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Citations for Thermo Fisher :
8901 - 8950 of 10000+ citations for Recombinant Human PDCD1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentrations were measured using the BCA Protein Assay kit according to the manufacturer’s instructions (Thermo Scientific Pierce). Whole protein (30μg/lane ...
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bioRxiv - Cancer Biology 2022Quote: ... Beads were prepared by incubating them in 0.5% BSA in PBS and antibodies overnight (100μL of Dynabeads Protein A or Protein G (Invitrogen) plus 20μL of antibody) ...
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bioRxiv - Developmental Biology 2022Quote: Global novel protein synthesis was assessed using the Click-iT Plus OPP Protein Synthesis Assay Kit (Life Technologies, #C10456 for green fluorescence ...
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bioRxiv - Developmental Biology 2022Quote: ... The antibody-DNA complex was precipitated with protein A/G magnetic beads or protein A sepharose beads (Invitrogen). After washing ...
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bioRxiv - Cell Biology 2022Quote: The protein concentration of each fraction was measured using the Pierce BCA Protein Assay Kit (#23227, Thermo Scientific™ ...
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bioRxiv - Cancer Biology 2022Quote: ... Total concentration of proteins in cell lysates was measured by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the recommendations from the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration of the cleared lysate was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific). The CatchPoint cAMP ELISA was then performed according to the manual using 0.8 µg protein as input ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration in the supernatant was determined using Pierce 660 nm protein assay (Thermo Fisher Scientific, 22660) and the amounts of protein (~0.5 μg ...
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bioRxiv - Genomics 2022Quote: ... Protein concentration in each fraction extract was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Following separation on precast SDS-PAGE gels (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... Total protein concentration was assessed using the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA), and toxin levels reported as OD450nm/mg total protein ...
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bioRxiv - Microbiology 2022Quote: ... Protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific, cat. no. 23225, Rockford, IL).
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bioRxiv - Microbiology 2022Quote: Protein concentrations were determined using a Pierce™ BCA Protein Assay kit (Thermo Fisher Scientific, Waltham, MA, USA) and a Benchmark Plus Microplate Reader (Bio-Rad ...
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bioRxiv - Pathology 2022Quote: Protein concentration of all cellular lysates were first determined using the Pierce BCA Protein Assay Kit #PI23227 (ThermoFisher) prior to SDS-PAGE ...
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bioRxiv - Molecular Biology 2023Quote: Total proteins were harvested from tissue and cell lines lysates using M-PER protein extraction reagent (Thermo Fisher) respectively supplemented with proteinase and phosphatase inhibitors (Sigma ...
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bioRxiv - Plant Biology 2023Quote: ... The amount of protein was determined with the PierceTM BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA).
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bioRxiv - Neuroscience 2023Quote: ... Relative protein amount for each sample was determined using the Micro BCA Protein Assay Kit (ThermoFisher; Cat# 23235), and the ELISA readouts were normalized to account for the differences in total protein loaded into each well ...
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bioRxiv - Microbiology 2024Quote: ... and the protein concentration was determined using Pierce™ BCA Protein Assay Kits (Thermo Fisher Scientific, cat# 23225). The lysates were subsequently reduced with 1 × Protein Loading buffer (TransGen ...
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration was measured by Pierce BCA Protein Assay kit (Thermo Fisher Scientific, Rock ford, IL, USA). The peptides mixtures were clean-up to obtain detergent-free samples with the same kit ...
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bioRxiv - Synthetic Biology 2022Quote: ... The protein concentration was measured using the Qubit Protein Assay Kit and Qubit 3 Fluorometer (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... Total protein amounts per lane were stained with No-stain™ Protein Labelling Reagent (Thermo Fisher Scientific; A44449) and used for lane or dot normalization ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was collected and protein concentration was determined by the Pierce BCA protein assay kit (ThermoFisher, 23225). 250μg of each cell lysate were reduced with 5 mM DTT for 1 h at 37 °C and alkylated with 10 mM iodoacetamide for 30 min at room temperature in the dark ...
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bioRxiv - Microbiology 2022Quote: ... Protein precipitate was solubilized in 8M urea and Bicinchoninic (BCA) protein assay kit (Thermo Fisher Scientific, MA, USA) was used to determine the protein concentrations of each sample.
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bioRxiv - Neuroscience 2022Quote: ... brain lysates were assayed for total protein concentration using Pierce BCA protein assay (Thermo Fisher Scientific, Rockford, Illinois) as per manufacturer protocol (50-fold dilution) ...
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bioRxiv - Bioengineering 2022Quote: ... Sample protein concentration was measuring using a Bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, MA, USA), with bovine serum albumin standards ranging from 20-2000 μg/mL.
