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Citations for Thermo Fisher :
901 - 950 of 3033 citations for IFN gamma Rat CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... anti-rat AlexaFluor 555 (ThermoFisher, A48270, RRID: AB_2896336), anti-goat AlexaFluor 488 (A11055 ...
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bioRxiv - Cell Biology 2024Quote: ... anti- rat Alexa Fluor 594 (Invitrogen; A-11007),
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bioRxiv - Cell Biology 2024Quote: ... donkey anti-rat Alexa Fluor 647 (Molecular Probes). Nanobody (Atto 542 FluoTag-X2 anti-Mouse Kappa light chain ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-rat-HRP (1:10000, #31470 Fisher Scientific) and anti-goat-HRP (1:10000 ...
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bioRxiv - Cancer Biology 2024Quote: ... and goat anti-rat AF647 (Invitrogen; A-21247), then washed 3 times with PBS and mounted using Fluoromount ...
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bioRxiv - Immunology 2022Quote: ... Murine IFNγ levels and IL-2 levels were measured using the Mouse Interferon gamma (IFNγ) Uncoated ELISA Kit (Thermo Fisher Scientific, 88-7314-76) or IL-2 Mouse Uncoated ELISA Kit (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... All microplate reader measurements were normalised to total protein content per sample using the Pierce™ BCA Protein Assay and Bovine Gamma Globulin assay (Thermo scientific, Loughborough, UK).
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bioRxiv - Developmental Biology 2019Quote: ... The silicon device surfaces were functionalized with a previously described protocol using 3-mercaptopropyltrimethoxysilane (MPTMS) and N-gamma-maleimidobutyryloxysuccinimide ester (GMBS) NeutrAvidin-biotin chemistry (Thermo Fisher Scientific, Rockford, IL)[32 ...
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bioRxiv - Immunology 2022Quote: ... Monoclonal antibodies specific for IFN-γ (12-7319-42) and granzyme B (12-8896-42) were purchased from Thermofisher. Durvalumab (anti-PD-L1 antibody ...
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bioRxiv - Immunology 2020Quote: The IFN-γ concentration in the sera was analyzed by standard sandwich ELISA kit according to manufacturer’s instructions (ThermoFisher).
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bioRxiv - Microbiology 2020Quote: ... In particular IFN-γ and TNF-α were evaluated by Mouse Custom ProcartaPlex 9-plex (Invitrogen, Thermo Fisher Scientific) and normalized at 2500 ug/ml of quantified proteins in lung supernatants.
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bioRxiv - Microbiology 2020Quote: ... In particular IFN-γ and TNF-α were evaluated by Mouse Custom ProcartaPlex 9-plex (Invitrogen, Thermo Fisher Scientific) and normalized at 2500 ug/ml of quantified proteins in lung supernatants.
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bioRxiv - Bioengineering 2019Quote: ... and stained with IL2-XE114-TNFmut (final concentration 10μg/mL) and detected with rat anti-IL2 (eBioscience 14-7029-85) followed by staining with anti-rat AlexaFluor488 (Invitrogen A21208). IL2-KSF-TNFmut (specific for an irrelevant antigen ...
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bioRxiv - Cell Biology 2019Quote: ... 3 rat anti-Rab1 and 3 rat anti-Rab11 (1:2000 concentrated supernatant) as primary antibodies and HRP-conjugated anti-rat IgG antibodies (1:20000, Life Technologies) as secondary antibodies ...
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Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: ... single cells were re-suspended at a density of 1-2 × 107 and subjected to magnetic sorting to exclude immune cells using rat-anti-mouse CD45 (R&D, MAB114) and CD24 (eBioscience, clone M1/69) magnetic sheep-anti-rat Ig Dynabeads (Invitrogen, #11035). FACS for FITC+/RFP-/DAPI-was used to obtain GFP+ve CAFs and to exclude RFP+ve tumour cells and dead cells ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with unconjugated lineage rat antibodies (CD3, CD4, CD5, CD8, B220, Gr1, Mac1, and Ter119) followed by goat-α-rat PE-Cy5 (Invitrogen). Stem and progenitor cells were isolated using fluorescently labeled or biotinylated antibodies for the following antigens ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... M-100126-01-0005), rat Esrrg (Dharmacon, M-100212-01-0005), rat E2f1 (Dharmacon, M-102754-00-0005) or scramble control siRNA (ThermoFisher, 4390843), using the Viromer BLUE Transfection Reagent (Lipocalyx ...
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bioRxiv - Neuroscience 2022Quote: ... The expression of Kir2.1EGFP was assessed with anti-GFP antibody (rat monoclonal Ab from Nacalai, Japan, GF090R; the secondary antibody, 488-anti Rat IgG from Invitrogen, A11006) (Mizuno et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... bakeri worm lysates or EVs were carried out with rat polyclonal anti-exWAGO antibody (raised against full length protein) or rat IgG (control) using protein L magnetic beads (Fisher Scientific). Equivalent amounts of the input ...
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bioRxiv - Neuroscience 2024Quote: ... To visualize Gi-DREADD-mCherry expression in rCA2 of injected mice sections were stained with 1⁰ rat anti-mCherry (Life Technology #M11217) 1:500 + 2⁰ anti-rat 568 (Invitrogen #A11077) at 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... In order to visualize AAV-retro positive cells sections were stained for mCherry (1⁰ rat anti-mCherry (Life Technology #M11217) 1:500 + 2⁰ anti-rat 568 (Invitrogen #A11077) at 1:250 ...
