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Citations for Thermo Fisher :
9051 - 9100 of 10000+ citations for QuantiChrom Indole Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: EdU incorporation assay was performed following the instruction of Click-iT® Plus EdU Alexa Fluor® 594 Imaging Kit (C10639, Thermo Fisher Scientific). Growing cells on coverslips were incubated with 10 µM EdU for a designated time before the fixation with 4% PFA for 20 min ...
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bioRxiv - Genomics 2022Quote: ... Concentration and final size distribution of the libraries were evaluated using a Qubit™ 1X dsDNA HS Assay Kit (Thermo Fisher, Waltham, MA, USA) and Femto Pulse System (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of purified product was performed using Qubit™ dsDNA HS Assay kit with the use of a Qubit 2.0 fluorometer (Thermo Fisher Scientific, MA, USA). Sequencing libraries were prepared using the Nextera XT DNA Library kit (Illumina) ...
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bioRxiv - Microbiology 2022Quote: ... and the precise concentration was determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH, Darmstadt, Germany). Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina ...
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bioRxiv - Physiology 2022Quote: ... Supernatant was transferred to fresh tubes and the protein concentrations of the samples were measured with a Pierce BCA protein assay kit (Thermo Fisher Scientific Cat# 23227). Samples were prepared for electrophoresis by denaturing 20μg of protein per sample in XT Sample Buffer (Bio-Rad ...
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bioRxiv - Pathology 2022Quote: ... mice received 1.6 mg/kg EdU at 3 h before sacrifice and the staining was carried out according to the manufacturer’s instructions (Click-iT™ Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit, Thermo Fisher Scientific; c10340).
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bioRxiv - Pathology 2022Quote: ... liver was homogenized in RIPA buffer and protein levels were quantified using the Pierce™ BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA). Liver lysate and serum samples were analyzed by mass spectrometry for sphingolipid and fatty acid content at the metabolomics core at the Medical University of South Carolina ...
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bioRxiv - Physiology 2023Quote: ... Total protein was determined on the day of western blot experimentation and determined by using a bicinchoninic acid protein assay kit (ThermoFisher Scientific; Catalogue number: 23227) according to manufacturer’s protocol and measured on a Safire II (Tecan ...
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bioRxiv - Molecular Biology 2022Quote: ... was measured on two replicates per DNA extract with the Life Technologies (Carlsbad, California) Quant-iTTM PicoGreen® dsDNA assay kit using a microplate reader (Fluoroskan AscentTM FL; Thermo Scientific, Waltham, Massachusetts) and following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration of the supernatant for all protein samples was determined by Pierce BCA Protein Assay Kit (#23227, Thermo Scientific, Rockford, IL, USA).
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bioRxiv - Cancer Biology 2022Quote: ... 25 µl from prepared samples were added to one well of 96 well plate and Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) manual was followed to measure the protein content ...
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bioRxiv - Plant Biology 2024Quote: ... The quality and quantity of the total RNA were checked using a Qubit RNA HS Assay Kit and Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Total protein content in BAL fluid after cell removal was assessed using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Rockford, USA) according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2024Quote: ... and the concentration of total protein in cell extracts was determined using the Qubit® protein assay kit and the Qubit® 3.0 fluorometer (Thermo Fisher Scientific™). Cell culture supernatants were used purely ...
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bioRxiv - Systems Biology 2024Quote: ... Protein-containing supernatant was transferred to a new tube and protein concentrations was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Cat #23225) following the manufacturer protocol ...
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bioRxiv - Pathology 2024Quote: Total protein concentrations were assessed from all dialyzer membrane extracts using the Pierce™ BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA). The absorbance was taken at 562 nm on a microplate reader ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed in radioimmunoprecipitation assay-sodium dodecyl sulfate (RIPA-SDS) and protein concentration was quantified using a bicinchoninic acid (BCA) reagent kit (Thermo Fisher, Waltham, MA, USA) following manufacturer instructions ahead of western blotting.
