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Citations for Thermo Fisher :
8951 - 9000 of 10000+ citations for QuantiChrom Indole Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: The micro-BCA assay (ThermoFisher, Ca#23235) is used to quantify the amount of protein in our MSC-EV batch ...
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bioRxiv - Cancer Biology 2024Quote: ... St6Gal1 (Thermo Fisher Scientific Assay ID: Mm00486119_m1); St6GalNac3 (Thermo Fisher Scientific Assay ID ...
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bioRxiv - Cancer Biology 2024Quote: ... St6GalNac3 (Thermo Fisher Scientific Assay ID: Mm01316813_m1); and RPS17(Thermo Fisher Scientific Assay ID ...
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bioRxiv - Neuroscience 2024Quote: ... bicinchoninic acid assay (BCA, 23225, Thermo Scientific) and a plate reader (Infinite® M200 ...
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bioRxiv - Molecular Biology 2024Quote: ... and TaqMan Gene Expression Assays (Applied Biosystems) or SYBR Green custom oligonucleotides (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... CyQUANT LDH Cytotoxicity Assay was from Invitrogen. Anti-CD11b (M1-70) ...
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bioRxiv - Physiology 2024Quote: ... Taqman gene expression assays from Thermo Fisher Scientieic (Waltham ...
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bioRxiv - Microbiology 2024Quote: ... and TaqMan Gene Expression Assays (Applied Biosystems) for Gapdh (HS02786624_g1) ...
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bioRxiv - Developmental Biology 2024Quote: LanthaScreen TR-FRET Kinase Assay (ThermoFisher Scientific) was used according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... a BCA assay (Thermo Fisher Scientific #23225) was performed ...
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bioRxiv - Microbiology 2020Quote: ... DNA was quantified using a Qubit double-stranded broad range DNA Assay or the high-sensitivity assay (ThermoFisher Scientific) if necessary ...
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bioRxiv - Developmental Biology 2022Quote: ... ETC complex activity assays were normalized to cell lysate protein content via Bicinchoninic Acid (BCA) assay (Pierce, ThermoFisher Scientific) as per manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... Cells were then lysed and a caspase-3 assay was performed according to the manufacturer’s protocol (EnzChek caspase-3 Assay, Invitrogen). The intensity of fluorescence was analyzed using an EnVizion plate reader.
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bioRxiv - Neuroscience 2020Quote: ... a luciferase glow assay was performed according to manufacturer’s instructions (Thermo Scientific Pierce Firefly Glow Assay; Thermo Scientific 16177). Briefly ...
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bioRxiv - Molecular Biology 2019Quote: The Click-iT EdU assay (which is similar to the BrdU assay) was performed according to the manufacturer’s (Invitrogen) instructions ...
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bioRxiv - Genetics 2019Quote: ... SNPs that could not be genotyped using OpenArray assays were genotyped individually using TaqMan SNP Genotyping assays (Applied Biosystems). Eight variants could not be genotyped due to technical difficulties.
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bioRxiv - Cancer Biology 2020Quote: Kinase assays were performed using the Omnia Kinase Assay according to the manufacturer’s protocol (Life Technologies, Carlsbad, CA, USA); see Supplemental Methods.
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bioRxiv - Physiology 2019Quote: ... Each 6 μL assay master mix (AMM) consisted of 3 μL of TaqMan® Gene Expression assay 20X (ThermoFisher) and 3 μL of 2X Assay Loading Reagent (Fluidigm) ...
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bioRxiv - Pathology 2020Quote: ... Assay ID 245306_mat; 4427975, hsa-mir-23a-3p, Assay ID 000399; 4427975, cel-miR-39-3p, Assay ID 000200, Thermofisher). The relative expression of individual miRNA was calculated as ΔCt = mean Ct miRNA-mean Ct miR-39.
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bioRxiv - Microbiology 2021Quote: ... DNA was quantified using a Qubit double-stranded broad range DNA Assay or the high-sensitivity assay (ThermoFisher Scientific) if necessary ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time PCR (qPCR) was performed using the TaqMan® Gene Expression UGCG Assay ID:Hs00916612_m1 and SPTLC3 Assay ID:Hs00217867_m1 (Thermo Fisher Scientific) or KAPA™ probe fast qPCR Master Mix (Kapa Biosystems ...
