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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... All other lines were passaged at approximately 80% confluency (every 4-5 days on average) as tiny clusters (3-5 cells on average) using 0.5 mM EDTA (15575020, Gibco, concentration 10 mM).
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bioRxiv - Cell Biology 2021Quote: ... The βarr1/2 siRNA (5’-ACCUGCGCCUUCCGCUAUG-3’) and a scrambled siRNA (control, 5’-UGGUUUACAUGUCGACUAA-3’) (Dharmacon) were transfected by RNAimax (Invitrogen) according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2020Quote: ... After the fourth EDTA incubation the pieces were cut into 2 mm2 pieces and placed in 5 mL digestion solution containing 5% fetal bovine serum (Gibco), 10 mM HEPES (Gibco) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples (5 μl) were injected on a C18 PepMap trap column (5 μm, 100 μm I.D. x 2 cm, Thermo Scientific) at 10 μl/min ...
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bioRxiv - Biochemistry 2022Quote: ... Samples (5 μl) were injected on a C18 PepMap trap column (5 μm, 100 μm I.D. x 2 cm, Thermo Scientific) at 20 μl/min ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated for 2-24hrs at 37°C 5% CO2 +/- myristoylated aPKC pseudosubstrate inhibitor (5 μM; Invitrogen; Product number 77749) in IMDM+0.1% BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Samples (5 μL) were injected on a C18 PepMap trap column (5 µm, 300 µm I.D. x 2 cm, Thermo Scientific) at 20 µL/min ...
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bioRxiv - Biochemistry 2024Quote: ... were maintained by continuous culture at 2-5% hematocrit in human erythrocytes with malaria culture medium (RPMI 1640 supplemented with 5 g/L Albumax II (Gibco), 0.12 mM hypoxanthine (1.2 ml 0.1 M hypoxanthine in 1 M NaOH) ...
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bioRxiv - Cancer Biology 2024Quote: ... The relative expression of each target was calculated using the relative quantification method (2-ΔΔCT) with RNA18S (5’-TGTGGTGTTGAGGAAA-GCAG-3’ and 3’-TCCAGACCATTGGCTAGGAC-5’; Invitrogen) as internal control ...
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bioRxiv - Molecular Biology 2021Quote: ... Primers were designed using Methyl Primer Express®Software v1.0 (Applied Biosystems/ThermoFisher Scientific) to amplify specific regions of the genome following bisulphite conversion ...
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bioRxiv - Molecular Biology 2021Quote: ... Primers were designed using Methyl Primer Express®Software v1.0 (Applied Biosystems/ThermoFisher Scientific) to amplify specific regions of the genome following bisulphite conversion ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... They were then incubated with Tetramethylrhodamine-methyl-ester (TMRM) (Thermo Fisher Scientific, United States) for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were cleared in methyl salicylate and mounted using DPX mountant (Fisher Scientific, #18404). Images of whole mounts were captured at different magnifications using stereoscope (Magnus MSZ-TR).
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bioRxiv - Microbiology 2022Quote: Membrane potential was evaluated using the image-iT TMRM (tetramethylrhodamine, methyl ester) reagent (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... or TMRM (Tetramethylrhodamine, methyl ester; 10 nM, 37°C) (Molecular Probes, Invitrogen, Paisley, UK). Following treatment ...
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bioRxiv - Physiology 2020Quote: ... or TMRM (Tetramethylrhodamine, methyl ester; 10 nM, 37°C) (Molecular Probes, Invitrogen, Paisley, UK). Following treatment ...
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bioRxiv - Microbiology 2023Quote: ... Tris(hydroxymethyl)methyl-3-amino propane sulfonic acid (TAPS) was purchased from Acros Organics. Mal-PEG ...
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bioRxiv - Neuroscience 2022Quote: ... gels were incubated with 10 µM BODIPY TR Methyl Ester (Thermofisher, catalog no. C34556) in 1× PBS for 1 h on a rocking platform at RT and washed three times with 1× PBS for 30 min each ...
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bioRxiv - Neuroscience 2022Quote: ... gels were incubated with 10 µM BODIPY TR Methyl Ester (Thermofisher, catalog no. C34556) in 1× PBS for 1 h on a rocking platform at RT and washed three times with 1× PBS for 30 min each ...
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bioRxiv - Physiology 2022Quote: ... 60 μL of N-methyl-N-trimethylsilyltrifluoracetamide (MSTFA with 1%TMCS, ThermoFisher Scientific #TS48913) was added automatically via the auto sampler and incubated for 30 minutes at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The crude peptide was precipitated in tert-butyl methyl ether (TBME, ACROS Organics, AC378720025). After the supernatant was removed by centrifugation ...
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bioRxiv - Biochemistry 2023Quote: ... N-methyl-N-(trimethylsilyl)-triAuoroacetamide (TMS) and ammonium acetate were purchased from Thermo Fisher Scientific (Waltham) ...
