Labshake search
Citations for Thermo Fisher :
901 - 950 of 10000+ citations for 7 Bromo 5 fluoro 3 methyl 1H indole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... HT-7 (Thermo Scientific™, # MN1000), Tau pS202/T205 (AT8 ...
-
bioRxiv - Cell Biology 2020Quote: ... Harvested equine explants were allowed to recover in 6-well plates for 3-7 days in the following control medium: DMEM/F12 medium (11330, Thermo Fisher Scientific, Waltham, MA), 10% fetal bovine serum (Seradigm 1500-500 ...
-
bioRxiv - Immunology 2019Quote: ... was added to the cells with complete media for 30 min in 37°C and the caspase-3/7 positive cells were measured on an Attune NXT flow cytometer (Life Technologies, Carlsbad, Calif, USA). For TFH cell sorting ...
-
bioRxiv - Cell Biology 2019Quote: 1.5 cm-thick intact human brain slice (Number 7, see Fig. S3) was randomly chosen and passively incubated with 400 ml TO-PRO-3 (T3605, Thermo Fisher, 1:2000 dilution) in PBS at room temperature for 1 week ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were treated with aptamers at reported concentrations or with vehicle (DPBS and Mg2+) for 48 h and then stained according to the CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit (ThermoFisher Scientific, cat: #C10427). Briefly ...
-
bioRxiv - Immunology 2021Quote: ... Antibody was harvested from the cell supernatant after 5 to 7 days post inoculation and purified using Protein L Sepharose beads (Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2021Quote: ... Cells (1 × 107) from passage 5-7 were used for transplantation after staining with carboxyfluorescein succinimidyl ester (CSFE) (Invitrogen, Carlsbad, California, USA) on the day of the transplantation ...
-
bioRxiv - Neuroscience 2020Quote: We dissected brains and ventral nerve cords of 5-7 days old males in Schneider’s insect medium (Thermo Fisher Scientific, Waltham, MA) and fixed in 4% paraformaldehyde (PFA ...
-
bioRxiv - Cancer Biology 2021Quote: ... For the analysis of STAT3/5 phosphorylation cells were also labeled with phycoerythrin (PE)-cyanine 7 (Cy7)-conjugated anti-phospho-STAT3 (Tyr705) (Thermo Fisher Scientific) (#25-9033-42 ...
-
bioRxiv - Immunology 2022Quote: The production of NO was evaluated using 4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate (DAF-FM DA)(D23844; Invitrogen, Waltham, MA). Following leukocyte isolation ...
-
bioRxiv - Immunology 2020Quote: ... or aged (26-28 mo) female C57BL/6 mice and cultured for 7 days at 37°C and 5% CO2 in DMEM (Gibco, 11995-073) supplemented with 10% FBS ...
-
bioRxiv - Biochemistry 2020Quote: ... QPCR was performed on standards of diluted genomic DNA and cDNA products using specific primers (Supplemental table 5) on a ViiA 7 Real-Time PCR system (Thermo Fisher Scientific). Galnts gene copy numbers were calculated using the genomic DNA as a standard curve and variation between biological replicate was normalized using Actb expression level.
-
bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
-
Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: Bone marrow was collected from tibias and femurs of 4-6 mo female C57BL/6 mice and cultured for 7 days at 37°C and 5% CO2 in DMEM (Gibco,11995-073) supplemented with 10% FBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... 95°C for 20sec then 40 cycles of 95°C for 3sec and 58°C for 45sec on the QuantStudio 5 and 7 qRT-PCR systems (Applied Biosystems, CA). A known negative sample and non-template control (NTC ...
-
bioRxiv - Cell Biology 2022Quote: Fast-growing human embryonic kidney 293T cells (HEK293FT) were maintained in DMEM 10% FBS (v/v) and passaged at 5-7 day intervals with TrypleExpress (Invitrogen GIBCO, Paisley, UK,) to detach the cells ...