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bioRxiv - Neuroscience 2022Quote: For medium throughput assays performed in ASO screens and IC50 studies we used the Cells-to-Ct assays (ThermoFisher). These assays were combined with Taqman qPCR kits for human STAU1 (ThermoFisher Hs00244999_m1 ...
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bioRxiv - Physiology 2022Quote: ... Total protein in an average 11 µL supernatant was estimated by the Pierce BCA assay following the manufacturer’s protocol for microplate assay (Thermofisher). An average of 6.1 µg (S.D ...
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bioRxiv - Neuroscience 2023Quote: Quantitative PCR on cDNA samples was performed using FAM-labeled C1ql1 (Taqman Gene Expression Assay, ThermoFisher, Assay ID: Mm00657289_m1) or Cre (Taqman Gene Expression Assay ...
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bioRxiv - Genomics 2022Quote: ... RNA quality was checked using a Bioanalyzer RNA 6000 nano chip assay and Ribogreen assay (Invitrogen, Carlsbad, CA, USA). 437ng RNA per sample was used for further analysis ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected and the protein concentration was quantified using bicinchoninic acid protein assay (BCA assay; Pierce, Thermo Scientific). 50 μg of protein lysate was denatured and were run on a 4-12% SDS-PAGE gel ...
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bioRxiv - Cell Biology 2023Quote: ... chicken STEAP4 metalloreductase (STEAP4, assay ID number Gg07188578_m1) and the reference gene chicken GAPDH (assay ID number Gg03346982_m1) (Thermofisher Scientific). The PCR cycle parameters were an initial denaturing step at 95 °C for 10 min followed by 45 cycles of 95 °C for 15 s and 60 °C for 1 min ...
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bioRxiv - Neuroscience 2023Quote: ... we used 1 µg total RNA to generate cDNA followed by Taqman qPCR using Taqman Universal Master Mix and Taqman probes for Nsun2 (Assay ID: Mm01349532_m1) and Gapdh as a control gene (Assay ID: Mm99999915_g1; Applied Biosystems). For qPCR to detect change in tRNA expression ...
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bioRxiv - Microbiology 2024Quote: ... The concentrations of the protein extracts were determined using a BCA assay (Pierce BCA Protein Assay, Thermo Fisher Scientific) and a verification of the protein extracts was carried out on a 12.5% acrylamide SDS-PAGE gel stained with Coomassie brilliant blue R-250 ...
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bioRxiv - Plant Biology 2019Quote: ... dsDNA concentration was measured by Qubit® 2.0 Fluorometer using the Qubit™ dsDNA BR (Broad Range) Assay Kit (Invitrogen, Carlsbad, CA, United States) following the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2021Quote: ... oocytes or embryos were cultured with O-propargyl-puromycin (OP-puro) (obtained as part of a Click-iT® Plus OPP Protein Synthesis Assay Kit; Molecular Probes/Thermo Fisher Scientific) at a concentration of 20 µM for the indicated time periods ...
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bioRxiv - Cancer Biology 2020Quote: ... and subject to qRT-PCR assays by the Power SYBR® Green RNA-to-Ct™ 1-Step Kit (Thermo Fisher Scientific, Waltham, MA) to measure molecule expression levels ...
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bioRxiv - Developmental Biology 2021Quote: ... OPP-Puro samples were processed for Click-chemistry detection with Alexa Fluor 647 picolyl azide as per the instructions in the Click-iT® Plus OPP Protein Synthesis Assay Kit (Invitrogen cat. no. C10458). Samples were then analyzed by flow cytometry ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The subset of individuals used in the 3RAD library prep (see below) were also quantified using the fluorescence-based Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen - Molecular Probes, Eugene, OR, USA). Extracted DNA were stored at −20°C until use.
