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Citations for Thermo Fisher :
9101 - 9150 of 10000+ citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... with a replacement in the N-terminal domain loop 8 (139CQKRDGPH146 to 139AEAG142, designated as rA3GR8) was constructed in the pET-sumo vector (Thermo Fisher Scientific). This construct generated a SUMO fusion with an N-terminal 6xHis tag and a PreScission protease cleavage site ...
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bioRxiv - Biochemistry 2024Quote: ... and cloned into a pRK5-derived plasmid with a C- or N-terminal FLAG tag using the gateway vector conversion system (Invitrogen, cat # 11828029). All gene constructs were verified by DNA sequencing.
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bioRxiv - Bioengineering 2024Quote: ... which in turns intercalates with nucleic acids and emits red fluorescence detectable by microscopy.38 Frozen tissue sections from ATA samples (n=4 per group) were stained with DHE (10µM; D1168, Invitrogen; Carlsbad, CA, USA) in a dark humidified incubator at 37◦C for 20 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified MuSCs from each replicate were stained for viability with calcein-AM and ethidium-homodimer1 (P/ N L3224 Thermo Fisher Scientific). scRNA libraries were generated using the GemCode Single-Cell Instrument (10x Genomics ...
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bioRxiv - Cancer Biology 2023Quote: Ewing sarcoma A673 (ATCC CRL-1598) and neuroblastoma SK-N-AS (ATCC CRL-2137) cell lines were grown in DMEM (Gibco, #31966-021) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... The Flag plasmids were transiently transfected in to N/Tert-1 cells using Lipofectamine™ RNAiMAX transfection (Invitrogen, catalog no. 13778-100) protocol and transfected in to U2OS cells using the calcium phosphate transfection protocol.
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bioRxiv - Biochemistry 2023Quote: ... Full-length genes of human RNGTT and CMTR1 were cloned into pMlink vector containing a 4×ProteinA tag at the N-terminal and were transfected to Expi293F cells (Thermo Fisher Scientific) when cell density is 2.5× 106 /ml ...
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bioRxiv - Cancer Biology 2023Quote: ... and the total protein concentration was determined using a Pierce Reducing Agent Compatible BCA kit (RAC-BCA, Thermo Scientific, P/N 23252) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Appropriate correction factors for the individual TMT channels for both lysine side-chain labelling and peptide N-terminal labelling as per the TMT-6plex kits used (Thermo Fisher Scientific) were configured into the database search ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was isolated from MG63 lysates (n = 2 per treatment condition) using the PureLink RNA Mini kit (12183018A, Thermo Fisher Scientific) with DNase set (12185010 ...
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bioRxiv - Cell Biology 2023Quote: Liquid chromatography separation was performed at a flow rate of 300 nL/mL on a C18 pre-column (Acclaim PepMapTM 100, 75 μm×2 cm, nanoViper, P/N 164946, ThermoFisher Scientific Incorporation) followed by analytical column (Acclaim PepMapTM RSLC C18 ...
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bioRxiv - Cancer Biology 2023Quote: Wild-type and mutant (C139A) USP39 with a Flag-tag at its N-terminal were cloned into the pLenti6/V5 lentiviral vector (Invitrogen, California, USA). Specific shRNA oligonucleotides targeting USP39 were cloned into the pLL3.7 lentiviral vector (Addgene) ...
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bioRxiv - Genetics 2023Quote: ... The optimized and minimum required protocol for organoid hepatic isolation consisted of rinsing the fresh tissue in Phosphate Buffered Saline (PBS)/N-acetylcysteine (NAC) once and then transferring it to a tube filled with Advanced DMEM/F12 (Gibco; 12634-010), mincing the tissue into small fragments ...
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bioRxiv - Cell Biology 2023Quote: ... at a density of 107 cells in 19 ml of medium for dual-proteomics or at 1.5 x 104 cells per well in 96-well plates (Nunc, cat n° 164590) in 0.2 ml of medium for MTT ...