-
bioRxiv - Cell Biology 2022Quote: ... 2’,7’-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated goat anti-mouse and rabbit IgG antibodies were purchased from Jackson ImmunoResearch (West Grove ...
-
bioRxiv - Developmental Biology 2024Quote: Ovaries from 5-7 females were dissected in 1% PBS-Tween and fixed in a 1:1 mixture of 4% formaldehyde (Thermo Scientific, 28908) and heptane (Sigma Aldrich ...
-
bioRxiv - Physiology 2024Quote: ... The starvation assay consisted of placing 5-7 day-old female DTKR-RNAi flies and control flies into vials containing 1% aqueous agar (Fisher Scientific, USA) [48,73].
-
bioRxiv - Cancer Biology 2022Quote: ... for 1h at room temperature and mounted in ProLong Diamond Reagent with DAPI (Molecular Probes). Images were captured on a Zeiss LSM880 confocal microscope and analyzed with Fiji freeware.
-
bioRxiv - Genomics 2019Quote: ... for 1h at 37°C and Proteinase K Solution (20 mg/mL, Thermo Fisher Scientific) for 1h at 55°C ...
-
bioRxiv - Cancer Biology 2021Quote: ... imatinib or sunitinib for 1h or 8h were labelled with 25 µM HPG (Invitrogen, C10102) for the last 30 min of the drug treatment in L-methionine-depleted medium containing 5% dialyzed fetal calf serum (FCS ...
-
bioRxiv - Cancer Biology 2020Quote: ... were incubated for 1h at room temperature and proteins visualized by chemiluminescence (Thermo Fisher Scientific). Each experiment was repeated at least four times.
-
bioRxiv - Cell Biology 2020Quote: ... incubated 1h at room temperature with Alexa Fluor-conjugated secondary antibodies at 1:1,000 (ThermoFisher), washed 3 times in PBS and counterstained with DAPI at 1 μg.mL−1 in PBS before mounting.
-
bioRxiv - Cell Biology 2020Quote: ... EDL muscles were dissected from tendon-to-tendon and incubated for 1h in DMEM (Gibco) containing 0.25% collagenase I (Worthington) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor 594-conjugated secondary antibodies was added for 1h at room temperature (Molecular Probes), and nuclei were counterstained using SlowFade Gold Antifade reagent (Vector ...
-
bioRxiv - Microbiology 2021Quote: ... Supernatants were precleared 1h at room temperature with magnetic beads blocked with BSA (ThermoFisher Scientific) to avoid aspecific binding ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 10 % (vol/vol) Fetal Bovine Serum (Gibco; Heat-inactivated for 1h at 70°C). Stable cell lines were obtained by transfection with pMT puro vectors containing either Zasp52-PF or Zasp52-PF-ABM using Effectene (Qiagen ...
-
bioRxiv - Microbiology 2023Quote: ... for 1h and revealed using a 1/300 dilution of FITC-conjugated streptavidin (Invitrogen; SNN1008). Staining of DNA was performed on fixed cells by incubating them for 5 min in a 1 μg/ml 4,6-diamidino-2-phenylindole (DAPI ...
-
bioRxiv - Cell Biology 2023Quote: ... during 1h at room temperature before mounting in ProLong Gold Antifade reagent (P36941, Life Technologies). F-Actin was stained using Actistain (1:150 ...
-
bioRxiv - Cell Biology 2023Quote: ... for 1h at 37°C and then with Proteinase K (Fisher Scientific, #25-530-049) for 2h at 55°C ...
-
bioRxiv - Neuroscience 2023Quote: ... they were probed for 1h at RT with the fluorescent secondary antibodies (1:1000: ThermoFisher). Cell nuclei were stained with Hoechst 33342 (10 μg/mL ...
