Labshake search
Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... brain sections from 3- to 5-month-old mice were exposed to 2.5 µM MitoSOX Red (Molecular Probes), at 37°C for 30 min14 ...
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bioRxiv - Plant Biology 2020Quote: ... The solution was sonicated for 3-5 mins using an ultrasonic cleaner (FS220, Thermo Fisher Scientific, Waltham, MA). The solution was diluted to 10 mL with 2% nitric acid ...
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bioRxiv - Cell Biology 2021Quote: ... 5 to 20 ng of RNA were amplified and labelled with the GeneChip 3’ IVT Pico Kit (Affymetrix) according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 3 to 5 min in 100 μM Lysotracker-Red DND-99 (Life Technologies, Carlsbad, CA, USA)[49] and 1 μM Höchst 33342 (Thermo Fisher Scientific Inc ...
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bioRxiv - Genomics 2020Quote: ... slides were washed 3 times for 5 min with TNT and mounted with Prolong Gold (P36930, Life Technologies).
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bioRxiv - Plant Biology 2021Quote: ... The bands were detected by using SuperSignal pico/femto chemiluminescence kits (Thermo Scientific; #’s1859022/3 and 1859674/5).
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bioRxiv - Biochemistry 2020Quote: ... 3 μg/mL gentamicin (Wisent) and 5 ng/mL recombinant mouse fibroblast growth factor basic (FGF-b, ThermoFisher). At ~90% confluence ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 minutes prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Immunology 2020Quote: ... 5% CO2 and medium were changed every 3 days and cells were passaged with 0,05% trypsin-EDTA (Invitrogen) twice per week.
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bioRxiv - Genomics 2020Quote: ... Biotin labelled fragments which contained the 5’ and 3’ connecting region was enriched using Dynabeads M280 streptavidin (Invitrogen) and incubating at room temperature for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: 5’ - capped and 3’- polyadenylated hCas9-2xNLS mRNA was transcribed in vitro using mMessageMachine T7 (ThermoFisher, Cat# AM1344) and Poly(A ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time PCR (RT-PCR) were performed on a QuantStudio 3 or QuantStudio 5 machines (Thermo Fisher Scientific). Primers/probe sets from Applied Biosystems were selected from the Thermo Fisher Scientific website (Supplementary Table 3) ...
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bioRxiv - Genetics 2023Quote: ... 3-5 million cells or approximately 15 ug of DNA crosslinked with 2% PFA (Fisher Scientific F79-500) were used as input per reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... An RNA linker (5’-CACGACGCUCUUCCGAUCU-3’) was ligated to the polyA RNA with T4 RNA ligase (Thermo Scientific) to capture RNAs with a 5’ monophosphate (5’P) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 min prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Slides were again washed with PBS-/- 3×5 min and mounted using Prolong Gold Antifade Mountant (Thermo Scientific). Sections stained with secondary only antibodies were included to determine fluorescence threshold before image acquisition in each channel ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Sections were washed 3 times 5 minutes in PBS and mounted using ProLong Gold mouting media (Thermo Fisher) under glass cover slips.
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bioRxiv - Microbiology 2024Quote: ... exponentially growing cells in suspension were diluted to 3×109 and 5 µl spotted on 1.5% agar (Gibco) supplemented with 0.5% CTT and immediately covered with a coverslide ...
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bioRxiv - Developmental Biology 2023Quote: ... rtf1-e3 gRNA (target sequence: 5’-GGAAGAAGGGGAAACCGAGCAA-3’) was synthesized using the MEGAshortscript T7 Transcription Kit (ThermoFisher Scientific) and purified using the RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... washed 3 x 5 min with PBS and incubated with Alexa Fluor-488 goat anti human secondary (Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed 3 x 5 min with TBST and developed using chemiluminescent substrate (Thermo Scientific SuperSignal West) and imaged using a BioRad Chemidoc.
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bioRxiv - Neuroscience 2023Quote: ... and then washed with PBS 3 x 5 min and mounted with ProLong Gold Antifade Mountant (ThermoFisher, P36930). Mounted coverslips were then imaged on a Zeiss Axio Observer-Z1 widefield microscope equipped with a Zeiss Plan-Apochromat 63x / 1.4 Oil objective ...
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bioRxiv - Cell Biology 2023Quote: The full length Aff3ir mRNA was cloned using commercially available 5’-RACE and 3’-RACE kits (ThermoFisher Scientific) according to the protocols provided ...
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bioRxiv - Biochemistry 2022Quote: ... 2-3 µL of 5 µM protein solution was introduced directly into Q-Exactive UHMR mass spectrometer (ThermoFisher) through gold coated capillary needles that were prepared in-house30 ...
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bioRxiv - Bioengineering 2023Quote: ... iPSCs were passaged in clumps at ∼ 70% confluency every 3 – 5 days by dissociation with Versene (Life Technologies) at typical ratios between 1:6 and 1:12 ...
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bioRxiv - Physiology 2024Quote: ... 1x 3–5 min with PBS + 1ng/mL DAPI and mounted in ProLong Gold antifade reagent (Life Technologies).
