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Citations for Thermo Fisher :
9451 - 9500 of 10000+ citations for Mouse Anti Hepatitis C Virus Core Protein Antibody 1862 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Immune complexes were pulled down for 45 min at 4°C with 25 µL protein G dynabeads (Invitrogen), captured on a magnet and washed 4 times in 800 µL IP buffer ...
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bioRxiv - Cell Biology 2024Quote: LZTR1WT and LZTR1L580P were expressed as C-terminal His-tagged proteins in Expi-293F cells (Thermo Fisher Scientific). pcDNA3.1-LZTR1-Myc-6xHis plasmid was a gift from Jens Kroll (Heidelberg University and German Cancer Research Center (DKFZ-ZMBH Alliance)).56 The LZTR1L580Pvariant was introduced into the plasmid by site-directed mutagenesis as previously described.11 Cells were transfected using ExpiFectamine 293 Reagent (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... were pre-bound overnight at 4°C to 60 μl of Dynabeads protein G (10003D, Thermo Fisher Scientific) diluted in 250 μl of blocking buffer (PBS ...
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bioRxiv - Microbiology 2024Quote: ... 4 °C) and protein concentration in the supernatant was determined by a commercially available BCA assay (ThermoFisher Scientific). The same protein amount was used for each measurement.
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bioRxiv - Cell Biology 2024Quote: ... protein samples were denatured at 70°C for 10 min in LDS Sample Buffer (Thermo Fisher Scientific, NP0007) with or without 100 mM Dithiothreitol (+DTT and –DTT ...
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bioRxiv - Neuroscience 2022Quote: ... Protein samples were incubated at 100°C for 5 min with NuPAGE Sample loading dye (Thermo Fisher, NP0007) and dithiothreitol as reducing agent ...
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bioRxiv - Genomics 2023Quote: ... The chromatin was incubated overnight at 4°C with 50 μl Protein A magnetic beads (Dynabeads, Thermo Fisher) pre-bound with 2.5 μl α-CENH3 (gift of Prof ...
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bioRxiv - Cell Biology 2023Quote: ... 1:2 diluted whole cell extract in buffer C was added to Dynabeads Protein G (Thermo Fisher Scientific) pre-incubated with HEXIM1 antibody ...
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bioRxiv - Cell Biology 2023Quote: ... 4°C) and the protein concentration in cellular lysates was determined by absorbance on a nanodrop instrument (ThermoFisher). 1 mg of lysate in 1 mL of lysis buffer was incubated overnight at 4°C with 20 μL of anti-FLAG M2 magnetic bead slurry (MilliporeSigma ...
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bioRxiv - Cancer Biology 2024Quote: ... at 100°C and 15 μg of protein were separated with 4–12% Bis-Tris gels (Invitrogen, US) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Immunology 2024Quote: ... 5μg of protein were boiled for 2min at 85°C in NovexTM Tricine SDS Sample Buffer 2X (Invitrogen), separated on NovexTM 10%-20% Tricine Protein Gels (Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... and immunostained for 16 h at 4°C with primary antibodies followed by Alexa Fluor® 488- or 546-conjugated secondary antibodies (Molecular Probes) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies (Supplemental Table 1) were incubated at 4°C overnight and secondary antibodies (Alexa Fluo 488, 555, or 647; Thermo Fisher Scientific) were incubated at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies were incubated overnight at 4°C followed by washing and incubation with alexa-conjugated secondary antibodies (Invitrogen, 1:1000 dilution) at room temperature for 1-3 hour in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed to remove the excess of primary antibody and incubated with the appropriate Alexa-Fluor secondary antibodies for 2 h at 25 °C (1:1000, Thermo Fisher Scientific). The excess of secondary antibody was washed ...