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bioRxiv - Evolutionary Biology 2020Quote: Purified extracted DNA of modern samples was quantified with a Qubit 2.0 Fluorometer and dsDNA HS Assay kit (Life Technologies, Grand Island, NY, USA) and ca ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We quantified total RNA concentration (ng/μL) using a Qubit RNA HS assay kit on a Qubit 4 Fluorometer (Thermofisher Scientific, Waltham, MA, USA). We used a 4200 TapeStation Analyzer System and High Sensitivity RNA ScreenTape assays (Agilent ...
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bioRxiv - Genomics 2020Quote: ... RNA quality and concentration was then analysed on a Qubit® 2.0 Fluorometer using Qubit RNA BR assay kit (Invitrogen, Life Technologies, Carlsbad, CA, USA). Between 49 and 107 million sequence reads were generated for each sample on an Illumina HiSeq 4000 platform with a single-end sequencing primer over 150 cycles (Additional file 5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were spun down with a tabletop centrifuge and the protein concentration was determined using the BCA protein assay kit (Thermo Fisher Scientific, Cat: A532225) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Human DNA was removed using DNase I and RNA concentration was measured using Qubit RNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). DNA-depleted and purified RNA was used to construct double-stranded DNA library using MGIEasy RNA Library preparation reagent set (MGI ...
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bioRxiv - Genomics 2020Quote: ... and 4 μL was used for concentration measurement using the Qubit Fluorometer and Qubit dsDNA HS reagents Assay kit (Thermo Fisher Scientific, Waltham, MA). We finally combined the three extracts to reach DNA quantities sufficient for library preparation ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were centrifuged at 12,000 × g for 10 min at 4°C and the supernatant was isolated and quantified using a Pierce BCA protein assay kit (Thermo Fisher Scientific, MA, US). Loading buffer (4× ...
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bioRxiv - Molecular Biology 2019Quote: Whole-tissue sample lysates were batch process-assayed for total protein content using a BCA Protein Assay Kit (Thermo Fisher Scientific; Waltham, MA, USA). These lysates were used for Western blotting and enzyme assays described below.
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bioRxiv - Physiology 2019Quote: ... Purified RNA (100 ng per sample) was labeled with the Whole-Transcript Sense Target Assay kit (Affymetrix, Santa Clara, CA, USA; P/N 900652) and hybridized to an Affymetrix Mouse Gene 1.0 arrays or 2.1 ST array plate (Affymetrix) ...
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bioRxiv - Physiology 2019Quote: Total peroxide (PRX) content and total peroxidase (PER) activity was determined using the Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit (Invitrogen, Carlsbad, CA, USA) with slight modifications from the manufacturer’s protocol (de los Reyes et al. ...
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation was examined by 5-bromo-2-deoxyuridine (BrdU) cell proliferation enzyme-linked immunosorbent assay (ELISA) according to the kit manufacturer’s protocol (Life Technologies, Grand Island, NY, USA). The cells were incubated in presence of various concentrations of CLEFMA (0 – 10 µM) ...
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bioRxiv - Biochemistry 2020Quote: ... assay as determination of protein concentration by measurement of absorbance at 280 nm relies on the presence of tryptophan (Pierce™ BCA Protein Assay Kit-Reducing Agent Compatible; ThermoFisher Scientific; Waltham, MA).
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bioRxiv - Plant Biology 2021Quote: ... The final concentration of DNA samples was measured using Qubit 2.0 Fluorometer dsDNA BR Assay kits (Life Technologies-Thermo Fisher Scientific, Saint Aubin, France) and the final fragment size distribution using an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Plant Biology 2020Quote: ... Concentration and quality were analyzed using a NanoDrop™ 1000 spectrophotometer and a Qubit 4 Fluorometer with a dsDNA HS Assay Kit (ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Systems Biology 2021Quote: The H2O2 level in the extracellular medium was quantified with Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit (Invitrogen, Carlsbad, USA, Catalog no. A22188). 500 μL of cell suspension were spinned down after overnight growth in LB medium ...
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bioRxiv - Neuroscience 2020Quote: ... Lysate was centrifuged at 14,000g for 5min at 4°C and protein concentration was measured using Pierce BCA protein assay kit (Thermo Fisher Scientific, MA, USA PI23227) following manufacturer’s instructions ...