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bioRxiv - Cell Biology 2022Quote: Chinese Hamster Ovary (CHO) cells (ATCC® CCl-61™) were maintained at 37°C and 5% CO2 in DMEM/F-12 (Thermo Fisher, Cat# 11330057) with 10% FBS (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... The immunoglobulin kappa-only and dual-gene (immunoglobulin kappa and immunoglobulin gamma) plasmids were transfected at a 2:1 molar ratio into ExpiCHO cells using ExpiFectamine (Thermo Fisher Scientific, Waltham, MA, USA). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... and mACP-HA2 was visualized with the rat anti-HA 3F10 primary antibody and FITC-conjugated donkey anti-rat 2° antibody (Invitrogen Life Technologies A18746). Images were taken on an EVOS M5000 imaging system or Zeiss 880 laser-scanning confocal microscope fitted with an Airyscan detector ...
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bioRxiv - Microbiology 2023Quote: ... and the endogenously C-terminal HA-tagged cytochromes were visualized with a Roche Rat anti-HA monoclonal 3F10 primary antibody and FITC-conjugated Donkey anti-Rat 2° antibody (Invitrogen Life Technologies A18746). Images were taken on DIC/brightfield ...
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bioRxiv - Microbiology 2023Quote: ... Lysates and immunecomplexes were separated by SDS-PAGE and blotted to detect either biotynlated proteins (IRDye® 680LT streptavidin (Li-Cor)) or CDPK3HA (rat-anti-HA)/anti-rat DyLight™ 800 (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... Alexa488 anti-rat and Cy3 anti-rat (1:250, Jackson) secondary antibodies or with Alexa647-conjugated Streptavidin (1:200, Thermo Fisher Scientific) and by counterstaining with DAPI (0.2 µg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... sera samples and conditioned tissue culture medium from rat hepatic stellate cells (HSCs) or primary rat hepatocytes (PCs) were stained with phycoerythrin (PE)-labeled Cell Mask (Thermo Fisher Scientific) or stained with Syto RNASelect (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2023Quote: ... appropriate secondary antibodies were treated for 1-2 hours in the dark (AF555 anti-Rabbit, AF555 anti-Rat, AF647 anti-Rat (Invitrogen, Waltham, MA)) and washed in PBS containing 0.01% Triton X-100 ...
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bioRxiv - Immunology 2023Quote: ... the appropriate secondary antibodies were added diluted in 1% BSA in PBS for 1 hour at a 1:1000 dilution (Goat anti Rat 488 Thermo Fisher Scientific a11006, Goat anti Rat 647 Thermo Fisher Scientific a21247 ...
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bioRxiv - Biochemistry 2020Quote: ... AlexaFluor 594–conjugated anti-rat (1:250, Molecular Probes) and AlexaFluor 488–conjugated anti-mouse (1:250 ...
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bioRxiv - Cancer Biology 2021Quote: ... and goat α-rat (62-6520, Invitrogen, 1:5000). Proteins were detected using West Pico Chemiluminescent Substrate for 20 seconds (34578 ...
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bioRxiv - Cell Biology 2020Quote: ... and goat-α-rat 488 (Thermo Fisher A-11006) at 1:3,000 dilution ...
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bioRxiv - Cell Biology 2020Quote: Donkey anti rat Alexa Fluor 594 (A21209, Life Technologies) and donkey anti mouse Alexa Fluor 647 (A31571 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-rat Alexa Fluor 488/680 (Invitrogen, CA, USA), anti-mouse Alexa Fluor 488/800 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Dynabeads Sheep anti-rat igG was obtained from Invitrogen, life technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... 1:1000 secondary antibody (Goat anti-Rat 488 [Invitrogen, A11006] ...
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bioRxiv - Molecular Biology 2021Quote: ... and anti-rat Alexa Fluor 546 (1:500, Invitrogen). The incubations were performed in the dark in a humidified chamber ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-rat (goat polyclonal, Thermo Fisher, A-21434, RRID:AB_2535855); Alexa Fluor 594 ...
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bioRxiv - Immunology 2022Quote: ... rat IgG2a kappa isotype control (1:400, Thermo Scientific), armenian hamster IgG isotype control (1:400 ...
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bioRxiv - Immunology 2022Quote: ... rat IgG2a isotype control (1:400, Thermo Fisher Scientific), rat IgG2b kappa Isotype control (1:400 ...
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bioRxiv - Neuroscience 2022Quote: ... AF-647 goat anti-rat (Invitrogen #A21247; 1:500), Fluorescein (FITC)-conjugated donkey anti-rabbit (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2022Quote: ... goat anti-rat-Alexa375 1:300 (Thermo Fisher Scientific), rabbit anti-mouse-Cy3 1:400 (Dianova) ...
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bioRxiv - Neuroscience 2021Quote: ... and monoclonal rat anti-mCherry (#M11217, ThermoFisher, RRID: AB_2536611) primary antibodies in 0.1M Tris-buffered saline containing 0.5% Roche Blocking Reagent (#11096176001 ...
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bioRxiv - Neuroscience 2021Quote: ... Goat anti-Rat A-546 (1:500, Invitrogen, #A11081), Goat anti-Rabbit A488 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-rat 647 (1:400, Invitrogen #A-21247), donkey anti-chicken 405 (1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... goat anti-rat IgG (1:5000, Thermo scientific, 31470), red fluorescence donkey anti-mouse antibody Alexa Flour® 647 (1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... Goat polyclonal anti-Rat HRP (Invitrogen, 31470, 1:1,000).
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bioRxiv - Molecular Biology 2019Quote: ... Alexa-555 Goat anti-Rat (ThermoFisher A21434, 1:750), Alexa-647 Donkey anti-Sheep (ThermoFisher A21448 ...
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bioRxiv - Cell Biology 2022Quote: ... and anti-rat 647 (1:500, Thermo Fisher Scientific), were used for visualization ...