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bioRxiv - Genomics 2023Quote: ... coated dishes in N2 medium (high-glucose DMEM, 1% N-2, 2% B-27 and 1% penicillin/streptomycin, from Thermo Fisher Scientific) at a density of 2×105 cells/cm2 ...
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bioRxiv - Immunology 2022Quote: ... which was created via the in vitro T7-DNA-dependent RNA transcription of a linearized DNA molecule containing the target region of the N gene full-length by using MEGAscript T7 Transcription Kit (ThermoFisher, Cat# AM1334), and purified with MEGAclear Transcription Clean-Up Kit (ThermoFisher ...
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bioRxiv - Developmental Biology 2023Quote: ... Two month old kidneys were harvested and isolated kidney cortex was cross-linked with 2 mM Di (N succinimidyl) glutarate (DSG, Proteo Chem C1104) in PBS for 30 min followed incubation in 1% formaldehyde (Thermo Scientific 28906) for an additional 10 min at room temperature and quenched with 125 mM glycine for 5 min ...
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bioRxiv - Developmental Biology 2023Quote: ... both diluted 1:50 in DMEM/F-12 or with 20 ug/mL recombinant truncated vitronectin (VTN-N) (Thermo Fischer Scientific, A31804) diluted in DMEM/F-12 (Gibco, 21331-020) for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by the sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) at 150V for 2h using NuPAGE 2-(N-morpholino)ethanesulfonic acid SDS running buffer (NP0002, Thermo Fisher Scientific), NuPAGE Antioxidant (NP0005 ...
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bioRxiv - Plant Biology 2023Quote: ... The roots were lyophilized in vacuo and analyzed for total N and 15N content using elemental analysis–isotope ratio mass spectrometry (Flash EA1112-DELTA V PLUS ConFlo III system, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... Infectious titers were determined by focus-forming unit assay whereby foci of infection were measured using anti-nucleocapsid (N) antibody (Invitrogen; MA1-7403). Where indicated ...
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bioRxiv - Cancer Biology 2023Quote: ... the co-localization of bacterial biomass and necrotic tissue was assessed by a pathologist in samples with high bacterial biomass (n = 21) using an EVOS M7000 microscope (Thermofisher MA, USA).
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bioRxiv - Biochemistry 2023Quote: ... Dried samples were redissolved with 300 μL of 0.1% TFA in H2O and further fractionated using high-pH reversed-phase peptide fractionation kits (ThermoFisher, P/N 84868) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... GFP images or RFU were collected at indicated time points post-infection by a fluorescence microscope (MI52-N) or a Varioskan LUX Multi-well Luminometer (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: Striatum tissues from Cacna1dG407R/+ mice and wildtype siblings were collected and homogenized in N-Per Neuronal protein extraction buffer (Thermo Fisher Scientific) supplemented with Protease inhibitors (Halt Protease and Phosphatase Inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... and inserted with an N-terminal FLAG-tag via NheI and NotI restriction sites into the tetracycline-inducible pcDNA5/FRT/TO vector (Thermo Fisher Scientific). For stable integration ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 x 105 cells were in seeded in 6-well plate and after 24 h 1000 ng px330-BbsI-PITCh-UPF1-N and 500 ng donor plasmid pCRIS-PITChv2-PurR-FLAG were transfected using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from 5 dpf wild type and vps4a mutant larvae (n = 15-20 each genotype) using TRIzol reagent (Thermo Fisher, 15596026) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... and once with 1X PBS for 5 min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP ...
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bioRxiv - Immunology 2024Quote: In vivo labeling of tumor-infiltrating T lymphocytes (TILs) with 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG, Life Technologies, catalog: N13195) was done by intravenous (retroorbital ...
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bioRxiv - Cell Biology 2024Quote: ... Tryptic peptides (2 µg) were loaded onto a C18 trap (75 µm × 2 cm; Acclaim PepMap 100, P/N 164946; Thermo Fisher Scientific) at a flow rate of 2 µL/min of solvent A (0.1% formic acid and 3% acetonitrile in LC-mass spectrometry grade water) ...
