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Citations for Thermo Fisher :
9051 - 9100 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The concentration of the bacteriocin was measured after each step of protein purification using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA).
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bioRxiv - Molecular Biology 2019Quote: ... protein assay was done for extracted protein quantification using Pierce™ BCA Protein assay kit according to the manufacturer’s protocol (cat#23225, Thermo Fisher Scientific, MA, US). For western blotting ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The concentration of the mitochondrial DNA standard was measured by Qubit 1XdsDNA HS Assay Kit with Qubit 4 Fluorometer (Thermo Fisher Scientific, Waltham, MA). For the experiment described in Supplemental Figure 4 ...
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bioRxiv - Plant Biology 2021Quote: ... Concentrations of proteins in plant leaves were determined according to the Pierce BCA Protein Assay Kit-Reducing Agent Compatible (Thermo Scientific, #23250, Rockford, IL).
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bioRxiv - Physiology 2019Quote: ... The total protein of the kidneys was extracted by centrifugation at 14,000g for 15 minutes at 4°C and protein concentration was determined using micro bicinchoninic acid (BCA) protein assay kit (Thermo Scientific, Rockford, Il, USA) according to manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2019Quote: ... and total plasma protein was quantified after a 1:600 dilution utilizing a Micro BCA™ Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA).
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bioRxiv - Microbiology 2019Quote: ... The samples of individual gels were pooled into approximately equimolar subpools as indicated by band intensity and measured with the Qubit dsDNA br Assay Kit (Life Technologies GmbH, Darmstadt, Germany). Sub pools were mixed in an equimolar fashion and stored at −20°C until sequencing.
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bioRxiv - Cell Biology 2021Quote: Cell cycle progression of NALM6 cells was assessed using Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (Invitrogen cat # C10425), following manufacturer instructions ...
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bioRxiv - Genetics 2020Quote: ... Human DNA was removed using DNase I and RNA concentration was measured using Qubit RNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). After human DNA-depletion ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The samples of individual gels were pooled into approximately equimolar subpools as indicated by band intensity and measured with the Qubit dsDNA br Assay Kit (Life Technologies GmbH, Darmstadt, Germany). Subpools were mixed in an equimolar fashion and stored at −20 °C until sequencing ...
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bioRxiv - Genetics 2021Quote: ... The concentration of total RNA was measured using the Qubit RNA Assay Kit in a Qubit 2.0 Flurometer (Life Technologies, Carlsbad, CA, United States). RNA samples that met the criteria of having an RNA integrity number (RIN ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated in lysis buffer for 30 min at 4°C with continuous shaking and then centrifuged to collect clear supernatant for protein quantification was done using Pierce™ BCA protein assay kit (Thermo Fisher Scientific Inc.). Primary antibodies used ...
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bioRxiv - Cell Biology 2020Quote: ... 3 % SDS and 5 mM EDTA) and protein concentrations were determined with a Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was first quality-checked by gel electrophoresis and then quantified with a PicoGreen fluorometer quantitator and Quant-iT™ PicoGreen™ dsDNA Assay Kit (Invitrogen™, ThermoFisher Scientific). The Genomic Sequencing and Analysis Facility (GSAF ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of nucleic acid in the libraries was measured using a Quant-iT™ PicoGreen™ dsDNA Assay Kit (Invitrogen, Carlsbad, CA, USA) and 1.13 ng of each sample were pooled in 80 μl of nuclease-free water ...
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bioRxiv - Cell Biology 2021Quote: ... Flow SA-β-Gal staining assay was performed by flow cytometry using an ImaGene Green C12FDG lacZ gene expression kit from Molecular Probes (Life Technologies), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: The Click-iT™ Plus TUNEL Assay kit for in situ apoptosis detection with Alexa Fluor488 dyes was purchased from Invitrogen (Catalog Nos. C10617). The labeling protocol was as instructed by the manufacture’s manual.
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bioRxiv - Microbiology 2022Quote: ... the supernatant from the cell lysates were transferred to new tubes and protein concentration of the samples was determined using a Pierce BCA protein assay kit (Thermo Scientific, Waltham, MA, USA). The samples were then heated to 95°C for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... and PC-3 cells was performed by co-staining with a Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (Invitrogen, Cat. #C10425) and FxCycle Violet Ready Flow Reagent (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: EdU incorporation assay was performed following the instruction of Click-iT® Plus EdU Alexa Fluor® 594 Imaging Kit (C10639, Thermo Fisher Scientific). Growing cells on coverslips were incubated with 10 µM EdU for a designated time before the fixation with 4% PFA for 20 min ...
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bioRxiv - Genomics 2022Quote: ... Concentration and final size distribution of the libraries were evaluated using a Qubit™ 1X dsDNA HS Assay Kit (Thermo Fisher, Waltham, MA, USA) and Femto Pulse System (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of purified product was performed using Qubit™ dsDNA HS Assay kit with the use of a Qubit 2.0 fluorometer (Thermo Fisher Scientific, MA, USA). Sequencing libraries were prepared using the Nextera XT DNA Library kit (Illumina) ...
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bioRxiv - Microbiology 2022Quote: ... and the precise concentration was determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH, Darmstadt, Germany). Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina ...
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bioRxiv - Physiology 2022Quote: ... Supernatant was transferred to fresh tubes and the protein concentrations of the samples were measured with a Pierce BCA protein assay kit (Thermo Fisher Scientific Cat# 23227). Samples were prepared for electrophoresis by denaturing 20μg of protein per sample in XT Sample Buffer (Bio-Rad ...
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bioRxiv - Pathology 2022Quote: ... mice received 1.6 mg/kg EdU at 3 h before sacrifice and the staining was carried out according to the manufacturer’s instructions (Click-iT™ Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit, Thermo Fisher Scientific; c10340).