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bioRxiv - Cell Biology 2024Quote: Flow cytometry-based detection of early and late apoptosis of cancer cells was conducted using the Annexin-V apoptosis assay kit (Thermofisher Scientific Cat. No. V13241). Staining of cells with Annexin-V and propidium iodide was conducted according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The differential level of nascent protein synthesis was quantified using Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (Invitrogen, Cat. # C10428) according to the manufacturer’s protocol on a BMG FLUOstar Omega Microplate Reader ...
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bioRxiv - Microbiology 2023Quote: ... and the precise concentration was determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH, Darmstadt, Germany). Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Protein-containing supernatant was transferred to a new tube and protein concentrations was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Cat #23225) following the manufacturer protocol.
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bioRxiv - Neuroscience 2024Quote: ... RNA quantity was measured using a Qubit 4 fluorometer (Qubit 4 fluorometer; Qubit dnDNA HS Assay Kit, Cat No. Q32854 Invitrogen, Eugene, OR, United States). RNA quality was assessed using an Agilent RNA Nano kit on a BioAnalyzer instrument (RNA 6000 Nano Kit ...
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bioRxiv - Genomics 2023Quote: ... 1 confluent well of a 6-well plate of HCT116 cells were harvested and processed as per manufacturer’s instructions for the Click-iT™ Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher cat: C10634). Per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were sonicated using a probe sonicator and protein concentration was quantified using the Pierce™ BCA Protein Assay Kit (Thermo Scientific™, 23227). Samples were prepared in 1X Laemmli buffer (2% SDS ...
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bioRxiv - Neuroscience 2023Quote: ... Dried residues were resuspended in MilliQ water and total protein content of each homogenate was measured with the Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA). 20 µl of each sample was injected into a 25 cm x 4.6 mm C18 Nucleosil column with 5-µm particles (Interchim ...
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bioRxiv - Genomics 2024Quote: ... Proliferation of cells in the last 18hr of stimulation was assessed by labelling and staining proliferating cells with Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific.) Edu positive cells were detected using a MACSQuant® Analyzer 10 Flow Cytometer (Miltenyi Biotec).
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bioRxiv - Genomics 2024Quote: ... The eluted sample was quantified by the Qubit® Fluorometer and Qubit™ dsDNA HS and BR Assay Kits (Invitrogen™ Cat. No. Q32851). In total ...
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bioRxiv - Cancer Biology 2024Quote: RNA concentration was measured using Qubit® RNA HS Assay Kit on a Qubit® 3.0 Fluorometer (Thermo Fisher Scientific Inc., Waltham, MA, USA). Integrity was assessed using Agilent RNA 6000 Pico Kit on a 2100 Bioanalyzer instrument (Agilent Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... cells scraped off and transferred to a 1.5 ml tube for cell lysis before clearance by centrifugation and protein concentration measurement using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) following manufactureŕs instructions and the FLUOstar Omega reader ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were treated with aptamers at reported concentrations or with vehicle (DPBS and Mg2+) for 48 h and then stained according to the CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit (ThermoFisher Scientific, cat: #C10427). Briefly ...
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bioRxiv - Biophysics 2024Quote: ... HPG labeling of proteins was detected using the Click-iT™ HPG Alexa Fluor™ 594 Protein Synthesis Assay Kit (Life Technologies, cat. #C10429), following the manufacturer’s protocol ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... The purified SEP was passed through a 0.2 µm pore size syringe filter for sterilization and quantified using the BCA Protein Assay kit (Thermo Fisher Scientific, Les Ulis, France). Aqueous solutions were stored at -20°C until further use.
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bioRxiv - Cancer Biology 2023Quote: Cell death was assessed using the Click-iT™ Plus TUNEL Assay for In Situ Apoptosis Detection Alexa Fluor™ 647 Imaging Kit (Life Technologies, C10617). Unless otherwise stated ...
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bioRxiv - Microbiology 2022Quote: ... the resulting supernatant of each sample was transferred to a labeled microcentrifuge tube and stored at -20°C The Quant-iT dsDNA Assay Kit (Thermo Fisher Scientific, MA, USA) was used to measure DNA concentrations prior to PCR amplification ...
