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Citations for Thermo Fisher :
9101 - 9150 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... The purified SEP was passed through a 0.2 µm pore size syringe filter for sterilization and quantified using the BCA Protein Assay kit (Thermo Fisher Scientific, Les Ulis, France). Aqueous solutions were stored at -20°C until further use.
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bioRxiv - Cancer Biology 2023Quote: Cell death was assessed using the Click-iT™ Plus TUNEL Assay for In Situ Apoptosis Detection Alexa Fluor™ 647 Imaging Kit (Life Technologies, C10617). Unless otherwise stated ...
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bioRxiv - Microbiology 2022Quote: ... the resulting supernatant of each sample was transferred to a labeled microcentrifuge tube and stored at -20°C The Quant-iT dsDNA Assay Kit (Thermo Fisher Scientific, MA, USA) was used to measure DNA concentrations prior to PCR amplification ...
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bioRxiv - Plant Biology 2023Quote: ... Final libraries were assessed for size and quantity using an Agilent DNA1000 chip and a Qubit® dsDNA HS Assay Kit (Invitrogen, Carlsbad, California, USA), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 42 commercial antibodies (see Table S2 for catalogue numbers and dilution detail) were buffer-exchanged with PBS and quantified using the Pierce® BCA Protein Assay Kit (23227, Thermo Scientific, Waltham, US). Approximately 1 nl was printed per feature on Nexterion H amine-reactive hydrogel-coated glass slides using a SciFLEXARRAYER S3 piezoelectric printer (Scienion ...
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bioRxiv - Genomics 2022Quote: ... EdU staining was performed on thawed nuclei using Click-iT Plus EdU Alexa Fluor™ 647 Flow Cytometry assay Kit (Thermo Fisher Scientific, 10634). A 500 µL reaction buffer (prepared following the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... EdU staining was performed on thawed nuclei using Click-iT Plus EdU Alexa Fluor™ 647 Flow Cytometry assay Kit (Thermo Fisher Scientific, 10634). A 500 μL reaction buffer (prepared following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and ventral hippocampus were quantified using a Pierce Microplate BCA Protein Assay Kit (Thermo Scientific Pierce Protein Biology, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... mice were sacrificed 24 h post exposure to Cl2 or air and lavaged as described previously(7, 8) Total protein concentrations in cell-free lavage were measured with the BCA Protein Assay Kit (#23225; Thermo Fisher Scientific, Rockford, IL). Cells were re-suspended in 100μl of phosphate buffered saline (PBS ...
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bioRxiv - Physiology 2023Quote: ... The supernatant (islet sample) was collected and the total protein was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... DNA concentration was assessed by fluorometry using a Qubit™ 4 and Qubit™ 1X dsDNA HS Assay Kit (ThermoFisher Scientific, Waltham, MA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Labeling and detection of apoptotic cells were completed using the Invitrogen Click-iT Plus TUNEL Assay for In Situ Apoptosis Detection 488 kit (Invitrogen, Waltham, MA, catalog #: C10617) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell cycle analysis was performed by flow cytometry and in the final hour of drug treatment 10 μM EdU was added to the media containing drug to accurately identify cells in S-phase using a Click-iT™ EdU flow cytometry assay kit (Thermofisher, Waltham, MA, USA). Cells were harvested and fixed by 4% paraformaldehyde (15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein pellets were subsequently re-solubilized in 40 uL of 5% SDS (v/v) and assayed for protein using the PierceTM BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA). 130-170ug of protein/sample (in a total volume of 33 uL) ...
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bioRxiv - Microbiology 2023Quote: ... DNA concentrations of all PCR products were measured using a Qubit® Flex Fluorometer with the Qubit dsDNA BR Assay Kit (Invitrogen, Waltham, MA, USA) and normalised to 2 ng/μL ...
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bioRxiv - Biochemistry 2022Quote: ... were centrifuged at 16,000 g for 30 minutes at 4 °C and the supernatant was collected for protein estimation using Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific; Cat# 23227). Equal amounts of liver protein (100 μg ...