-
bioRxiv - Genetics 2023Quote: ... transiently transfected HEK293T cells were starved for 1h in methionine-free RPMI (Thermo Fisher Scientific) and subsequently pulsed for 30 minutes in starvation medium supplemented with 200µCi/mL EasyTagTM EXPRESS 35S protein labeling mix (Perkin Elmer) ...
-
bioRxiv - Genomics 2024Quote: ... for 1h at 37°C and Proteinase K Solution (20 mg/mL, Thermo Fisher Scientific) for 1h at 55°C ...
-
bioRxiv - Microbiology 2023Quote: ... for 1h and revealed using a 1/300 dilution of FITC-conjugated streptavidin (SNN1008, Invitrogen). Staining of DNA was performed on fixed cells by incubating them for 5 min in a 1 μg/ml 4,6-diamidino-2-phenylindole (DAPI ...
-
bioRxiv - Molecular Biology 2021Quote: ... Primers were designed using Methyl Primer Express®Software v1.0 (Applied Biosystems/ThermoFisher Scientific) to amplify specific regions of the genome following bisulphite conversion ...
-
bioRxiv - Molecular Biology 2021Quote: ... Primers were designed using Methyl Primer Express®Software v1.0 (Applied Biosystems/ThermoFisher Scientific) to amplify specific regions of the genome following bisulphite conversion ...
-
Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: Membrane potential was evaluated using the image-iT TMRM (tetramethylrhodamine, methyl ester) reagent (Invitrogen) following the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... They were then incubated with Tetramethylrhodamine-methyl-ester (TMRM) (Thermo Fisher Scientific, United States) for 30 min ...
-
bioRxiv - Developmental Biology 2022Quote: ... Slides were cleared in methyl salicylate and mounted using DPX mountant (Fisher Scientific, #18404). Images of whole mounts were captured at different magnifications using stereoscope (Magnus MSZ-TR).
-
bioRxiv - Microbiology 2022Quote: Membrane potential was evaluated using the image-iT TMRM (tetramethylrhodamine, methyl ester) reagent (Invitrogen) following the manufacturer’s instructions ...
-
bioRxiv - Physiology 2020Quote: ... or TMRM (Tetramethylrhodamine, methyl ester; 10 nM, 37°C) (Molecular Probes, Invitrogen, Paisley, UK). Following treatment ...
-
bioRxiv - Physiology 2020Quote: ... or TMRM (Tetramethylrhodamine, methyl ester; 10 nM, 37°C) (Molecular Probes, Invitrogen, Paisley, UK). Following treatment ...
-
bioRxiv - Neuroscience 2022Quote: ... gels were incubated with 10 µM BODIPY TR Methyl Ester (Thermofisher, catalog no. C34556) in 1× PBS for 1 h on a rocking platform at RT and washed three times with 1× PBS for 30 min each ...
-
bioRxiv - Neuroscience 2022Quote: ... gels were incubated with 10 µM BODIPY TR Methyl Ester (Thermofisher, catalog no. C34556) in 1× PBS for 1 h on a rocking platform at RT and washed three times with 1× PBS for 30 min each ...
-
bioRxiv - Physiology 2022Quote: ... 60 μL of N-methyl-N-trimethylsilyltrifluoracetamide (MSTFA with 1%TMCS, ThermoFisher Scientific #TS48913) was added automatically via the auto sampler and incubated for 30 minutes at 37 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... The crude peptide was precipitated in tert-butyl methyl ether (TBME, ACROS Organics, AC378720025). After the supernatant was removed by centrifugation ...
-
bioRxiv - Biochemistry 2023Quote: ... N-methyl-N-(trimethylsilyl)-triAuoroacetamide (TMS) and ammonium acetate were purchased from Thermo Fisher Scientific (Waltham) ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were washed with PBS 3-5 times for 5 minutes each and mounted with ProLong Gold Antifade Mountant (#P36930, Thermo Fisher Scientific, Waltham MA). Confocal images were taken with the 20x and 40x (oil ...