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bioRxiv - Plant Biology 2023Quote: ... Final libraries were assessed for size and quantity using an Agilent DNA1000 chip and a Qubit® dsDNA HS Assay Kit (Invitrogen, Carlsbad, California, USA), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 42 commercial antibodies (see Table S2 for catalogue numbers and dilution detail) were buffer-exchanged with PBS and quantified using the Pierce® BCA Protein Assay Kit (23227, Thermo Scientific, Waltham, US). Approximately 1 nl was printed per feature on Nexterion H amine-reactive hydrogel-coated glass slides using a SciFLEXARRAYER S3 piezoelectric printer (Scienion ...
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bioRxiv - Genomics 2022Quote: ... EdU staining was performed on thawed nuclei using Click-iT Plus EdU Alexa Fluor™ 647 Flow Cytometry assay Kit (Thermo Fisher Scientific, 10634). A 500 µL reaction buffer (prepared following the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... EdU staining was performed on thawed nuclei using Click-iT Plus EdU Alexa Fluor™ 647 Flow Cytometry assay Kit (Thermo Fisher Scientific, 10634). A 500 μL reaction buffer (prepared following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and ventral hippocampus were quantified using a Pierce Microplate BCA Protein Assay Kit (Thermo Scientific Pierce Protein Biology, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... mice were sacrificed 24 h post exposure to Cl2 or air and lavaged as described previously(7, 8) Total protein concentrations in cell-free lavage were measured with the BCA Protein Assay Kit (#23225; Thermo Fisher Scientific, Rockford, IL). Cells were re-suspended in 100μl of phosphate buffered saline (PBS ...
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bioRxiv - Physiology 2023Quote: ... The supernatant (islet sample) was collected and the total protein was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... DNA concentration was assessed by fluorometry using a Qubit™ 4 and Qubit™ 1X dsDNA HS Assay Kit (ThermoFisher Scientific, Waltham, MA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Labeling and detection of apoptotic cells were completed using the Invitrogen Click-iT Plus TUNEL Assay for In Situ Apoptosis Detection 488 kit (Invitrogen, Waltham, MA, catalog #: C10617) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell cycle analysis was performed by flow cytometry and in the final hour of drug treatment 10 μM EdU was added to the media containing drug to accurately identify cells in S-phase using a Click-iT™ EdU flow cytometry assay kit (Thermofisher, Waltham, MA, USA). Cells were harvested and fixed by 4% paraformaldehyde (15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein pellets were subsequently re-solubilized in 40 uL of 5% SDS (v/v) and assayed for protein using the PierceTM BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA). 130-170ug of protein/sample (in a total volume of 33 uL) ...
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bioRxiv - Microbiology 2023Quote: ... DNA concentrations of all PCR products were measured using a Qubit® Flex Fluorometer with the Qubit dsDNA BR Assay Kit (Invitrogen, Waltham, MA, USA) and normalised to 2 ng/μL ...
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bioRxiv - Biochemistry 2022Quote: ... were centrifuged at 16,000 g for 30 minutes at 4 °C and the supernatant was collected for protein estimation using Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific; Cat# 23227). Equal amounts of liver protein (100 μg ...
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bioRxiv - Cell Biology 2022Quote: ... The intensity values were normalized by the total protein in each sample (determined by the Pierce™ BCA Protein assay kit, ThermoFisher Scientific, cat# 23225) before they were used for quantitative analysis.
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bioRxiv - Cancer Biology 2023Quote: β-galactosidase activity in the PB3 and MDA-MB-231 cells after different treatments were evaluated using the CellEvent™ Senescence Green Flow Cytometry Assay Kit (Thermo Fisher Scientific #C10841) and manufacturer’s protocols ...
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bioRxiv - Bioengineering 2023Quote: Total miR levels in extracted RNA samples were quantified using a Qubit miR Assay Kit and Qubit 3.0 Fluorimeter (Thermo Fisher Scientific, Waltham, MA, USA); the manufacturer’s protocols were employed.