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Citations for Thermo Fisher :
9051 - 9100 of 10000+ citations for Recombinant Human PDCD1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Supernatants (2 mg protein) were removed and incubated overnight at 4°C with Dynabeads Protein G (Thermo Scientific) coated with anti-FLAG antibody or IgG heavy chain according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein fractions were quantified using an 8-point Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, PI23225), and 20 µg of protein per sample diluted to 35µL using NuPAGE LDS sample buffer (1x final conc. ...
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bioRxiv - Molecular Biology 2024Quote: ... protein concentrations of sheared chromatin were quantified with the Pierce™ BCA Protein Assay Kit (Thermo Scientific, #23227) following manufacturer instructions.
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bioRxiv - Bioengineering 2024Quote: ... Total protein concentration was quantified by Micro BCA Protein Assay Kit according to the manufacturer’s instructions (Thermo Scientific). Samples and migration standards were prepared under reducing conditions and incubated for 10 min at 37 °C prior to loading ...
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bioRxiv - Physiology 2024Quote: ... The protein concentration in the supernatant was then determined using the BCA Protein Assay Kit (Thermo Fisher Scientific). For protein analysis in isolated mitochondria ...
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bioRxiv - Developmental Biology 2024Quote: ... The supernatant was kept it in ice and protein concentration were measured with QUBIT protein kit (#Q33211, ThermoFisher).
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bioRxiv - Developmental Biology 2024Quote: ... The amount of protein in the lysate was quantified by NanoOrange™ Protein Quantitation Kit (Thermo Fisher Scientific) and the enzymatic activity of glutamine synthetase was measured by Glutamine Synthetase Activity Assay Kit (Abcam) ...
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bioRxiv - Developmental Biology 2024Quote: ... The lysate protein content was quantified with the Pierce™ BCA Protein Assay Kits (ThermoFisher Scientific, Cat.#: 23225) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated by SDS-PAGE on NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gels (Invitrogen) in MOPS running buffer ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... The EV protein concentration was measured by using a Micro BCA Protein Assay Kit (23235, Thermo Fisher Scientific). The collection method ...
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bioRxiv - Cancer Biology 2024Quote: ... and estimated the protein concentrations using Pierce BCA Protein Assay Kit as per the manufacturer’s protocol (ThermoFisher Scientific). Around 10 µg of proteins were allowed to resolve in 8% SDS-PAGE separating gel ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, cat. no. 23225). The concentration of samples was adjusted by dilution with RIPA buffer ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then incubated in biotinylated donkey anti-rabbit IgG (1:100; Amersham, Buckinghamshire, United Kingdom) and streptavidin-conjugated Alexa fluor 488 (1:1000; Molecular Probes, Eugene, OR, United States of America). Finally ...
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bioRxiv - Biochemistry 2021Quote: Recombinant baculovirus carrying hXkr8-TH-mGFP or hBSGΔ cDNA was obtained with the Bac-to-Bac Baculovirus Expression System (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: Cryopreserved stocks of the KH004 parent and the Mal31×KH004 uncloned bulk recombinant progeny pool and were thawed and grown in complete media (CM) [RPMI 1640 with L-glutamine (Gibco, Life Technologies), 50 mg/L hypoxanthine (Calbiochem ...
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bioRxiv - Biochemistry 2022Quote: ... Rhodopsin expressed at the plasma membrane of recombinant cells was labeled with a DyLight 550–conjugated anti-HA antibody (ThermoFisher, Waltham, MA). Labelled cells were then fractionated into quartiles according to surface immunostaining intensity using a FACS Aria IIu fluorescence activated cell sorter (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... purified CD4+ T cells were maintained for two days in complete RPMI 1640 medium containing recombinant interleukin-2 (IL-2) (300 IU/ml) and phytohemagglutinin (1 μg/ml, Thermo Fisher Scientific), followed by 3 days in the presence of IL-2 alone ...
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bioRxiv - Bioengineering 2021Quote: ... Qiagen miniprep Kit`for recombinant plasmid isolation and purification and SDS-NuPAGE precast gels for molecular characterization of expressed enzyme were obtained from Invitrogen (Carlsbad, CA). Polygalacturonic acid (PGA ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant bacmids were selected by plating on Luria-Bertani (LB) agar plates with 50 μg/ml kanamycin (Gibco from Thermo Fisher Scientific) and 10 μg/ml tetracycline (MilliporeSigma ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant bacmids were selected by plating on Luria-Bertani (LB) agar plates with 50 μg/ml kanamycin (Gibco from Thermo Fisher Scientific) and 10 μg/ml tetracycline (MilliporeSigma ...
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bioRxiv - Microbiology 2021Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Cells were stained with a polyclonal antibody raised in rabbit against purified recombinant BT1636 (BT1636Ab, Cocalico Biologicals) and detected with an Alexa Fluor® 488-conjugated goat anti-rabbit IgG secondary antibody (Molecular Probes). Images were taken with Zeiss Apotome using the same exposure time between samples.
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bioRxiv - Immunology 2019Quote: ... In some groups culture media was supplemented with recombinant osteopontin (2 μg/ml) (R&D) or anti-CD44 (5 μg/ml) (Thermofisher; clone IM7). Cells were cultured in 5% CO2 at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... CFTR expressed at the plasma membrane of recombinant cells was labeled with a DyLight 550–conjugated anti-HA antibody (ThermoFisher, Waltham, MA). Labelled cells were then fractionated into quartiles according to surface immunostaining intensity using a FACS Aria IIu fluorescence activated cell sorter (BD Biosciences ...