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bioRxiv - Pathology 2022Quote: ... liver was homogenized in RIPA buffer and protein levels were quantified using the Pierce™ BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA). Liver lysate and serum samples were analyzed by mass spectrometry for sphingolipid and fatty acid content at the metabolomics core at the Medical University of South Carolina ...
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bioRxiv - Physiology 2023Quote: ... Total protein was determined on the day of western blot experimentation and determined by using a bicinchoninic acid protein assay kit (ThermoFisher Scientific; Catalogue number: 23227) according to manufacturer’s protocol and measured on a Safire II (Tecan ...
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bioRxiv - Molecular Biology 2022Quote: ... was measured on two replicates per DNA extract with the Life Technologies (Carlsbad, California) Quant-iTTM PicoGreen® dsDNA assay kit using a microplate reader (Fluoroskan AscentTM FL; Thermo Scientific, Waltham, Massachusetts) and following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration of the supernatant for all protein samples was determined by Pierce BCA Protein Assay Kit (#23227, Thermo Scientific, Rockford, IL, USA).
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bioRxiv - Cancer Biology 2022Quote: ... 25 µl from prepared samples were added to one well of 96 well plate and Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) manual was followed to measure the protein content ...
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bioRxiv - Plant Biology 2024Quote: ... The quality and quantity of the total RNA were checked using a Qubit RNA HS Assay Kit and Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Total protein content in BAL fluid after cell removal was assessed using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Rockford, USA) according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2024Quote: ... and the concentration of total protein in cell extracts was determined using the Qubit® protein assay kit and the Qubit® 3.0 fluorometer (Thermo Fisher Scientific™). Cell culture supernatants were used purely ...
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bioRxiv - Systems Biology 2024Quote: ... Protein-containing supernatant was transferred to a new tube and protein concentrations was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Cat #23225) following the manufacturer protocol ...
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bioRxiv - Pathology 2024Quote: Total protein concentrations were assessed from all dialyzer membrane extracts using the Pierce™ BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA). The absorbance was taken at 562 nm on a microplate reader ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed in radioimmunoprecipitation assay-sodium dodecyl sulfate (RIPA-SDS) and protein concentration was quantified using a bicinchoninic acid (BCA) reagent kit (Thermo Fisher, Waltham, MA, USA) following manufacturer instructions ahead of western blotting.
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bioRxiv - Cell Biology 2024Quote: Flow cytometry-based detection of early and late apoptosis of cancer cells was conducted using the Annexin-V apoptosis assay kit (Thermofisher Scientific Cat. No. V13241). Staining of cells with Annexin-V and propidium iodide was conducted according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The differential level of nascent protein synthesis was quantified using Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (Invitrogen, Cat. # C10428) according to the manufacturer’s protocol on a BMG FLUOstar Omega Microplate Reader ...
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bioRxiv - Microbiology 2023Quote: ... and the precise concentration was determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH, Darmstadt, Germany). Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Protein-containing supernatant was transferred to a new tube and protein concentrations was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Cat #23225) following the manufacturer protocol.
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bioRxiv - Neuroscience 2024Quote: ... RNA quantity was measured using a Qubit 4 fluorometer (Qubit 4 fluorometer; Qubit dnDNA HS Assay Kit, Cat No. Q32854 Invitrogen, Eugene, OR, United States). RNA quality was assessed using an Agilent RNA Nano kit on a BioAnalyzer instrument (RNA 6000 Nano Kit ...
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bioRxiv - Genomics 2023Quote: ... 1 confluent well of a 6-well plate of HCT116 cells were harvested and processed as per manufacturer’s instructions for the Click-iT™ Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher cat: C10634). Per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were sonicated using a probe sonicator and protein concentration was quantified using the Pierce™ BCA Protein Assay Kit (Thermo Scientific™, 23227). Samples were prepared in 1X Laemmli buffer (2% SDS ...
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bioRxiv - Neuroscience 2023Quote: ... Dried residues were resuspended in MilliQ water and total protein content of each homogenate was measured with the Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA). 20 µl of each sample was injected into a 25 cm x 4.6 mm C18 Nucleosil column with 5-µm particles (Interchim ...
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bioRxiv - Genomics 2024Quote: ... Proliferation of cells in the last 18hr of stimulation was assessed by labelling and staining proliferating cells with Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific.) Edu positive cells were detected using a MACSQuant® Analyzer 10 Flow Cytometer (Miltenyi Biotec).
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bioRxiv - Genomics 2024Quote: ... The eluted sample was quantified by the Qubit® Fluorometer and Qubit™ dsDNA HS and BR Assay Kits (Invitrogen™ Cat. No. Q32851). In total ...
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bioRxiv - Cancer Biology 2024Quote: RNA concentration was measured using Qubit® RNA HS Assay Kit on a Qubit® 3.0 Fluorometer (Thermo Fisher Scientific Inc., Waltham, MA, USA). Integrity was assessed using Agilent RNA 6000 Pico Kit on a 2100 Bioanalyzer instrument (Agilent Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... cells scraped off and transferred to a 1.5 ml tube for cell lysis before clearance by centrifugation and protein concentration measurement using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) following manufactureŕs instructions and the FLUOstar Omega reader ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were treated with aptamers at reported concentrations or with vehicle (DPBS and Mg2+) for 48 h and then stained according to the CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit (ThermoFisher Scientific, cat: #C10427). Briefly ...
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bioRxiv - Biophysics 2024Quote: ... HPG labeling of proteins was detected using the Click-iT™ HPG Alexa Fluor™ 594 Protein Synthesis Assay Kit (Life Technologies, cat. #C10429), following the manufacturer’s protocol ...