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bioRxiv - Cell Biology 2022Quote: ... The intensity values were normalized by the total protein in each sample (determined by the Pierce™ BCA Protein assay kit, ThermoFisher Scientific, cat# 23225) before they were used for quantitative analysis.
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bioRxiv - Cancer Biology 2023Quote: β-galactosidase activity in the PB3 and MDA-MB-231 cells after different treatments were evaluated using the CellEvent™ Senescence Green Flow Cytometry Assay Kit (Thermo Fisher Scientific #C10841) and manufacturer’s protocols ...
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bioRxiv - Bioengineering 2023Quote: Total miR levels in extracted RNA samples were quantified using a Qubit miR Assay Kit and Qubit 3.0 Fluorimeter (Thermo Fisher Scientific, Waltham, MA, USA); the manufacturer’s protocols were employed.
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bioRxiv - Cancer Biology 2023Quote: ... the dried pellet was resuspended in 8 M urea and quantified using the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, USA). A 2 hour incubation with 10 mM DTT at 56 °C was followed by 40 mM iodoacetamide for 45 minutes in the dark at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were then transferred to new tubes and the concentration of proteome was determined using Pierce™ BCA Protein Assay kits (Thermo Scientific™, 23227). Next ...
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bioRxiv - Plant Biology 2023Quote: ... The quantity and quality of DNA was examined on 1% agarose gel and Quan-iT PicoGreen DsDNA assay kit (Life Technologies, Carlsbad, CA, USA) on a FLUOstar Omega fluorometer (BMG Labtech ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatant was collected and protein concentration was determined using the Pierce™ BCA protein assay kit following manufacturer’s instructions (Thermo Fisher Scientific, Massachusetts, USA). Each sample was separated by non-reducing SDS-PAGE with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... and the precise concentration was determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH, Darmstadt, Germany). Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina ...
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bioRxiv - Genetics 2023Quote: ... The size distributions and DNA concentrations of the libraries were evaluated on an Agilent Bioanalyzer High Sensitivity DNA Chip and the Qubit DNA Assay Kit on a Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, United States). DNA concentrations were measured using quantitative PCR prior to cluster generation on the Illumina cBotwhich is a highly advanced automated clonal amplification system at the core of the Illumina sequencing process (Illumina ...
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bioRxiv - Genomics 2023Quote: ... DNA size and concentration of the barcoded pools were quantified using Agilent 2200 TapeStation and Qubit4® 1x dsDNA HR Assay Kit (Invitrogen, Carlsbad, CA, USA), respectively ...
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bioRxiv - Microbiology 2023Quote: ... The quality of the RNAs used to prepare RNA-Seq libraries was checked with the Qubit RNA BR Assay Kit (Thermo Fisher, Waltham MA, USA). SMAT libraries ...
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bioRxiv - Microbiology 2023Quote: ... and the precise concentration was determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH, Darmstadt, Germany). Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina ...
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bioRxiv - Cell Biology 2023Quote: The protein synthesis in fly brains was assessed using the Click-iT Plus OPP Alexa Fluor® 594 Protein Synthesis Assay Kit (Thermo Fisher Scientific, C10457). Fly brains were dissected in Drosophila medium (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentrations were determined by comparison with a known concentration of bovine serum albumin using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA). 30 µg of lysate were loaded per lane into 10% Mini-PROTEAN TGX™ gel (BioRad ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Quantity and integrity of total RNA was determined using a Qubit® 3.0 Fluorometer the Qubit® RNA BR (Broad Range) Assay Kit (ThermoFisher Scientific Inc., USA) and an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and kept on ice and protein concentration measured with a Qubit™ protein BR assay kit (Thermo Fisher Scientific, Waltham, MA) and samples stored at − 80°C ...