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bioRxiv - Cell Biology 2024Quote: ... Slides were washed 3 × 5 min in PBS and embedded into ProLong Diamond Antifade Mountant (Thermo Fisher Scientific). For control experiments ...
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bioRxiv - Synthetic Biology 2024Quote: ... Passaging was performed by brief (3-5 minute) treatment with 0.5 mM EDTA and 0.25X TRYPLE Express (Gibco) in phosphate-buFered saline (PBS) ...
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RNA Binding of GAPDH Controls Transcript Stability and Protein Translation in Acute Myeloid LeukemiabioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from 3-5 × 106 cultured MOLM-13 cells using TRIzol reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The same test was done in parallel to assess the cell viability using MTT at 5 ug/mL (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (Invitrogen). Sixteen sites per well were acquired using a 20x Olympus objective plan fluorite NA 0.45 (1320517 ...
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bioRxiv - Cell Biology 2024Quote: ... We performed MTT (3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) assay (cat no. V13154; Thermo Fisher) for determining cell viability after different tunicamycin treatments as per the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... Cryo-STET data were collected using the STEM Tomography software (Fig. 2A, Fig. 3, Fig. 5; Thermo Fisher) or SerialEM (Fig ...
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bioRxiv - Evolutionary Biology 2024Quote: ... intestinalis was used for 3’RACE and 5’RACE experiments using FirstChoice™ RLM-RACE Kit (Invitrogen, AM1700) following the manufacturer’s protocol and with the following CiTCF-specific primers ...
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bioRxiv - Cell Biology 2024Quote: ... as such: position (636-653 bp) 5’ aCAcTAtCAgAGgGAtAT 3’ (modified region corresponding to siRNA from Thermo Fisher s38909) and position (1235-1252 bp ...
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bioRxiv - Cell Biology 2024Quote: ... as such: position (111-131 bp) 5’- GAaTCgAGtCTaGAgTCtAgc-3’ (modified region corresponding to siRNA from Thermo Fisher s226949) and position (607-627 bp ...
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bioRxiv - Cell Biology 2024Quote: ... as such: position (1411-1431 bp) 5’ aGaAAaCTgCTgAGgGTgTTa 3’ (modified region corresponding to siRNA from Thermo Fisher s228499) and position (2164-2187 bp ...
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bioRxiv - Neuroscience 2022Quote: ... dried and cover slipped under glycerol:TBS (3:1) with Hoechst 33342 (1:1000; ThermoFisher Scientific). Slides were imaged at 10X magnification on a slide scanning microscope Olympus VS120 slide scanning microscope (Olympus ...
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bioRxiv - Microbiology 2023Quote: ... 3 μL of 1 mg mL−1 was vitrified using a Mark IV Vitrobot (Thermofisher). Prior to sample vitrification ...
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bioRxiv - Cell Biology 2020Quote: ... in a 3:1 ratio with 1X penicillin/streptomycin (Gibco; 15070) and 5% FBS (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... rat Taste receptor type 1 member 3 (Tas1r3, Rn00590759_g1, Applied Biosystems) and rat Taste receptor ...
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bioRxiv - Cell Biology 2020Quote: ... and washed 3 times with 1x PBS (Fisher Scientific, BP399-1).
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bioRxiv - Microbiology 2020Quote: ... and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5,000 dilution, Invitrogen). Secondary antibodies against rabbit and mouse IgG conjugated to IRDye 680LT or IRDye 800CW were obtained from Li-Cor (1:10,000 dilution) ...
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bioRxiv - Cancer Biology 2022Quote: ... BxPC-3 and PSN-1 were cultured in RPMI1640 (Thermo Fisher), supplemented with 10% FCS and 1% P/S ...
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bioRxiv - Microbiology 2022Quote: ... 1 nM TO-PRO™-3 Iodide (642/661 nm, Thermofisher) and 8 ng/ml calcofluor-white (CW ...
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bioRxiv - Microbiology 2022Quote: ... 1 nM TO-PRO™-3 Iodide (642/661 nm, Thermofisher) and 8 ng/ml calcofluor-white (CW ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were collected into FAD media (DMEM/F12 3:1 (Gibco) supplemented with 10% FBS ...
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bioRxiv - Genomics 2021Quote: ... for RPE1] in 1:3 ratio in Opti-MEM media (Gibco), incubated for 15 min at RT ...
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bioRxiv - Neuroscience 2022Quote: Neurons were transfected at DIV 1-3 using Lipofectamine 2000 (Invitrogen). For one Ø18-mm coverslip seeded with 100,000 neurons ...
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bioRxiv - Immunology 2021Quote: ... and goat anti-mouse Cyanine 3 (Life technologies, A10521, 1:1000). Images for BrdU staining were taken using a Zeiss Confocal (LSM710 META) ...
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bioRxiv - Microbiology 2020Quote: ... 3 μl of DNA ladder (1 Kb Plus DNA Ladder; Invitrogen), 6 μl of positive control and 10μl of template DNA were ran on 2.5% (w/v ...