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bioRxiv - Neuroscience 2021Quote: ... and then immunostained for 16 h at 4°C with primary antibodies followed by Alexa Fluor® 568- or 633-conjugated secondary antibodies (Molecular Probes) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Immunohistochemistry was performed by incubating with primary antibodies (overnight, 4°C) and fluorophore-conjugated secondary antibodies (2 hr, room temperature; ThermoFisher, Jackson ImmunoResearch). Sections were mounted with VectaShield (VECTOR ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour, followed by incubation with primary antibodies (overnight, 4°C) and secondary antibodies (1-2 hours, from Jackson ImmunoResearch or ThermoFisher Scientific). The slides were then mounted with coverslips using VECTASHIELD (Vector Labs ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were blocked with 4% horse serum in PBS for 1 hour and then incubated with primary antibodies overnight at 4 °C followed by incubation with appropriate fluorogenic secondary antibodies (Thermo Fisher Scientific) at room temperature for 1 hour.
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bioRxiv - Cell Biology 2020Quote: ... Immuno-complexes were visualized with Alexa Fluor 488-labeled goat anti-rabbit IgG antibody and Alexa Fluor 568-labeled goat anti-guinea pig IgG antibody (Invitrogen). Cells were observed by a confocal laser microscope (LSM710 with Axio Observer Z1 ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 2h at room temperature with a goat anti-rat antibody and/or a goat anti-rabbit antibody or Alexa-647 phalloidin (Invitrogen) if needed ...
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bioRxiv - Neuroscience 2021Quote: ... The secondary antibody used for anti-GFP staining was goat anti-rabbit Alexa Flour 488-conjugated antibody (A-11008; Thermofisher) used at 1:500 ...
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bioRxiv - Molecular Biology 2019Quote: ... and finally stained with the anti-sHP1γ antibody (1:250) and Alexa Fluor 488 anti-rabbit secondary antibody (1:500, Invitrogen). Coverslips were mounted in VectaShield mounting media with DAPI for immunofluorescence ...
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bioRxiv - Immunology 2021Quote: ... Virus-infected cells were detected using in-house raised rabbit anti-S polyclonal antibody along with peroxidase-conjugated goat anti-rabbit IgG (H+L) secondary antibody (Invitrogen). MN titers represented the reciprocal of the highest serum dilution that yielded >50% reduction in OD values as compared to wells containing virus only ...
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bioRxiv - Neuroscience 2020Quote: ... Biotinylated surface GLT-1 transporters were identified by using anti-GLT primary antibody (1:500, Alomone) or anti-V5 primary antibody (1:2000, Invitrogen) and detection of enhanced chemilluminescence from HRP-coupled anti-rabbit secondary antibodies followed by detection with an ImageQuant LAS4000 mini imaging system and analysis with ImageQuant software (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... The viral spike protein was stained with an in-house developed rabbit anti-spike antibody and goat anti-rabbit IgG (H+L) highly cross-adsorbed antibody with Alexa Fluor 488 (Invitrogen). Images were acquired using FluoView FV10i Confocal Laser Scanning Microscope (Olympus) ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were detected by secondary anti-rabbit antibody conjugated with Alexa Fluor 488 and anti-goat antibody conjugated with Alexa Fluor 555 (Invitrogen), resulting in tdTomato protein being visible as red fluorescence and CRF peptide visible as green fluorescence ...
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bioRxiv - Microbiology 2021Quote: ... the goat anti-ACE2 primary antibody (R&D) and Alexa Fluor 568 donkey anti-goat IgG (H+L) secondary antibodies (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and anti-VE-cadherin antibody (1:100, Cell Signalling) followed by anti-rabbit Alexa 488 secondary antibody (1:1000, Invitrogen) to mark EC junctions.
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated for 1 h with primary/detection SARS-CoV-2 anti-RBD rabbit polyclonal antibody (Sinobiologicals) and then 1 h with secondary anti-rabbit HRP-conjugate antibody (Invitrogen). Foci were visualised using TrueBlue™ Peroxidase Substrate (Sera Care ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody labelling was performed with primary anti-ACTN4 (rabbit; ENZO) and secondary anti-rabbit-AlexaFluor488 antibody (goat; Thermo Fisher Scientific). Nuclear compartment was labelled with DAPI ...