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bioRxiv - Cell Biology 2024Quote: ... and the protein concentration measured using the Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA). Samples were diluted to 1.2 µg/µl with RIPA buffer and mixed with the respective amount of 6x Laemmli buffer (375 mM Tris pH 6.8 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein Thermal Shift dye and Tris buffer according to the Protein Thermal Shift™ Dye Kit (ThermoFisher Scientific) protocols ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were determined from cleared lysates with either the PierceTM BCA Protein Assay Kit (Thermo Scientific #23225) or the PierceTM Rapid Gold BCA Protein Assay Kit (Thermo Scientific #A53225PR) ...
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bioRxiv - Biophysics 2024Quote: ... Protein samples were separated using pre-cast 4-12% Bis Tris Protein Gels (Thermo-Fisher Scientific, Waltham MA) for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... Resulting protein concentrations were determined with a Bicinchonic acid assay (BCA - Pierce BCA Protein Assay Kit, ThermoFisher, Germany) and 20ug of protein were filled up to 140ul with 100mM HEPES pH 8 and transferred to a 96 deep-well plate ...
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bioRxiv - Cancer Biology 2024Quote: ... pierce BCA protein assay kit (Cat# 23227) and page ruler protein ladder (Cat# 26620) were purchased from Thermofisher.
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations of the samples were determined with a standard BCA protein assay (Thermo Fisher Scientific, Waltham, USA) and 4 µg of each sample was pipetted into a final volume of 50 µl in AMBIC for the tryptic peptide preparation ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration of total extracts was measured by the BCA Protein Assay Kit (Thermo Scientific™, #23225). The same mass of total extracts was taken for a high spin at 154,000 g for 20 minutes in 4 °C using an ultracentrifuge (Beckman ...
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bioRxiv - Neuroscience 2024Quote: ... Total protein concentration for each sample was measured using a BCA Protein Assay kit (cat # 23225, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... total protein loading on the PVDF membrane was stained with No-StainTM Protein Labeling reagent (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Antibodies were purified from culture supernatants by protein A agarose beads (Thermo Fisher PierceTM Protein A Agarose, 20333). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... Total protein concentrations of lysates were determined using the BCA protein assay kit (Thermo Fisher Scientific; Catalog #23225). 70µg of total protein was loaded and resolved on 6 or 10% SDS-PAGE gels ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was measured from the supernatant using the PierceTM BCA Protein Assay Kit (ThermoFisher Scientific, Cat # 23225).
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bioRxiv - Microbiology 2024Quote: The quantification of total protein concentration was performed using the Pierce BCA Protein Assay Kit from (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Lysates were then then quantified for total protein content using the PierceTM BCA Protein Assay Kit (ThermoFisher Scientific) then either snap frozen in liquid nitrogen and stored at -80 °C or immediately used in downstream experiments.
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bioRxiv - Microbiology 2024Quote: ... Total protein content was quantified using the Pierce™ BCA Protein Assay Kit™ (ThermoFisher Scientific, Waltham, MA) and 10 µg of protein was diluted into 25 µL of 1x S-TRAP buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentrations in the supernatants were measured using the EZQ® Protein Quantitation Kit (Molecular Probes, Inc.) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... and protein determination was performed using the Bradford colorimetric assay with BSA as the standard protein (Thermo Scientific). Absorbance was measured at a wavelength of 595nm using a hybrid microplate reader (Biotek).
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bioRxiv - Cancer Biology 2023Quote: ... the protein amount of the supernatant was quantified using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) and Synergy LX Multimode Reader (Agilent) ...
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bioRxiv - Systems Biology 2023Quote: ... Protein concentrations in the pellet were measured with the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). The collected supernatant (180 μL ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration in each faction was determined by a BCA protein assay kit (Thermo Fisher Scientific, PI23227). Immunoblots were performed as described previously (Farooq et al. ...
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bioRxiv - Cell Biology 2023Quote: Newly synthetized proteins were labelled using the Click-iT HPG Alexa Fluor488 Protein Synthesis Assay Kit (Thermo Fisher). Neurons were incubated with 0.1 mM L- homopropargylglycine (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... The total protein yield was determined by Pierce Bradford assay (Pierce™ BCA Protein Assay Kit, ThermoFisher Scientific). Proteins were then transferred to membranes and immunoblotting was performed using the antibodies described in Supplementary Table 2.
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bioRxiv - Cell Biology 2023Quote: Total protein concentrations were measured in cell lysates using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). A total of 15 µg of total protein fraction for each sample was separated by electrophoresis using NuPAGE™ 4-12% Bis-Tris Protein Gel (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Contrast-to-mass calibration was carried out using a mixture of native proteins (NativeMark Unstained Protein Standard, Invitrogen) with molecular weight (MW ...
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bioRxiv - Cell Biology 2023Quote: ... Prostaglandin E2 level was measured and standardized with protein level using BCA Protein Assay Kit (Thermo Fisher, #23227).