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bioRxiv - Neuroscience 2024Quote: ... into the medial septal area (MS) of C57Bl/6NTac mice (n=6). For the colocalization experiments (Fig. 1) we injected yellow-green FluoSpheres (Thermo Fisher Scientific) into the MS of two C57Bl/6NTac mice or AAVrg-syn-FLEX-jGCaMP8m-WPRE (Addgene ...
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bioRxiv - Neuroscience 2024Quote: We derivatized the dried polar and protein samples using methoxyamine HCl (20 mg/mL) in pyridine and N-methyl-trimethylsilyl-trifluoroacetamide (MSTFA; Thermo Fisher Scientific). For the polar fraction ...
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bioRxiv - Microbiology 2020Quote: ... which were maintained in Minimal Essential Medium (MEM) Rega-3 (Thermo Fisher Scientific) + 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... with single-cell passaging every 2-3 days using accutase (ThermoFisher Scientific A1110501) at split ratios between 1:5 and 1:12.
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bioRxiv - Cell Biology 2020Quote: ... Coverslips were washed 3 times in PBS and secondary antibodies (Oregon Green - Thermofisher O-6382 ...
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bioRxiv - Immunology 2021Quote: ... qPCR was performed on the QuantStudio 3 Real-Time PCR System (Applied Biosystems) using SYBR Green ...
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bioRxiv - Immunology 2021Quote: ... on the QuantStudio 5 or QuantStudio 3 Real-Time PCR System (ThermoFisher Scientific). Relative transcript levels were normalized to TATA-binding protein (Tbp ...
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bioRxiv - Plant Biology 2020Quote: ... from 2-3 weeks old seedlings and 500ng was treated with DNaseI (Ambion) and used for reverse transcription ...
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bioRxiv - Genetics 2020Quote: ... to add 3’A overhangs prior to TOPO TA-cloning (K4600-01, Invitrogen). We then performed colony PCR using the same PCR primers and conditions used to amplify the hmx1 sequence from cDNA ...
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bioRxiv - Molecular Biology 2020Quote: ... according to manufacturer’s instructions and run on a Quant Studio 3 System (ThermoFisher) using the default settings for SYBR Green reagents and the fast run mode in the QuantStudio Design & Analysis Software v.1.4.2 ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were performed on a QuantStudio 3 Real-Time PCR System (Thermo Fisher) with the amplification conditions as previously described (14).
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bioRxiv - Molecular Biology 2021Quote: ... and CAF-1 p60 (5′-AAUCUUGCUCGUCAUACCA-3′) were transfected using RNAi MAX (Invitrogen).
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bioRxiv - Developmental Biology 2021Quote: ... These lines were routinely passaged every 3-4 days using Versene (ThermoFisher; 15040066). All lines were routinely screened for differentiation and tested for mycoplasma contamination.
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bioRxiv - Neuroscience 2020Quote: ... and 0.175 g/ml 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) (Invitrogen). Alkaline phosphatase staining reaction was proceeded o/n at RT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Gels were stained for 3 minutes with SYBR Gold (Thermo Fisher Scientific S11494), and the region corresponding to the 18-50 nucleotide sized fragments excised ...
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... and TO-PRO®-3 (1:3000, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Developmental Biology 2021Quote: ... qPCR was done on QuantStudio 3 Real Time PCR Systems (Thermo Fisher Scientific) with the TaqMan gene expression Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... or a positive control probe 5’-5Alexa488N/(ATA)8TUU (ATA)7-3’ (Invitrogen). Reactions were incubated in a water bath at 37°C for 2 hrs ...
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bioRxiv - Microbiology 2022Quote: ... JEG-3 (ATCC) were cultured in EMEM (ATCC) supplemented with 10% FBS (Gibco), penicillin-streptomycin-glutamine (Gibco) ...