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bioRxiv - Neuroscience 2022Quote: ... cultures were incubated in rabbit anti-RIM 1/2 antibody (1:200) followed by donkey anti-rabbit AF647 antibody (1:500; Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... The membrane probed with an anti-transthyretin antibody was further incubated with goat anti-rabbit secondary antibody (1:1,000; Invitrogen 31460). The membrane was washed three times for ten minutes and then incubated with an enhanced chemiluminescence reagent (Promega W1001) ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated for 2h at room temperature with a goat anti-rat antibody and/or a goat anti-rabbit antibody or Alexa-488 phalloidin (Invitrogen) if needed ...
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bioRxiv - Cell Biology 2023Quote: Alexa 647 labelled Goat anti-Rabbit IgG secondary antibody and anti-alpha tubulin primary antibody was purchased from Thermo Fisher Scientific (Australia) ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated at 4°C with anti-Nrf2 antibody (thermo fisher scientific MA5-42371) and then an anti-rabbit alexa 488 antibody was used (Invitrogen). Nuclei were stained with DAPI ...
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bioRxiv - Physiology 2024Quote: ... and incubated overnight at 4°C in primary antibodies (1:500 anti-NALCN, Alomone labs; 1:4000 anti-SLO2.1, Absolute Antibody; 1:50 alpha-Smooth muscle actin, Invitrogen). Slides were incubated in biotinylated secondary antibodies in 10% GS for 30 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... chicken anti-GFP, Abeam, Cat# ab13970; 1:200, mouse anti-GFP, Roche, Cat# AB_390913; 1:1000, rabbit anti-GFP, Invitrogen, Cat# A-l 1122 ...
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bioRxiv - Neuroscience 2024Quote: ... Donkey anti-Mouse 568, Donkey anti-Rabbit 657, Donkey anti-Rat 488, Donkey anti-Goat 568; all at 1:1,000; Invitrogen) for 2 hours at room temperature ...
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Elimination of glutamatergic transmission from Hb9 interneurons does not impact treadmill locomotionbioRxiv - Neuroscience 2021Quote: ... Secondary Abs (Alexa 546 Donkey anti-Rabbit; Alexa 488 Donkey anti-goat, anti-mouse, or anti-sheep, all 1:400, Invitrogen, Oregon, USA) were diluted in a solution containing 1% donkey serum and PBST ...
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bioRxiv - Immunology 2021Quote: ... or with unconjugated 4.3.1.3 mouse MAb and secondary detection with goat anti-mouse Alexa-Fluor 647 (A-21235; Life Technologies). Immunofluorescence was used to boost GFP signal in TgBAC(lck:gfp)vcc6 specimens with a chicken anti-GFP primary (Abcam ...
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bioRxiv - Immunology 2024Quote: CLP was performed on WT and Plk3−/− mice following retro orbital injection (0.1μg/g mouse) of anti-mouse GPIX (Emfret) conjugated to AlexaFluor-750 (ThermoFisher) as previously described 21 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-lamin A/C phosphorylated at S22 (PA5-17113 from Invitrogen/Thermo Fisher Scientific or D2B2E from Cell Signaling ...
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Neutralizing PD-L1 and PD-L2 Enhances the Efficacy of Immune Checkpoint Inhibitors in Ovarian CancerbioRxiv - Cancer Biology 2020Quote: ... and resuspended in PBSA with 1:250 of chicken anti-c-Myc (Invitrogen) for 1 h at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... 1:150 overnight at 4 °C followed by anti-rabbit 594 (Invitrogen A11012) 1:400 for 1h at room temp ...
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bioRxiv - Molecular Biology 2019Quote: ... 50 μl of Pierce anti-c-Myc magnetic beads (88843, Thermo Fisher Scientific) was used for each sample.
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bioRxiv - Biochemistry 2020Quote: ... Anti-His (C-term)-HRP (#46-0707, 1:2000 for dot blot, Invitrogen); Aβ (6E10 ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated overnight at 4 °C with chicken anti-GFP (ThermoFisher). Then sections were washed in PBST 4 times for 10 min ...
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bioRxiv - Immunology 2023Quote: ... 50 µg/ml anti-HLA-A,B,C (W6/32; Invitrogen, #MA1-19027) were added ...
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bioRxiv - Neuroscience 2023Quote: ... at 4 °C overnight or rabbit anti-HA (Invitrogen #MA5-27915, 1:1000) at 4°C over two nights ...