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Citations for Thermo Fisher :
701 - 750 of 3033 citations for IFN gamma Rat CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... don-key anti-rat 488 (Invitrogen A21208).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... a Rat bFGF ELISA Kit (Invitrogen, CA), and an AssayMax™ Dihydrotestosterone ELISA Kit (Assaypro ...
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bioRxiv - Neuroscience 2023Quote: ... goat-anti rat (1:4000; 31470, Invitrogen), and goat-anti rabbit (1:4000 ...
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bioRxiv - Microbiology 2023Quote: ... anti-rat AF647 (Invitrogen, A21247, 1:500), anti-chicken AF546 (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... collagen (Collagen I, Rat Tail, Gibco A1048301), or laminin (Roche 11243217001 ...
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bioRxiv - Neuroscience 2023Quote: ... goat anti-rat IgG (1:1,000; Invitrogen), Alexa Fluor 488-conjugated goat anti-rabbit IgG or goat anti-mouse IgG (1:300 ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa Fluor 488 anti–rat (A11006; Invitrogen), Alexa Fluor 594 Phalloidin (A12381 ...
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bioRxiv - Cell Biology 2023Quote: Type 1 rat-tail collagen (Life Technologies) was used at a stock concentration of 3 or 10 mg mL-1 ...
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bioRxiv - Neuroscience 2024Quote: ... and Rat Alexa fluor 647 (Thermo Fisher Scientific Cat# A21247 ...
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bioRxiv - Neuroscience 2024Quote: ... Donkey anti-Rat 647 IgG (#A21247, ThermoFisher).
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bioRxiv - Neuroscience 2024Quote: ... Donkey anti-Rat 488 IgG (#A21208, ThermoFisher), Donkey anti-Rat 555 IgG (#A21434 ...
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bioRxiv - Neuroscience 2024Quote: ... Donkey anti-Rat 555 IgG (#A21434, ThermoFisher), Donkey anti-Rat 647 IgG (#A21247 ...
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bioRxiv - Cancer Biology 2024Quote: ... Goat anti-Rat IgG-AF647 (Invitrogen, A21247) and AF488-goat anti-mouse (A11017 ...
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bioRxiv - Neuroscience 2024Quote: ... and rat cortical astrocytes (Gibco™, N7745100) were seeded at a density of 100 cells/mm2 ...
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bioRxiv - Cell Biology 2024Quote: ... rat anti-alpha tubulin (MA1-80017, Invitrogen), and human anti-centromere protein antibody (Antibodies Incorporated) ...
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bioRxiv - Developmental Biology 2022Quote: ... Rat anti-E-cadherin 1:200 (DCAD2, DSHB) and donkey anti-rat Alexa 488 1:200 (Thermo Fisher Scientific) antibodies were used ...
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bioRxiv - Immunology 2022Quote: ... the cells were stained with unconjugated rat anti-Ly49A antibody (YE1/32.8.5, Stemcell) followed by AF555 conjugated goat-anti-rat IgG (Life Technologies), both were done for 45 minutes in PBS at RT ...
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bioRxiv - Microbiology 2023Quote: ... followed by a secondary anti-rat IgG antibody conjugated with Alexa Fluor 568 (anti-rat-AF568) (Thermo Fisher Scientific). Fluorescent images were captured with the CQ1 Confocal Quantitative Image Cytometer (Yokogawa Electric Corporation ...
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bioRxiv - Developmental Biology 2022Quote: ... Rat TS cells were maintained in Rat TS Cell Stem State Medium (RPMI-1640 culture medium (11875093, Thermo Fisher) containing 20% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected using JetPEI® DNA transfection reagent (CHO dhFr- cells, Polyplus Transfection, Illkirch-Graffenstaden, France) or lipofectamin®2000 reagent (COS-7 and MDCK cells, Invitrogen, Carlsbad, US). Experiments were performed 24h (TIRF imaging ...
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bioRxiv - Immunology 2021Quote: ... the transfected cells were transferred to T-75 flasks at a 2 × 106 vc/mL density for a 1-day recovery in EX-CELL CD CHO Fusion medium (MilliporeSigma, Burlington, MA, USA) with 6mM GlutaMAX (Gibco; Waltham, MA, USA). After 1-day recovery ...
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bioRxiv - Immunology 2021Quote: Sigma Aldrich’s CHOZN cells were cultured in EX-CELL CD CHO Fusion medium (MilliporeSigma, Burlington, MA, USA) supplemented with 6 mM GlutaMAX (Gibco, Waltham, MA, USA), shaking at 37°C ...
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bioRxiv - Biochemistry 2022Quote: For confocal microscopy 25,000 adherent CHO-K1 cells were seeded on 8-well Nunc™ Lab-Tek™ (Thermo Scientific™, United States) and incubated overnight ...
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bioRxiv - Cell Biology 2022Quote: ... The PACRG/pEGFP-N2 and DNALI1/Flag were transfected individually or together into CHO cells by using Lipofectamine™ 2000 transfection reagent (Invitrogen, Waltham, MA). The CHO cells were cultured with DMEM (with 10% fetal bovine serum ...
