA Protein Assay Kit; #23225, ThermoFisher, Carlsbad, CA, USA). Total protein (5∼15 µg ...
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bioRxiv - Biochemistry 2023Quote: ... Purified and concentrated EGFP_Nanobody protein was immobilized onto agarose beads (20 mg protein per column) using AminoLink Plus Immobilization Kit (ThermoFisher), following manufacturer’s instructions in PBS pH 7.5 coupling buffer.
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bioRxiv - Cancer Biology 2023Quote: ... Protein extraction was performed lysing the cells with M-PER (Mammalian Protein Extraction Reagent, ThermoFisher Scientific, Waltham, MA, USA) lysis buffer plus Halt Protease and Phosphatase Inhibitor Cocktail ...
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bioRxiv - Plant Biology 2023Quote: ... and protein expression was induced by methanol in BMMY medium following the description in Pichia protein expression handbook (Invitrogen). SCP8 protein was purified by affinity chromatography with a His-trap column (Cytiva ...
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bioRxiv - Physiology 2023Quote: ... The respective protein concentration was measured using the PierceTM BCA Protein Assay Kit (Thermo Fisher Scientific; Waltham, MA, USA). Mitochondria-rich fraction (10 µg of protein ...
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bioRxiv - Biochemistry 2023Quote: ... The protein concentration of each AF sample was measured using a Pierce BCA Protein Assay kit (Thermo scientific 23227) and then normalized to equal concentrations for downstream analysis.
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bioRxiv - Cell Biology 2023Quote: ... and protein concentration of the supernatant was determined by Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific; 23225). Spastin protein was resolved on 8% SDS-PAGE gels transferred to Immobilon®-FL PVDF membranes (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... while the protein content was measured using the Pierce™ BCA Protein Assay Kit (catalog no: 23225, ThermoFisher Scientific), following manufacturers’ instructions.
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bioRxiv - Plant Biology 2023Quote: ... Protein abundance was determined using the protein area calculated by the Proteome Discoverer software (Thermo Fisher Scientific; version 1.4).
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bioRxiv - Molecular Biology 2023Quote: ... Equivalent protein quantities for all genotypes were loaded onto a Nupage 4-12% Bis-Tris Protein Gels (ThermoFisher Scientific) for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... Remaining protein pellets were dissolved with 8M urea and total protein was quantified using Pierce BCA assay (Thermo Fisher). Total protein amount was used for equivalent loading for High Performance Liquid Chromatography and High-Resolution Mass Spectrometry and Tandem Mass Spectrometry (HPLC-MS/MS ...
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bioRxiv - Immunology 2023Quote: ... Remaining protein pellets were dissolved with 8M urea and total protein was quantified using Pierce BCA assay (Thermo Fisher). Total protein amount was used for equivalent loading for High Performance Liquid Chromatography and High-Resolution Mass Spectrometry and Tandem Mass Spectrometry (HPLC-MS/MS ...
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bioRxiv - Systems Biology 2023Quote: ... Protein concentrations in the pellet were determined using a Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, MA). After mixing 400 μL of supernatant with 400 μL of chloroform and 320 μL of water ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were clarified by centrifugation at 4°C and the protein concentration was determined by BCA protein assay (Thermofisher). Samples were resolved on a precast 4%–12% gradient gel (Thermo Fisher Scientific ...