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bioRxiv - Cell Biology 2024Quote: ... mouse monoclonal antibody to Histone H3 (Tri methyl K79) (BSM-33098M, Thermo Fisher Scientific). Secondary antibodies were goat anti-rabbit or goat anti-mouse labeled with Alexa Fluor 488 or Cy5(Servicebio) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 11.3 mM Nα-benzoyl-L-arginine methyl ester carbonate (Fisher Scientific, 50-501-393), 200 µM soybean trypsin inhibitor (Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... PAA gel surface activation reagents were ethyl(dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) solution (Fisher Scientific). Collagenase from Clostridium histolyticum was purchased from Sigma.
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bioRxiv - Cell Biology 2021Quote: ... and iPSC-CM for 35 minutes with tetramethylrhodamine ethyl ester (TMRE, 20 nM, Invitrogen, Cat# T-669) and Mito Tracker Green (MTG ...
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bioRxiv - Pathology 2020Quote: The mitochondrial membrane potentiality was measured using a TMRE (Tetramethylrhodamine ethyl ester, ThermoFisher Scientific, Cat. No. T669) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... Grids were blotted for 2-4 seconds at a blotting force of 4 and plunge-frozen in liquid ethane using a MarkIV Vitrobot (Thermo Fisher Scientific). The chamber was maintained at 8 °C and 100% humidity during freezing ...
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bioRxiv - Immunology 2020Quote: ... 2-4 × 105 cell equivalents per well were loaded into a NuPAGE 4-12% Bis-Tris density gradient gel (Thermo Fisher Scientific) and ran at a constant 150V for 80 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... cells were loaded with the calcium indicator Fluo-4 by incubating in supplemented NB medium that contained 2 µM of Fluo-4 AM (Invitrogen, Carlsbad, CA), and Pluronic F-127 (0.04% ...
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bioRxiv - Cancer Biology 2020Quote: ... The medium was changed every 2-3 days and organoids were passaged every 2-4 weeks by dissociation with 1 ml of TrypLE Express (Life Technologies) at 37°C for 5 min ...
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bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
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bioRxiv - Immunology 2020Quote: T or B cells (2-4 × 107) obtained from 2 spleens of actin-GFP mice by negative MACS kits (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lot 134874), and 4-(2-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, purity ≥ 99.%, CAT #BP310-1, Lot 052975) were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cancer Biology 2023Quote: ... were mixed in a 3:1 with sample buffer (NuPAGE™ LDS Sample Buffer (4X) + 2-Mercaptoethanol in 2:1) and loaded onto a precast Gel (NuPAGE™ 4-12% Bis-Tris Gel, Invitrogen). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Bioengineering 2024Quote: Coronal sections of healthy murine brain were prepared and stained as previously described101 either using 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng mL−1; Molecular Probes) to stain cell nuclei or primary antibodies for rabbit anti-NeuN (1:1000) ...
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bioRxiv - Bioengineering 2023Quote: ... The concentrated virus was diluted 1:5 in hepatocyte medium containing N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid buffer (HEPES; 20 mM; Gibco) and 4 μm/mL polybrene (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... with IVT mix (0.5 µL Ribolock inhibitor, Invitrogen, 2 µL T7 polymerase buffer, NEB, 8 µL rNTP mix, 2 µL T7 polymerase, Invitrogen). DNA was removed by addition of 1 µL turbo DNase (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... permeabilised with 0.5% Triton x-100 and OPP incorporation into newly synthesised peptides was measured by conjugating with Alexa647-azide via a Click-IT chemistry reaction (2 µM Alexa-647-azide, 2 mM CuSO4, 5 mM ascorbic acid in PBS) (Invitrogen) and assessing fluorescence by flow cytometry.
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bioRxiv - Biophysics 2024Quote: ... were seeded in a 6-well culture plate at a concentration of 2 x 105 cells ml-1 (2 ml per well in DMEM (Nissui) supplemented with 5 % fetal bovine serum (Gibco), glutamine ...
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bioRxiv - Microbiology 2020Quote: Mice were injected with 100 μg of 5-ethynyl-2’deoxyuridine (EdU, Invitrogen) 2 hours prior to splenocyte harvest ...
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bioRxiv - Developmental Biology 2021Quote: ... with 50-100 nl of 1 mM 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen) at stage 41 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were counterstained with 2 μM CellTracker Green (5-chloromethylfluorescein diacetate dye) (ThermoFisher) followed by NucBlue (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... (B-5-1-2 coupled to Alexa-488, Thermo Fisher or DM1A, Sigma), rat anti-tyrosinated tubulin (YL1/2 ...
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bioRxiv - Microbiology 2020Quote: ... 5 mM tris(2-carboxyethyl)phosphine (TCEP, Bond-Breaker, neutral pH, Thermo Scientific) was added and samples were incubated at room temperature for 20 min ...
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bioRxiv - Microbiology 2021Quote: ... mice were injected with 100 μg of 5-ethynyl-2’deoxyuridine (EdU, Invitrogen) 3.5 hours prior to splenocyte harvest ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl) phosphine hydrochloride (Thermo Fisher) for 30 min and alkylated with 10 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 mM succinimidyl 3-(2-pyridyldithio)propionate (SPDP) (Thermo Fisher Scientific, USA) in PBS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... monensin (5 μM) and retro-2 (100 μM) were obtained from Molecular Probes [B7450] ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were blocked for 2 hr with 5% goat serum (Thermo Fisher Scientific) and 5% bovine serum albumin (BSA ...