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bioRxiv - Biochemistry 2022Quote: ... 12 pmol crRNA:tracrRNA duplex was combined with 12 pmol recombinant HiFi Cas9 (IDT) and reverse transfected into HEK293 Flp-In cells using Lipofectamine CRISPRMAX reagent (Thermo Fisher Scientific) following IDT protocols ...
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bioRxiv - Cell Biology 2022Quote: ... Synthego, Redwood City, CA, USA), recombinant 2NLS-Cas9 nuclease (20 μM, Synthego, Redwood City, CA, USA) and optiMEM (Invitrogen, Waltham, MA, USA) supplemented with Y27632 (10 μM) ...
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bioRxiv - Molecular Biology 2022Quote: ... was mixed with 2 µl of recombinant yeast Poly(A) Polymerase (PAP) together with 5 µl of 5X PAP buffer (Thermo Fisher Scientific), 2.5 µl of 10 µM GTP/ITP/MgCl2 (Jena Bioscience) ...
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bioRxiv - Microbiology 2023Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and 33 µl of this buffer was added to the beads together with 10 µl Recombinant Proteinase K Solution (20 mg/ml, Thermo Fisher, AM2548) and 3 µl RiboLock RNase inhibitor (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... Fragments were end-repaired with Enzymatics low concentration end repair mix (Y9140-LC-L) and A-tailed with Recombinant Taq (Life Technologies, 100021276). Y-Adapters were pre-annealed by incubating a MiSeq Common Adapter with Adapters (A01-A10 ...
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bioRxiv - Biochemistry 2023Quote: Insect cell derived recombinant tag-less eIF2A was expressed in ExpiSf9 cells using the ExpiSf Expression System Starter Kit (Thermo Fisher # A38841) following the manufacturer’s protocol and adapting the IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag ...
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bioRxiv - Microbiology 2023Quote: ... The sequences of all four recombinant viruses generated in this study were confirmed by sequencing with a SeqStudio Genetic Analyzer (Thermo Fisher Scientific) and an outsourced service (Fasmac).
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bioRxiv - Biochemistry 2022Quote: Recombinant hItln1 and MBL were characterized by western blotting using monoclonal mouse anti-MBL2 antibody (3E7; ThermoFisher Scientific MA1-40145; 1:50) and polyclonal rabbit anti-hItln1 antibody (Proteintech 11770-1-AP ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were centrifuged at 1400 rpm for 4 minutes and incubated with Ki-67 Recombinant Rabbit Monoclonal Antibody (SP6) (ThermoFisher, MA5-14520) at room temperature for 30 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... both diluted 1:50 in DMEM/F-12 or with 20 ug/mL recombinant truncated vitronectin (VTN-N) (Thermo Fischer Scientific, A31804) diluted in DMEM/F-12 (Gibco, 21331-020) for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Single colonies were picked and cultured overnight in LB medium + 50 µg/mL kanamycin and recombinant plasmids isolated using the GeneJET Plasmid Miniprep Kit (Thermo Fisher #K0503). Insert sequences in each plasmid were confirmed by Sanger sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 construct was tagged on the N-terminus with 10xHis and MBP tag cleavable by TEV protease ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 constructs were tagged on the N-terminus with 10xHis and MBP tag cleavable by TEV protease ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 medulloblastoma mutations were introduced into coding regions using standard PCR-based site-directed mutagenesis techniques ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA for human HDAC1 with C-terminal FLAG tag was cloned into the pFastBac donor vector using standard PCR-based site-directed mutagenesis techniques and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). Detection of gp64 was used to determine baculovirus titer (Expression Systems) ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 protein was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 construct was tagged on the N-terminus with 6×His cleavable by TEV protease ...
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bioRxiv - Cancer Biology 2021Quote: ... respectively coupled with protein G Dynabeads (Invitrogen) with nuclear extract derived from MCF-7 cells for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... Dynabeads Protein A or G (Life Technologies), 25 µl per IP ...
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bioRxiv - Bioengineering 2021Quote: ... Dynabeads M-280 Protein G (ThermoFisher, 10003D) were resuspended by vortexing for 30 s ...
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bioRxiv - Biophysics 2020Quote: ... B-PER Bacterial Protein Extraction Reagent (ThermoFisher) was used to lyse the cells in the presence of protease inhibitor ...
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bioRxiv - Cancer Biology 2021Quote: ... or DynabeadsTM protein G beads (#10004D, Invitrogen) were added into each sample and incubated at 4 °C for 1-2 hours on the next day ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein G Dynabeads (Thermo Fisher Scientific, #10003D), protein G beads (EMD Millipore ...
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bioRxiv - Cancer Biology 2021Quote: ... Sypro Ruby Protein Blot Stain (Molecular Probes) was used to quantify protein concentration in each sample ...
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bioRxiv - Cell Biology 2020Quote: ... Dynabead Protein G beads (ThermoFisher, Waltham MA) were added to a concentration of 30 µL/mL and rotated for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2020Quote: 100 µl of Dynabeads Protein A (ThermoFisher) were prepared for immunoprecipitation (IP ...