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bioRxiv - Immunology 2020Quote: ... of Chinese hamster ovary (CHO) cell cultures using the Gibco™ ExpiCHO™ Expression System and a protocol for deep 96-well blocks (ThermoFisher Scientific). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Chinese hamster ovary cells (CHO) (RRID: CVCL_0214) were grown at pH 7.4 in filtered sterile F12 (Ham’s) nutrient medium (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 5% FBS and maintained in a humidified environment at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2020Quote: CHO-K1 and the GAG-deficient variant CHO-745 cells (generous gift from Dr. Antonio Alcami, Spanish Research Council, Spain) were grown in DMEM/F12-Glutamax (Life Technologies, Carlsbad, CA) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... The CHO-K1 cells were grown in low glucose (1.0 g/L) Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific, Cat. no. 11,885-084) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: Total RNA extraction and isolation from normal and PSC liver cells or 3D-CHOs were performed using Trizol™ reagent and a PureLink RNA kit (Invitrogen, Waltham, MA). cDNAs were synthesized with a SuperScript™ First Strand Synthesis System (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: Chinese hamster ovary (CHO) cells transfected with CXCR3A were cultured in Ham’s F-12 growth medium (Gibco; Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... Flp-In CHO cells were chemically co-transfected with the linearized rDNA and circular pOG44 Flp-recombinase vector using Lipofectamine 3000 (Thermo Fisher Scientific, USA) following their protocol ...
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bioRxiv - Biochemistry 2023Quote: ... of paired heavy and light chain antibody sequences cloned into a single expression vector and expressed in Chinese hamster ovary (CHO) cells using the Gibco™ ExpiCHO™ Expression System and a protocol for deep 96-well plates (ThermoFisher Scientific). Briefly ...
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bioRxiv - Physiology 2019Quote: ... the AGE product was dialyzed against sterile PBS for 24 hours at 4°C using gamma-irradiated 10kDa cut-off cassettes (Thermo Scientific, Waltham, MA) to remove unreacted glycolaldehyde ...
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bioRxiv - Immunology 2021Quote: ... coated with anti-mouse IFN-γ capture antibody (clone AN18; Thermo Fisher Scientific; 8 μg/mL in PBS) and incubated overnight at 4°C ...
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Bat influenza vectored NS1-truncated live vaccine protects pigs against heterologous virus challengebioRxiv - Microbiology 2020Quote: ... plates were washed and incubated with 50 μl of 0.5 μg/ml biotinylated-porcine IFN-γ antibody (Invitrogen) for 2h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... were used before incubating splenocytes with anti-IFN-γ (No. 17-7311; eBioscience/Affymetrix, Santa Clara, CA, USA), anti-TNF-α (No ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-human TNF-α (hTNF-α)-PE and anti-human IFN-ɣ (hIFN-ɣ)-PECy7 were from ThermoFisher. Anti-human CD4 (hCD4)-BV711 was from Biolegend.
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bioRxiv - Microbiology 2022Quote: ... supernatants were collected for cytokine analysis by ELISA (IL-2 BD-OptEIA™; IL-17A, IFN-γ ThermoFisher) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... M0 cells were polarized into M1 with the treatment of 20ng/ml IFN-γ (RIFNG100, Invitrogen, United States), 20ng/ml IL-6 (RP8619 ...
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bioRxiv - Bioengineering 2023Quote: ... the medium was replaced in each well with complete medium containing 10 ng/mL IFN-γ (Life Technologies). After an additional 24 hours ...
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bioRxiv - Bioengineering 2019Quote: ... and cryostat section (10μm) were stained using the following antibodies: rat anti-IL2 (eBioscience 14-7029-85) and anti-rat AlexaFluor488 (Invitrogen A21208). For vascular staining goat anti-CD31 (R&D AF3628 ...
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bioRxiv - Bioengineering 2019Quote: Antigen expression was confirmed on ice-cold acetone fixed 8-μm cryostat sections of SKRC52 and CT26-CAIX stained with IL2-XE114-TNFmut and IL2-F8-TNFmut (final concentration 5μg/mL) and detected with rat anti-IL2 (eBioscience 14-7029-85) and anti-rat AlexaFluor488 (Invitrogen A21208). For vascular staining goat anti-CD31 (R&D AF3628 ...
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bioRxiv - Biophysics 2020Quote: Primary rat cortical neurons (RCN) prepared from the cortex of day-18 rat embryos (A10840-01, Life Technologies, Gaithersburg, MD) were suspended in a culture medium (21103-049 ...
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bioRxiv - Neuroscience 2022Quote: ... Serum TNF and IL-1β concentrations were determined by using a rat TNF alpha Uncoated ELISA and a rat IL-1β ELISA Kit (all from Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Macrophages were positively enriched from spleen single-cell suspensions using anti-F4/80 antibody (rat, Bioscience, Clone T45-2342)-coated Dynabeads (anti-rat IgG, Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Cells were then washed and incubated with anti-rat Ab magnetic beads at 1 bead/target cell for 40 min at 4C (Dynabeads sheep anti-rat IgG, Invitrogen). Cell suspensions were stained with Abs specific for congenic markers ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed and incubated with anti-rat Ab magnetic beads at 1 bead/target cell for 40 min at 4C (Dynabeads sheep anti-rat IgG, Invitrogen). Cells were sorted into 3mL of complete media (RPMI 1640 ...
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bioRxiv - Developmental Biology 2023Quote: ... using rat-anti-BrdU (1:500, OBT0030 Accurate) and donkey-anti-rat Alexa Fluor-568-conjugated secondary antibody (1:1000, Invitrogen).
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bioRxiv - Developmental Biology 2021Quote: ... and goat anti-rat 568 (Invitrogen, cat #A11077) antibodies in a dark humidified chamber for one hour at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... and anti-rat conjugated to Alexa647 (Thermofisher, A21472). Alexa568 conjugated phalloidin (Invitrogen ...