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bioRxiv - Cell Biology 2024Quote: ... EdU incorporation during this period was detected using the Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (Thermo Fisher #C10632) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: ... A fraction of this neuronal mixture was taken to measure protein concentration through Pierce ™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, A53225) and the rest was used to isolate the biotinylated synaptic cleft proteins following the steps specified in Loh et al.24 In this sense ...
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bioRxiv - Neuroscience 2024Quote: ... The total protein concentrations for each sample of the cell and tissue lysates were determined using the Pierce™ BCA Protein Assay Kit (Thermofisher Scientific, Cat# 23225). 4X Laemmli Sample Buffer (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant containing the lysate was collected by centrifugation at 15,000 rpm for 20 min and quantified using Pierce™ BCA Protein Assay Kits (Thermo Fisher Sc., Cat#23227). Equal amounts of protein were mixed with SDS sample buffer and boiled at 95°C for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: The opening of mitochondrial permeability transition pore was measured using Invitrogen™ Image-IT™ LIVE Mitochondrial Transition Pore Assay Kit (Invitrogen, cat. I35103). Cells were cultured in 35 mm glass-bottom dishes overnight in an incubator with 5% CO2 at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Concentration of the libraries was determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH, Darmstadt, Germany). Sequencing was performed on the HiSeq4000 instrument (Illumina Inc. ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The concentrations of total protein and albumin in mouse BAL fluid were quantified following the manufacturer’s instruction via the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, MA, USA) and Albumin (BCG ...
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bioRxiv - Cell Biology 2024Quote: ... all libraries were quantified using the highly sensitive fluorescent dye-based Qubit® ds DNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) and sequencing was performed using the illumina NovaSeq 6000 system (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Library concentrations were first measured on Qubit Fluorumeter 2.0 using the Qubit™ 1X dsDNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) and then on the Fragment Analyzer using the High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Cells that had incorporated EdU were fluorescently labeled using the Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (Thermo Fisher #C10632) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... The quantity and quality of library were validated by using the Qubit fluorometer with the Qubit dsDNA HS assay kit (Life Technologies, Grand Island, NY) and Agilent Bioanalyzer 2100 with Agilent DNA 1000 Kit (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: ... The quality and quantity of the extracted DNA was analyzed with a Qubit 4.0 Fluorometer using the dsDNA high-sensitivity assay kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) and a Colibri spectrophotometer (Titertek Berthold ...
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bioRxiv - Genomics 2024Quote: ... DNA quality and yields were assessed on a NanoDrop 1000 and Qubit 4 Fluorometer with Qubit dsDNA BR Assay kit (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Microbiology 2024Quote: ... Parasites were then rinsed in PBSTx then incubated at 37°C in TdT reaction buffer from Click-iT™ Plus TUNEL Assay for In Situ Apoptosis Detection kit (Thermo Fisher Scientific C10617) for one hour ...
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bioRxiv - Developmental Biology 2021Quote: ... chicken TNF-α (LITAF, assay ID number Gg03364359_m1) and the reference gene chicken GAPDH (assay ID number Gg03346982_m1) (Thermofisher Scientific). The PCR cycle parameters were an initial denaturing step at 95 °C for 10 min followed by 45 cycles of 95 °C for 15 s and 60 °C for 1 min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Extracts were evaluated for presence of high-molecular weight DNA using gel electrophoresis (2% agarose) and quantified at 2µl per sample in 200µl assays using Qubit broad-range DNA fluorometry assays (Thermo Fisher Scientific). DNA (500-1000ng of DNA per sample in 50µl reactions ...
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bioRxiv - Biochemistry 2022Quote: ... Total protein content of each pre-cleared sample was determined using Pierce bicinchoninic acid assay protein assay (Thermo Fisher Scientific). To measure luciferase activity ...
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bioRxiv - Biochemistry 2020Quote: Protein biosynthesis assays were carried out using the click-it plus O-propargyl-puromycin (OPP) protein synthesis assay (ThermoFisher Scientific) according to the manufacturer’s instructions ...